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Search Results: 1 - 10 of 461658 matches for " Ideris A "
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Gene expression profiles in primary duodenal chick cells following transfection with avian influenza virus H5 DNA plasmid encapsulated in silver nanoparticles
Jazayeri SD, Ideris A, Shameli K, Moeini H, Omar AR
International Journal of Nanomedicine , 2013, DOI: http://dx.doi.org/10.2147/IJN.S39074
Abstract: e expression profiles in primary duodenal chick cells following transfection with avian influenza virus H5 DNA plasmid encapsulated in silver nanoparticles Original Research (784) Total Article Views Authors: Jazayeri SD, Ideris A, Shameli K, Moeini H, Omar AR Published Date February 2013 Volume 2013:8 Pages 781 - 790 DOI: http://dx.doi.org/10.2147/IJN.S39074 Received: 12 October 2012 Accepted: 02 December 2012 Published: 21 February 2013 Seyed Davoud Jazayeri,1 Aini Ideris,1,2 Kamyar Shameli,3 Hassan Moeini,1 Abdul Rahman Omar1,2 1Institute of Bioscience, 2Faculty of Veterinary Medicine, 3Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Abstract: In order to develop a systemically administered safe and effective nonviral gene delivery system against avian influenza virus (AIV) that induced cytokine expression, the hemagglutinin (H5) gene of AIV, A/Ck/Malaysia/5858/04 (H5N1) and green fluorescent protein were cloned into a coexpression vector pIRES (pIREGFP-H5) and formulated using green synthesis of silver nanoparticles (AgNPs) with poly(ethylene glycol) and transfected into primary duodenal cells taken from 18-day-old specific-pathogen-free chick embryos. The AgNPs were prepared using moderated temperature and characterized for particle size, surface charge, ultraviolet-visible spectra, DNA loading, and stability. AgNPs and AgNP-pIREGFP-H5 were prepared in the size range of 13.9 nm and 25 nm with a positive charge of +78 ± 0.6 mV and +40 ± 6.2 mV, respectively. AgNPs with a positive surface charge could encapsulate pIREGFP-H5 efficiently. The ultraviolet-visible spectra for AgNP-pIREGFP-H5 treated with DNase I showed that the AgNPs were able to encapsulate pIREGFP-H5 efficiently. Polymerase chain reaction showed that AgNP-pIREGFP-H5 entered into primary duodenal cells rapidly, as early as one hour after transfection. Green fluorescent protein expression was observed after 36 hours, peaked at 48 hours, and remained stable for up to 60 hours. In addition, green fluorescent protein expression generally increased with increasing DNA concentration and time. Cells were transfected using Lipocurax in vitro transfection reagent as a positive control. A multiplex quantitative mRNA gene expression assay in the transfected primary duodenal cells via the transfection reagent and AgNPs with pIREGFP-H5 revealed expression of interleukin (IL)-18, IL-15, and IL-12β.
Gene expression profiles in primary duodenal chick cells following transfection with avian influenza virus H5 DNA plasmid encapsulated in silver nanoparticles
Jazayeri SD,Ideris A,Shameli K,Moeini H
International Journal of Nanomedicine , 2013,
Abstract: Seyed Davoud Jazayeri,1 Aini Ideris,1,2 Kamyar Shameli,3 Hassan Moeini,1 Abdul Rahman Omar1,21Institute of Bioscience, 2Faculty of Veterinary Medicine, 3Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, MalaysiaAbstract: In order to develop a systemically administered safe and effective nonviral gene delivery system against avian influenza virus (AIV) that induced cytokine expression, the hemagglutinin (H5) gene of AIV, A/Ck/Malaysia/5858/04 (H5N1) and green fluorescent protein were cloned into a coexpression vector pIRES (pIREGFP-H5) and formulated using green synthesis of silver nanoparticles (AgNPs) with poly(ethylene glycol) and transfected into primary duodenal cells taken from 18-day-old specific-pathogen-free chick embryos. The AgNPs were prepared using moderated temperature and characterized for particle size, surface charge, ultraviolet-visible spectra, DNA loading, and stability. AgNPs and AgNP-pIREGFP-H5 were prepared in the size range of 13.9 nm and 25 nm with a positive charge of +78 ± 0.6 mV and +40 ± 6.2 mV, respectively. AgNPs with a positive surface charge could encapsulate pIREGFP-H5 efficiently. The ultraviolet-visible spectra for AgNP-pIREGFP-H5 treated with DNase I showed that the AgNPs were able to encapsulate pIREGFP-H5 efficiently. Polymerase chain reaction showed that AgNP-pIREGFP-H5 entered into primary duodenal cells rapidly, as early as one hour after transfection. Green fluorescent protein expression was observed after 36 hours, peaked at 48 hours, and remained stable for up to 60 hours. In addition, green fluorescent protein expression generally increased with increasing DNA concentration and time. Cells were transfected using Lipocurax in vitro transfection reagent as a positive control. A multiplex quantitative mRNA gene expression assay in the transfected primary duodenal cells via the transfection reagent and AgNPs with pIREGFP-H5 revealed expression of interleukin (IL)-18, IL-15, and IL-12β.Keywords: silver nanoparticles, avian influenza, hemagglutinin, transfection, primary cells
Growth Performance and Blood Parameters as Influenced by Different Levels of Dietary Arginine in Broiler Chickens
M. Emadi,K. Kaveh,M.H. Bejo,A. Ideris,F. Jahanshiri,M. Ivan,R.A. Alimon
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2010.70.74
Abstract: An experiment was conducted to determine effects of dietary Arginine (ARG) on growth performance and blood serum parameters in broiler chickens. A corn-soybean meal based diet containing different levels of ARG (0, 0.67, 1.37, 2.07 and 2.77) for the starter (0, 0.53, 1.1, 1.68 and 2.25) for the grower and (0, 0.52, 1.04, 1.56 and 2.08) for the finisher was used. In a completely randomized design with five treatments of five replicates each and 10 chickens per replicate, 250 Cobb 500 male broiler chickens from 0-49 days of age were used. Growth performance (body weight gain, feed intake and feed: gain ratio) and blood serum (albumin, total protein, glucose, cholesterol, triglyceride, urea, uric acid, aspartate amino-transferase, alanine amino-transferase, alkalin phosphatase, lactic dehydrogenase and creatine kinase) parameters were measured at 27 and 49 days of age. Increase of dietary ARG increased (p<0.05) body weight gain, feed intake, albumin, creatine kinase, glucose, urea and uric acid and decreased (p>0.05) aspartate amino-transferase and cholesterol. It was concluded that dietary ARG might have positive effects on health status of the broiler chickens.
Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus
M. Emadi,F. Jahanshiri,F. Azizi Jalalian,K. Kaveh,M.H. Bejo,A. Ideris,A.A. Assumaidaee,R.A. Alimon
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2010.594.600
Abstract: Infectious Bursal Disease (IBD) continues to pose potential threat to poultry industry all over the world. The disease can spell disaster not only through its infection but also by break of immunity in chickens vaccinated for other diseases. On the other hand, arginine (Arg), a ubiquitous, semi-essential amino acid has emerged as an imunostimulant from variety of human and animal studies. In the present study, we demonstrate the stimulatory effects of Arg on systemic immune response in chickens challenged by orally administration of intermediate plus strain of IBD virus at 28 days of age. A corn-soybean meal based diet containing different levels of Arg (0, 0.67, 1.37, 2.07 and 2.77) for the starter, (0, 0.53, 1.1, 1.68 and 2.25) for the grower and (0, 0.52, 1.04, 1.56 and 2.08) for the finisher was used. In a completely randomized design with five treatments of five replicates each and 10 chickens per replicate, 250 Cobb500 male broiler chickens from 0-49 days of age were used. To measure the innate, cellular and humoral immunity indicators (interferon-α, interferon-γ, immunoglobulin G) at 27, 35, 42 and 49 days of age, serum samples from each replicate of treatments were collected and subjected to ELISA. The result showed that Arg supplementation in the chickens basal diets significantly increased the serum levels of interferon-α, interferon-γ, immunoglobulin G at 35, 42 and 49 days of age (p<0.05). The different levels of Arg at 27 days of age did not significantly affect interferon-α, interferon-γ, whereas Arg at 27 days of age significantly increased immunoglobulin G (p<0.05). These results revealed that arginine stimulates systematic immune response against intermediate plus strain of IBDV.
Attenuated Salmonella typhimurium SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens
Seyed Davoud Jazayeri,Aini Ideris,Zunita Zakaria,Abdul Rahman Omar
Journal of Biomedicine and Biotechnology , 2012, DOI: 10.1155/2012/264986
Abstract: Attenuated Salmonella has been used as a carrier for DNA vaccine. However, in vitro and in vivo studies on the bacteria following transfection of plasmid DNA were poorly studied. In this paper, eukaryotic expression plasmids encoding avian influenza virus (AIV) subtype H5N1 genes, pcDNA3.1/HA, NA, and NP, were transfected into an attenuated Salmonella enteric typhimurium SV4089. In vitro stability of the transfected plasmids into Salmonella were over 90% after 100 generations. The attenuated Salmonella were able to invade MCF-7 (1.2%) and MCF-10A (0.5%) human breast cancer cells. Newly hatched specific-pathogen-free (SPF) chicks were inoculated once by oral gavage with 109 colony-forming unit (CFU) of the attenuated Salmonella. No abnormal clinical signs or deaths were recorded after inoculation. Viable bacteria were detected 3 days after inoculation by plating from spleen, liver, and cecum. Fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) were carried out for confirmation. Salmonella was not detected in blood cultures although serum antibody immune responses to Salmonella O antiserum group D1 factor 1, 9, and 12 antigens were observed in all the inoculated chickens after 7 days up to 35 days. Our results showed that live attenuated S. typhimurium SV4089 harboring pcDNA3.1/HA, NA, and NP may provide a unique alternative as a carrier for DNA oral vaccine in chickens.
Molecular characterization of partial fusion gene and C-terminus extension length of haemagglutinin-neuraminidase gene of recently isolated Newcastle disease virus isolates in Malaysia
Ayalew Berhanu, Aini Ideris, Abdul R Omar, Mohd Bejo
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-183
Abstract: The coding region of eleven NDV isolates fusion (F) gene and carboxyl terminal region of haemagglutinin-neuraminidase (HN) gene including extensions were amplified by reverse transcriptase PCR and directly sequenced. All the isolates have shown to have non-synonymous to synonymous base substitution rate ranging between 0.081 - 0.264 demonstrating presence of negative selection. Analysis based on F gene showed the characterized isolates possess three different types of protease cleavage site motifs; namely 112RRQKRF117, 112RRRKRF117 and 112GRQGRL117 and appear to show maximum identities with isolates in the region such as cockatoo/14698/90 (Indonesia), Ch/2000 (China), local isolate AF2240 indicating the high similarity of isolates circulating in the South East Asian countries. Meanwhile, one of the isolates resembles commonly used lentogenic vaccine strains. On further characterization of the HN gene, Malaysian isolates had C-terminus extensions of 0, 6 and 11 amino acids. Analysis of the phylogenetic tree revealed that the existence of three genetic groups; namely, genotype II, VII and VIII.The study concluded that the occurrence of three types of NDV genotypes and presence of varied carboxyl terminus extension lengths among Malaysian isolates incriminated for sporadic cases.Newcastle disease (ND) is a highly contagious disease of birds and has been regarded throughout the world as one of the most important diseases of poultry and other birds [1], in which infection with the extremely virulent viruses may result in sudden, high mortality with comparatively few clinical signs. The causative agent, NDV, is avian Paramyxovirus under the Avulavirus and has a negative-sense, single-stranded RNA genome [2]. So far, NDV strains with genomic sizes of 15,186, 15192 and 15198 nucleotides which codes for at least six proteins including nucleoprotein (N), phosphoprotein (P), matrix (M) protein, fusion (F) protein, haemagglutinin-neuraminidase (HN) protein and RNA polymerase (L
The Importance of Managerial and Financial Control in Developmental Processes
Ahmed Saed Ahmed,Siti Rugayah Hj Tibek,Ideris Endot
International Business Management , 2012, DOI: 10.3923/ibm.2011.119.123
Abstract: The issue of development is not new, it has existed since a long period of time, an idea was born between two world wars and until now is still expanding. The concept of a comprehensive and contemporary process involving all levels and areas of life and developmental processes depend on several factors, their importance varies from one circumstance to another from one country to another and developmental processes are not automatic and easy. It takes a long time to operate and set up such operations, in fact, it is not only based on the foundations of material, proper planning is necessary and the availability of a broad inventory of the resources. Considering that people who are the makers of developmental processes are responsible for this planning but this does not mean that the role of material resources in the developmental processes should be ignored. Therefore, developing countries seek to find development resources on the basis of a comprehensive plan which addresses all sectors and ensure balance in the growth of these sectors. The process of developing plans for development does not guarantee that everything is fully in the institutions, therefore one must make sure that all activities of the research will be performed as specified in the plan. So, the control process is considered necessary and important for all types of institutions when exercising and performing other management functions such as planning, organization, leadership and decision-making so that the main objective of the control is to make sure that business is going in the direction of the goals. And that these goals are achieved at a high level of efficiency, competence and peaceful human relations, thus this study aims to identify the concept and objectives of management and financial controls also identify the concept of development objectives and the factors that help in the development process and levels of development and areas, the importance of oversight in the development processes, leading to these conclusions and recommendations.
Liquefaction Mechanisms and Mitigation-A Review
Nurmunira Binti Muhammad,Abdoullah Namdar,Ideris Bin Zakaria
Research Journal of Applied Sciences, Engineering and Technology , 2013,
Abstract: It is aimed to review a series of the research investigation on liquefaction mechanism for mitigation. A number of theoretical and computational studies have been performed by various researchers to determine the different types of liquefaction mechanism and evaluating ultimate bearing capacity of foundations in the presence of the static, dynamic and blast pore water pressure. But never these mechanisms have been compared base on latest scientific achievement. The liquefaction mechanisms of soil foundation under different condition have been reviewed by comparing experimental and numerical modeling for better interpretation. The result has been highlighted that the water pressure function could be controlled without reducing pore water pressure magnitude. This guideline could be used for liquefaction mitigation. The research requirement is also recommended.
Dissemination of Newcastle Disease Virus (NDV-AF2240) in Liver during Intratumoral Injection of Xenotransplant Breast Cancer in BALB/c Mice
Gholamreza Motalleb,Fauziah Othman,Aini Ideris,Asmah Rahmat
Cell Journal , 2009,
Abstract: Objective: Newcastle disease virus (NDV) or avian paramyxovirus type1 possessesseveral unique properties that make it an excellent anticancer agent. The hemagglutininneuraminidase (HN) protein of NDV plays an important role in viral infection. Thepurpose of the present study is to investigate the dissemination of Newcastle diseasevirus (NDV) AF2240 strain in the liver during intratumoral injection in 4T1 breast cancerin female BALB/c mice.Materials and Methods: A total of 200 female BALB/c mice were divided randomlyinto 10 cancerous groups consisting of 20 mice per group. The mice were initially inducedwith 104 4T1 cells, NDV-AF2240 and tamoxifen co-culture. Cancerous groupswere divided into: cancer control (CC), cancer treated with 0.5 μg/ml tamoxifen citrate(CT), cancer treated with 8, 16, 32 and 64HA units of NDV-AF2240 (respectivelynamed C/NDV8, C/NDV16, C/NDV32, C/NDV64), cancer treated with 8, 16, 32 and64HA units of NDV-AF2240 and tamoxifen (respectively as CT/NDV8, CT/NDV16, CT/NDV32 and CT/NDV64 daily for four weeks). In situ reverse transcription polymerasechain reaction (In situ RT-PCR), negative staining electron microscopy (NSEM), polyclonalchicken antibody and goat anti-chicken antibody conjugated with fluoresceinisothiocynate (FITC) using confocal laser scanning microscopy (CLSM) were used todetect the virus in the tumor and liver.Results: In situ RT-PCR, NSEM and CLSM successfully detected NDV-AF2240 intumor cells and liver.Conclusion: The findings showed NDV-AF2240 disseminated into liver during intratumoralinjection.
Oncolytic Effect of Newcastle Disease Virus AF2240 Strain on the MCF-7 Breast Cancer Cell Line
Fauziah Othman,Aini Ideris,Gholamreza Motalleb,Zulkapli Bt. Eshak
Cell Journal , 2010,
Abstract: Objective: This study was carried out to investigate the oncolytic effect of the Newcastledisease virus (NDV) strain AF2240 on the MCF-7 breast cancer cell line.Materials and Methods: The NDV-AF2240 was propagated in 11 days old embryonatedchicken eggs for 72 hours. The virus in the allantoic fluid was harvested andpurified. The haemagglutination (HA) test was conducted on the purified virus to determinethe virus titre which was 16384 haemagglutination units (HAUs). The microculturetetrazolium assay (MTA) was carried out via two methods-the monolayer and co-culturetechniques- to determine the inhibitory concentration (IC50) of NDV-AF2240 against theMCF-7 breast cancer cell line. Confocal laser scanning microscopy was carried out onpolyclonal chicken antibody and fluorescein isothiocynate (FITC) conjugated goat antichickenantibody to observe virus localization in the cells. The terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL) assay was conducted to quantifythe percentage of apoptotic cells.Results: IC50 value of NDV-AF2240 was two HAUs in both the monolayer and co-cultures.Virus particles were detected in the cytoplasm of MCF-7 breast cancer cell lineafter 24 and 48 hours post treatment. Virus budding was detected 72 hours post treatment.The number of apoptotic cells was significantly increased (p<0.05) 72 hours postNDV-AF2240 treatment.Conclusion: The findings of this study show that NDV-AF2240 has an oncolytic effectagainst the MCF-7 breast cancer cell line. Further studies are needed to understand theanti cancer mechanism of this virus.
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