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Search Results: 1 - 10 of 34504 matches for " Huilin Zhou "
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Neural differentiation of allogenic mixed-cultured rat bone mesenchymal stem cells  [PDF]
Jun Zhou, Huilin Yang, Jiannong Cen
Journal of Biomedical Science and Engineering (JBiSE) , 2013, DOI: 10.4236/jbise.2013.64058

Many studies showed that bone mesenchymal stem cells (BMSCs) can transdifferentiate to neural cell in vitro. The purpose of this study was to investigate the mixed-culture of allogenic rat BMSCs in vitro, and to perform neural differentiation potential characterization. Rat BMSCs were isolated and cultured by plastic adherence and density gradient centrifugation respectively, and the 3rd passage cells were harvested and mixed-cultured with same concentration. The second passage cells of the mixed-cultured cells were obtained to perform Wright-Gemsa staining for morphological observation, to identify the surface marker of CD29, CD45, CD90 by flow cytometry and induced to differentiate into neural cell, then performed immunocytochemistry of Nestin, NSE and GFAP. Results showed that after Wright-Giemsa staining, the mixed-cultured cells displayed typical spindle-shape, presented layered and whirlpool-like growth; and the mixed-cultured cells were positive for CD29 and CD90, but negative for CD45 by flow cytometry. After induction, the mixed-cultured cells appeared morphological changes of neuron and glial cell, and were positive expression of Nestin, neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP). The results demonstrated that the mixed-cultured allogenic rat BMSCs can be induced into neural cell such as neuron and glial cell in vitro.

Design of a CPW-Fed Ultra Wide Band Antenna  [PDF]
Shaowen Hu, Yiqiang Wu, Ye Zhang, Huilin Zhou
Open Journal of Antennas and Propagation (OJAPr) , 2013, DOI: 10.4236/ojapr.2013.12005
Abstract: A CPW-fed ultra-wideband antenna was designed. The antenna was etched on a single-layer copper-cladding substrate, of which the material was FR4 with relative permittivity of 4.4, and the magnitude was 40.0 mm × 50.0 mm × 1.6 mm. The parameters of the antenna are simulated and optimized with HFSS. This paper proposes a new trapezoidal CPW-fed UWB antenna that the bandwidth (return loss ≤ ?10 dB) covers 2.7 - 9.3 GHz range, which means a relative bandwidth of 110% with good radiation patterns and gain. Simulated and measured results for return loss, radiation pattern and gain were presented. A good agreement has been obtained between the simulation and experiment and the proposed antenna meets the requirements of the ultra-wideband antenna.
The Moderating Effects of Item Order Arranged by Difficulty on the Relationship between Test Anxiety and Test Performance  [PDF]
Huilin Chen
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.33052
Abstract: Taking cultural knowledge tests as the case study, this research carries out a series of empirical investigations to verify the moderating effects of item order arranged by difficulty on the relationship between test anxiety and test performance. Groups classified according to test anxiety take tests with two major types of item order: item order arranged according to item bank calibrated item difficulty and item order adjusted according to individual examinee’s perceived item difficulty. The means of those test results are compared between groups to see whether the differences are significant. The investigations obtain the following findings: the higher the test taker’s level of test anxiety, the higher significance of the moderating effects and vice versa; item order adjusted according to individual examinee’s perceived item difficulty may have a more significant moderating effect than item order arranged according to item bank calibrated item difficulty has.
Preserving Yeast Genetic Heritage through DNA Damage Checkpoint Regulation and Telomere Maintenance
Veronica Baldo,Jason Liang,Guoliang Wang,Huilin Zhou
Biomolecules , 2012, DOI: 10.3390/biom2040505
Abstract: In order to preserve genome integrity, extrinsic or intrinsic DNA damages must be repaired before they accumulate in cells and trigger other mutations and genome rearrangements. Eukaryotic cells are able to respond to different genotoxic stresses as well as to single DNA double strand breaks (DSBs), suggesting highly sensitive and robust mechanisms to detect lesions that trigger a signal transduction cascade which, in turn, controls the DNA damage response (DDR). Furthermore, cells must be able to distinguish natural chromosomal ends from DNA DSBs in order to prevent inappropriate checkpoint activation, DDR and chromosomal rearrangements. Since the original discovery of RAD9, the first DNA damage checkpoint gene identified in Saccharomyces cerevisiae, many genes that have a role in this pathway have been identified, including MRC1, MEC3, RAD24, RAD53, DUN1, MEC1 and TEL1. Extensive studies have established most of the genetic basis of the DNA damage checkpoint and uncovered its different functions in cell cycle regulation, DNA replication and repair, and telomere maintenance. However, major questions concerning the regulation and functions of the DNA damage checkpoint remain to be answered. First, how is the checkpoint activity coupled to DNA replication and repair? Second, how do cells distinguish natural chromosome ends from deleterious DNA DSBs? In this review we will examine primarily studies performed using Saccharomyces cerevisiae as a model system.
Application to Three-Dimensional Canonical Correlation Analysis for Feature Fusion in Image Recognition
Xiaogang Gong,Jiliu Zhou,Huilin Wu,Gang Lei
Journal of Computers , 2011, DOI: 10.4304/jcp.6.11.2427-2433
Abstract: This paper presents a three-dimensional canonical correlation analysis (TCCA) method, and applies it to feature fusion for image recognition. It is an extension of traditional canonical correlation analysis (CCA) and two-dimensional canonical correlation analysis (2DCCA). Considering two views of a three-dimensional data, the TCCA can directly find the relations between them without reshaping the data into matrices or vectors, We stress that TCCA dramatically reduce the computational complexity, compared to the CCA and 2DCCA. To evaluate the algorithm, we are using Gabor wavelet to generate the three-dimensional data, and fusing them at the feature level by TCCA. Some experiments on ORL database and JAFEE database and compared with other methods, the results show that the TCCA not only the computing complexity is lower, the recognition performance is better but also suitable for data fusion.
Modeling and prediction for the acute toxicity of pesticide mixtures to the freshwater luminescent bacterium Vibrio qinghaiensis sp.-Q67

Xuefei Zhou Wenjing Sang Shushen Liu Yalei Zhang Huilin Ge,

环境科学学报(英文版) , 2010,
Abstract: In China, water pollution by pesticide mixtures has constituted a serious environmental problem due to potential toxicity and bioaccumulation. But few pesticide combinations have exactly similar and dissimilar mechanisms of action. For this purpose, in tests with the freshwater luminescent bacterium (Vibrio qinghaiensis sp.-Q67), ten pesticides, including three herbicides and seven insecticides, were selected as test substances. Concentration response analysis was performed for ten individual substances, an...
Distinct SUMO Ligases Cooperate with Esc2 and Slx5 to Suppress Duplication-Mediated Genome Rearrangements
Claudio P. Albuquerque,Guoliang Wang,Nancy S. Lee,Richard D. Kolodner,Christopher D. Putnam,Huilin Zhou
PLOS Genetics , 2013, DOI: 10.1371/journal.pgen.1003670
Abstract: Suppression of duplication-mediated gross chromosomal rearrangements (GCRs) is essential to maintain genome integrity in eukaryotes. Here we report that SUMO ligase Mms21 has a strong role in suppressing GCRs in Saccharomyces cerevisiae, while Siz1 and Siz2 have weaker and partially redundant roles. Understanding the functions of these enzymes has been hampered by a paucity of knowledge of their substrate specificity in vivo. Using a new quantitative SUMO-proteomics technology, we found that Siz1 and Siz2 redundantly control the abundances of most sumoylated substrates, while Mms21 more specifically regulates sumoylation of RNA polymerase-I and the SMC-family proteins. Interestingly, Esc2, a SUMO-like domain-containing protein, specifically promotes the accumulation of sumoylated Mms21-specific substrates and functions with Mms21 to suppress GCRs. On the other hand, the Slx5-Slx8 complex, a SUMO-targeted ubiquitin ligase, suppresses the accumulation of sumoylated Mms21-specific substrates. Thus, distinct SUMO ligases work in concert with Esc2 and Slx5-Slx8 to control substrate specificity and sumoylation homeostasis to prevent GCRs.
The Co-Repressor SMRT Delays DNA Damage-Induced Caspase Activation by Repressing Pro-Apoptotic Genes and Modulating the Dynamics of Checkpoint Kinase 2 Activation
Claudio Scafoglio, Marcus Smolka, Huilin Zhou, Valentina Perissi, Michael G. Rosenfeld
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0059986
Abstract: Checkpoint kinase 2 (Chk2) is a major regulator of DNA damage response and can induce alternative cellular responses: cell cycle arrest and DNA repair or programmed cell death. Here, we report the identification of a new role of Chk2 in transcriptional regulation that also contributes to modulating the balance between survival and apoptosis following DNA damage. We found that Chk2 interacts with members of the NCoR/SMRT transcriptional co-regulator complexes and serves as a functional component of the repressor complex, being required for recruitment of SMRT on the promoter of pro-apoptotic genes upon DNA damage. Thus, the co-repressor SMRT exerts a critical protective action against genotoxic stress-induced caspase activation, repressing a functionally important cohort of pro-apoptotic genes. Amongst them, SMRT is responsible for basal repression of Wip1, a phosphatase that de-phosphorylates and inactivates Chk2, thus affecting a feedback loop responsible for licensing the correct timing of Chk2 activation and the proper execution of the DNA repair process.
Strong Law of Large Numbers of the Offspring Empirical Measure for Markov Chains Indexed by Homogeneous Tree
Huilin Huang
ISRN Applied Mathematics , 2012, DOI: 10.5402/2012/536530
Impact of Parent’s Socioeconomic Status on Perceived Parental Pressure and Test Anxiety among Chinese High School Students
Huilin Chen
International Journal of Psychological Studies , 2012, DOI: 10.5539/ijps.v4n2p235
Abstract: This study carries out empirical researches among Mainland Chinese high school students to explore the impact of parent’s socioeconomic status on perceived parental pressure and test anxiety. The discoveries of the study include: perceived parental pressure has significant impact on test anxiety; parents’ occupations, parents’ income and mother’s education have significant impact on perceived parental pressure; parents’ occupations, parents’ income and mother’s education have significant impact on test anxiety. There are sufficient evidences to support the notion that the ethic stressing family glory and material success can be a major source of perceived parental pressure and test anxiety in China. Another finding of the study is that there may exist a mediation relationship among parent’s socioeconomic status, perceived parental pressure, and test anxiety. By controlling perceived parental pressure, the mediator variable, the impact of parent’s socioeconomic status on test anxiety can be greatly reduced.
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