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Search Results: 1 - 10 of 1158 matches for " Huma Anwar "
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Molecular Detection and Sequencing for S1 Glycoprotein Gene of Bronchitis Virus of 2016 Epidemic from Sindh and Punjab  [PDF]
Ahmad Umer Sultan, Muhammad Danish Mehmood, Rameez Hassan, Huma Anwar, Sana Noreen, Faisal Amin, Sajjad Hussain
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.912044
Abstract: Infectious Bronchitis (IB) is highly contagious disease of commercial poultry causing substantial economic loses by producing poor quality meat in broilers and effecting production in breeder birds. The causative agent has been reported as most hazardous pathogen among other infectious agent even after being immunized with multi-variant strain vaccine. Currently, different strain such as H-120, 4/91 and D274 have been used extensively for immunoprophylaxis against velogenic strain across Pakistan with minimal protection reported. In current study PCR analysis was used to investigate the molecular nature of IB isolates from Punjab and Sind province of Pakistan in 2016 epidemics. Total of 100 tracheal samples were considered for virus inoculation in 10 days old chicken embryonated eggs. The IBV infected amniotic fluid was neutralized with monoclonal antisera of H-120, 4/91 and D274 strains. The IBV screened samples were subjected for RNA extraction and subsequent to PCR using type specific primer of each strain. The amplified product of 840 bp was sequenced through Sanger sequencing. On the basis of PCR results, four similar amplified products from both regions were obtained showing similarities in agarose gel electrophoresis, but they differ from each other on the basis of nucleotides sequence. Phylogenetic analysis revealed that nucleotide sequences of isolates from Karachi were similar to the IBV H-120, Mass-41 and Connecticut 46 reference strains. Whereas, isolates from the Punjab province are analogous to the Mans-2, Mans-3, 9/41(UK) but did not show significant similarity with other reference strain. Therefore, it is recommended that use of M-41 and H-120 in vaccine production could be effective measure against velogenic infectious agent in Sindh particularly in Karachi, whereas, it would be better to incorporate either of the variant GQ281656.1, AY279533.1 in vaccine because of their highest level of resemblance with genetically sequenced isolates from Lahore and its surroundings.
Immunomodulatory Effect of Purified Exotoxins of Staphylococcus aureus in Association with Bird Flu Virus Vaccine in Broilers  [PDF]
Muhammad Usman Ghani, Muhammad Danish Mehmood, Amna Javed, Farheen Ansari, Huma Anwar, Sana Noreen, Sajjad Hussain Shah
American Journal of Molecular Biology (AJMB) , 2019, DOI: 10.4236/ajmb.2019.91001
Abstract: Immunization is the most effective method still used against infectious agents. Although not always, vaccines ineffectiveness is reported enormously against many of the pathogens throughout the world in poultry, particularly in case of killed or sub unit vaccine. The current project is, therefore, carried out as a preliminary study on broiler chickens to investigate the modulation of immune system against avian influenza virus in association with purified Staphylococcus aureus toxoid. After isolation of Gram positive cocci bacteria on mannitol salt agar from raw milk, yogurt and chicken meat were subsequently biochemically characterized by using rapid diagnostic kit. Pure culture of S. aureus was inoculated into digitally controlled bio-fermentor containing mannitol salt broth for production of toxins. Enormous production of bacteria was passed through sequence of filtration system based on 0.45 μm followed by 0.22 μm size. The centrifugation of the filtrate was made at 10,000 rpm for 60 minutes at 5 followed by 56,100 rpm for 20 minutes and clear supernatant containing Staphylococcus enterotoxin (SEs) was obtained. Bradford estimation of proteins further provided 305 μg/ml of SEs toxoid. Four types of oil adjuvant avian influenza type H9N2 virus vaccines (without toxoid, 91.5 μg/0.3ml, 22.8 μg/0.3ml and 11.43 μg/0.3ml) were injected into healthy AI H9N2 susceptible broilers and anti-H9N2 HI antibody titers were measured in terms of hemagglutination inhibition test. It was observed that on the 8th day
The association of complex liver disorders with HBV genotypes prevalent in Pakistan
Saeeda Baig, Anwar Siddiqui, Waqaruddin Ahmed, Huma Qureshi, Ambreen Arif
Virology Journal , 2007, DOI: 10.1186/1743-422x-4-128
Abstract: A total of 295 patients with HBsAg positive were selected from the Pakistan Medical Research Council's (PMRC) out patient clinics. Two hundred and twenty six (77%) were males, sixty nine (23%) were females (M to F ratio 3.3:1).Out of 295 patients, 156 (53.2%) had Acute(CAH), 71 (24.2%) were HBV Carriers, 54 (18.4%) had Chronic liver disease (CLD) Hepatitis. 14 (4.7%) were Cirrhosis and HCC patients. Genotype D was the most prevalent genotype in all categories of HBV patients, Acute (108), Chronic (39), and Carrier (53).Cirrhosis/HCC (7) were HBV/D positive. Genotype A was the second most prevalent with 28 (13%) in acute cases, 12 (22.2%) in chronics, 14 (19.7%) in carriers and 5 (41.7) in Cirrhosis/HCC patients. Mixed genotype (A/D) was found in 20 (12.8%) of Acute patients, 3 (5.6%) of Chronic and 4 (5.6%) of carriers, none in case of severe liver conditions.Mixed HBV genotypes A, D and A/D combination were present in all categories of patients except that no A/D combination was detected in severe conditions. Genotype D was the dominant genotype. However, genotype A was found to be more strongly associated with severe liver disease. Mixed genotype (A/D) did not significantly appear to influence the clinical outcome.HBV is a classical virus that has amazed the researchers and clinicians around the world first with geographic relationship of its genotypes then secondly the association of its different genotypes with a wide spectrum of clinical manifestations. In the recent years, there has been an explosion of knowledge regarding clinical significance of HBV genotypes in terms of clinical outcomes and therapeutic response to antiviral therapy in patients with HBV related severe liver conditions [1]. Approximately 2 billion people in the world are infected by HBV [2], More than 350 million people are chronic carriers of the virus [3] Acute hepatitis of varying severity exists in 95% of children and 2–10 % of adult patients [4]. Overall, less than 1 % of acute infectio
Comparison of Shisha Smoking Behavior among Medical and Pharmacy Students  [PDF]
Ashok Kumar, Salman Baig, Sana Ansari, Nadeem Rizvi, Huma Sharif, Anwar Ejaz Beg, Ammara Rauf, Faraz Ahmed Baig, Ammara Abdul Majeed
Journal of Behavioral and Brain Science (JBBS) , 2016, DOI: 10.4236/jbbs.2016.67027
Abstract: Background: Health care workers are respected in every society and have a strong influence on the behaviour of the community. In order to reduce the shisha dependence in the Pakistani population, it is necessary to evaluate the perspective of future doctors and pharmacists regarding this addiction as they become role models for the future generations. Objective: The aim is to compare the knowledge, attitudes, and practices of shisha use among medical students and pharmacy students in order to assess their support in the anti-shisha campaigns of Pakistan. Methodology: Cross sectional study was performed in four major medical universities of Karachi, Pakistan through a modification of the WHO global health survey questionnaire. Result: 398 medical students and 470 pharmacy students were included in the study which showed that currently 9.6% pharmacy students and 4.8% medical students used shisha daily (p = 0.000). Pharmacy students started shisha use in less than 15 years of age while this number was 29.5% among medical students (P = 0.000). Moreover, 44.6% pharmacy students and 50% medical students were not planning to quit this addiction within the next six months. 14% of pharmacy students and 20.4% in medical students (P = 0.002) were unaware that passive smoking caused lung cancer, while 23.4% pharmacy students and 33.5% medical students rejected it as cause of heart diseases (p = 0.006). Conclusion: A significant number of medical and pharmacy students were unaware about some of the serious consequences of shisha smoking. Therefore, both groups of students need to be educated about the health hazards of shisha use, in order to control this growing menace.
Antioxidant Potential Some Medicinal Plants of Central India  [PDF]
Savita Dixit, Huma Ali
Journal of Cancer Therapy (JCT) , 2010, DOI: 10.4236/jct.2010.12014
Abstract: Cellular damage or oxidative injury arising from free radicals or reactive oxygen species (ROS) now appears the fun-damental mechanism underlying a number of human neurodegenerative disorder, diabetes, inflammation, viral infec-tions, autoimmune pathologies and digestive system disorders. Free radicals are generated through normal metabolism of drugs, environmental chemicals and other xenobiotics as well as endogenous chemicals, especially stress hormones (adrenalin and noradrenalin). Accumulated evidence suggests that ROS can be scavenged through chemoprevention utilizing natural antioxidant compounds present in foods and medicinal plants. In this review, research on the antioxi-dant potential of some medicinal plants of origin of Central India is considered.4CdBw3
Experimental Study of Surface and Solution Properties of Gemini -conventional Surfactant Mixtures on Solubilization of Polycyclic Aromatic Hydrocarbon  [PDF]
M. Kamil, Huma Siddiqui
Modeling and Numerical Simulation of Material Science (MNSMS) , 2013, DOI: 10.4236/mnsms.2013.34B004
Abstract:

Experimental data are presented on the enhanced solubilities of fluorene (FLR) resulting from solubilization in aqueous solutions of two conventional surfactants: cationic cetyltrimethylammonium bromide (CTAB) , anionic sodium dodecyl sulfate (SDS), nonioinic polyethylene glycol dodecyl ether (Brij35) and a cationic gemini bis (hexadecyldimethylammonium) pentane dibromide (G5). The critical micellar concentration of surfactants was determined by surface tension measurements and aqueous solubilities of fluorene compound in surfactant solutions were measured spectrophotometrically. Solubilization of PAH compound commenced at the surfactant critical micelle concentration and was proportional to the concentration of surfactant in micelle. The results of the mixed systems were analyzed with the help of regular solution theory, in which the deviation of CMCexp values for mixed surfactant systems from CMCideal was measured by evaluating the interaction parameter, βm. Negative values of βm were observed in all equimolar binary systems which show synergism in the mixed micelle. Attraction force between two oppositely charged head groups lead the strongest synergism effect between cationic gemini and anionic conventional surfactant. In addition to molar solubilization ratio (MSR) solubilization efficiency is also quantified in terms of micelle-water partition coefficient (Km).

Le Fondement de Principe du Système Institutionnel Communautaire
Ioan Huma
Acta Universitatis Danubius : Relationes Internationales , 2010,
Abstract: The system of EU institutions is the visible part of the complex interrelationships in whichfunctional expression - resulting in - the authority delegated by the member states and peoples of theregion, European Community, Communities and the EU. In this perspective, EU institutions are notcompletely independent in their reports, as so often it says. Sharing of their duties shows the contrary,the interdependence of institutions. They define themselves by their condition and arrest reportsattributed competence, not by an atomistic status. Their status is one of assigned relationship,function, role.
Principes de l’interprétation Juridique
Ioan Huma
EIRP Proceedings , 2009,
Abstract: This paper distinguishes between the general principles of law and the principles ofjudicial interpretation, approaching the statute of the later ones. It is pointed out that, the principlesof the judicial interpretation, although immanently having an axiological foundation ensured by thegeneral principles of law, operate with another register, being functionally formed and orienting theinterpretation towards the context from the technical and methodological point of view, in the sametime having an applicative pattern.
Trousseau’s sign  [PDF]
Anwar Alam
Case Reports in Clinical Medicine (CRCM) , 2013, DOI: 10.4236/crcm.2013.25087
Abstract: The trousseau’s sign is carpopedal spasm that results from ischemia that is induced by pressure applied to the upper arm. It is a sensitive and specific sign of hypocalcemia. Here the author is sending the short case summary, images of the demonstration of the Trousseau’s sign. This case also had a cluster of seizures induced by hypocalcemia.
Practical Techniques for Restoration of Architectural Formation Elements in Historical Buildings  [PDF]
Mahran Anwar
World Journal of Engineering and Technology (WJET) , 2019, DOI: 10.4236/wjet.2019.71013
Abstract: Restoration of architectural formation elements is basically trying in estimating the optimization method which places it old architectural artist in his work. The article aims at rooting the methodology of restoration of the architectural formation elements. It is essentially intellectual effort by the restorer in assessing the best way the artist and the old architect in his work. In order to reach the purpose behind this works and analyze the original available techniques in the creation period, the restoration process can be completed as fully as possible. The research is concerned with re-confidence in the classical methods in the restoration of buildings as consolidation and reconstruction. The classical method is a real way to restore the building to its original form especially in the availability of all technological tools to achieve reporting and documentation. It is not necessary to extension in using unapplied materials and technique. In this regard, the research was able to provide solutions, ideas and techniques that helped preserve and restore elements of architectural formation. The research also pointed to the importance of linking the physical and plastic aspects of the archaeological element.
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