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Search Results: 1 - 10 of 22922 matches for " Interferon-stimulated gene 15-kDa protein "
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Relationship between quantity of IFNT estimated by IFN-stimulated gene expression in peripheral blood mononuclear cells and bovine embryonic mortality after AI or ET
Shuichi Matsuyama, Takatoshi Kojima, Satoru Kato, Koji Kimura
Reproductive Biology and Endocrinology , 2012, DOI: 10.1186/1477-7827-10-21
Abstract: In the first experiment, IFNT (0, 500, or 1000 micrograms) was administered into the uterine horn ipsilateral to the CL 16 or 17 d after standing estrus, and mRNA levels of IFN-stimulated gene 15-kDa protein (ISG15) and Mx2 in peripheral blood mononuclear cells (PBMCs) were determined. In the second experiment, we investigated ISG15 mRNA expression in PBMCs during the MRP in cattle after either artificial insemination (AI) or embryo transfer (ET).Intrauterine administration of IFNT stimulated ISG15 and Mx2 gene expressions in PBMCs in cattle, and there was a positive correlation between the expressions of peripheral markers and the quantity of IFNT administered. In pregnant and normal interestrous interval (< 25 d) cattle (nIEI cattle), expression levels of the ISG15 gene showed similar patterns after AI and ET, and ISG15 mRNA expression was increased in pregnant cattle but unchanged in nIEI cattle. In contrast, ISG15 gene expression in extended interestrous interval (greater than or equal to 25 d) cattle (eIEI cattle) differed after ET compared with AI. In eIEI cattle after ET, ISG15 gene expression increased, such that the value on day 18 was intermediate between those of pregnant and nIEI cattle. In eIEI cattle after AI, ISG15 gene expression did not increase throughout the observation period.The results of the current study indicate that the quantity of conceptus-derived IFNT can be estimated by measuring ISG15 mRNA levels in PBMCs from cattle. Using this approach, we demonstrate that ISG15 gene expression during the MRP in eIEI cattle differed after ET compared with AI. In addition, the modest increase in ISG15 gene expression in eIEI cattle after ET suggests that late embryo losses were due to delayed or insufficient growth of the conceptus during the MRP in cattle.In most mammalian species, an embryo must signal its presence to the mother to establish a successful pregnancy. The signaling molecule in ruminants is interferon tau (IFNT), which is secreted from
Antiviral Type I and Type III Interferon Responses in the Central Nervous System
Frédéric Sorgeloos,Marguerite Kreit,Pascale Hermant,Cécile Lardinois,Thomas Michiels
Viruses , 2013, DOI: 10.3390/v5030834
Abstract: The central nervous system (CNS) harbors highly differentiated cells, such as neurons that are essential to coordinate the functions of complex organisms. This organ is partly protected by the blood-brain barrier (BBB) from toxic substances and pathogens carried in the bloodstream. Yet, neurotropic viruses can reach the CNS either by crossing the BBB after viremia, or by exploiting motile infected cells as Trojan horses, or by using axonal transport. Type I and type III interferons (IFNs) are cytokines that are critical to control early steps of viral infections. Deficiencies in the IFN pathway have been associated with fatal viral encephalitis both in humans and mice. Therefore, the IFN system provides an essential protection of the CNS against viral infections. Yet, basal activity of the IFN system appears to be low within the CNS, likely owing to the toxicity of IFN to this organ. Moreover, after viral infection, neurons and oligodendrocytes were reported to be relatively poor IFN producers and appear to keep some susceptibility to neurotropic viruses, even in the presence of IFN. This review addresses some trends and recent developments concerning the role of type I and type III IFNs in: i) preventing neuroinvasion and infection of CNS cells; ii) the identity of IFN-producing cells in the CNS; iii) the antiviral activity of ISGs; and iv) the activity of viral proteins of neurotropic viruses that target the IFN pathway.
Imunopatogênese da psoríase: revisando conceitos
Lima, Emerson de Andrade;Lima, Mariana de Andrade;
Anais Brasileiros de Dermatologia , 2011, DOI: 10.1590/S0365-05962011000600014
Abstract: insights into the pathogenesis of psoriasis led to the development of therapeutic tools aimed at blocking its immunological trigger. in parallel, cytokines such as the tumor necrosis factor (tnf) have been recognized as playing a crucial role in the pathogenesis of psoriasis and its associated comorbidities. genetic and immunological studies have contributed effectively towards establishing the currently held concepts regarding this complex disease.
Hepatitis C-Associated Mixed Cryoglobulinemic Vasculitis Induces Differential Gene Expression in Peripheral Mononuclear Cells
Sreetha Sidharthan,Xiaozhen Zhang,Shyam Kottilil
Frontiers in Immunology , 2014, DOI: 10.3389/fimmu.2014.00248
Abstract: This study examines the distinct gene expression profile of peripheral blood mononuclear cells from patients with chronic hepatitis C infection and mixed cryoglobulinemic (MC) vasculitis. Our DNA microarray analysis indicates that hepatitis C virus (HCV)-associated MC vasculitis is characterized by compromised neutrophil function, impaired chemotaxis, and increased interferon-stimulated gene (ISG) expression, contributing to overall MC pathogenesis and end-organ damage. Increased ISG expression is suggestive of an enhanced endogenous interferon gene signature. PBMC depletion assays demonstrate that this increased expression is likely due to an activation of monocytes and not a direct result of B cell expansion. Notably, this monocyte activation of ISG expression in HCV-associated MC vasculitis suggests a poor predictor status of interferon-based treatment. Further analysis of PBMC gene expression profiles before and after in vivo B cell depletion therapy is critical to completely understanding the mechanisms of MC vasculitis pathogenesis.
Selenoprotein-Transgenic Chlamydomonas reinhardtii
Qintang Hou,Shi Qiu,Qiong Liu,Jing Tian,Zhangli Hu,Jiazuan Ni
Nutrients , 2013, DOI: 10.3390/nu5030624
Abstract: Selenium (Se) deficiency is associated with the occurrence of many diseases. However, excessive Se supplementation, especially with inorganic Se, can result in toxicity. Selenoproteins are the major forms of Se in vivo to exert its biological function. Expression of those selenoproteins, especially with the application of a newly developed system, is thus very important for studying the mechanism of Se in nutrition. The use of Chlamydomonas reinhardtii ( C. reinhardtii) as a biological vector to express an heterogeneous protein is still at the initial stages of development. In order to investigate the possibility of using this system to express selenoproteins, human 15-KDa selenoprotein (Sep15), a small but widely distributed selenoprotein in mammals, was chosen for the expression platform test. Apart from the wild-type human Sep15 gene fragment, two Sep15 recombinants were constructed containing Sep15 open reading frame (ORF) and the selenocysteine insertion sequence (SECIS) element from either human Sep15 or C. reinhardtii selenoprotein W1, a highly expressed selenoprotein in this alga. Those Sep15-containing plasmids were transformed into C. reinhardtii CC-849 cells. Results showed that Sep15 fragments were successfully inserted into the nuclear genome and expressed Sep15 protein in the cells. The transgenic and wild-type algae demonstrated similar growth curves in low Se culture medium. To our knowledge, this is the first report on expressing human selenoprotein in green alga.
Fish interferon response and its molecular regulation: a review
鱼类干扰素反应及分子调控

Yibing Zhang,Jianfang Gui,
张义兵
,桂建芳

生物工程学报 , 2011,
Abstract: Interferon response is the first line of host defense against virus infection. Recent years have witnessed tremendous progress in understanding of fish innate response to virus infection, especially in fish interferon antiviral response. A line of fish genes involved in interferon antiviral response have been identified and functional studies further reveal that fish possess an IFN antiviral system similar to mammals. However, fish virus-induced interferon genes contain introns similar to mammalian type III interferon genes although they encode proteins similar to type I interferons, which makes it hard to understand the evolution of vertebrate interferon genes directly resulting in a debate on nomenclature of fish interferon genes. Actually, fish display some unique mechanisms underlying interferon antiviral response. This review documents the recent progress on fish interferon response and its molecular mechanism.
Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus

Jia Weizhang,Zhou Xiuxia,Huang Rong,Guo Qionglin,

自然科学进展 , 2007,
Abstract:
Virus-Heat Shock Protein Interaction and a Novel Axis for Innate Antiviral Immunity
Mi Young Kim,Michael Oglesbee
Cells , 2012, DOI: 10.3390/cells1030646
Abstract: Virus infections induce heat shock proteins that in turn enhance virus gene expression, a phenomenon that is particularly well characterized for the major inducible 70 kDa heat shock protein (hsp70). However, hsp70 is also readily induced by fever, a phylogenetically conserved response to microbial infections, and when released from cells, hsp70 can stimulate innate immune responses through toll like receptors 2 and 4 (TLR2 and 4). This review examines how the virus-hsp70 relationship can lead to host protective innate antiviral immunity, and the importance of hsp70 dependent stimulation of virus gene expression in this host response. Beginning with the well-characterized measles virus-hsp70 relationship and the mouse model of neuronal infection in brain, we examine data indicating that the innate immune response is not driven by intracellular sensors of pathogen associated molecular patterns, but rather by extracellular ligands signaling through TLR2 and 4. Specifically, we address the relationship between virus gene expression, extracellular release of hsp70 (as a damage associated molecular pattern), and hsp70-mediated induction of antigen presentation and type 1 interferons in uninfected macrophages as a novel axis of antiviral immunity. New data are discussed that examines the more broad relevance of this protective mechanism using vesicular stomatitis virus, and a review of the literature is presented that supports the probable relevance to both RNA and DNA viruses and for infections both within and outside of the central nervous system.
Cloning and Sequencing of 54kDa Fragment of 75kDa Readthrough Protein Gene from Beet Necrotic Yellow Vein Virus
甜菜坏死黄脉病毒75kDa通读蛋白基因54kDa片段的克隆及其序列分析

Yu Jialin Li Dawei Yang Lili Liu Yi,
于嘉林
,李大伟,杨莉莉,刘仪

生物工程学报 , 1995,
Abstract: 以甜菜坏死黄脉病毒(BNYVV)内蒙分离物的总RNA为模板,通过反转录和PcR扩增获得75kDa通读蛋白基因54kDa片段的目的片段。将其克隆到pGEM-7Zf(+)上并转化DH5α得到了含有完整s4kDa片段的重组子pGBW52。采用双脱氧终止法进行序列分析。结果表明内蒙分离物的54kDa片段全长为1509nt,与法国的F13分离物相比缺失了3个核苷酸。其核苷酸序列和由此推导的氨基酸序列的同源性分别为94.97%和96.42%.
Construction and Expression of 75kDa Readthrough Protein Gene from Beet Necrotic Yellow Vein Virus
甜菜坏死黄脉病毒75kDa通读蛋白基因构建与表达

Yu Jialin Li Dawei Liu Yi,
于嘉林
,李大伟,刘仪

生物工程学报 , 1995,
Abstract: Using DNA recombinant technique, coat protein (CP) gene and 54kDa fragment from beet necrotic yellow vein virus (BNYVV) RNA2 were ligated to construct 75kDa readthrough protein gene. Comparing with wild-typed 75kDa readthrough protein gene, 4 nucleotides of constructed 75kDa readthrough protein gene were replaced, including CP amber termination codon TAG to ATG. Corresponding, 2 amino acids were changed. The temperature-inducible expression vectors containing 75kDa readthrough protein gene or its 54kDa fragment were constructed and transformed into E. coli BL21 (DE3) respectively. The results of SDS-PAGE and Western blotting show: (1) specific expression of 75kDa readthrough protein gene was achiyed by temperature induction. Two smaller proteins, one is 37kDa. were also observted. (2) only a 37kDa protein from 54kDa fragment was expressed. This is the first report about specific expression of BNYVV 75kDa readthrough protein gene in E. coli.
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