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Search Results: 1 - 10 of 80879 matches for " Huixin;Liu "
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FOCAL LENGTH AND DISTORTION OF 180/185 LOW LIGHT CALIBRATING AND TRACKING TV OPTICAL SYSTEM DETERMINED BY DKM3 -THEODOLITE
用DKM_3经纬仪测量180/185微光标校跟踪电视光学系统的焦距和畸变

LIU HUIXIN,
刘惠欣

光子学报 , 1989,
Abstract: The fine optical instruments used for aerial photographic survey, geodetic survey and celestial body survey and so on must be calibrated and checked completely before leavinng the factory, so as to determine their focal lengths and distortions, and give out corrected values of distortions in permissible range. The paper deals with focal length and distortion of TV optical system determ ined by the high-precision theodolite, and analyzes measuring accuracy, and it puts forward a few principal problems to be noticed.
The Design and Implementation of Qt-based Cross-platform Video Conferencing Remote Control  [PDF]
Hua Yuan, Huixin Du
Communications and Network (CN) , 2013, DOI: 10.4236/cn.2013.51B017
Abstract: In order to overcome the defects of the dedicated video conference remote control, this paper designs and implements a Qt-based cross-platform video conferencing remote control. The remote control not only implements a full range of video conferencing control functions with an easy-to-use visual interface; but also implement cross-platform feature to allow users to use remote control software on their own equipment. It is economic, convenient and stylish for users. This paper mainly describes the design and implementation of remote control system framework and cross-platform features. Finally, the result in the paper shows: This remote control is reliable and easy to use.
Expression of human transforming growth factor β1 inE. coli (I)
Jianyin Long,Huixin Wang,Li Liu,Fang Wang,Tingchong Zhou
Science China Life Sciences , 1998, DOI: 10.1007/BF02882894
Abstract: PCR technique is used to amplify the mature peptide gene of human transforming growth factor pl (hTGFβ1); the gene is verified by full-length sequence analysis. In DHSa/pBV220 expression system, hTGFβ1 attains expression in the cytoplasm ofE. coli up to 16%. The recombinant protein is proved to be the monomer of hTGFPl by N-terminal amino acids analysis and immunoblotting. After refolding of the monomer proteinin vitm in glutathione system or CHPAS/DMSO system, the dimeric protein accumulates to 30% in the refolding mixture. The recombinant protein is purified to homogeneity on silver staining, and is shown to have strong biological activity from MTT bioassay on MvlLu cells.
urce, biological community and soil functional stability dynamics at the soil–litter interface
Manqiang Liu,Xiaoyun Chen,Shi Chen,Huixin Li,Feng Hu
生态学报 , 2011,
Abstract: The interface between decaying plant residues and soil is a focus for soil ecological processes because of resources from the residues diffusing into the soil, and microfauna that proliferate in the adjacent soil. Given that the recovery of soil function following disturbance depends on immigration, colonization and establishment of exotic organisms from adjacent un-disturbed habitats, and the availability of bio-available resources, we hypothesized that the soil–litter interface could contribute to soil functional stability. In laboratory pot trials, soil was separated into two parts by a mesh bag with the inner section amended, or not amended, with rice straw; an outer layer of unamended soil, adjacent to the litter (1.5 cm thick, either heated or not), provided a soil–litter interface. This enabled us to examine the dynamics of dissolved organic carbon (DOC), mineral nitrogen, microbial biomass carbon (MBC), nematode assemblages and functional stability during 35 days incubation. Either 1 mm or 5 μm meshes were used, which allowed nematodes to migrate (SR1) or not (SR5) through the mesh to the soil–litter interface; thus also enabling us to evaluate the role of nematodes in soil functional stability. Higher DOC and MBC but lower mineral nitrogen concentrations were found at the soil–litter interface. Heating increased the availability of soil resources such as mineral nitrogen and DOC, but decreased the MBC and total nematode abundance in the soil. The soil–litter interface was characterized by a higher abundance of nematodes, particularly microbivores, regardless of mesh aperture or disturbance. The difference in nematode abundance between SR1 and SR5 indicated that nematode propagation, due to resource diffusion and nematode migration through the mesh, contributed to the changing numbers of microbivorous nematodes depending on incubation time. The soil functional stability was calculated as a relative change in the functioning of short-term barley decomposition. Soil functional resistance, defined as the instantaneous effect of disturbance on decomposition measured on the first day, was highest in the SR5 treatment. However, soil functional resilience, defined as the recovery of soil function over the whole incubation period (35d), was highest in the SR1 treatment, which is most probably attributed to the functioning of microbivorous nematodes. Our results suggest that small-scale spatial heterogeneity, due to organic residue decomposition, can help maintain soil functions following disturbance.
Different linkages in the long and short regions of the genomes of duck enteritis virus Clone-03 and VAC Strains
Xiaoli Liu, Zongxi Han, Yuhao Shao, Yang Li, Huixin Li, Xiangang Kong, Shengwang Liu
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-200
Abstract: A 42,897-bp fragment located downstream of the LOFR11 gene was amplified from the Clone-03 strain of DEV by using 'targeted gene walking PCR'. Twenty-two open reading frames (ORFs) were predicted and determined in the following order: 5'-LORF11-RLORF1-ORF1-ICP4-S1-S2-US1-US10-SORF3-US2-MDV091.5-like-US3-US4-US5-US6-US7-US8-ORFx-US1-S2-S1-ICP4 -3'. This was different from that of the published VAC strain, both in the linkage of the L region and S region, and in the length of the US10 and US7 proteins. The MDV091.5-like gene, ORFx gene, S1 gene and S2 gene were first observed in the DEV genome. The lengths of DEV US10 and US7 were determined to be 311 and 371 amino acids, respectively, in the Clone-03 strain of DEV, and these were different from those of other strains. The comparison of genomic organization in the fragment studied herein with those of other herpesviruses showed that DEV possesses some unique characteristics, such as the duplicated US1 at each end of the US region, and the US5, which showed no homology with those of other herpesviruses. In addition, the results of phylogenetic analysis of ORFs in the represented fragment indicated that DEV is closest to its counterparts VZV (Varicellovirus) and other avian herpesviruses.The molecular characteristics of the 42,897-bp fragment of Clone-03 have been found to be different from those of the VAC strain. The phylogenetic analysis of genes in this region showed that DEV should be a separate member of the subfamily Alphaherpesvirinae.Herpesviruses are among the most persistent of all pathogens because they have coevolved with their hosts over a long period of time, and they are relatively harmless in immunocompetent hosts [1]. The family Herpesviridae comprises approximately 100 members; these viruses infect a range of host species from humans and other mammals to birds, amphibians, and reptiles [2]. On the basis of differences in cellular tropism, genome organization, and gene content, herpesviruses have been
Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus
Xiaoli Liu, Zongxi Han, Yuhao Shao, Dan Yu, Huixin Li, Yu Wang, Xiangang Kong, Shengwang Liu
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-223
Abstract: A mAb (designated 1C8) was generated against the DEV UL26c protein, and a series of 17 partially overlapping fragments that spanned the DEV UL26c were expressed with GST tags. These peptides were subjected to enzyme-linked immunosorbent assay (ELISA) and western blotting analysis using mAb 1C8 to identify the epitope. A linear motif, 520IYYPGE525, which was located at the C-terminus of the DEV UL26 and UL26.5 proteins, was identified by mAb 1C8. The result of the ELISA showed that this epitope could be recognized by DEV-positive serum from mice. The 520IYYPGE525 motif was the minimal requirement for reactivity, as demonstrated by analysis of the reactivity of 1C8 with several truncated peptides derived from the motif. Alignment and comparison of the 1C8-defined epitope sequence with those of other alphaherpesviruses indicated that the motif 521YYPGE525 in the epitope sequence was conserved among the alphaherpesviruses.A mAb, 1C8, was generated against DEV UL26c and the epitope-defined minimal sequence obtained using mAb 1C8 was 520IYYPGE525. The mAb and the identified epitope may be useful for further study of the design of diagnostic reagents for DEV.Herpesviruses exist widely in nature. The genomes of herpesviruses consist of linear double-stranded DNA; they differ in size (from approximately 124 to 235 kb), sequence arrangement and base composition [1], and vary significantly with respect to the presence and arrangement of inverted and directly repeated sequences. The genomes of most of the alphaherpesviruses, such as herpes simplex virus 2 (HSV-2) [2] and Marek's disease virus 1 (MDV-1) [3], encode more than 70 proteins; some of these proteins are not essential for the replication of the viruses. Only limited information is available about the structures and functions of these 70 proteins, although some studies of the antigenic determinants of the glycoproteins have been reported [4,5]. Three types of capsid, named A-, B-, and C-capsids, are needed in the assemb
Comparative analysis of the genes UL1 through UL7 of the duck enteritis virus and other herpesviruses of the subfamily Alphaherpesvirinae
Li, Huixin;Liu, Shengwang;Han, Zongxi;Shao, Yuhao;Chen, Shuhong;Kong, Xiangang;
Genetics and Molecular Biology , 2009, DOI: 10.1590/S1415-47572009005000003
Abstract: keywords : duck enteritis virus; ul1-ul7 genes; phylogenetic analysis.
Expression of human transforming growth factor β1 in E. coli (I) --Direct expression in cytoplasm

LONG Jianyin,WANG Huixin,LIU Li,WANG Fang,ZHOU Tingchong,

中国科学C辑(英文版) , 1998,
Abstract: PCR technique is used to amplify the mature peptide gene of human transforming growth factor pl (hTGFβ1); the gene is verified by full-length sequence analysis. In DHSa/pBV220 expression system, hTGFβ1 attains expression in the cytoplasm ofE. coli up to 16%. The recombinant protein is proved to be the monomer of hTGFPl by N-terminal amino acids analysis and immunoblotting. After refolding of the monomer proteinin vitm in glutathione system or CHPAS/DMSO system, the dimeric protein accumulates to 30% in the refolding mixture. The recombinant protein is purified to homogeneity on silver staining, and is shown to have strong biological activity from MTT bioassay on MvlLu cells. Project supported by the National Natural Science Foundation of China (Grant No. 39580015)
Prevalence of Unprotected Anal Intercourse among Men Who Have Sex with Men in China: An Updated Meta-Analysis
Jing Wu, Yifei Hu, Yujiang Jia, Yingying Su, Huixia Cui, Huixin Liu, Ning Wang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0098366
Abstract: Objective The purpose of this review was to assess the prevalence of unprotected anal intercourse (UAI) among men who have sex with men (MSM) in China. Methods A comprehensive search was conducted including online databases like “Wanfang”, Chinese National Knowledge Infrastructure, PubMed and manual searches. Analyses using random-effects models were performed to estimate the prevalence of UAI among MSM in China. Results Sixty-two articles reporting eighty-two studies were selected. The pooled prevalence rates of UAI with any male partner, with regular male partners, with non-regular male partners, with casual male partners, and with commercial male partners among MSM were 53%(95%CI: 51–56%), 45%(95%CI: 39–51%), 34%(95%CI: 24–45%), 33%(95%CI: 30–36%), 12% (95%CI: 5–26%), respectively. A cumulative meta-analysis found that the pooled UAI prevalence decreased over time. Conclusions Although the prevalence of UAI with male partners among MSM in China presents a decreasing trend over the past decade, the concomitant rise in HIV prevalence and incidence indicates that current prevention intervention efforts are insufficient to effectively contain the spread of HIV. Therefore, the persistently high prevalence of risky sexual behaviors underscores the need for innovative and effective prevention strategies among MSM.
EXTRACTING COSEISMIC DEFORMATION FIELD RELATED TO WENCHUAN Ms8.0 EARTHQUAKE BY USE OF ALOS DInSAR
使用ALOS DInSAR提取汶川地震同震地表形变场

Zhang Huixin,Liu Guoxiang,Zhang Rui,Jia Hongguo,
张慧鑫
,刘国祥,张瑞,贾洪果

大地测量与地球动力学 , 2011,
Abstract: In order to detect surface deformation caused by the Wenchuan Ms8.0 earthquake,the two-pass differential interferometric synthetic aperture radar(DInSAR) method is employed for analyzing the ground displacement due to the main shock by using both the ALOS satellite PALSAR L-band SAR data and GPS data.We computed the coseismic interferogram and the deformation field with spatial resolution of 15 m in an area of about 83 194 km 2.Moreover,the accuracy of deformation values derived interferometrically is evalu...
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