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Search Results: 1 - 10 of 391248 matches for " Isabel C.N.;Feio "
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Mucopolysaccharidoses in northern Brazil: Targeted mutation screening and urinary glycosaminoglycan excretion in patients undergoing enzyme replacement therapy
Viana, Gustavo M.;Lima, Nathália O. de;Cavaleiro, Rosely;Alves, Erik;Souza, Isabel C.N.;Feio, Raimunda;Leistner-Segal, Sandra;Schwartz, Ida;Giugliani, Roberto;Silva, Luiz C. Santana da;
Genetics and Molecular Biology , 2011, DOI: 10.1590/S1415-47572011005000025
Abstract: mucopolysaccharidoses (mps) are rare lysosomal disorders caused by the deficiency of specific lysosomal enzymes responsible for glycosaminoglycan (gag) degradation. enzyme replacement therapy (ert) has been shown to reduce accumulation and urinary excretion of gag, and to improve some of the patients' clinical signs. we studied biochemical and molecular characteristics of nine mps patients (two mps i, four mps ii and three mps vi) undergoing ert in northern brazil. the responsiveness of ert was evaluated through urinary gag excretion measurements. patients were screened for eight common mps mutations, using pcr, restriction enzyme tests and direct sequencing. two mps i patients had the previously reported mutation p.p533r. in the mps ii patients, mutation analysis identified the mutation p.r468w, and in the mps vi patients, polymorphisms p.v358m and p.v376m were also found. after 48 weeks of ert, biochemical analysis showed a significantly decreased total urinary gag excretion in patients with mps i (p < 0.01) and mps vi (p < 0.01). our findings demonstrate the effect of ert on urinary gag excretion and suggest the adoption of a screening strategy for genotyping mps patients living far from the main reference centers.
’n Calvinistiese kultuurbeskouing
C.N. Venter
Koers : Bulletin for Christian Scholarship , 1970, DOI: 10.4102/koers.v37i3&4.1315
Abstract: Ons sê met opset 'n Calvinistiese kultuurbeskouing, want daar is meer as een Calvinistiese kultuurbeskouing. In die ouere werke oor die geloofs- en sedeleer van die Christen ontbreek 'n selfstandige behandeling van kultuur meestal geheel en al. Die oorsaak hiervan lê waarskynlik daarin dat die kultuur as ’n gevaar vir ’n lewe in die vrese van die HERE beskou is. Die kultuur was van ,die wêreld’ en moes sover as moontlik vermy word — hoewel die Christen tog van die vrugte van ,die kultuur’ gebruik kon maak, want of hy nou ’n vreemdeling op aarde was, of ’n pelgrim na die ewigheid of ’n wagter op ’n wagpos op hierdie aarde, hy moes tog eet en drink en aantrek. Hy moes tog leef. Maar die basiese uitgangspunt was dat ,die kultuur’ net so wêrelds was as ,die wêreld’ self en dus sover moontlik gemy moes word.
Die verhale van kinders wat seksuele misbruik oorleef het: ’n Pastoraal-narratiewe ondersoek
C.N. Malan
HTS Theological Studies/Teologiese Studies , 2009, DOI: 10.4102/hts.v62i3.388
Abstract: The stories of children who survived sexual abuse: A pastoral-narrative inquiry This article presents the stories of two survivors. These stories show how the survivors were invited to capture “sparkling events” and developed them into preferred stories, and how by living in these stories, they could experience themselves in preferred and satisfactory ways. This article introduces various ways by which the news of empowerment and transformation can be circulated. The stories of these survivors were re-written and transformed by a community of shared experience, and it was through this experience that they could start dreaming about the transformation of their communities realizing how their stories and their journey had the ability to touch and transform the lives of numerous other survivors.
Het Afakaschrift in de Afrikanistiek
C.N. Dubelaar
Nieuwe West-Indische Gids , 1970,
Abstract:
Lijst van geschriften van Justus Wilhelm Gonggryp met beknopte biografie
C.N. Dubelaar
Nieuwe West-Indische Gids , 1970,
Abstract:
ANALYSIS OF IN VITRO ANTIBACTERIAL ACTIVITY OF SOME WEST INDIAN SPICES WITH THREE DIFFERENT SOLVENTS AGAINST S. TYPHI
C.N Khobragade
International Journal of Pharmaceutical Research and Development , 2010,
Abstract: Antibacterial activity of ten different spices extracts by using Distilled water, Ethanol and Ether were evaluated against Salmonella typhi using paper disc diffusion method. The antibacterial activity of all solvent extracts was positive for Salmonella typhi. The zone of inhibition in mm was varies with the type of spices and solvent. Ethanolic extract of Fenugreek, Ginger and Garlic inhibit growth of Salmonella typhi showing 12 mm, 10.5 mm and 10 mm diameter respectively. The Aqueous extract of Ginger, Coriander and Fenugreek inhibit Salmonella typhi showing 8 mm, 7.5 mm and 5.5 mm diameter respectively but in case of Ether extract Ginger, Fenugreek, Garlic and Coriander revealed 9.25 mm, 8.00 mm, 7 mm and 7 mm diameter respectively, though the Clove, Onion and Mustard do not shows any inhibitory effect on growth of Salmonella typhi for all solvent systems. The Minimum Inhibition Concentration (MIC) was observed in the range of 1000 mg/ml to 100 mg/ml concentrations, at any dilution Below 100 mg/ml concentration no inhibition was observed except for Clove, Onion and Mustard as they do not have any inhibition for solvent system except than Ethanol. Ethanolic extract had higher inhibitory effect as compared to other extracts in vitro against Salmonella typhi. There is a significant difference in weight of spices extracts after extraction and various color development with different solvents. Results indicates spices can be used as folk medicine and also as a natural coloring agent with different solvent system for various food preparations; the choice of solvent determines the degree of extraction, weight differences and color development after extraction with ethanol, ether and distilled water
Phosphorus Removal Capability of Aspergillus Terreus and Bacillus Subtilis from Nigeria’s Agbaja Iron Ore  [PDF]
C.N. Anyakwo, O.W. Obot
Journal of Minerals and Materials Characterization and Engineering (JMMCE) , 2010, DOI: 10.4236/jmmce.2010.912082
Abstract: Comparative phosphorus removal capability of a microbial fungus-Aspergillus terreus and a bacterium-Bacillus subtilis from Nigeria’s Agbaja iron ore was investigated. Manual crushing and sieving of the iron ore in Shital Test Kits produced 5 grain size distributions namely: ≥1.0mm, 1.0mm/0.50mm, 0.50mm/0.25mm, 0.25mm/0.125mm, 0.125mm/0.00mm, from which the first and last grain sizes were excluded from the experiment on technical grounds. Then 1g of each of the remaining 3 grain sizes was carefully weighed for each set of experiment. Culturing and subsequent inoculation of the microbes (which are native to the iron ore) in conical flasks containing sterilized, weighed iron ore samples in 100ml of equally sterilized malt extract and nutrient broths were left to stand for 7 weeks in the laboratory. At weekly interval, the samples were removed, treated through series of chemical reactions to obtain ammonium phosphomolybdate precipitate which was back-titrated with 0.1 N-HCl to determine the amount of phosphorus left in samples and consequently, the amount removed. The research found out Aspergillus terreus and Bacillus subtilis have separately and successfully removed phosphorusa deleterious non-metalic inclusion from Nigeria’s Agbaja iron ore, which previously defiled all attempts at phosphorous removal and beneficiation techniques. However their ability at phosphorus removal was promising and varied with the grain sizes of ore. Across all grain sizes used, 58% and 66% phosphorus was removed in 7 weeks, respectively by the two microbes. It was also obvious that the capacity of the microbes in removing phosphorus was seriously hampered by a decline in activity as indicated by the reduction in microbial population in the microcosm. For B. subtilis the density of viable cells increased from an initial load 3.4 x 105 cfu/ml, at the beginning of experiment, to 2.2 x 107 in 2 weeks but later declined to 0.9 x 106 cfu/ml at the end of 7 weeks. For A. terreus the initial biomass weight of 1g inoculated increased to 1.993 g dry weight within 3 weeks but later decreased to 0.910g dry weight in the 7th week. The reduction in microbial activity may be attributed to antimicrobial components of the ore, pyrite, and other heavy metals which may have affected the phosphorus uptake from ore. Our findings have confirmed the stronger phosphorus removal capability of B. subtilis under submerged culture technique. It is however suggestive that A. terreus may perform optimally if the submerged medium is constantly aerated.
Phosphorus Degradation Capability of Aspergillus terreus on Nigeria’s Agbaja Iron Ore  [PDF]
C.N. Anyakwo, O.W. Obot
Journal of Minerals and Materials Characterization and Engineering (JMMCE) , 2011, DOI: 10.4236/jmmce.2011.1012091
Abstract: A microbial fungus - Aspergillus terreus was used to degrade phosphorus in Nigeria’s Agbaja iron ore in the laboratory. The ore was first crushed to very tiny particles, screened using Shital test kits and 1.00/0.50mm, 0.50/0.25mm and 0.25/0.125mm particle size fractions were selected for the experiment. The microbes, obtained from the nascent remains on the ore environment, were cultured, used to inoculate 1g of sterile ore samples in 250ml conical flasks containing 100ml of equally sterile malt extract broth media and left to stand. At weekly interval, the samples were removed, treated through series of chemical reactions and ammonium phospho-molybdate precipitate was obtained. This was back-titrated with 0.1 NHCl to determine the amount of phosphorus left in samples and consequently, the amount removed. Findings reveal that A. terreus is capable of degrading the ore samples. pH monitoring reveals that the P degradation process proceeded in a culture media of increasing acidity. It is recommended to further study the chemistry of the mixture of culture media, ore samples and microbes to find parameters that favour the degradation process. Key words: Ore, microbes, screening, broth, degradation, accumulation, inoculati
The Microbiological and Physico-Chemical Analysis of Borehole Waters used by Off-Campus Students of Michael Okpara University of Agriculture, Umudike (MOUAU), Abia State, Nigeria
C.N. Obi,P. George
Research Journal of Biological Sciences , 2012, DOI: 10.3923/rjbsci.2011.602.607
Abstract: The microbiological and physico-chemical analysis of the seven borehole waters used by off-campus students of MOUAU were carried out using the Most Probable Number Technique (MPN) for the detection of faecal coliform. Five bacterial species Enterobacter aerogenes, Escherichia coli, Streptococcus faecalis, Staphylococcus aureus and Pseudomonas aeruginosa were isolated together with two fungal specie namely: Mucor racemosus and Aspergillus niger. The Total Heterotrophic Plate Count (THPC) gave a range of 4.7x104- 1.82x105 cfu mL-1 with a mean of 7.89x104 cfu mL-1 while the Total Coliform Plate Count (TCPC) gave a range of 4.1x103-8.6x103 cfu mL-1 with mean 6.24x103 cfu. The Presumptive faecal coliform ranged between 0-12 coliform per 100 mL with a mean of 3.57 coliform per 100 mL. Faecal coliform was detected in 57.1% of the water samples. The physico-chemical parameters fell within acceptable limits except for nitrate whose range fell between 65-20 mg L-1 above WHO guidelines of 10 mg L-1. The findings show that the water samples except those from AL and AG boreholes did not meet WHO standard for drinking. Thus, the bore-holes water and should be treated before drinking.
Microbiological Analyses of Drug Tablets from SelectedOutlets in Umuahia, Abia State, Nigeria
C.N. Ob,U. Nwannunu
Research Journal of Pharmacology , 2013, DOI: 10.3923/rjpharm.2010.31.37
Abstract: Six different types of drug tablets dispensed from containers in selected hospitals, community pharmacies and patent medicine stores were investigated for their microbial loads. Bacteria isolated include Bacillus sp., Staphylococcus aureus, Streptococci sp. and Escherichia coli, while the fungal isolates include Microsporum, Asperigillus, Trichophyton, Epidermatophyton and Penicillum sp. Results showed that samples collected from hospitals had the lowest microbial load compared to samples collected from community pharmacies and patent medicine stores. Drug samples from Hospital A had the highest Total Heterotrophic Bacterial Count (THBC) of 3.0x105 cfu mL-1, while Hospital B had the highest coliform count of 2.1x105 cfu mL-1. Pharmacy B had the highest THBC of 4.8x105 cfu mL-1, while Pharmacies B and C had the highest coliform count of 3.0x105 cfu mL-1, respectively. Patent Medicine Store D had the highest THBC of 5.7x105 cfu mL-1, while Patent Medicine Store B and D had the highest coliform counts of 3.0x105 cfu mL-1, respectively. The tablets analyses had high microbial loads, thus drug dispensers in hospitals, pharmacies and patent medicine stores should observe strict hygienic rules to reduce or eliminate contamination of the unsealed tablets given to the patients.
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