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Search Results: 1 - 10 of 2934 matches for " Ishfak Hussain Wani "
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Evaluation of antimicrobial activity of a lectin isolated and purified from Indigofera heterantha  [PDF]
Sakeena Qadir, Ishfak Hussain Wani, Shaista Rafiq, Showkat Ahmad Ganie, Akbar Masood, Rabia Hamid
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.411133
Abstract:

Indigofera heterantha commonly called indigo bush is a member of leguminoseae family found in the Himalayan region of Kashmir. A lectin has been isolated from the seeds of Indigofera heterantha by the purification procedure involving anion exchange chromatography on DEAE-cellulose followed by gel filtration chromatography on Sephadex G 100. Molecular characterization of the lectin was done by gel filtration and SDS-PAGE. Activity of the lectin was checked by hemagglutination assay and the sugar specificity by sugar inhibition tests. The antimicrobial activity of the purified lectin was carried out by Agar disc diffusion using appropriate standards. On the ion exchange column, the bound protein when eluted with 0-0.5 M NaCl gradient emerged as three peaks—peak I, peak II and peak III out of which only peak II showed the hemagglutinating activity. The lectin further resolved into two peaks G1 and G2 on gel filtration, with the lectin activity residing in G1, corresponding to a molecular weight of 70 KDa. The purified lectin named as Indigofera heterantha Lectin (IHL) produced a single band on SDS PAGE (18 KDa), revealing the tetrameric nature of the lectin. It agglutinated human erythrocytes (A, B, AB, and O). Hemagglutination was inhibited by D-galactose, Dmannose and D-arabinose. The lectin is reasonably thermostable showing full activity within a temperature range of 30°C to 90°C. pH stability of the lectin falls in the range of 2-9. IHL demonstrated a remarkable antibacterial activity against the pathogenic bacteria Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. IHL also inhibited the growth

Inducing Fungus-Resistance into Plants through Biotechnology
Shabir Hussain WANI
Notulae Scientia Biologicae , 2010,
Abstract: Plant diseases are caused by a variety of plant pathogens including fungi, and their management requires the use of techniques like transgenic technology, molecular biology, and genetics. There have been attempts to use gene technology as an alternative method to protect plants from microbial diseases, in addition to the development of novel agrochemicals and the conventional breeding of resistant cultivars. Various genes have been introduced into plants, and the enhanced resistance against fungi has been demonstrated. These include: genes that express proteins, peptides, or antimicrobial compounds that are directly toxic to pathogens or that reduce their growth in situ; gene products that directly inhibit pathogen virulence products or enhance plant structural defense genes, that directly or indirectly activate general plant defense responses; and resistance genes involved in the hypersensitive response and in the interactions with virulence factors. The introduction of the tabtoxin acetyltransferase gene, the stilbene synthase gene, the ribosome-inactivation protein gene and the glucose oxidase gene brought enhanced resistance in different plants. Genes encoding hydrolytic enzymes such as chitinase and glucanase, which can deteriorate fungal cell-wall components, are attractive candidates for this approach and are preferentially used for the production of fungal disease-resistant plants. In addition to this, RNA-mediated gene silencing is being tried as a reverse tool for gene targeting in plant diseases caused by fungal pathogens. In this review, different mechanisms of fungal disease resistance through biotechnological approaches are discussed and the recent advances in fungal disease management through transgenic approach are reviewed.
Genetic Engineering for Viral Disease Management in Plants
Shabir Hussain WANI,Gulzar S. SANGHERA
Notulae Scientia Biologicae , 2010,
Abstract: Viral diseases are a major threat to world agriculture and breeding resistant varieties against these viruses is one of the major challenge faced by plant virologists and biotechnologists. The development of the concept of pathogen derived resistance gave rise to strategies ranging from coat protein based interference of virus propagation to RNA mediated virus gene silencing. Much progress has been achieved in protecting plants against these RNA and DNA viruses. In this review, the most recent transgene based approaches for viral disease management in plants will be discussed.
Sequence Characterization of Coding Regions of the Myostatin Gene (GDF8) from Bakerwal Goats (Capra hircus) and Comparison with the Sheep (Ovis aries) Sequence  [PDF]
Wani A. Ahad, Sajad A. Beigh, Mir Mehraj, Masroor Hussain, Hina F. Bhat, Riaz A. Shah, Ahmad S. Mudasir
Open Journal of Animal Sciences (OJAS) , 2016, DOI: 10.4236/ojas.2016.63020
Abstract: The Bakerwal breed of goat in Kashmir valley is a good meat purpose breed of goat. That attains an appreciable body weight under a low input production system. As these goats are mainly reared by Gujjar and Bakarwal tribes of the J & K state, so they usually are fed with the weeds, herbs, shrubs and grasses of pastures that will otherwise go waste. These goats constitute the main livestock wealth. With the good productive and reproductive potential, it makes these animals an important animal protein source for developing countries like India. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although several alterations in the intron and exon regions have been identified. The objective of our work is to characterize the myostatin coding regions using gene sequencing and polymerase chain reaction methods of Capra hircus (Bakerwal breed) and to compare them with the Ovis aries and other livestock species of animal, looking for variations in nucleotide and protein sequences. As mutations in the myostatin gene can inactivate its expression and result in a non-functional protein, which leads to increase in muscle growth in many species. In this way, we are able to identify 3 alterations on the presumed myostatin protein sequence as compared to non double-muscled ovine sequences.
Case Reports: Peritoneal hydatidosis in a young girl
I Wani, AM Lone, I Hussain, A Malik, M Thoker, KA Wani
Ghana Medical Journal , 2010,
Abstract: We report a case of peritoneal hydatidosis that occurred post laparotomy. Patient was diagnosed nine months after she had laparotomy for suspected acute appendicitis. The whole peritoneal cavity was studded with cysts. In view of diffuse involvement, patient was managed conservatively and showed response to medical therapy.
REE Characteristics and REE Mixing Modeling of the Proterozoic Quartzites and Sandstones  [PDF]
H. Wani
International Journal of Geosciences (IJG) , 2017, DOI: 10.4236/ijg.2017.81002
Abstract: Rare earth elements (REE) in sedimentary rocks are most suitable for source rock characterization. Rare earth element data of the sandstones of the unmetamorphosed Meso-Neoproterozoic Chhattisgarh and Indravati basins and the metamorphosed Paleoproterozoic Sakoli and Saucer basins of the Bastar craton have been studied for source rock characterization. The quartzites have higher ∑R EE mean value (145 ppm) compared to the sandstones (34 ppm). The REE patterns of all the three formations of the Chandarpur Group of the Chhattisgarh basin and the Tiratgarh Formation of the Indravati basin are uniform and there are no systematic differences in REE patterns among different formations of the Chandarpur Group and the Tiratgarh Formation. The REE patterns of the quartzites are similar to the REE patterns of the sandstones. Chondrite-normalized REE patterns with LREE enrichment and a strong negative Eu anomaly of the sandstones and quartzites gives a broad hint about felsic source rocks. The source rocks are identified as Archean granite and gneiss of the Bastar craton. The REE mixing modeling of the sandstones and quartzites suggest that the exposed the Proterozoic upper crust of the Bastar craton during the sedimentation of the Paleoproterozoic Sakoli and saucerand the Meso-Neoproterozoic Chhattisgarh and Indravati basins of the Bastar craton was largely consisted of gneissic rocks (70%), with a little contribution (20%) from Late Archean (2.5 Ga) granites. The present study does not suggest any significant change in the upper crustal composition during Proterozoic in the Bastar craton.
Open Underlay Mesh Reinforced Large Incisional Hernia Repair—Prospective Observational Hospital Based Study  [PDF]
Umer Mushtaq, Ishfaq Ahmad Gilkar, Javid Ahmad Peer, Shaukat Jeelani, Asgar Aziz, Farzanah Nowreen, Yaser Hussain Wani, Yaqoob Hassan, Drjavidahmad Peer
Surgical Science (SS) , 2019, DOI: 10.4236/ss.2019.103013
Abstract:
Introduction: Incisional hernias frequently complicate abdominal surgeries with a varied incidence as reported to be 2% - 20%. The risk factors of development of incisional hernias include immunocompromised state, diabetes mellitus, smoking, obesity, wound infection at the index surgery, emergency surgery. Materials and Methods: The study design was prospective and included 62 patients with incisional hernias. The patients were evaluated preoperatively on OPD basis with history, clinical examination, baseline investigation, ultrasound abdomen and computed tomogram. Results: The mean age was 48.9 years with male:female ratio of 1:1.4. Mean BMI was 30.1 kg/m2. Out of 62 patients in the study 61.2% had concomitant hypertension and were on treatment for the same. 30.6% were clinically hypothyroid, 38.7% were diabetic and 54.8% were smokers. There were multiple factors present in patients in the current study which were observed to be possible to have risked the patients to incisional hernias after an abdominal surgery. 21 patients had defect size ranging from 5 to 10 cm and 41 patients were bearing a hernia of the defect size of 10 cm or more. The mean operative time was 221.7 minutes. Conclusion: Incisional hernias are a part of surgical practice that would probably glue to it to the end of time. A progression from primitive suture repair with recurrence rates of over 65% to modern day mesh reinforced repairs with recurrences aimed at 0% is always welcome. But still then the placement of mesh in different positions or layers of abdominal wall yields different results.
Are health care systems insensitive to needs of suicidal patients in times of conflict? The Kashmir experience
Zaid Ahmad Wani,Arshad Hussain,Abdul Wahid Khan,Muhammad Maqbool Dar
Mental Illness , 2011, DOI: 10.4081/mi.2011.e4
Abstract: Suicide and attempted suicide is an important health issue and the number of people who die of suicide exceeds that of conflicts. Kashmir has been suffering from a low intensity war since last 20 years in which thousands have been killed or injured. There has been phenomenal increase in cases of psychological disorders along with suicide and and suicide attempters. Suicide in a conflict zone is viewed with indifference due to focus on the physical part of trauma. Difficulties faced by the suicidal patient and his attendants are seldom highlighted. 1408 patients who reported to emergency room for suicide attempt from 2000 to 2008 were taken for the study. All the patients underwent the hospital protocol for poisoning management. Patients were subjected to detailed psychiatric evaluation and questions were specifically asked about the difficulties encountered during management. Most of the cases were females with 92.11% belonging to the Muslim religion. 76.20% cases were from a rural background. 32.5% had been referred because of the lack of specific antidotes. Forty-three percent required arrangement of transportation by their own family members. Fifty-seven percent had been stopped for security checks along the way. Seventy-three percent felt that the attitude of the staff hostile. Twenty-three percent of patients had to share a bed. Almost all patients were questioned by security agencies within the hospital. More than 68% patient reported hostility amongst their neighbors. Suicidal poisoning is a significant health problem in Kashmir and management of these cases is fraught with difficulties across the spectrum of health care. Educating the doctors at primary care about first aid, improvement in community services followed by long term resolution of the conflict would go some way in alleviating the difficulties faced by a suicidal patient and his family in a conflict zone.
Stability Analysis for Yield and Yield Related Traits in Fodder Oats (Avena sativa L.)
F.A. Nehvi,Shafiq A. Wani,Altaf Hussain,M.I. Maqhdoomi
Asian Journal of Plant Sciences , 2007,
Abstract: Stability analysis was carried in twenty genotypes of fodder oats under four random environmental conditions of Kashmir valley. Observations were recorded on 10 maturity, morphological, quality, yield and yield related traits. Significant variation among genotypes was observed for all the traits indicating presence of high level of variability Significant GXE interaction was observed for all the traits. Partitioning of GXE interaction revealed that both the components were highly significant in all the characters with predominance of the Linear component, thereby, suggesting that the performance of genotypes across environments could be predicted with greater precission. Eleven genotypes showed average stability for green fodder yield of which nine genotypes (EC-3517, EC-35134, EC-13178, EC-1085898, EC-96848, EC-9865, EC-131532, Sabzaar and Kent) were well adopted and two genotypes (EC-54837 and EC-99170) were poorly adopted to all environments. Genotypes EC-35117, EC-35134, EC-13178, EC-1085898, EC-97248, EC-131532, Sabzaar and Kent were found to be stable and well adopted to all environments for most of the yield and yield related traits. These genotypes would be useful for commercial exploitation or can be exploited as elite gene pool in future breeding programme.
A clinical trial comparing parenteral oxytetracyline and enrofloxacin on time to recovery in sheep lame with acute or chronic footrot in Kashmir, India
J Kaler, S A Wani, I Hussain, S A Beg, M Makhdoomi, Z A Kabli, L E Green
BMC Veterinary Research , 2012, DOI: 10.1186/1746-6148-8-12
Abstract: There was significant correlation in recovery from lameness and presence of healing lesions in sheep with acute (r = 0.94) or chronic (r = 0.98) footrot. Sheep with acute footrot which were treated with parenteral antibacterials had a significantly more rapid recovery from lameness and had healing lesions (median = 7 days) compared with those treated with topical potassium permanganate solution (less than 50% recovered in 28 days). The median time to recovery in sheep with chronic footrot treated with either antibacterial was 17 days; this was significantly lower than the median of 75 days lame before treatment with antibacterials. The median time to recovery for both acute and chronic footrot increased as the severity of lesions increased. There was no difference in time to recovery by age, body condition score, duration lame, or presence of pus in the foot within acute and chronically affected sheep.We conclude that use of parenteral antibacterials to treat sheep lame with either acute or chronic footrot in India is highly effective. This is likely to improve welfare and give economic benefits to the farmers.India ranks sixth in the world in sheep production with an estimated 61.5 million sheep [1]. Sheep farming is the main livestock industry in northern hilly areas, such as Kashmir, where it serves as a major source of income for poor, rural communities who farm sheep for mutton and wool. The system of sheep rearing in Kashmir is semi-migratory. Flocks (20-30 sheep/flock) are kept housed by owners for 4-5 months during winter. In spring, they are grouped together with other flocks under the care of a "chopan" (shepherd), who takes them to graze high altitude sub-Himalayan and Himalayan pastures. In the autumn, sheep are walked back to the valley mostly by road and are returned to their owners [2].Footrot is an important, endemic disease of sheep in many countries including Australia, the UK, and New Zealand. Footrot is caused by Dichelobacter nodosus [3]. The cu
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