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Search Results: 1 - 10 of 244 matches for " Issei Imoto "
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Early Results of Omitting Completion Axillary Lymph Node Dissection in Sentinel Lymph Node Metastasis-Positive Breast Cancer Patients  [PDF]
Junko Honda, Hisashi Matsuoka, Chieko Hirose, Taeko Nagao, Takahiro Yoshida, Masako Takahashi, Issei Imoto, Mitsunori Sasa
Advances in Breast Cancer Research (ABCR) , 2013, DOI: 10.4236/abcr.2013.24021

Background: We investigated the early results of omitting completion axillary lymph node dissection (ALND) for axillary node metastasis-negative (N0), sentinel node (SN) metastasis-positive breast cancer patients. Patients and Methods: 489 patients had invasive N0 breast cancer treated without completion ALND, regardless of their SN metastasis status. Analyses included the associations between the SN metastasis status, clinicopathological findings and recurrence, between recurrence and clinicopathological findings, and recurrence-free survival. Results: 430 patients were SN biopsy (SNB)-negative, and 59 were SNB-positive. The SNB-positive patients received significantly more potent adjuvant therapy than the SNB-negative patients. Median follow-up was 3.7 years, and the axillary node recurrence was seen in 6 patients (1.2%) and recurrence in 21 patients. The SN status showed no associations with the clinicopathological findings or recurrence. Univariate analysis showed recurrence was associated with absence of hormonal therapy, ER-negative, PgR-negative, HER2-positive or triple-negative (TNBC) disease, a tumor ≥2.1 cm and higher nuclear grade. Multivariate analysis showed recurrence was associated with absence of hormonal therapy and a tumor ≥2.1 cm. Cox proportional hazards model showed recurrence was extremely early in ER-negative and TNBC patients. Conclusion: Completion ALND can be skipped in N0 breast cancer patients even if they are SNB-positive, but adjuvant therapy is essential.

NF90 in Posttranscriptional Gene Regulation and MicroRNA Biogenesis
Kiyoshi Masuda,Yuki Kuwano,Kensei Nishida,Kazuhito Rokutan,Issei Imoto
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms140817111
Abstract: Gene expression patterns are effectively regulated by turnover and translation regulatory (TTR) RNA-binding proteins (RBPs). The TTR-RBPs control gene expression at posttranscriptional levels, such as pre-mRNA splicing, mRNA cytoplasmic export, turnover, storage, and translation. Double-stranded RNA binding proteins (DSRBPs) are known to regulate many processes of cellular metabolism, including transcriptional control, translational control, mRNA processing and localization. Nuclear factor 90 (NF90), one of the DSRBPs, is abundantly expressed in vertebrate tissue and participates in many aspects of RNA metabolism. NF90 was originally purified as a component of a DNA binding complex which binds to the antigen recognition response element 2 in the interleukin 2 promoter. Recent studies have provided us with interesting insights into its possible physiological roles in RNA metabolism, including transcription, degradation, and translation. In addition, it was shown that NF90 regulates microRNA expression. In this review, we try to focus on the function of NF90 in posttranscriptional gene regulation and microRNA biogenesis.
Lysosomal-Associated Protein Multispanning Transmembrane 5 Gene (LAPTM5) Is Associated with Spontaneous Regression of Neuroblastomas
Jun Inoue, Akiko Misawa, Yukichi Tanaka, Shizuko Ichinose, Yuriko Sugino, Hajime Hosoi, Tohru Sugimoto, Issei Imoto, Johji Inazawa
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0007099
Abstract: Background Neuroblastoma (NB) is the most frequently occurring solid tumor in children, and shows heterogeneous clinical behavior. Favorable tumors, which are usually detected by mass screening based on increased levels of catecholamines in urine, regress spontaneously via programmed cell death (PCD) or mature through differentiation into benign ganglioneuroma (GN). In contrast, advanced-type NB tumors often grow aggressively, despite intensive chemotherapy. Understanding the molecular mechanisms of PCD during spontaneous regression in favorable NB tumors, as well as identifying genes with a pro-death role, is a matter of urgency for developing novel approaches to the treatment of advanced-type NB tumors. Principal Findings We found that the expression of lysosomal associated protein multispanning transmembrane 5 (LAPTM5) was usually down-regulated due to DNA methylation in an NB cell-specific manner, but up-regulated in degenerating NB cells within locally regressing areas of favorable tumors detected by mass-screening. Experiments in vitro showed that not only a restoration of its expression but also the accumulation of LAPTM5 protein, was required to induce non-apoptotic cell death with autophagic vacuoles and lysosomal destabilization with lysosomal-membrane permeabilization (LMP) in a caspase-independent manner. While autophagy is a membrane-trafficking pathway to degrade the proteins in lysosomes, the LAPTM5-mediated lysosomal destabilization with LMP leads to an interruption of autophagic flux, resulting in the accumulation of immature autophagic vacuoles, p62/SQSTM1, and ubiqitinated proteins as substrates of autophagic degradation. In addition, ubiquitin-positive inclusion bodies appeared in degenerating NB cells. Conclusions We propose a novel molecular mechanism for PCD with the accumulation of autophagic vacuoles due to LAPTM5-mediated lysosomal destabilization. LAPTM5-induced cell death is lysosomal cell death with impaired autophagy, not cell death by autophagy, so-called autophagic cell death. Thus LAPTM5-mediated PCD is closely associated with the spontaneous regression of NBs and opens new avenues for exploring innovative clinical interventions for this tumor.
NF-κB Inducing Kinase, a Central Signaling Component of the Non-Canonical Pathway of NF-κB, Contributes to Ovarian Cancer Progression
Masaya Uno, Yasunori Saitoh, Kanako Mochida, Eri Tsuruyama, Tohru Kiyono, Issei Imoto, Johji Inazawa, Yasuhito Yuasa, Toshiro Kubota, Shoji Yamaoka
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0088347
Abstract: Ovarian cancer is one of the leading causes of female death and the development of novel therapeutic approaches is urgently required. Nuclear factor-κB (NF-κB) is constitutively activated in several types of cancer including ovarian cancer and is known to support the survival of cancer cells. However, molecular mechanisms of persistent activation of NF-κB in ovarian cancer remain largely unknown. We report here that, in addition to the previously reported canonical activation, NF-κB is activated through the noncanonical pathway in ovarian cancer cells. RNA interference-mediated silencing of NF-κB inducing kinase (NIK), a central regulator of the noncanonical pathway, reduced the NF-κB2/p52 DNA binding activity and NF-κB-dependent reporter gene expression as well as NF-κB target gene expression. Notably, anchorage-dependent and -independent cell growth was impaired in NIK-depleted cells. Depletion of NIK also suppressed tumor formation in the nude mouse xenograft assay. These results indicate that NIK plays a key role in constitutive NF-κB activation and the progression of ovarian cancer cells and suggest that NIK represents an attractive therapeutic target for ovarian cancer.
Diagnosis and Prognostication of Ductal Adenocarcinomas of the Pancreas Based on Genome-Wide DNA Methylation Profiling by Bacterial Artificial Chromosome Array-Based Methylated CpG Island Amplification
Masahiro Gotoh,Eri Arai,Saori Wakai-Ushijima,Nobuyoshi Hiraoka,Tomoo Kosuge,Fumie Hosoda,Tatsuhiro Shibata,Tadashi Kondo,Sana Yokoi,Issei Imoto,Johji Inazawa,Yae Kanai
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/780836
Abstract: To establish diagnostic criteria for ductal adenocarcinomas of the pancreas (PCs), bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using 139 tissue samples. Twelve BAC clones, for which DNA methylation status was able to discriminate cancerous tissue (T) from noncancerous pancreatic tissue in the learning cohort with a specificity of 100%, were identified. Using criteria that combined the 12 BAC clones, T-samples were diagnosed as cancers with 100% sensitivity and specificity in both the learning and validation cohorts. DNA methylation status on 11 of the BAC clones, which was able to discriminate patients showing early relapse from those with no relapse in the learning cohort with 100% specificity, was correlated with the recurrence-free and overall survival rates in the validation cohort and was an independent prognostic factor by multivariate analysis. Genome-wide DNA methylation profiling may provide optimal diagnostic markers and prognostic indicators for patients with PCs.
Transcervical Interstitial Microwave Ablation Therapy for the Treatment of Adenomyosis: A Novel Alternative to Hysterectomy  [PDF]
Yasushi Kanaoka, Hirosumi Imoto
Open Journal of Obstetrics and Gynecology (OJOG) , 2014, DOI: 10.4236/ojog.2014.414118
Abstract: Objectives: To investigate the clinical effects of transcervical interstitial microwave irradiation of adenomyotic tissues as an alternative to hysterectomy. Methods: Thirty-three patients who were candidates for hysterectomy for the treatment of adenomyosis associated with menorrhagia were treated by interstitial microwave irradiation using a specifically developed transabdominal ultrasound probe attachment for transcervical puncture along with microwave endometrial ablation, as an alternative to hysterectomy. Primary outcomes were changes in the venous hemoglobin level and the volume of the uterine body before and after treatment. Secondary outcomes were the visual-analogue scale (VAS) score for menorrhagia and dysmenorrhea after treatment. Results: At 3 months after surgery, the average hemoglobin level significantly increased by 4.6 g/dL. The volume of the uterine body significantly decreased to 53% and 52% of the volume before treatment at 3 and 12 months, respectively. VAS scores indicated an improvement in menorrhagia and dysmenorrhea. Conclusions: Transcervical interstitial microwave ablation therapy combined with microwave endometrial ablation reduced the uterine body volume and relieved menorrhagia and dysmenorrheal caused by adenomyosis. This strategy is a promising alternative to hysterectomy for the treatment of symptomatic adenomyosis.
Analysis of a reduced-order HDG method for the Stokes equations
Issei Oikawa
Mathematics , 2015,
Abstract: In this paper, we analyze a hybridized discontinuous Galerkin(HDG) method with reduced stabilization for the Stokes equations. The reduced stabilization enables us to reduce the number of facet unknowns and improve the computational efficiency of the method. We provide optimal error estimates in an energy and $L^2$ norms. It is shown that the reduced method with the lowest-order approximation is closely related to the nonconforming Crouzeix-Raviart finite element method. We also prove that the solution of the reduced method converges to the nonconforming Gauss-Legendre finite element solution as a stabilization parameter $\tau$ tends to infinity and that the convergence rate is $O(\tau^{-1})$.
$K$-theory for the crossed products of infinite tensor product of $C^*$-algebras by the shift
Issei Ohhashi
Mathematics , 2015,
Abstract: C. Schochet shows K\"unneth theorem for the $C^*$-algebras in the smallest class of nuclear $C^*$-algebras which contains the separable Type I algebras and is closed under some operations. We calculate the $K$-theory for the crossed product of the infinite tensor product of a unital $C^*$-algebra in this class by the shift. In particular, we calculate the $K$-theory of the lamplighter group $C^*$-algebra.
Hybridized Discontinuous Galerkin Method with Lifting Operator
Issei Oikawa
Mathematics , 2013,
Abstract: In this paper, we propose a new hybridized discontinuous Galerkin method for the Poisson equation with homogeneous Dirichlet boundary condition. Our method has the advantage that the stability is better than the previous hybridized method. We derive $L^2$ and $H^1$ error estimates of optimal order. Some numerical results are presented to verify our analysis.
Hybridized discontinuous Galerkin method for convection-diffusion problems
Issei Oikawa
Mathematics , 2013,
Abstract: In this paper, we propose a new hybridized discontinuous Galerkin (DG) method for the convection-diffusion problems with mixed boundary conditions. A feature of the proposed method, is that it can greatly reduce the number of globally-coupled degrees of freedom, compared with the classical DG methods. The coercivity of a convective part is achieved by adding an upwinding term. We give error estimates of optimal order in the piecewise $H^1$-norm for general convection-diffusion problems. Furthermore, we prove that the approximate solution given by our scheme is close to the solution of the purely convective problem when the viscosity coefficient is small. Several numerical results are presented to verify the validity of our method.
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