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Search Results: 1 - 9 of 9 matches for " Immunodiagnostic "
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Detection of a Giardia lamblia coproantigen by using a commercially available immunoenzymatic assay, in Belo Horizonte, Brazil
ROCHA, Míriam Oliveira e;MELLO, R?mulo Teixeira de;GUIMAR?ES, Tania Mara Pinto Dabés;TOLEDO, Vicente de Paulo Coelho Peixoto de;MOREIRA, Maria da Concei??o Carneiro Gon?alves;COSTA, Carlos Alberto da;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1999, DOI: 10.1590/S0036-46651999000300003
Abstract: it is known that fecal examination to detect giardia lamblia cysts or trophozoites produces a high percentage of false-negative results. a commercially available immunoenzymatic assay (prospect giardia microplate assay, alexon, inc., biobrás) to detect g. lamblia specific coproantigen was evaluated for the first time in brazil. a total of 90 specimens were tested. each specimen was first tested as unpreserved stool, and then it was preserved in 10% formalin to be tested 2 months later. the assay was able to identify all the 30 positive patients (sensitivity = 100.0%) by visual or spectrophotometric examination in the unpreserved specimens and was negative in 57 of the 60 patients without g. lamblia (specificity = 95.0%). the assay identified 27 of the 30 positive patients (sensitivity = 90.0%) and was negative in 59 of the 60 negatives (specificity = 98.3%) in the preserved stools according to both readings. a marked difference was observed in the optical densities in both groups, preserved and unpreserved stools, when the g. lamblia-positive specimens were compared to the negative or positive for other intestinal parasites than g. lamblia. the assay seems a good alternative for giardiasis diagnosis, especially when the fecal examination was repeatedly negative and the patient presents giardiasislike symptoms.
Detection of a Giardia lamblia coproantigen by using a commercially available immunoenzymatic assay, in Belo Horizonte, Brazil
ROCHA Míriam Oliveira e,MELLO R?mulo Teixeira de,GUIMAR?ES Tania Mara Pinto Dabés,TOLEDO Vicente de Paulo Coelho Peixoto de
Revista do Instituto de Medicina Tropical de S?o Paulo , 1999,
Abstract: It is known that fecal examination to detect Giardia lamblia cysts or trophozoites produces a high percentage of false-negative results. A commercially available immunoenzymatic assay (ProSpecT Giardia Microplate Assay, Alexon, Inc., BIOBRáS) to detect G. lamblia specific coproantigen was evaluated for the first time in Brazil. A total of 90 specimens were tested. Each specimen was first tested as unpreserved stool, and then it was preserved in 10% Formalin to be tested 2 months later. The assay was able to identify all the 30 positive patients (sensitivity = 100.0%) by visual or spectrophotometric examination in the unpreserved specimens and was negative in 57 of the 60 patients without G. lamblia (specificity = 95.0%). The assay identified 27 of the 30 positive patients (sensitivity = 90.0%) and was negative in 59 of the 60 negatives (specificity = 98.3%) in the preserved stools according to both readings. A marked difference was observed in the optical densities in both groups, preserved and unpreserved stools, when the G. lamblia-positive specimens were compared to the negative or positive for other intestinal parasites than G. lamblia. The assay seems a good alternative for giardiasis diagnosis, especially when the fecal examination was repeatedly negative and the patient presents giardiasislike symptoms.
Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela
Gómez Martínez,Erika; Díaz-Bello,Zoraida; Zavala-Jaspe,Reinaldo; Tulio Díaz,Marcos; Noya,Oscar; Alarcón de Noya,Belkisyole;
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. an outbreak of paragonimus sp. in which didelphis marsupialis was the only wild reservoir incriminated was described in eastern venezuela. in order to have immunological tools to detect the presence of paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. didelphis marsupialis were captured in the locality of aguas blancas, montes municipality, sucre state, venezuela, from which blood samples were obtained and a search for worms was performed in lungs. worms were homogenized and ultracentrifugated to obtain fspa to perform immunoassay (elisa) to detect antibodies in opossums. the electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kda; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kda and 268 kda molecules being the most strongly recognized by positive sera. the negative sera did not recognize any band. the production of igy in chicken enabled the development of reagents capable of performing a standard immunodiagnosis technique to find specific anti-paragonimus sp. in didelphis marsupialis in order to establish epidemiological surveillance of these reservoirs in endemic areas.
Anti-Toxoplasma gondii secretory IgA in tears of patients with ocular toxoplasmosis: immunodiagnostic validation by ELISA
Lynch, Maria Isabel;Malague?o, Elizabeth;Lynch, Luiz Felipe;Ferreira, Silvana;Stheling, Raphael;Oréfice, Fernando;
Memórias do Instituto Oswaldo Cruz , 2009, DOI: 10.1590/S0074-02762009000600003
Abstract: toxoplasma gondii causes posterior uveitis and the specific diagnosis is based on clinical criteria. the presence of anti-t. gondii secretory iga (siga) antibodies in patients' tears has been reported and an association was found between ocular toxoplasmosis and the anti-t. gondii siga isotype in brazilian patients. the purpose of this study was to provide an objective validation of the published elisa test for determining the presence of anti-t. gondii siga in the tears of individuals with ocular toxoplasmosis. tears from 156 patients with active posterior uveitis were analysed; 82 of them presented characteristics of ocular toxoplasmosis (standard lesion) and 74 patients presented uveitis due to other aetiologies. cases of active posterior uveitis were considered standard when a new inflammatory focus satellite to old retinochoroidal scars was observed. the determination of anti-t. gondii siga was made using an elisa test with crude tachyzoite antigenic extracts. tears were collected without previous stimulation. detection of siga showed 65.9% sensitivity (95% ci = 54.5-74.4), 71.6% specificity (95% ci = 59.8-81.2), a positive predictive value of 72% (95% ci = 60.3-81.5) and a negative predictive value of 65.4% (95% ci = 54.0-75.4). siga reactivity was higher in the tears of patients with active posterior uveitis due to t. gondii (p < 0.05). the test is useful for differentiating active posterior uveitis due to toxoplasmosis from uveitis caused by other diseases.
Development of a Rapid Immunodiagnostic Test for Pork Components in Raw Beef and Chicken Meats: a Preliminary Study
S. N. Depamede
Media Peternakan , 2011,
Abstract: A rapid immunodiagnostic test that provides visual evidence of the presence of pork components in raw beef and chicken meats was developed. Colloidal gold was prepared and conjugated with anti-Swine IgG polyclonal antibody. Immunochromatographic test strips were produced, and then were used to test laboratory adulterated raw meat samples. The samples consisted adulteration meat, immunodiagnostic, pork, rapid test of pork-in-beef, or pork-in-chicken at 1/0; 1/100; 1/1,000; 1/5,000; 1/10,000 (w/w) adulteration levels that were extracted in phosphate-buffered saline. Raw beef and chicken meats without pork were included as controls. Analysis was completed in 10 min. Detection limit was 1/5,000 (w/w), although 1/10,000 was also observed. This immunodiagnostic tests can be conveniently applied to detect low levels of pork components in raw beef and chicken meat products. For the commercial purposes, further studies need to be carried out.
IMMUNOLOGYCAL APPROACH TO THE DIAGNOSIS OF AUTISM
Mirko SPIROSKI
Journal of Special Education and Rehabilitation , 2005,
Abstract: To date, neurotransmitter and immune abnormalities in autism are the only consistently documented findings other than neurobehavioral symptoms. Reciprocal communication between two major adaptive systems of human organism, the CNS and the immune system, is sustained via multiple pathways and is mediated by neurotransmitters, hormones, cytokines, chemokines, and corresponding cell receptors. Evidence for a dialogue between them has emerged as a universal concept of the neuroimmune regulation of homeostasis. In the paper we propose several plausible scenarios of how defects in either system may affect neuroimmune communications and lead to the pathology, as well as immunodiagnostic panels for recognition of immunopathological changes in people with autism in the Republic of Macedonia.
Entamoeba histolytica: fecal antigen capture immunoassay for the diagnosis of enteric amebiasis by a monoclonal antibody
Urdaneta, Haydeé;Rangel, Antonio;Martins, Maria Sonia;Mu?oz, Jose Francisco;Hernández M, Manuel;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1996, DOI: 10.1590/S0036-46651996000100008
Abstract: amebiasis continues to be of epidemiological importance in underdeveloped countries. clinical diagnosis and epidemiological setting in a region are based on the fecal microscopic identification of cysts or trophozoites. this procedure requires well trained personnel, is laborious, of low sensitivity and frequently yields false-positives results. the present study was designed to develop an immuno-enzymatic fecal 96 kda antigen capture test (coproelisa-eh) more sensitive and specific than microscopic diagnosis of amebiasis. triplicates of 177 stool samples processed by the formol-ether concentration method, were defined as positive or negative by three experienced microscopic observers. another aliquot was submitted to the antigen capture test by a monoclonal antibody against a specific membrane antigen of pathogenic strains of entamoeba histolytica. optical densities were interpreted as positive when they exceeded the mean value of negative samples plus two standard deviations. coproelisa-eh showed a 94.4% sensitivity, 98.3% specificity, 96.2% positive predictive value and 97.6% negative predictive value for the detection of e. histolytica in feces. coproelisa-eh is more sensitive and specific than microscopic examination, does not require specially trained personnel and allows the simultaneous processing of a large number of samples.
Entamoeba histolytica: detection of coproantigens by purified antibody in the capture sandwich ELISA
Urdaneta, Haidee;Guimar?es, Semíramis;Silva, Edward F.;Tavares, Carlos A. P.;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1994, DOI: 10.1590/S0036-46651994000600011
Abstract: a sensitive and specific capture sandwich elisa (cse) was developed using polyclonal purified rabbit antibodies against three different axenic strains of entamoeba histolytica: csp from brazil and hm1 - imss from mexico, for the detection of coproantigens in fecal samples. immunoglobulin g (igg) againstis e. histolytica was isolated from rabbits immunized with throphozoites whole extract in two stages: affinity chromatography in a column containing e. histolytica antigens bound to sepharose 4b was followed by another chromatography in sepharose antibodies 4b-protein a. a capture sandwich elisa using purified antibodies was able to detect 70ng of amebae protein, showing a sensitivity of 93% and specificity of 94%. the combination of microscopic examination and cse gave a concordance and discordance of 93.25% and 6.75%, respectively. it was concluded that cse is highly specific for the detection of coproantigens of e. histolytica in feces of infected patients, is quicker to perform, easier and more sensitive than microscopic examination.
Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela Immunodiagnostic in Didelphis marsupialis using an antigen of Venezuelan Paragonimus
Erika Gómez Martínez,Zoraida Díaz-Bello,Reinaldo Zavala-Jaspe,Marcos Tulio Díaz
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: Los Paragonimus son trematodos que habitualmente viven en los pulmones de mamíferos carnívoros y omnívoros, entre ellos el hombre. En el oriente venezolano se encuentra el único foco de Paragonimus sp. donde Didelphis marsupialis es el único reservorio demostrado hasta ahora. Con el fin de tener herramientas de inmunodiagnóstico que detecten la presencia de Paragonimus sp. en esta especie, se elaboraron varios reactivos para realizar un ensayo inmunoenzimático ELISA. Entre ellos se obtuvo un antígeno crudo soluble de vermes adultos de Paragonimus y una inmunoglobulina de gallina anti-IgG de Didelphis marsupialis. Los mismos se capturaron en la localidad de Aguas Blancas, municipio Montes, estado Sucre, Venezuela, y se obtuvieron muestras sanguíneas; en el caso de estar infectados, los vermes adultos se extrajeron del pulmón. Los parásitos se homogenizaron y ultracentrifugaron para obtener la fracción soluble del parásito (FSPA) como antígeno para el ELISA y Western blot y detectar los anticuerpos en los Didelphis marsupialis. El análisis electroforético mostró 22 moléculas entre 6 y 82 kDa; por Western blot se presentó un reconocimiento antigénico de 8 moléculas siendo las de 112 kDa y 268 kDa las más reconocidas por los sueros positivos. Los sueros negativos no reconocieron ninguna proteína. La producción de IgY en gallinas permitió desarrollar las técnicas de inmunodiagnóstico para la búsqueda de anticuerpos específicos anti-Paragonimus sp. en Didelphis, cuya aplicación permitirá establecer la vigilancia epidemiológica de estos reservorios en áreas endémicas sin sacrificio de los mismos. Paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. An outbreak of Paragonimus sp. in which Didelphis marsupialis was the only wild reservoir incriminated was described in eastern Venezuela. In order to have immunological tools to detect the presence of Paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. Didelphis marsupialis were captured in the locality of Aguas Blancas, Montes municipality, Sucre state, Venezuela, from which blood samples were obtained and a search for worms was performed in lungs. Worms were homogenized and ultracentrifugated to obtain FSPA to perform immunoassay (ELISA) to detect antibodies in opossums. The electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kDa; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kDa and 268 kDa molecules being the most strongly recognized by positive
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