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Search Results: 1 - 10 of 69 matches for " Iracilda Zepone; "
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Biocompatibility of acellular dermal matrix graft evaluated in culture of murine macrophages
Vendramini, Ana Paula;Melo, Rafaela Fernanda;Marcantonio, Rosemary Adriana Chiérici;Carlos, Iracilda Zepone;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000200001
Abstract: the acellular dermal matrix allograft has been used as an alternative to autogenous palatal mucosal graft. the aim of this study was the evaluation of the biocompatibility of an acellular dermal matrix (alloderm?) in culture of macrophages. for hydrogen peroxidase determination we used the method of pick & kesari, and the griess method for nitric oxide determination,. statistical analysis showed no significant difference (p < 0,05) in the release of nitric oxide and hydrogen peroxide by the macrophages exposed to acellular dermal matrix and the negative control. the results suggest that acellular dermal matrix did not activate the cell inflammatory response.
Registro da ocorrência de Cybocephalus sp. (Coleoptera: Nitidulidae) predando espécies-praga de diaspididae (Hemiptera), no estado de Alagoas
LIMA, IRACILDA M.M.;
Neotropical Entomology , 2002, DOI: 10.1590/S1519-566X2002000100022
Abstract: specimens of cybocephalus sp. were collected preying on aspidiotus destructor (signoret) infesting coconut trees (dwarf cultivar) and on diaspis echinocacti (bouché) present on opuntia ficus-indica mill. and nopalea cochenillifera (l.) lyons (cactaceae). in the later case the nitidulid was associated with another predator: coccidophilus citricola (brèthes) (coleoptera: coccinellidae). the eggs were laid inside the scale of the diaspidid (one egg per scale); all life stages were detected. no sexual dimorphism was observed, and the natural sex ratio was 0.52 which represents a sexual proportion of 1.1 females : 1 male. cybocephalus specimens were also collected preying on coccids on alamanda cathartica l. (apocynaceae) and on morus alba l. (moraceae).
The morphology of the foregut of larvae and postlarva of Sesarma curacaoense De Man, 1892: a species with facultative lecithotrophy during larval development
Melo, Marlon Aguiar;Abrunhosa, Fernando;Sampaio, Iracilda;
Acta Amazonica , 2006, DOI: 10.1590/S0044-59672006000300014
Abstract: previous study on the resistance of larvae of sesarma curacaoense submitted to starvation has revealed a facultative lecithotrophy during zoeal stages, but megalopa and first juvenile stages are exclusively feeding stages. in the present study, the gross morphology and fine structure of the foregut of s. curacaoense were investigated during larval, megalopa and first juvenile stages. the foregut of the zoea i show specific setae and a filter press apparently functional. the foregut undergoes changes in the zoea ii (last larval stage) with increment of setae number, mainly on the cardiopyloric valve and complexity of the filter press. after metamorphosis to megalopa stage the foregut become rather complex, with a gastric mill supporting a medial and two lateral teeth well-developed. the foregut of the first juvenile is more specialized compared to the previous stage, showing similar characteristics of the decapod adults. these results provide further evidence of facultative lecithotrophic development in the larvae of s. curacaoense.
Novel 12S mtDNA findings in sloths (Pilosa, Folivora) and anteaters (Pilosa, Vermilingua) suggest a true case of long branch attraction
Barros, Maria Claudene;Sampaio, Iracilda;Schneider, Horacio;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008000400029
Abstract: we sequenced 12s rna mtdna for the majority of the extant species of sloths and anteaters and compared our results with previous data obtained by our group using 16s rna mtdna in the same specimens and to genbank sequences of the extinct giant sloth mylodon. our results suggest that pigmy-anteaters may be a case of the long-branch attraction phenomenon and also show the large genetic difference between the amazonian and atlantic forest three-toed sloths, contrasting with the small differences observed between the two non-atlantic forest forms of sloths. these results have important implications for the taxonomy of sloths and anteaters and strongly suggest the placement of pigmy anteaters in their own family (cyclopidae) and raising the taxonomic status of bradypus torquatus to a genus.
Genetic differentiation of Macrodon ancylodon (Sciaenidae, Perciformes) populations in Atlantic coastal waters of South America as revealed by mtDNA analysis
Santos, Sim?ni;Schneider, Horacio;Sampaio, Iracilda;
Genetics and Molecular Biology , 2003, DOI: 10.1590/S1415-47572003000200008
Abstract: the king weakfish (pescada-gó in portuguese - macrodon ancylodon (sciaenidae), a demersal (bottom-feeding) species found in south america atlantic coastal waters from the gulf of paria in venezuela to baia blanca in argentina, is an economically important species because of its abundance and wide acceptance by consumers. because of its wide distribution this fish may be subject to geographic isolation and this may have resulted in distinct populations along its coastal range. considering that this species represents an important economic resource, confirmation of whether m. ancylodon is a single species or there are different genetic stocks spread over its wide distribution would be an important contribution to conservation policies and population management of the king weakfish. to investigate differences between king weakfish populations we used the cytochrome b and 16s rrna genes to characterize m. ancylodon specimens caught throughout its south american range from venezuela to argentina. our results clearly distinguished two genetically different groups which show nucleotide divergence and genetic structuring patterns that strongly suggest they may be different species, disagreeing with the widely accepted traditional taxonomy that accepts only one species of macrodon in the western atlantic.
Phylogenetic analysis of 16S mitochondrial DNA data in sloths and anteaters
Barros, Maria Claudene;Sampaio, Iracilda;Schneider, Horacio;
Genetics and Molecular Biology , 2003, DOI: 10.1590/S1415-47572003000100002
Abstract: we sequenced part of the 16s rrna mitochondrial gene in 17 extant taxa of pilosa (sloths and anteaters) and used these sequences along with genbank sequences of both extant and extinct sloths to perform phylogenetic analysis based on parsimony, maximum-likelihood and bayesian methods. by increasing the taxa density for anteaters and sloths we were able to clarify some points of the pilosa phylogenetic tree. our mitochondrial 16s results show bradypodidae as a monophyletic and robustly supported clade in all the analysis. however, the pleistocene fossil mylodon darwinii does not group significantly to either bradypodidae or megalonychidae which indicates that trichotomy best represents the relationship between the families mylodontidae, bradypodidae and megalonychidae. divergence times also allowed us to discuss the taxonomic status of cyclopes and the three species of three-toed sloths, bradypus tridactylus, bradypus variegatus and bradypus torquatus. in the bradypodidae the split between bradypus torquatus and the proto-bradypus tridactylus / b. variegatus was estimated as about 7.7 million years ago (mya), while in the myrmecophagidae the first offshoot was cyclopes at about 31.8 mya followed by the split between myrmecophaga and tamandua at 12.9 mya. we estimate the split between sloths and anteaters to have occurred at about 37 mya.
Phylogenetic analysis of 16S mitochondrial DNA data in sloths and anteaters
Barros Maria Claudene,Sampaio Iracilda,Schneider Horacio
Genetics and Molecular Biology , 2003,
Abstract: We sequenced part of the 16S rRNA mitochondrial gene in 17 extant taxa of Pilosa (sloths and anteaters) and used these sequences along with GenBank sequences of both extant and extinct sloths to perform phylogenetic analysis based on parsimony, maximum-likelihood and Bayesian methods. By increasing the taxa density for anteaters and sloths we were able to clarify some points of the Pilosa phylogenetic tree. Our mitochondrial 16S results show Bradypodidae as a monophyletic and robustly supported clade in all the analysis. However, the Pleistocene fossil Mylodon darwinii does not group significantly to either Bradypodidae or Megalonychidae which indicates that trichotomy best represents the relationship between the families Mylodontidae, Bradypodidae and Megalonychidae. Divergence times also allowed us to discuss the taxonomic status of Cyclopes and the three species of three-toed sloths, Bradypus tridactylus, Bradypus variegatus and Bradypus torquatus. In the Bradypodidae the split between Bradypus torquatus and the proto-Bradypus tridactylus / B. variegatus was estimated as about 7.7 million years ago (MYA), while in the Myrmecophagidae the first offshoot was Cyclopes at about 31.8 MYA followed by the split between Myrmecophaga and Tamandua at 12.9 MYA. We estimate the split between sloths and anteaters to have occurred at about 37 MYA.
Genetic differentiation of Macrodon ancylodon (Sciaenidae, Perciformes) populations in Atlantic coastal waters of South America as revealed by mtDNA analysis
Santos Sim?ni,Schneider Horacio,Sampaio Iracilda
Genetics and Molecular Biology , 2003,
Abstract: The king weakfish (pescada-gó in Portuguese - Macrodon ancylodon (Sciaenidae), a demersal (bottom-feeding) species found in South America Atlantic coastal waters from the Gulf of Paria in Venezuela to Baia Blanca in Argentina, is an economically important species because of its abundance and wide acceptance by consumers. Because of its wide distribution this fish may be subject to geographic isolation and this may have resulted in distinct populations along its coastal range. Considering that this species represents an important economic resource, confirmation of whether M. ancylodon is a single species or there are different genetic stocks spread over its wide distribution would be an important contribution to conservation policies and population management of the king weakfish. To investigate differences between king weakfish populations we used the cytochrome b and 16S rRNA genes to characterize M. ancylodon specimens caught throughout its South American range from Venezuela to Argentina. Our results clearly distinguished two genetically different groups which show nucleotide divergence and genetic structuring patterns that strongly suggest they may be different species, disagreeing with the widely accepted traditional taxonomy that accepts only one species of Macrodon in the western Atlantic.
Registro de Plantas Hospedeiras (Cactaceae) e de Nova Forma de Dissemina??o de Diaspis echinocacti (Bouché) (Hemiptera: Diaspididae), Cochonilha-da-Palma-Forrageira, nos Estados de Pernambuco e Alagoas
LIMA, IRACILDA M.M.;GAMA, NOêMIA S.;
Neotropical Entomology , 2001, DOI: 10.1590/S1519-566X2001000300025
Abstract: opuntia ficus-indica mill. and nopalea cochenillifera (l.) lyons (cactaceae) are alternative food sources for cattle in drought areas of the northeastern region of brazil. diaspis echinocacti (bouché) is the unique pest of these cactacea species and it was previously detected on the following native host plants: cereus jamacaru dc., melocactus sp., opuntia dillenii haw. and o. inamoena k. schum. the new record of native hosts are cereus gounellei (weber) luetzelb., c. squamosus g?rke. and the exotic c. hildemannianus k. schum., from drought areas; pereskia grandifolia haw. and epiphyllum oxypetalum haw., native species from tropical forest used as ornamental plants. d. echinocacti has not infested o. monacantha haw. in the field and in laboratory bioessays. the dissemination of the neonate scale insect transported on the body surface of the predators chilocorus nigrita (fabricius) and zagreus bimaculosus (mulsant) coccinellidae: chilocorinae) was registered for the first time.
Molecular discrimination of pouched four-eyed opossums from the Mamirauá Reserve in the Brazilian Amazon
Nunes, Cláudia;Ayres, Jose Marcio;Sampaio, Iracilda;Schneider, Horacio;
Genetics and Molecular Biology , 2006, DOI: 10.1590/S1415-47572006000200015
Abstract: previous cytochrome b (cytb) mtdna studies have suggested four species for the opossum genus philander (four-eyed opossums), three (p. mcilhennyi, p. andersoni and p. opossum) from the amazon and one (p. frenata) from the brazilian atlantic forest. during a faunal survey nine specimens of philander sp. and four of didelphis marsupialis were collected in the mamirauá sustainable reserve, amazonas state, brazil. preliminary analyses based on morphology and geographical distributions were not conclusive, suggesting that philander specimens could belong to either p. andersoni or p. opossum. in order to elucidate the relationship of this taxon to the remaining amazonian taxa, seven philander and two didelphis specimens animals were sequenced for the cytb mtdna gene and compared to other previously studied taxa. the maximum likelihood (ml), neighbor-joining (nj) and maximum parsimony (mp) consensus bootstrap trees depicted six groups: didelphis., p. frenata, p andersoni, p. mcilhennyi, p.o. opossum and philander sp. and philander canus in a common assemblage supported by significant bootstrap values, suggesting that the philander sp. from mamiraua in fact belongs to the species philander canus.
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