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Search Results: 1 - 10 of 76927 matches for " Ismael Dale Cotrim Guerreiro da Silva "
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Increased Risk of Acute Myeloid Leukemia in Patients with CYP1A1 Polymorphisms  [PDF]
Luís Arthur Flores Pelloso, Ismael Dale Cotrim Guerreiro da Silva, Naiara C?rrea Nogueira de Souza, Mihoko Yamamoto, Maria de Lourdes L. Ferrari Chauffaille
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.45111

Acute Myeloid Leukemia (AML) is a group of genetically diverse hematopoietic malignancies arising from cell progenitors developing in the myeloid pathway or from primitive stem cells. Genetic susceptibility of AML may account for an increased risk of AML due to partial metabolism of or biocativation of carcinogens. Chemical compounds are metabolized by a two-tiered phase detoxifying system. Polymorphisms in these pathways may lead to DNA damage and development of AML. We determined the frequencies of carcinogen metabolism gene polymorphisms (CYP1A1, del{GSTM1} and del{GSTT1}) in a case control-study based on polymorphism analysis. Fifty-eight consecutively AML patients (median age 62 years) and 174 sex and age-matched control group were assessed by a PCR-RFLP assay. There were 51 de novo and 7 secondary AML. CYP1A1*2A and CYP1A1*2C polymorphisms were more frequent in CG than AML p < 0.001 and in contrast, CYP1A1*3 and CYP1A1*4 were more frequent in AML than CG p <

Initial Analysis of Lipid Metabolomic Profile Reveals Differential Expression Features in Myeloid Malignancies  [PDF]
Adriana Ramos de Oliveira, Ismael Dale Cotrim Guerreiro Da Silva, Edson G. Lo Turco, Helio Alves Martins Júnior, Maria de Lourdes L. Ferrari Chauffaille Chauffaille
Journal of Cancer Therapy (JCT) , 2015, DOI: 10.4236/jct.2015.615138
Abstract: The purpose of this preliminary study was to determine the comparative lipid profile of blood plasma samples of healthy individuals and patients with Myeloproliferative Neoplasms. Methods: Untargeted Shotgun MS/MS Analysis was performed to evaluate plasma samples from 153 participants, being 90 of the Control Group, 43 Myeloproliferative Neoplasms (MPN), 11 Myelodysplastic Syndromes (MDS) and 9 Acute Myeloid Leukemias (AML). Lipids were extracted from plasma using the Bligh-Dyer protocol. Data were acquired using the AB-Sciex Analyst TF, processed using the AB-Sciex LipidViewTM and the web-based analytical pipeline MetaboAnalyst 2.0 (www.metaboanalyst.ca). Results: Untargeted analysis identified in negative and positive-modes a total of 658 features at 2 ppm resolution. PCA and PLS-DA analysis revealed clear discrimination among groups, in particular for AML patients. Main lipid groups differentially expressed were: Monoacylglycerols (MAG), Glucosylceramide E (GlcdE), Ethyl Esters (EE), Lysophosphatidic acid (LPA), Sulfoquinovosil diacylglycerols (SQDG), Monoglycerols (MG), Methyl Ethanolamines (ME), Lysophosphatidylcholines (LPC), Dimethyl Phosfatidyletanolamines (DMPE), Monometylphosphatidiletanolamines (MMPE), Ceramide-1-phosphate (CerP), Glicerophosphoglycerols (GP), Lysomonomethyl-Phosphatidylethanolamines (LMMPE), Phosphatidic Acids (PA), Ergosterols (ERG), Glycerophosphoserine (PS), Diacylglycerols (DAG), Hexocylceramides (HexCer) and Lanosterol (Lan). ROC Curve Analysis revealed Total LMMPE as the strongest discriminating marker between Controls from Patients. In addition, these lipids were also able to differentiate MDS and AML from NPM. Conclusions: The Myeloproliferative Neoplasms from the point of view
Leptin Signaling Modulates Expression of Polycomb and Trithorax Complexes in the Brain of Fat Tissue Implanted Polycystic Ovarian Sindrome Mice  [PDF]
Eduardo Henrique da Silva Freitas, Samuel Marcos Ribeiro de Noronha, Maria Nazareth Gamboa Ritto, Carlos Fernandes Baptista, Ismael Dale Cotrim Guerreiro da Silva, Silvana Aparecida Alves Correa-Noronha, Ivaldo da Silva
American Journal of Molecular Biology (AJMB) , 2014, DOI: 10.4236/ajmb.2014.44020
Abstract: The Polycystic Ovary Syndrome (PCOS) is the most common androgenic disorder in women during reproductive life. PCOS may also be accompanied by metabolic syndrome and recent studies point to leptin as playing a role in disrupting infertility and in changing the energy balance in obese mice through its action on the hypothalamus. The aim is to assess the expression of the Polycomb & Trithorax Complexes genes in brain of mice transplanted with fat tissue from normal mice, in order to better understand the neuronal mechanisms underlying the reversion of PCOS. Three B6 V-Lepob/J mouse groups: Normal weight, obese and seven-day-treatment obese had their brain RNA extracted and submitted to an 84 Polycomb & Trithorax Complexes genes PCR Array plate and MetacoreTM pathways localization. Genomic profiles obtained were compared to the ones of the normal-weight-mice group. Differentially expressed genes were 13% and 26% respectively to control and treatment. Major changes were in genes: Snai1/31; Smarca1/?17; Dnmt3b/4.7; Ezh1/ 15. Altered genes were associated to canonical pathways and provided 3 networks related to epigenetics. Underlying neuronal changes caused by leptin in obese mice brain, there is an important role being played by the histone code. Here there is evidence that leptin drives the chromatin packing to a more condensed pattern. Upregulation of methyltransferase genes, like Ezh1, favors this thought. In summary the Polycomb & Trithorax complexes might answer for the silencing of some downregulated genes in the obese mice brain when exposed to leptin.
Associa??o entre polimorfismo SLC6A3 3?UTR VNTR e a resposta ao tratamento da dependência de nicotina
Focchi, Guilherme Rubino de Azevedo;Silva, Ismael Dale Cotrim Guerreiro da;Scivoletto, Sandra;
Jornal Brasileiro de Psiquiatria , 2011, DOI: 10.1590/S0047-20852011000400005
Abstract: objective: to evaluate the association between response to treatment of nicotine dependence with bupropion and the presence of the polymorphism slc6a3 3’utr vntr, in the gene that codifies the dopaminergic transporter. method: a hundred patients were treated in the nicotine dependence outpatient clinic of the institute of psychiatry, university of s?o paulo medical school. all patients were male, diagnosed as nicotine dependents and had no other diseases. all received bupropion until 300 mg a day for 12 weeks, combined with cognitive-behavioral group therapy. the fagerstr?m scale was applied at the beginning and at the end of treatment. cigarette cessation was evaluated in the last week of treatment and one month later. patients had 10 ml blood extracted and genotiped for slc6a3 3’utr vntr polymorphism. results: there was no association between cigarettes cessation and the presence of polymorphism. conclusion: more studies are needed to assess whether the presence of polymorphism slc6a3 3’utr vntr could be associated with a better response to treatment of nicotine dependence.
Associa??o entre os polimorfismos HaeIII e MspI do gene para o receptor alfa de estrogênio e densidade mamográfica em mulheres após a menopausa
Ramos, Eduardo Henrique de Moura;Kemp, Cláudio;Silva, Ismael Dale Cotrim Guerreiro da;
Revista Brasileira de Ginecologia e Obstetrícia , 2006, DOI: 10.1590/S0100-72032006001000003
Abstract: purpose: to assess the presence of estrogen receptor gene polymorphisms haeiii and mspi as well as clinical factors, and their possible associations with high mammographic density in post-menopausal women. methods: one hundred and fifteen post-menopausal women, not in use of hormonal therapy and without clinical or mammographic lesions were evaluated. three independent observers have determined the mammographic density pattern based on the acr-birads? 2003 (two subjective and one objective evaluations - adobe photoshop 7.0 software). oral swabs (cytobrush) were obtained to extract dna and the polymerase chain reaction - restriction fragment length polymorphism) was performed to assess the presence of polymorphisms in intron 1 and exon 1 from estrogen receptor gene (haeiii and mspi). results: the haeiii polymorphism was found in 43 (37.4%) of the 115 women, while mspi was found in 96 (83.5%) of them. there was a good agreement among determinations of the three observers with regard to mammographic density. thirty-four (29.6%) women had dense breasts and eighty-one (70.4%) had non-dense breasts. conclusion: the estrogen receptor gene polymorphism haelll showed no association with mammographic density (fisher = 0.712), while the association between estrogen receptor gene polymorphism mspl and mammographic density was near significance (fisher = 0.098). the associations among age, parity and body mass index revealed statistical significance.
Viscum Album Modulates Apoptotic Related Genes in Melanoma Tumor of Mice  [PDF]
Anamaria da Silva Facina, Gil Facina, Ismael Dale Cotrim Guerreiro da Silva, Giovana Aparecida Gon?alves, Fernando Augusto de Almeida, Silvana Aparecida Alves Corrêa de Noronha, Samuel Marcos Ribeiro de Noronha, Mary Uchiyama Nakamura
American Journal of Molecular Biology (AJMB) , 2014, DOI: 10.4236/ajmb.2014.42007

Cancer is a major public health problem throughout the world. It is estimated that one third of the American population will develop the disease at some time during their lifetimes. Among these, melanoma will account for 7% of the cases. In Brazil, in 2012, it is estimated that over six thousand new melanoma cases occurred. During recent years, the incidence of melanoma has increased, mainly due to a more constant exposure of the skin to sunlight. In this work, our aim is to assess the expression of apoptotis-related genes melanoma tumors in mice treated with Viscum album (VA). This will allow us to better understand the molecular mechanisms underlying tumor cell death activation caused by this compound. Our results clearly demonstrate upregulation of pro apoptotic genes (Trp53bp2, Nol3, Fadd, Tnfsf10, Traf1, Traf2, Cflar, Card10, Nod1, Casp 2, Casp7, Xiap, Dad1, and Dffb). Further bioinformatics-based tool analysis allowed us to assess which specific cell death-related intracellular pathways were activated by VA treatment. Two major effects of VA in melanoma cells could be observed: generation of

Angiotensin-(1-7) Changes Apoptosis-Related Genes Expression in Human Breast Cancer Cell Line T47D  [PDF]
Cheryl Alecrim Santos, Gabriela Soares da Silva Brito, Silvana Aparecida Alves Corrêa de Noronha, Samuel Marcos Ribeiro de Noronha, Suma Imura Shimuta, Clovis Ryiuchi Nakaie, Ismael Dale Cotrim Guerreiro da Silva
Journal of Cancer Therapy (JCT) , 2014, DOI: 10.4236/jct.2014.514143
Abstract: Angiotensin-(1-7) [Ang-(1-7)] is a heptapeptide of the renin-angiotensin system with vasodilator and anti-proliferative properties. In the present study, we aim to investigate whether Ang-(1-7) induces apoptosis in breast cancer cells and whether the altered expression of apoptosis-related genes is involved in this process. Human breast cell line T47D was treated with angiotensin-(1-7) and angiotensin II (Ang II). Cell proliferation and apoptosis were quantified using hemocytometer and flow cytometry, respectively. The expression of 84 apoptosis-related genes was evaluated through qPCR array. Ang-(1-7), as opposed to Ang II, decreased proliferation and increased apoptosis in T47D cells. Moreover, many pro-apoptotic genes were up-regulated, such as BAK1, BAX, BCL2L1, BID and BIK.
Prevalence of Human Leukocyte Antigen HLA-B*5701 in HIV-1 Infected Individuals in Brazil  [PDF]
Claudinéia de Araújo, Cristina Valetta de Carvalho, Miriam Estela de Souza Freire, Amanda Yamaguti, Ivens Cuiabano Scaff, Fernando José de Souza, Flávia Galindo Silvestre Silva, Ricardo Sobhie Diaz, Ismael Dale Cotrim Guerreiro da Silva
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.41008

This study was designed to establish the prevalence of HLA-B*5701 at HIV-1 infected individuals in Brazil. A total of 517 consecutive individuals were followed in this study from February 2009 through July 2011. The presence of HLA-B*5701 was determined by Nested-PCR with HLA-B*57 and HLA-B*5701 sequence-specific primers (PCR-SSP). The expression of HLA-B*57 was negative in the 385 (74.5%) and positive in the 103 (19.9%) of infected individuals. Among these, the expression of HLA-B5701 was positive in the 29 (5.6%) of individuals. No demographic or ethnic differences were found between HLA-B*57/HLA-B*5701 HIV-1 negative patients, with a prevalence of Caucasians (57.1%) individuals. During the period of study, 68 patients were submited to an abacavir contain- ing regimen. The HLA-B*5701 allele was observed in 7 (10.3%) patients, with a significant incidence of Hypersensitivity reactions at 4 of them (p < 0.001). Conclusions: Although Brazilian population consists of a mixture of individuals of Caucasian, African and Native American genetic background, prevalence of HLA-B*5701 in this population is similar to the one found in pure Caucasians.

Effects on Cell Viability and on Apoptosis in Tumoral (MCF-7) and in Normal (MCF10A) Epithelial Breast Cells after Human Chorionic Gonadotropin and Derivated-Angiotensin Peptides Treatments  [PDF]
Silvana Aparecida Alves Corrêa de Noronha, Werica Bernardo, Alexandre Jesus Barros, Clovis Ryuichi Nakaie, Suma Imura Shimuta, Ismael Dale Cotrim Guerreiro da Silva, Samuel Marcos Ribeiro de Noronha
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.47A010

Angiotensin-(1 - 7) [Ang-(1 - 7)] is an endogenous heptapeptide hormone of the renin-angiotensin system that has antiproliferative properties. The aim of this work was to evaluate the anti-proliferative and pro-apoptotic properties of Ang-(1 - 7) and of Ang-(1 - 7)-substituents 9-fluorenylmethyloxycarbonyl (Fmoc) e Ang II-derivatives containing the TOAC (2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid) in normal (MCF10A) and in tumoral (MCF7) epithelial mammary cell lines. Both cell lines received an hCG and angiotensin peptides 24-hour treatment, in combination or alone followed by cell viability, apoptosis and cell cycle assays performed by flow cytometer (GUAVA). After hCG, Ang-(1 - 7), hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments, MCF7 displayed cell viability decrease and mid-apoptosis increase. We also observed cell viability decrease in MCF10A after Ang-(1 - 7), Ang-(1 - 7) Fmoc and hCG + AngII Toac treatments. These cells had an increase in late apoptosis and necrosis after AngII Toac, hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments. Regarding the cell cycle analysis, we did not observed any changes in cell cycle phases. In summary, cell viability was decreased and apoptosis (initial, mid and late) was increased after hCG and/or Ang-(1 - 7) peptides treatments. These results point out hCG and Ang-(1 - 7) as effective compounds to inhibit cell proliferation, since they decrease cell viability and

Angiotensin-(1 - 7) and Human Chorionic Gonadotropin (hCG) Modulate the Nuclear Transcription Factors or Nuclear Receptors Genes in the Tumorigenic Undifferentiated Breast Cancer Cell Line SKBR3  [PDF]
Isidoro Binda Neto, Samuel Marcos Ribeiro de Noronha, Silvana Aparecida Alves Correa de Noronha, Maria Del Carmen Garcia Molina Wolgien, Alexandre Jesus Barros, Clovis Ryiuchi Nakaie, Suma Imura Shimuta, Gil Facina, Ismael Dale Cotrim Guerreiro da Silva
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.47A011

Breast cancer is the most common cancer among women. Angiotensin-(1 - 7) [Ang-(1 - 7)] has been correlated with cancer antiproliferative and apoptotic effects, similar properties of the human Chorionic Gonadotrofin (hCG). The aims of this work are to evaluate the role of Ang-(1 - 7) and of hCG in modulating the expression of Nuclear Receptors and Coregulators related genes in the tumorigenic breast cell line SK-BR3. Three experimental groups were created: control, hCG and hCG + Ang-(1 - 7). Cells were treated for 11 days and then had their RNA extracted. Samples were loaded into PCR Array plates containing 84 genes relate to Nuclear Receptors and Coregulators pathways. Gene expression data were used to construct canonical pathways (MetacoreTM). hCG and hCG + Ang-(1 - 7) treatments markedly modulate the expression of Nuclear Receptors and Coregulators related genes. hCG differentially expressed 17% of the genes, being 29% upregulated and 71% downregulated. Meanwhile, hCG + Ang-(1 - 7) changed the expression of 30% of the genes on the plate, among these genes 56% were upregulated and 44% downregulated. Among these differentially expressed genes, we highlight Esr1, Nr2f2, and Nr2f1, Esr1, Hdac5, and Nr4A1 (>4 fold). Finally MetaCore analysis based on Gene Ontology (GO) generated six networks for hCG and ten networks for the

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