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Search Results: 1 - 10 of 71937 matches for " Ismael Dale Cotrim Guerreiro da;Souza "
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Increased Risk of Acute Myeloid Leukemia in Patients with CYP1A1 Polymorphisms  [PDF]
Luís Arthur Flores Pelloso, Ismael Dale Cotrim Guerreiro da Silva, Naiara C?rrea Nogueira de Souza, Mihoko Yamamoto, Maria de Lourdes L. Ferrari Chauffaille
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.45111
Abstract:

Acute Myeloid Leukemia (AML) is a group of genetically diverse hematopoietic malignancies arising from cell progenitors developing in the myeloid pathway or from primitive stem cells. Genetic susceptibility of AML may account for an increased risk of AML due to partial metabolism of or biocativation of carcinogens. Chemical compounds are metabolized by a two-tiered phase detoxifying system. Polymorphisms in these pathways may lead to DNA damage and development of AML. We determined the frequencies of carcinogen metabolism gene polymorphisms (CYP1A1, del{GSTM1} and del{GSTT1}) in a case control-study based on polymorphism analysis. Fifty-eight consecutively AML patients (median age 62 years) and 174 sex and age-matched control group were assessed by a PCR-RFLP assay. There were 51 de novo and 7 secondary AML. CYP1A1*2A and CYP1A1*2C polymorphisms were more frequent in CG than AML p < 0.001 and in contrast, CYP1A1*3 and CYP1A1*4 were more frequent in AML than CG p <

Prevalence of Human Leukocyte Antigen HLA-B*5701 in HIV-1 Infected Individuals in Brazil  [PDF]
Claudinéia de Araújo, Cristina Valetta de Carvalho, Miriam Estela de Souza Freire, Amanda Yamaguti, Ivens Cuiabano Scaff, Fernando José de Souza, Flávia Galindo Silvestre Silva, Ricardo Sobhie Diaz, Ismael Dale Cotrim Guerreiro da Silva
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.41008
Abstract:

This study was designed to establish the prevalence of HLA-B*5701 at HIV-1 infected individuals in Brazil. A total of 517 consecutive individuals were followed in this study from February 2009 through July 2011. The presence of HLA-B*5701 was determined by Nested-PCR with HLA-B*57 and HLA-B*5701 sequence-specific primers (PCR-SSP). The expression of HLA-B*57 was negative in the 385 (74.5%) and positive in the 103 (19.9%) of infected individuals. Among these, the expression of HLA-B5701 was positive in the 29 (5.6%) of individuals. No demographic or ethnic differences were found between HLA-B*57/HLA-B*5701 HIV-1 negative patients, with a prevalence of Caucasians (57.1%) individuals. During the period of study, 68 patients were submited to an abacavir contain- ing regimen. The HLA-B*5701 allele was observed in 7 (10.3%) patients, with a significant incidence of Hypersensitivity reactions at 4 of them (p < 0.001). Conclusions: Although Brazilian population consists of a mixture of individuals of Caucasian, African and Native American genetic background, prevalence of HLA-B*5701 in this population is similar to the one found in pure Caucasians.


Gene Expression Analysis of Extracellular Matrix and Cytokines after Uterine Artery Embolization  [PDF]
André Bernardo, Tatiana Carvalho de Souza Bonetti, Ismael Dale Cotrim Guerreiro da Silva, Mariano Tamura Vieira Gomes, Rodrigo Aquino Castro, Manoel Jo?o Batista Castello Gir?o, Claudio Emilio Bonduki
Open Journal of Obstetrics and Gynecology (OJOG) , 2014, DOI: 10.4236/ojog.2014.415132
Abstract: Arterial embolization of myomas (AEM) is an established option for the conservative treatment of uterine leio-myomas; it treats all present uterine nodules at once, is less invasive than other procedures and effective in controlling symptoms, and does not require long term hospitalizations. Nevertheless, the potential impact on endometrial morphological and functional outcomes after the procedure is still controversial based on reports of endometritis or eventual transient ischemia. This study evaluated endometrial reorganization in uterine leiomyoma patients, before and after AEM, through gene expression analyses of extracellular matrix and cytokines genes in theendometrial tissue. Eight patients with leiomyomas were evaluated before AEM and 6 months after. The examinations included transvaginal pelvic ultrasonography, dosing of the follicle-stimulating hormone, and endometrial biopsy during the second phase of the menstrual cycle. RNA was extracted from endometrial samples, cDNA was synthesized, and applied on PCR arrayTM plates to evaluate the expression of extracellular matrix (ECM) genes and cytokines and their receptors’ genes (CYT). The ECM overexpressed genes were MMP (1, 3, 10, 11, and 14), CTGF1, ICAM1, TBHS1, ITGA2, ITGA3, ITGB3, COL7A1, COL12A, SPP1, and TNC; ADAMTS8 was underexpressed. The CYT overexpressed genes were SPP1, BCL6, CXCL12, IL-8, and CEBPB; CXCL13 and CCL21 were underexpressed. The ECM results showed overexpression of proteases that are responsible for dysfunctions in the ECM, and of genes responsible for adhesion and membrane components. The CYT results showed overexpression of chemokines responsible for endometrial repair, and underexpression of cytokines involved in inflammatory processes in the endometrial tissue. AEM treatment did not negatively affect the endometrial function at 6 months after embolization. This study broadens the knowledge about using a procedure that is relevant to the treatment of leiomyomas and contributes to the establishment of future guidelines for the decision making process for physicians and patients.
The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
Daniela Paes de Almeida Ferreira Braga,Tatiana Carvalho de Souza Bonetti,Ismael Dale Cotrim Guerreiro da Silva,Amanda Souza Setti
International Journal of Fertility & Sterility , 2013,
Abstract: Background: This study compares the developmental capacity of gametes retrieved from thelargest follicle with small follicles of a cohort in controlled ovarian stimulated cycles.Materials and Methods: This prospective study performed in a private assisted fertilizationcenter included 1016 follicles collected from 96 patients who underwent intra cytoplasmic sperminjection (ICSI). After follicular aspiration, oocytes were assigned to two groups accordingto the diameter of the derived follicle. The large follicle group (n=96) comprised oocytesderived from the leading follicle of the cohort and the small follicle group (n=920) consistedoocytes derived from the smaller follicles of the cohort. The fertilization and percentage of topqualityembryos were compared between groups by Chi-square or Fisher’s exact test, whereappropriate. The effect of the follicular diameter on oocyte dimorphism was assessed by binarylogistic regression.Results: A significantly higher percentage of oocytes derived from the leading follicle were inthe metaphase II (MII) stage (100 vs. 70.0%, p<0.001). However we observed no significantdifferences regarding the percentage of degenerated oocytes between the large (6.25%) and smallfollicle (5.0%) groups (p=0.550). Regression analysis demonstrated a nearly two-fold increase in theincidence of vacuoles in oocytes derived from the largest follicle of the cohort (OR: 1.81, p=0.046).The fertilization rate (50.0 vs. 38.8%, p=0.038) and the percentage of top quality embryos (84.7 vs.76.4%, p=0.040) were significantly higher for oocytes derived from the largest follicle. However,the percentage of abnormal fertilized oocytes was equally distributed between the large follicle(15.0%) and small follicle (12. 8%) groups (p=0.550).Conclusion: Our data suggest that intrafollicular mechanisms within the larger follicle of the cohortmay allow for these follicles to amplify the responsiveness to exogenous gonadotropin, which leads tothe formation of more competent oocytes with higher fertilization and developmental capacities.
Genetic polymorphism of GSTM1 in women with breast cancer and interact with reproductive history and several clinical pathologies
JOSé JUVENAL LINHARES,ISMAEL DALE COTRIM GUERREIRO DA SILVA,NAIARA C NOGUEIRA DE SOUZA,EMMANUELLE COELHO NORONHA
Biological Research , 2005,
Abstract: Due to the conflicting results regarding the association between breast cancerand the GSTM1 null mutation, our aim was to research this associationin a Brazilian population and correlations withsmoking, reproductive history and several clinical pathologies. A case-control study was performed on 105 women with breast cancer and 278 controls. Extraction of DNA was accomplished according to the protocol of the GFX kit and polymorphism analysis by the PCR technique. The control and experimental groups were compared and statistical analysis assessed by X2 or Fisher's exact test. The deletion in the GSTM1 gene in the breast cancer group had a prevalence of 32 (30.4%) individuals with the presence of null mutation. In the control group, the null mutation was present in 104 (37.4%) women. Upon comparison of the two groups, no statistically significant difference of the GSTM1 gene was observed, with an odds ratio (OR) of 0.74, 95%, confidence interval (CI) 0.45 - 1.20, p = 0.277. The results conclusively show that singlegene GSTM1 polymorphisms do not confer a substantial risk of breastcancer to its carriers. Furthermore, in this study no correlation was found between GSTs andsmoking, reproductive history and several clinical pathologies with respect to cancer risk.
Polimorfismo do gene do receptor de progesterona (PROGINS) em mulheres com cancer de mama: estudo caso-controle
Linhares, José Juvenal;Silva, Ismael Dale Cotrim Guerreiro da;Souza, Naiara Correa Nogueira de;Noronha, Emmanuelle Coelho;Ferraro, Odair;Baracat, Fausto Farah;
Revista Brasileira de Ginecologia e Obstetrícia , 2005, DOI: 10.1590/S0100-72032005000800007
Abstract: purpose: to analyze the correlation between progins polymorphism and breast cancer. methods: a case-control study was carried out from april to october 2004. the genotypes of 50 women with breast cancer and 49 healthy women were analyzed. the 306-base pair alu insertion polymorphism in the g intron of progesterone receptor gene was detected by polymerase chain reaction and analyzed on 2% agarose gel stained with ethidium bromide. the control and experimental groups were compared regarding genotypes using the statistical epi-info 6.0 program and for frequencies the exact fisher test or c2 test were used. p value smaller p than 5% was considered to be significant. results: in relation to progins we found in the studied population a prevalence of 62 (62.6%) wild homozygous, 35 (35.3%) heterozygous individuals and two (2.1%) cases with the presence of the mutation. regarding progins polymorphism, significance was not evidenced when cases and controls were compared, as related to homozygosis (62 vs 65.3%), heterozygosis (36 vs 34,6%) or the mutation (2.0 vs 2.1%), with p=0.920 (or=1.01), 0.891 (or=1.06), and 0.988 (or=1.10), respectively. conclusions: the results show that single-gene progins polymorphism does not confer a substantial risk of breast cancer to its carriers.
Genetic polymorphism of GSTM1 in women with breast cancer and interact with reproductive history and several clinical pathologies
LINHARES,JOSé JUVENAL; GUERREIRO DA SILVA,ISMAEL DALE COTRIM; NOGUEIRA DE SOUZA,NAIARA C; COELHO NORONHA,EMMANUELLE; FERRARO,ODAIR; DE CARVALHO,CRISTINA VALLETA; CHADA BARACAT,EDMUND; FARAH BARACAT,FAUSTO;
Biological Research , 2005, DOI: 10.4067/S0716-97602005000200017
Abstract: due to the conflicting results regarding the association between breast cancer and the gstm1 null mutation, our aim was to research this association in a brazilian population and correlations with smoking, reproductive history and several clinical pathologies. a case-control study was performed on 105 women with breast cancer and 278 controls. extraction of dna was accomplished according to the protocol of the gfx? kit and polymorphism analysis by the pcr technique. the control and experimental groups were compared and statistical analysis assessed by x2 or fisher's exact test. the deletion in the gstm1 gene in the breast cancer group had a prevalence of 32 (30.4%) individuals with the presence of null mutation. in the control group, the null mutation was present in 104 (37.4%) women. upon comparison of the two groups, no statistically significant difference of the gstm1 gene was observed, with an odds ratio (or) of 0.74, 95%, confidence interval (ci) 0.45 - 1.20, p = 0.277. the results conclusively show that single gene gstm1 polymorphisms do not confer a substantial risk of breast cancer to its carriers. furthermore, in this study no correlation was found between gsts and smoking, reproductive history and several clinical pathologies with respect to cancer risk.
Initial Analysis of Lipid Metabolomic Profile Reveals Differential Expression Features in Myeloid Malignancies  [PDF]
Adriana Ramos de Oliveira, Ismael Dale Cotrim Guerreiro Da Silva, Edson G. Lo Turco, Helio Alves Martins Júnior, Maria de Lourdes L. Ferrari Chauffaille Chauffaille
Journal of Cancer Therapy (JCT) , 2015, DOI: 10.4236/jct.2015.615138
Abstract: The purpose of this preliminary study was to determine the comparative lipid profile of blood plasma samples of healthy individuals and patients with Myeloproliferative Neoplasms. Methods: Untargeted Shotgun MS/MS Analysis was performed to evaluate plasma samples from 153 participants, being 90 of the Control Group, 43 Myeloproliferative Neoplasms (MPN), 11 Myelodysplastic Syndromes (MDS) and 9 Acute Myeloid Leukemias (AML). Lipids were extracted from plasma using the Bligh-Dyer protocol. Data were acquired using the AB-Sciex Analyst TF, processed using the AB-Sciex LipidViewTM and the web-based analytical pipeline MetaboAnalyst 2.0 (www.metaboanalyst.ca). Results: Untargeted analysis identified in negative and positive-modes a total of 658 features at 2 ppm resolution. PCA and PLS-DA analysis revealed clear discrimination among groups, in particular for AML patients. Main lipid groups differentially expressed were: Monoacylglycerols (MAG), Glucosylceramide E (GlcdE), Ethyl Esters (EE), Lysophosphatidic acid (LPA), Sulfoquinovosil diacylglycerols (SQDG), Monoglycerols (MG), Methyl Ethanolamines (ME), Lysophosphatidylcholines (LPC), Dimethyl Phosfatidyletanolamines (DMPE), Monometylphosphatidiletanolamines (MMPE), Ceramide-1-phosphate (CerP), Glicerophosphoglycerols (GP), Lysomonomethyl-Phosphatidylethanolamines (LMMPE), Phosphatidic Acids (PA), Ergosterols (ERG), Glycerophosphoserine (PS), Diacylglycerols (DAG), Hexocylceramides (HexCer) and Lanosterol (Lan). ROC Curve Analysis revealed Total LMMPE as the strongest discriminating marker between Controls from Patients. In addition, these lipids were also able to differentiate MDS and AML from NPM. Conclusions: The Myeloproliferative Neoplasms from the point of view
Associa??o entre polimorfismo SLC6A3 3?UTR VNTR e a resposta ao tratamento da dependência de nicotina
Focchi, Guilherme Rubino de Azevedo;Silva, Ismael Dale Cotrim Guerreiro da;Scivoletto, Sandra;
Jornal Brasileiro de Psiquiatria , 2011, DOI: 10.1590/S0047-20852011000400005
Abstract: objective: to evaluate the association between response to treatment of nicotine dependence with bupropion and the presence of the polymorphism slc6a3 3’utr vntr, in the gene that codifies the dopaminergic transporter. method: a hundred patients were treated in the nicotine dependence outpatient clinic of the institute of psychiatry, university of s?o paulo medical school. all patients were male, diagnosed as nicotine dependents and had no other diseases. all received bupropion until 300 mg a day for 12 weeks, combined with cognitive-behavioral group therapy. the fagerstr?m scale was applied at the beginning and at the end of treatment. cigarette cessation was evaluated in the last week of treatment and one month later. patients had 10 ml blood extracted and genotiped for slc6a3 3’utr vntr polymorphism. results: there was no association between cigarettes cessation and the presence of polymorphism. conclusion: more studies are needed to assess whether the presence of polymorphism slc6a3 3’utr vntr could be associated with a better response to treatment of nicotine dependence.
Associa??o entre os polimorfismos HaeIII e MspI do gene para o receptor alfa de estrogênio e densidade mamográfica em mulheres após a menopausa
Ramos, Eduardo Henrique de Moura;Kemp, Cláudio;Silva, Ismael Dale Cotrim Guerreiro da;
Revista Brasileira de Ginecologia e Obstetrícia , 2006, DOI: 10.1590/S0100-72032006001000003
Abstract: purpose: to assess the presence of estrogen receptor gene polymorphisms haeiii and mspi as well as clinical factors, and their possible associations with high mammographic density in post-menopausal women. methods: one hundred and fifteen post-menopausal women, not in use of hormonal therapy and without clinical or mammographic lesions were evaluated. three independent observers have determined the mammographic density pattern based on the acr-birads? 2003 (two subjective and one objective evaluations - adobe photoshop 7.0 software). oral swabs (cytobrush) were obtained to extract dna and the polymerase chain reaction - restriction fragment length polymorphism) was performed to assess the presence of polymorphisms in intron 1 and exon 1 from estrogen receptor gene (haeiii and mspi). results: the haeiii polymorphism was found in 43 (37.4%) of the 115 women, while mspi was found in 96 (83.5%) of them. there was a good agreement among determinations of the three observers with regard to mammographic density. thirty-four (29.6%) women had dense breasts and eighty-one (70.4%) had non-dense breasts. conclusion: the estrogen receptor gene polymorphism haelll showed no association with mammographic density (fisher = 0.712), while the association between estrogen receptor gene polymorphism mspl and mammographic density was near significance (fisher = 0.098). the associations among age, parity and body mass index revealed statistical significance.
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