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Search Results: 1 - 10 of 59019 matches for " Huilin Yang "
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Neural differentiation of allogenic mixed-cultured rat bone mesenchymal stem cells  [PDF]
Jun Zhou, Huilin Yang, Jiannong Cen
Journal of Biomedical Science and Engineering (JBiSE) , 2013, DOI: 10.4236/jbise.2013.64058
Abstract:

Many studies showed that bone mesenchymal stem cells (BMSCs) can transdifferentiate to neural cell in vitro. The purpose of this study was to investigate the mixed-culture of allogenic rat BMSCs in vitro, and to perform neural differentiation potential characterization. Rat BMSCs were isolated and cultured by plastic adherence and density gradient centrifugation respectively, and the 3rd passage cells were harvested and mixed-cultured with same concentration. The second passage cells of the mixed-cultured cells were obtained to perform Wright-Gemsa staining for morphological observation, to identify the surface marker of CD29, CD45, CD90 by flow cytometry and induced to differentiate into neural cell, then performed immunocytochemistry of Nestin, NSE and GFAP. Results showed that after Wright-Giemsa staining, the mixed-cultured cells displayed typical spindle-shape, presented layered and whirlpool-like growth; and the mixed-cultured cells were positive for CD29 and CD90, but negative for CD45 by flow cytometry. After induction, the mixed-cultured cells appeared morphological changes of neuron and glial cell, and were positive expression of Nestin, neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP). The results demonstrated that the mixed-cultured allogenic rat BMSCs can be induced into neural cell such as neuron and glial cell in vitro.

The Moderating Effects of Item Order Arranged by Difficulty on the Relationship between Test Anxiety and Test Performance  [PDF]
Huilin Chen
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.33052
Abstract: Taking cultural knowledge tests as the case study, this research carries out a series of empirical investigations to verify the moderating effects of item order arranged by difficulty on the relationship between test anxiety and test performance. Groups classified according to test anxiety take tests with two major types of item order: item order arranged according to item bank calibrated item difficulty and item order adjusted according to individual examinee’s perceived item difficulty. The means of those test results are compared between groups to see whether the differences are significant. The investigations obtain the following findings: the higher the test taker’s level of test anxiety, the higher significance of the moderating effects and vice versa; item order adjusted according to individual examinee’s perceived item difficulty may have a more significant moderating effect than item order arranged according to item bank calibrated item difficulty has.
The Role of TLR and Chemokine in Wear Particle-Induced Aseptic Loosening
Qiaoli Gu,Qin Shi,Huilin Yang
Journal of Biomedicine and Biotechnology , 2012, DOI: 10.1155/2012/596870
Abstract: Wear particle-induced periprosthetic osteolysis remains the principal cause of aseptic loosening of orthopaedic implants. Monocytes/macrophages phagocytose wear particles and release cytokines that induce inflammatory response. This response promotes osteoclast differentiation and osteolysis. The precise mechanisms by which wear particles are recognized and induce the accumulation of inflammatory cells in the periprosthetic tissue have not been fully elucidated. Recent studies have shown that toll-like receptors (TLRs) contribute to the cellular interaction with wear particles. Wear particles are recognized by monocytes/macrophages through TLRs coupled with the adaptor protein MyD88. After the initial interaction, wear particles induce both local and systemic migration of monocytes/macrophages to the periprosthetic region. The cellular migration is mediated through chemokines including interleukin-8, macrophage chemotactic protein-1, and macrophage inhibitory protein-1 in the periprosthetic tissues. Interfering with chemokine-receptor axis can inhibit cellular migration and inflammatory response. This paper highlights recent advances in TLR, and chemokine participated in the pathogenesis of aseptic loosening. A comprehensive understanding of the recognition and migration mechanism is critical to the development of measures that prevent wear particle-induced aseptic loosening of orthopaedic implants.
The effect of acupuncture on stroke recovery: study protocol for a randomized controlled trial
Liu Huilin,Zhang Dangsheng,Tan Xiuge,Yang Daqing
BMC Complementary and Alternative Medicine , 2012, DOI: 10.1186/1472-6882-12-216
Abstract: Background Stroke is one of the leading causes of death and disability in China. Current treatments for stroke are limited and achieve no optimal effect. Acupuncture is widely used in the treatment of stroke and in improving the quality of life for patients in China. In most previous clinical studies, the effects of acupuncture have been diverse, and few well-designed randomized controlled trials have been conducted to investigate the long-term effect of acupuncture on acute stroke recovery. Method Three hundred and twenty eight subjects with acute cerebral apoplexy will be recruited. The patients will be randomized into two different groups: the intervention group will receive acupuncture treatment together with Western standard treatment for 2 weeks plus the secondary prevention treatment for 22 weeks; the control group will receive only the Western standard treatment for 2 weeks and the secondary prevention treatment for 22 weeks. The primary outcome measures are Barthel Index and the Stroke-Specific Quality Of Life. The secondary outcome measures are the National Institutes of Health Stroke Scale and Modified Rankin Scale. All assessments will be conducted at the baseline and at weeks 4, 12 and 24 of follow-up. Discussion This study will evaluate the effects of acupuncture on the long-term recovery of acute stroke and on improving the quality of life of the patients. The results of this study will help establish optimal integrated therapeutic strategies for patients with stroke. Trial registration Current Controlled Trials ISRCTN29932220
Regional Variations in the Cellular, Biochemical, and Biomechanical Characteristics of Rabbit Annulus Fibrosus
Jun Li, Chen Liu, Qianping Guo, Huilin Yang, Bin Li
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091799
Abstract: Tissue engineering of annulus fibrosus (AF), the essential load-bearing disc component, remains challenging due to the intrinsic heterogeneity of AF tissue. In order to provide a set of characterization data of AF tissue, which serve as the benchmark for constructing tissue engineered AF, we analyzed tissues and cells from various radial zones of AF, i.e., inner AF (iAF), middle AF (mAF), and outer AF (oAF), using a rabbit model. We found that a radial gradient in the cellular, biochemical, and biomechanical characteristics of rabbit AF existed. Specifically, the iAF cells (iAFCs) had the highest expression of collagen-II and aggrecan genes, while oAF cells (oAFCs) had the highest collagen-I gene expression. The contents of DNA, total collagen and collagen-I sequentially increased from iAF, mAF to oAF, while glycosaminoglycan (GAG) and collagen-II levels decreased. The cell traction forces of primary AFCs gradually decreased from iAFCs, mAFCs to oAFCs, being 336.6±155.3, 199.0±158.8, and 123.8±76.1 Pa, respectively. The storage moduli of iAF, mAF, and oAF were 0.032±0.002, 2.121±0.656, and 4.130±0.159 MPa, respectively. These measurements have established a set of reference data for functional evaluation of the efficacy of AF tissue engineering strategies using a convenient and cost-effective rabbit model, the findings of which may be further translated to human research.
Two-dimensional crystallization and preliminary electron crystallographic result of partially purified F0 from porcine mitochondria
Xujia Zhang,Jingchuan Sun,Hui Yang,Huilin Li,Youguo Huang,Fuyu Yang
Chinese Science Bulletin , 2002, DOI: 10.1360/02tb9077
Abstract: After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembrane sector F0 was specifically extracted from the stripped membranes in the presence of detergent CHAPS and partially purified. Two-dimensional crystals were produced by the reconstitution of the partially purified F0 into asolectin and microdialysis. The obtained crystals are able to diffract to 2 nm. The projection map of the negatively stained crystal shows that the crystal has p4212 symmetry, lattice constant, a=b=14.4 nm. A unit cell contains four F0 molecules.
Two-dimensional crystallization and preliminary electron crystallographic result of partially purified F0 from porcine mitochondria

ZHANG Xujia,SUN Jingchuan,YANG Hui,LI Huilin,HUANG Youguo,YANG Fuyu,

科学通报(英文版) , 2002,
Abstract: After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembrane sector F0 was specifically extracted from the stripped membranes in the presence of detergent CHAPS and partially purified. Two-dimensional crystals were produced by the reconstitution of the partially purified F0 into asolectin and microdialysis. The obtained crystals are able to diffract to 2 nm. The projection map of the negatively stained crystal shows that the crystal has p4212 symmetry, lattice constant, a=b=14.4 nm. A unit cell contains four F0 molecules.
Strong Law of Large Numbers of the Offspring Empirical Measure for Markov Chains Indexed by Homogeneous Tree
Huilin Huang
ISRN Applied Mathematics , 2012, DOI: 10.5402/2012/536530
Abstract:
Impact of Parent’s Socioeconomic Status on Perceived Parental Pressure and Test Anxiety among Chinese High School Students
Huilin Chen
International Journal of Psychological Studies , 2012, DOI: 10.5539/ijps.v4n2p235
Abstract: This study carries out empirical researches among Mainland Chinese high school students to explore the impact of parent’s socioeconomic status on perceived parental pressure and test anxiety. The discoveries of the study include: perceived parental pressure has significant impact on test anxiety; parents’ occupations, parents’ income and mother’s education have significant impact on perceived parental pressure; parents’ occupations, parents’ income and mother’s education have significant impact on test anxiety. There are sufficient evidences to support the notion that the ethic stressing family glory and material success can be a major source of perceived parental pressure and test anxiety in China. Another finding of the study is that there may exist a mediation relationship among parent’s socioeconomic status, perceived parental pressure, and test anxiety. By controlling perceived parental pressure, the mediator variable, the impact of parent’s socioeconomic status on test anxiety can be greatly reduced.
Strong Law of Large Numbers for Hidden Markov Chains Indexed by Cayley Trees
Huilin Huang
ISRN Probability and Statistics , 2012, DOI: 10.5402/2012/768657
Abstract:
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