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Search Results: 1 - 10 of 2317 matches for " IgG antibodies "
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Histone H1/MBP hydrolysing antibodies - novel potential marker in diagnosis of disease severity in systematic lupus erythematosus patients  [PDF]
Valentyna Chopyak, Yaroslav Tolstiak, Iryna Magoryvska, Rostyslav Bilyy, Rostyslav Bilyy, Natalya Korniy, Yuriy Kit, Rostyslav Stoika
Health (Health) , 2010, DOI: 10.4236/health.2010.210177
Abstract: Recently we have shown the presence of catalytically active IgGs, capable to cleave histone H1 and bovine myelin basic protein (MBP), in blood serum of SLE patients. Here we present data that demonstrate the correlation between a) proteolytic activity towards histone H1 and MBP of IgG-antibodies from blood serum of SLE patients and b) disease severity level in these patients. IgGs were isolated from blood serum by chromatography on protein G-sepharose. Commercial preparations of bovine myelin basic proteins (MBP) and calf thymus histone H1 were used as substrates. Analysis of the proteolytic activity showed that 16 of 38 lgG-preparations (42,1%) obtained from blood serum of SLE patients were capable of cleaving both histone H1 and MBP with different efficiency. It was revealed that the presence in blood serum of lgGs possessing proteolytic activity towards both histone H1 and bMBP closely correlates with manifestation of the disease severity in SLE patients.
Seroprevalencia del virus de la hepatitis A en ni?os de 1 a 15 a?os en un hospital universitario
Ospina,Sigifredo; Becerra,María Gabriela; Aguirre,Carlos; Mari?o,Ana Cristina; Galvis,Clara Esperanza; Villarreal,María Inés; De la Hoz,Fernando; Méndez,Hernando; Sierra,Alexandra; López,Pío; Pérez,Jorge; Niederbacher,Jurg; Espinal,Carlos; Mojica,Alejandro;
Infectio , 2011,
Abstract: introduction: hepatitis a is an infectious disease caused by a non-encapsulated rna virus of the picornaviridae family, classified as hepatovirus. it is transmitted by a fecal-oral route, either from person to person or in common source epidemics. objective: the aim of this study was to estimate the seroprevalence of igg antibodies against the hepatitis a virus in children aged 1-15 years, treated in a university hospital as part of a national collaborative study. methods: a descriptive study was performed between june and november 2007. the antibody titers were detected by means of a microparticle capture enzyme immunoassay. a survey to identify risk factors was conducted for each participant, with additional variables under study. results: we studied 422 children. the overall prevalence of antibodies against hepatitis a was 29.1%, with 37.1% in the group of 5 to 9 years of age, 36.1% for 10 to 15, and 13.8% for 1 to 4. the highest proportion of antibody prevalence was found in children of the lowest socioeconomic status, 44.2% for the stratum 1 and 27.9% for the stratum 2. conclusion: the seroprevalence to hepatitis a virus was lower in children with less than five years of age, which is an indication of a transition of the epidemiological profile to an intermediate one. the prevalence was higher in children of low socioeconomic levels, which may be related to overcrowding and poor hygiene practices
Investigation Correlates of Chlamydia Anti-Body Testing and Hysterosalpingography among Women with Tubal Infertility  [PDF]
Afolabi Korede Koledade, Adebiyi Gbadebo Adesiyun
Open Journal of Obstetrics and Gynecology (OJOG) , 2014, DOI: 10.4236/ojog.2014.416148

Background: Chlamydia trachomatis infection is an important preventable cause of infertility. In women, up to 70% of genital infection with Chlamydia trachomatis are asymptomatic. In the management of infertility patients, a lot of clinicians or centres do not routinely screen for Chlamydia trachomatis infection. Hence all patients being investigated for infertility may potentially be at risk of tubal blockage in addition to non-tubal factor aetiology. Those with primary tubal blockage also are at risk of worsening of the blockage. Objective: To determine if there is a relationship between IgG and IgM Chlamydia antibody testing (CAT) and tubal factor infertility. Design: It was a cross sectional descriptive study. Method: The sera of 400 consecutive consenting infertile patients presenting to the gynaecological clinic of the Ahmadu Bello University Teaching Hospital, Zaria, Northern Nigeria were tested for Chlamydia antibodies using ELISA IgG and IgM kits produced by Diagnostic Automation, Inc., 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302, USA. Results: Up to 264 (66%) of the patients had tubal factor, 64 (16%) had uterine, 56 (14%) had ovarian, 50 (12.5%) had male while 40 (10%) had others. The causative factors were not mutually exclusive. The sero-prevalence of IgG and IgMChlamydia trachomatis

Seroprevalencia de Toxoplasma gondii en donantes de sangre en la provincia de Guantánamo Seroprevalence of Toxoplasma gondii in blood donors from the province of Guantánamo
Rolando Sánchez Artigas,Walter Góngora Amores,Yordana Goya Batista,Antonio Miranda Cruz
Revista Cubana de Investigaciones Biom??dicas , 2012,
Abstract: Los riesgos de contaminación con Toxoplasma gondii a través de las transfusiones, están incrementados por la incidencia de este parásito en donantes y la tolerancia de este a los procesos de preparación y almacenamiento de la sangre, por lo que resulta peligroso para los grupos de riesgo. Esta enfermedad no es de declaración obligatoria en Cuba. El interés de conocer el comportamiento epidemiológico de esta parasitosis nos condujo a estudiar 562 muestras de donantes de sangre de la provincia de Guantánamo, donde resultaron positivos para IgG anti T. gondii el 47,0 %, con más del 62 % de positividad en los municipios de Baracoa y Maisí, con mayor prevalencia en áreas rurales. Los valores de seropositividad observados en los grupos etários estudiados y la relación entre sexo no fue significativa, aunque el contacto con el parásito en el grupo de edad entre 18 y 40 a os fue superior. Estos datos seroepidemiológicos alertan sobre la necesidad de proponer acciones de control en el uso de hemoderivados en los grupos de riesgo. The risk of contamination with Toxoplasma gondii via blood transfusion is increased by the incidence of this parasite in donors and its tolerance to blood processing and storage processes, which turns it into a hazard for risk groups. This disease is not notifiable in Cuba. The interest in learning about the epidemiological behavior of this parasitosis led us to study 562 blood donor samples from the province of Guantánamo, 47.0 % of which tested positive for anti-T. gondii IgG, with over 62 % positivity in the municipalities of Baracoa and Maisí, and higher prevalence in rural areas. Neither the seropositivity values found in the age groups studied nor their sex distribution were significant, though contact with the parasite was higher in the 18-40 age group. These seroepidemiological data point to the need to propose control actions for the use of blood products in risk groups.
Serological evidence of hantavirus infection in rural and urban regions in the state of Amazonas, Brazil
Gimaque, Jo?o Bosco Lima;Bastos, Michele de Souza;Braga, Wornei Silva Miranda;Oliveira, Cintia Mara Costa de;Castilho, Márcia da Costa;Figueiredo, Regina Maria Pinto de;Galusso, Elizabeth dos Santos;Itapirema, Evaulino Ferreira;Figueiredo, Luiz Tadeu Moraes de;Mour?o, Maria Paula Gomes;
Memórias do Instituto Oswaldo Cruz , 2012, DOI: 10.1590/S0074-02762012000100019
Abstract: hantavirus disease is caused by the hantavirus, which is an rna virus belonging to the family bunyaviridae. hantavirus disease is an anthropozoonotic infection transmitted through the inhalation of aerosols from the excreta of hantavirus-infected rodents. in the county of itacoatiara in the state of amazonas (am), brazil, the first human cases of hantavirus pulmonary and cardiovascular syndrome were described in july 2004. these first cases were followed by two fatal cases, one in the municipality of maués in 2005 and another in itacoatiara in 2007. in this study, we investigated the antibody levels to hantavirus in a population of 1,731 individuals from four different counties of am. sera were tested by igg/igm- enzyme-linked immune-sorbent assay using a recombinant nucleocapsid protein of the araraquara hantavirus as an antigen. ten sera were igg positive to hantavirus (0.6%). among the positive sera, 0.8% (1/122), 0.4% (1/256), 0.2% (1/556) and 0.9% (7/797) were from atalaia do norte, careiro castanho, itacoatiara and lábrea, respectively. none of the sera in this survey were igm-positive. because these counties are distributed in different areas of am, we can assume that infected individuals are found throughout the entire state, which suggests that hantavirus disease could be a local emerging health problem.
Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis
Barcelos, Ivanildes Solange da Costa;Moura, Leandro Pajuaba de;Costa, Vinicius Paulino da;Ferreira, Marcelo Sim?o;Costa-Cruz, Julia Maria;
Memórias do Instituto Oswaldo Cruz , 2007, DOI: 10.1590/S0074-02762007005000084
Abstract: the aim of this study was to test if serological distinction between patients with active and inactive neurocysticercosis (ncc), could be accomplished by the recognition of immunodominant peptides in total saline antigenic extract of taenia solium metacestodes by igg antibody in cerebrospinal fluid (csf) and serum paired samples. csf and serum samples of 10 each, active ncc patients, inactive ncc, and individuals with other neurological disorders, were used to recognize the antigenic peptides by western blot (wb). in the active ncc the 28-32 and 39-42 kda peptides were more frequently detected in csf than in sera (p < 0.05). the 47-52, 64-68, and 70 kda antigens showed high frequencies in both samples from patients with active ncc. all the csf samples of inactive ncc and other neurological disorder (control) patients tested negative, while serum samples from these last two groups recognized mainly the 80, 86, 95, and 98 kda bands. this finding eliminates the use of the high molecular weigh bands (> 80 kda) for diagnosis of ncc. the final conclusions were that the difference between active and inactive ncc may be done with the detection of peptides only in the csf samples and that the 47-52, 64-68, and 70 kda bands may be included as specific markers for active ncc when detected in csf samples by wb using total saline extract of t. solium metacestode.
Mechanisms of immune protection in the asexual blood stage infection by Plasmodium falciparum: analysis by in vitro and ex-vivo assays
Gysin, Jurg;Druilhe, Pierre;Silva, Luiz Pereira da;
Memórias do Instituto Oswaldo Cruz , 1992, DOI: 10.1590/S0074-02761992000900023
Abstract: mechanisms of immune protection against the asexual blood stage infection by plasmodium falciparum are reviewed. recent studies of two independent lines of research developed at the institute pasteur, in humans and primate infections clearly indicate an obligatory interaction of antibodies and effector cells to express the anti-parasitic effect.
Probable encefalitis por virus del oeste del Nilo
Díaz-Olachea, Carlos Gabriel;de la Maza-Flores, Manuel;Dondis-Cama?o, David;Lamas-Padilla, Biorica Elena;
Archivos de neurociencias (México, D.F.) , 2005,
Abstract: the case of a patient with a clinically suspected west nile virus encephalitis is described. the patient is a 70 year male with a 7 day fever who developed confusión, speaking difficulties and generalized muscular weakness. since the patient' s condition improved, he was discharged from this hospital seven days after admission. twenty days later, west nile virus igg antibodies were found high through elisa method. the case was retrospectively reviewed and it was classified as possible west nile virus encephalitis. the results if igg antibodies for west nile virus obtained by the elisa method, as well as the clinical features of the patient are consistent with west nile virus infection according to the cdc criteria.
Preparación de un conjugado peroxidasa-anti IgG humana (cadena ) en conejo
Merlín Linares,Julio C; Villaescusa Blanco,Rinaldo; Guerreiro Hernández,Ana M; González González,Juan M; Arce Hernández,Ada A;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2001,
Abstract: a peroxidase conjugate was prepared starting from the specific antibodies isolated from an anti-chain rabbit serum and from human igg. the specific antibodies were isolated by affinity chromatography and the conjugate was prepared by the method of oxidation with periodate. the conjugate obtained presented a molar igg/peroxidase relation of 1.07, a rz value of 0.33 and it was satisfactorily evaluated as regards its specificity and reactivity in the immunoenzimatic assays carried out
Antibody response in cattle after vaccination with inactivated and attenuated rabies vaccines
RODRIGUES da SILVA, Andréa de Cássia;CAPORALE, Graciane Maria Medeiros;GON?ALVES, Celso Alberto;TARGUETA, Mosar Couteiro;COMIN, Fabiano;ZANETTI, Carlos Roberto;KOTAIT, Ivanete;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2000, DOI: 10.1590/S0036-46652000000200006
Abstract: despite the absence of current official reports showing the number of cattle infected by rabies, it is estimated that nearly 30,000 bovines are lost each year in brazil. in order to minimize the important economic losses, control of the disease is achieved by eliminating bat colonies and by herd vaccination. in this study, we compare the antibody response in cattle elicited by vaccination with an attenuated era vaccine (aevac) and an inactivated-adjuvanted pv (ipvvac) vaccine. the antibody titers were appraised by cell-culture neutralization test and elisa, and the percentage of seropositivity was ascertained for a period of 180 days. ipvvac elicited complete seropositivity rates from day 30 to day 150, and even on day 180, 87% of the sera showed virus-neutralizing antibody titers (vna) higher than 0.5iu/ml. there were no significant differences between the vna titers and seropositivity rates obtained with ipvvac in the two methods tested. aevac, however, elicited significantly lower titers than those observed in the group receiving inactivated vaccine. in addition, the profiles of antirabies igg antibodies, evaluated by elisa, and vna, appraised by cell-culture neutralization test, were slightly different, when both vaccines were compared.
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