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Search Results: 1 - 10 of 46516 matches for " Hyun-Jin Kim "
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Clinical Pharmacokinetic and Bioequivalence Studies of Two Brands of Cephradine in Healthy Korean Using HPLC Method  [PDF]
Hyun-Jin Kim, Shin-Hee Kim, Semi Kim, Jae-Sung Ahn, Ju-Seop Kang
Pharmacology & Pharmacy (PP) , 2018, DOI: 10.4236/pp.2018.97022
Abstract: The goal of our research was to compare the pharmacokinetics and evaluate the bioequivalence of two brands of cephradine 500 mg capsules in 24 normal Korean volunteers. The plasma samples were acquired at 13 time points for 8 h after administration. The concentrations of cephradine in human plasma were measured by a high-performance liquid chromatography (HPLC). Isocratic mobile phase which consisted of acetonitrile, methanol, and 20 mM potassium phosphate (15/5/80, v/v/v, pH 3.48) was used to separate the analytical column cosmosil cholester (250 × 4.6 mm, 3 μm). Analytes were detected in ultraviolet (260 nm). The novel analytical method was described as simple sample preparation, a short retention time (less than 6 min) and making it suitable for use in clinical trials. Pharmacokinetic parameters, such as AUC0-t (20.54 vs 18.42 μg·h/mL), AUC0-infinity (21.22 vs 19.14 μg·h/mL), Cmax (12.69 vs 12.81 μg/mL), Tmax (1.22 vs 0.92 h), half-life (1.02 vs 1.13 h), extrapolation (3.22% vs 3.75%), and Ke (0.73 vs 0.69 h1) were determined for the reference and test drugs in plasma. Pharmacokinetic parameters with a 90% confidence interval were 87% -
The TGF-β1 dynamics during radiation therapy and its correlation to symptomatic radiation pneumonitis in lung cancer patients
Kim Ji-Yoon,Kim Yeon-Sil,Kim Young-Kyoon,Park Hyun-Jin
Radiation Oncology , 2009,
Abstract: Backgroud The underlying molecular and cellular mechanisms of radiation pneumonitis (RP) are very complex. Several biological factors need to be considered together with the well known dosimetric parameters for understanding the molecular events in developing RP in lung cancer patients. The aim of this study was to correlate the variations of the cytokine levels in lung cancer patients during radiation therapy (RT) with the occurrence of symptomatic RP. Methods Thirty-four lung cancer patients who received three-dimensional conformal radiation therapy were evaluated prospectively. Serial blood samples before, at the beginning, in the middle of, at the end of RT and 2 and 4 weeks after RT were analyzed for IL-1α, IL-6, IL-10, TNF-α and TGF-β1 by performing enzyme-linked immunosorbent assay. The predictive values of dosimetric factors for RP were evaluated, too. Results Overall, 8 patients (23.5%) had grade ≥ 2 RP. By serial measurement of cytokines level, only the TGF-β1 level showed a correlation to the symptomatic RP. None of the other cytokines, IL-1α, IL-6, IL-10 and TNF-α level was correlated with the risk of RP. The mean pretreatment TGF-β1 level did not differ between RP and non-RP groups. However, during the period of radiation treatment, the TGF-β1 level began to increase at the end of RT in the RP group and became significantly higher 4 weeks after RT (p = 0.007). Using an ANOVA model for repeated-measures, we found significant associations between the changes of TGF-β1 during the time course of the RT and the risk of developing RP (p < 0.001). Most of the dosimetric factors showed a significant association with RP. Conclusion Our results show that the changes of TGF-β1 could be correlated with RP and the incorporation of the biological parameters into the dosimetric data could be useful for predicting symptomatic RP.
Physical Passaging of Embryoid Bodies Generated from Human Pluripotent Stem Cells
Mi-Young Son,Hyun-jin Kim,Min-Jeong Kim,Yee Sook Cho
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019134
Abstract: Spherical three-dimensional cell aggregates called embryoid bodies (EBs), have been widely used in in vitro differentiation protocols for human pluripotent stem cells including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Recent studies highlight the new devices and techniques for hEB formation and expansion, but are not involved in the passaging or subculture process. Here, we provide evidence that a simple periodic passaging markedly improved hEB culture condition and thus allowed the size-controlled, mass production of human embryoid bodies (hEBs) derived from both hESCs and hiPSCs. hEBs maintained in prolonged suspension culture without passaging (>2 weeks) showed a progressive decrease in the cell growth and proliferation and increase in the apoptosis compared to 7-day-old hEBs. However, when serially passaged in suspension, hEB cell populations were significantly increased in number while maintaining the normal rates of cell proliferation and apoptosis and the differentiation potential. Uniform-sized hEBs produced by manual passaging using a 1:4 split ratio have been successfully maintained for over 20 continuous passages. The passaging culture method of hEBs, which is simple, readily expandable, and reproducible, could be a powerful tool for improving a robust and scalable in vitro differentiation system of human pluripotent stem cells.
Trichostatin A enhances acetylation as well as protein stability of ERα through induction of p300 protein
Sung-Hye Kim, Hyun-Jin Kang, Hyelin Na, Mi-Ock Lee
Breast Cancer Research , 2010, DOI: 10.1186/bcr2562
Abstract: Transient transfection was performed using the Welfect-EX?Plus procedure. The mRNA expression was determined using RT-PCR. Protein expression and interaction were determined by western blotting and immunoprecipitation. The transfection of siRNAs was performed using the Oligofectamine? reagent procedure.TSA treatment increased acetylation of ERα in a dose-dependent manner. The TSA-induced acetylation of ERα was accompanied by an increased stability of ERα protein. Interestingly, TSA also increased the acetylation and the stability of p300 protein. Overexpression of p300 induced acetylation and stability of ERα by blocking ubiquitination. Knockdown of p300 by RNA interference decreased acetylation as well as the protein level of ERα, indicating that p300 mediated the TSA-induced stabilization of ERα.We report that TSA enhanced acetylation as well as the stability of the ERα protein by modulating stability of p300. These results may provide the molecular basis for pharmacological functions of HDAC inhibitors in the treatment of human breast cancer.Estrogen receptors (ERs) are members of a nuclear hormone receptor superfamily. ERs exist in two isoforms, ERα and ERβ, which have highly conserved DNA binding domains and ligand binding domains [1,2]. Although these receptors display similar binding affinities for 17β-estradiol, they have distinct roles in the regulation of gene expression and different interactions with unique sets of transcriptional factors [2]. Activation of ERα is considered a risk factor for the development of breast cancer, since the activation leads to cellular proliferation [3,4]. Cumulative data from tumor biopsies in the clinic have shown that two-thirds of breast cancers are ER-positive [5,6]. Tamoxifen, which regulates ERα activity, reduces the recurrence and death rate of ERα-positive breast cancer [7]. Breast cancer patients with expression of ERα are seven to eight times more likely to benefit from selective estrogen receptor modulators such a
Designing a Global Authentication Infrastructure
Stephanos Matsumoto,Raphael M. Reischuk,Pawel Szalachowski,Tiffany Hyun-Jin Kim,Adrian Perrig
Computer Science , 2015,
Abstract: We address the problem of scaling authentication for naming, routing, and end-entity certification to a global environment in which authentication policies and users' sets of trust roots vary widely. The current mechanisms for authenticating names (DNSSEC), routes (BGPSEC), and end-entity certificates (TLS) do not support a coexistence of authentication policies, affect the entire Internet when compromised, cannot update trust root information efficiently, and do not provide users with the ability to make flexible trust decisions. We propose a Scalable Authentication Infrastructure for Next-generation Trust (SAINT), which partitions the Internet into groups with common, local trust roots, and isolates the effects of a compromised trust root. SAINT requires groups with direct routing connections to cross-sign each other for authentication purposes, allowing diverse authentication policies while keeping all entities globally verifiable. SAINT makes trust root management a central part of the network architecture, enabling trust root updates within seconds and allowing users to make flexible trust decisions. SAINT operates without a significant performance penalty and can be deployed alongside existing infrastructures.
Development of a Novel Recombinant Heamagglutinin-Neuramindase Elisa (rHN-ELISA) for Evaluation of Humoral Immunity in Chicken Vaccinated Against Newcastle Disease Virus (NDV)
Ho-Jin Moon,Jung-Eun Park,Hyeon Yoon,Deu John M. Cruz,Chul-Joong Kim,Hyun-Jin Shin
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2010.2932.2939
Abstract: Best known method to prevent recurrent out breaks of Newcastle Disease (ND) is by vaccination with specific viral antigens along with better managemental practices. Even after many viral strains utilized for vaccine development, sporadic incidence of ND outbreaks are reported every year. In the present study, an attempt was made to develop a modified diagnostic tool i.e., a recombinant ELISA (rELISA) using prokaryotic expressed recombinant NDV HN (Hemagglutinin-Neuraminidase) protein i.e., the recombinant ND viral protein was cloned and expressed in E. coli after confirmation of the expression; purified protein was used as the coating antigen for rHN-ELISA to study the utility of the presently commercially available vaccines and to compare the present diagnostic method with other alternative detection methods such as ELISA and HI which justified its potential use as a novel diagnostic assay for NDV vaccines. Therefore, an attempt was made to utilise the HN glycoproteins which are fundamentally involved in eliciting immune response against NDV, so that evaluation of antibodies against HN proteins would provide further specific assessment of antibody titres when compared to available diagnostic methods which target to detect antibodies against whole virus. Such novel methods are expected in turn in development of more specific vaccines. Though, rHN-ELISA was inconclusive in its results to substantiate by SN (Serum Neutralizing) test for clearness of neutralizing antibody detection, it may envisage a novel approach towards countering ND outbreaks by supporting its diagnosis and prevention.
Ischemic colitis due to obstruction of mesenteric and splenic veins: A case report
Seong-Su Hwang, Woo-Chul Chung, Kang-Moon Lee, Hyun-Jin Kim, Chang-Nyol Paik, Jin-Mo Yang
World Journal of Gastroenterology , 2008,
Abstract: Ischemic injury to the bowel is a well known disease entity that has a wide spectrum of pathological and clinical findings. A sudden drop in the colonic blood supply is essential to its development. We encountered a 41-year-old male patient, who presented with abdominal pain and bloody diarrhea. A colonoscopy showed markedly edematous mucosa with tortuous dilatation of the veins and a deep ulceration at the rectosigmoid junction. On an abdominal computed tomography (CT) scan and CT angiography, the mesenteric and splenic veins were absent with numerous venous collaterals for drainage. The patient gradually responded to oral aminosalicylate therapy, and was in remission after nine months. In most cases, non-occlusive ischemic injury is caused by idiopathic form and occlusive ischemia is caused by abnormalities of arteries and acute venous thrombosis. However, chronic venous insufficiency due to obstruction of macrovascular mesenteric vein rarely causes ischemia of the bowel. This report describes the first case of ischemic colitis caused by obstruction of the mesenteric and splenic veins.
A Practical System for Guaranteed Access in the Presence of DDoS Attacks and Flash Crowds
Yi-Hsuan Kung,Taeho Lee,Po-Ning Tseng,Hsu-Chun Hsiao,Tiffany Hyun-Jin Kim,Soo Bum Lee,Yue-Hsun Lin,Adrian Perrig
Computer Science , 2015,
Abstract: With the growing incidents of flash crowds and sophisticated DDoS attacks mimicking benign traffic, it becomes challenging to protect Internet-based services solely by differentiating attack traffic from legitimate traffic. While fair-sharing schemes are commonly suggested as a defense when differentiation is difficult, they alone may suffer from highly variable or even unbounded waiting times. We propose RainCheck Filter (RCF), a lightweight primitive that guarantees bounded waiting time for clients despite server flooding without keeping per-client state on the server. RCF achieves strong waiting time guarantees by prioritizing clients based on how long the clients have waited-as if the server maintained a queue in which the clients lined up waiting for service. To avoid keeping state for every incoming client request, the server sends to the client a raincheck, a timestamped cryptographic token that not only informs the client to retry later but also serves as a proof of the client's priority level within the virtual queue. We prove that every client complying with RCF can access the server in bounded time, even under a flash crowd incident or a DDoS attack. Our large-scale simulations confirm that RCF provides a small and predictable maximum waiting time while existing schemes cannot. To demonstrate its deployability, we implement RCF as a Python module such that web developers can protect a critical server resource by adding only three lines of code.
Impact of acute exercise on brachial artery flow-mediated dilatation in young healthy people
In-Chang Hwang, Kyung-Hee Kim, Won-Suk Choi, Hyun-Jin Kim, Moon-Sun Im, Yong-Jin Kim, Sang-Hyun Kim, Myung-A Kim, Dae-Won Sohn, Joo-Hee Zo
Cardiovascular Ultrasound , 2012, DOI: 10.1186/1476-7120-10-39
Abstract: Eighty-six young healthy volunteers were prospectively enrolled from January 2011 to December 2011. The subjects completed FMD tests at rest and immediately after treadmill exercise test. Primary outcome was the impact of acute exercise on FMD, measured by the difference of FMD before and after exercise. Secondary outcomes were the relationship of gender and exercise habit with FMD.Seventy-four subjects who met the eligibility criteria were included for analysis. Thirty-five (47.3%) were male, and the mean age was 22.7±2.7 years. FMD was reduced after exercise (8.98±4.69 to 7.51±4.03%; P=0.017) and the reduction was found in female group (10.36±5.26 to 7.62±3.71%; P=0.002) but not in male group. Post-exercise FMD was significantly impaired in subjects who did not exercise regularly (6.92±3.13% versus 8.95±5.33%; P=0.003). The decrease of FMD after exercise was greater in female group (?2.75±5.28% versus 0.27±3.24%; P=0.003) and was associated with exercise habit (β=2.532; P=0.027).In healthy young subjects, FMD was reduced after a bout of acute exercise. The impact of acute exercise showed significant differences according to gender and exercise habit. FMD impairment after acute exercise was observed in females and subjects without regular exercise.Endothelial dysfunction is an important factor in the development of atherosclerosis, hypertension, and coronary artery disease (CAD) [1-4]. Impaired endothelial function is observed in subjects with risk factors for atherosclerosis and in patients with essential hypertension [2,5,6]. Also it is an important predictor of long-term cardiovascular events [7,8].There are several methods to assess endothelial dysfunction and flow-mediated dilatation (FMD) is the most widely used non-invasive method to assess the endothelial function, reflecting the endothelial nitric oxide (NO) dependent vasodilation in response to occlusion-induced reactive hyperemia [5,9]. As FMD is non-invasive and reliable, allows repeated measurements [1
Pepper EST database: comprehensive in silico tool for analyzing the chili pepper (Capsicum annuum) transcriptome
Hyun-Jin Kim, Kwang-Hyun Baek, Seung-Won Lee, JungEun Kim, Bong-Woo Lee, Hye-Sun Cho, Woo Taek Kim, Doil Choi, Cheol-Goo Hur
BMC Plant Biology , 2008, DOI: 10.1186/1471-2229-8-101
Abstract: We built the Pepper EST database to mine the complexity of chili pepper ESTs. The database was built on 122,582 sequenced ESTs and 116,412 refined ESTs from 21 pepper EST libraries. The ESTs were clustered and assembled into virtual consensus cDNAs and the cDNAs were assigned to metabolic pathway, Gene Ontology (GO), and MIPS Functional Catalogue (FunCat). The Pepper EST database is designed to provide a workbench for (i) identifying unigenes in pepper plants, (ii) analyzing expression patterns in different developmental tissues and under conditions of stress, and (iii) comparing the ESTs with those of other members of the Solanaceae family. The Pepper EST database is freely available at http://genepool.kribb.re.kr/pepper/ webcite.The Pepper EST database is expected to provide a high-quality resource, which will contribute to gaining a systemic understanding of plant diseases and facilitate genetics-based population studies. The database is also expected to contribute to analysis of gene synteny as part of the chili pepper sequencing project by mapping ESTs to the genome.Pepper is a member of the family Solanaceae, which is one of the largest families in the plant kingdom and includes more than 3,000 species [1]. The Solanaceae family includes important crops, such as pepper, tomato, tobacco, potato, and eggplant and has been highly cultivated over the years for human nutrition and health. Capsicum species are consumed worldwide and are valued because of their unique color, pungency, and aroma. Capsicum peppers include C. annuum, C. chinense, C. baccatum, C. frutescens, and C. pubescens and are cultivated in different parts of the world. Of these, the varieties of the chili pepper plant species C. annuum, having a modest-sized diploid genome (2n = 24), are the most heavily consumed due to their nutritional value and spicy taste [2]. The chemical that is primarily responsible for the pungency of C. annuum has been identified as capsaicin [3], which elicits numerous b
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