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Search Results: 1 - 10 of 7120 matches for " Hugo Aguirre Armelin "
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GenFlow: generic flow for integration, management and analysis of molecular biology data
Oikawa, Marcio Katsumi;Broinizi, Marcos Eduardo Bolelli;Dermargos, Alexandre;Armelin, Hugo Aguirre;Ferreira, Jo?o Eduardo;
Genetics and Molecular Biology , 2004, DOI: 10.1590/S1415-47572004000400035
Abstract: a large number of dna sequencing projects all over the world have yielded a fantastic amount of data, whose analysis is, currently, a big challenge for computational biology. the limiting step in this task is the integration of large volumes of data stored in highly heterogeneous repositories of genomic and cdna sequences, as well as gene expression results. solving this problem requires automated analytical tools to optimize operations and efficiently generate knowledge. this paper presents an information flow model , called genflow, that can tackle this analytical task.
Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation
Elaine Hatanaka, Alexandre Dermargos, Aparecida Emiko Hirata, Marco Aurélio Ramirez Vinolo, Angelo Rafael Carpinelli, Philip Newsholme, Hugo Aguirre Armelin, Rui Curi
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0058626
Abstract: The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and γ-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47phox phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47phox mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: γ-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and γ-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts.
A Robust Structural PGN Model for Control of Cell-Cycle Progression Stabilized by Negative Feedbacks
Trepode Nestor,Armelin Hugo,Bittner Michael,Barrera Junior
EURASIP Journal on Bioinformatics and Systems Biology , 2007,
Abstract: The cell division cycle comprises a sequence of phenomena controlled by a stable and robust genetic network. We applied a probabilistic genetic network (PGN) to construct a hypothetical model with a dynamical behavior displaying the degree of robustness typical of the biological cell cycle. The structure of our PGN model was inspired in well-established biological facts such as the existence of integrator subsystems, negative and positive feedback loops, and redundant signaling pathways. Our model represents genes interactions as stochastic processes and presents strong robustness in the presence of moderate noise and parameters fluctuations. A recently published deterministic yeast cell-cycle model does not perform as well as our PGN model, even upon moderate noise conditions. In addition, self stimulatory mechanisms can give our PGN model the possibility of having a pacemaker activity similar to the observed in the oscillatory embryonic cell cycle.
Detecting phylogenetic relations out from sparse context trees
Florencia Leonardi,Sergio R. Matioli,Hugo A. Armelin,Antonio Galves
Statistics , 2008,
Abstract: The goal of this paper is to study the similarity between sequences using a distance between the \emph{context} trees associated to the sequences. These trees are defined in the framework of \emph{Sparse Probabilistic Suffix Trees} (SPST), and can be estimated using the SPST algorithm. We implement the Phyl-SPST package to compute the distance between the sparse context trees estimated with the SPST algorithm. The distance takes into account the structure of the trees, and indirectly the transition probabilities. We apply this approach to reconstruct a phylogenetic tree of protein sequences in the globin family of vertebrates. We compare this tree with the one obtained using the well-known PAM distance.
Determina??o da composi??o mineral de subprodutos agroindustriais utilizados na alimenta??o animal, pela técnica de ativa??o neutr?nica
Filho, José Cleto da Silva;Armelin, Maria José Aguirre;Silva, Aliomar Gabriel da;
Pesquisa Agropecuária Brasileira , 1999, DOI: 10.1590/S0100-204X1999000200012
Abstract: this study aimed to analyze some agroindustrial by-products used to feed animals in order to identify the presence of the main minerals. samples of cotton seed meal, rice, canola, soybean and wheat; fish meal, feather meal, meat meal, feather plus viscera meal; rinds of cotton, rice, orange; citrus pulp and tomato residue were collected in different sites of production of the by-products. the method of analysis used was the neutron activation followed by gamma ray spectrometry. the levels of minerals found in all samples were compared with the levels commonly found in forages. the mineral concentration, even those of the toxic elements, as as, cd and hg, was at low level and did not exceed the maximum tolerable levels for domestic animals.
Protective measures against ultrafiltration failure in peritoneal dialysis patients
Aguirre, Anna Rita;Abensur, Hugo;
Clinics , 2011, DOI: 10.1590/S1807-59322011001200023
Abstract: ultrafiltration failure in patients undergoing peritoneal dialysis is a condition with an incidence that increases over time. it is related to increased cardiovascular morbidity and mortality and is a major cause of the abandonment of the treatment technique. because the number of patients undergoing renal replacement therapy is increasing with society aging and because approximately 10% of this population is treated with peritoneal dialysis, this matter is becoming more common in everyday practice for clinicians involved in the care of patients with chronic renal failure. in this review, we summarize the available measures used to prevent and treat ultrafiltration failure and the current state of research in the field, both in the experimental and clinical settings, focusing on the possible clinical applications of recent findings.
Repartición del recurso trófico entre Mullus barbatus y M. surmuletus en el Mar Catalán (Mediterráneo Noroccidental)
Aguirre Hugo,Sánchez Pilar
Ciencias marinas , 2005,
Abstract: Se examinó la composición trófica de Mullus barbatus y M. surmuletus con el fin de evaluar la repartición del recurso entre estas dos especies simpátricas. Para cada especie, los peces se agruparon en intervalos de clase de 1 cm (longitud total = LT). Se realizó el análisis de agrupamiento utilizando el índice de similitud de Bray-Curtis y el método de agrupación de UPGMA. Previamente, para cada intervalo de clase se estandarizó y transformó a doble raíz cuadrada la abundancia de las presas. Con base en la similitud trófica, se definieron seis nuevos grupos: (I) adultos mayores de M. surmuletus (21–31 cm LT), (II) organismos recién asentados de ambas especies (6–8 cm LT), (III) adultos mayores de M. barbatus (19–21 cm LT), (IV) adultos de M. barbatus (12–18 cm LT), (V) juveniles de ambas especies y (VI) adultos de M. surmuletus (12–20, 22 cm LT). El análisis de similitud (ANOSIM) muestra que existen diferencias significativas entre la composición trófica de los grupos sucesivos (P ≤ 0.05). Si bien estas especies simpátricas consumen una amplia gama de presas en común, la proporción relativa de sus componentes muestra diferencias claras entre especies e incluso dentro de cada especie a lo largo de su desarrollo; estas diferencias se tornan más evidentes en los organismos mayores. Los resultados del presente trabajo resaltan la importancia de la repartición trófica como un mecanismo que facilita la coexistencia intra e inter específica de estas dos especies simpátricas.
A Robust Structural PGN Model for Control of Cell-Cycle Progression Stabilized by Negative Feedbacks
Nestor Trepode, Hugo Armelin, Michael Bittner, Junior Barrera, Marco Gubitoso, Ronaldo Hashimoto
EURASIP Journal on Bioinformatics and Systems Biology , 2007, DOI: 10.1155/2007/73109
Abstract: [1234567891011121314151617181920]
Fibroblast Growth Factor 2 Causes G2/M Cell Cycle Arrest in Ras-Driven Tumor Cells through a Src-Dependent Pathway
Jacqueline Salotti, Matheus H. Dias, Marianna M. Koga, Hugo A. Armelin
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0072582
Abstract: We recently reported that paracrine Fibroblast Growth Factor 2 (FGF2) triggers senescence in Ras-driven Y1 and 3T3Ras mouse malignant cell lines. Here, we show that although FGF2 activates mitogenic pathways in these Ras-dependent malignant cells, it can block cell proliferation and cause a G2/M arrest. These cytostatic effects of FGF2 are inhibited by PD173074, an FGF receptor (FGFR) inhibitor. To determine which downstream pathways are induced by FGF2, we tested specific inhibitors targeting mitogen-activated protein kinase (MEK), phosphatidylinositol 3 kinase (PI3K) and protein kinase C (PKC). We show that these classical mitogenic pathways do not mediate the cytostatic activity of FGF2. On the other hand, the inhibition of Src family kinases rescued Ras-dependent malignant cells from the G2/M irreversible arrest induced by FGF2. Taken together, these data indicate a growth factor-sensitive point in G2/M that likely involves FGFR/Ras/Src pathway activation in a MEK, PI3K and PKC independent manner.
ESTIMACIóN DE CALIDAD EN POLíMEROS EMPLEANDO SENSORES VIRTUALES
HERNáNDEZ G.,HUGO; AGUIRRE C.,JAIME;
DYNA , 2005,
Abstract: in this paper, a methodology for the development of a first principles model based soft sensor is proposed for the estimation of quality properties of chemical products. a practical example is presented where a soft sensor is developed to estimate the molecular weight distribution of a polymer during the polyesterification of a carboxylic diacid and a diol (a-a/b-b) in a stirred-tank batch reactor. results obtained in the validation stage show that it is possible to employ a model-based soft sensor as the input element of an automatic quality control system. the estimation error of the soft sensor is comparable to the measurement error of physical sensors currently available, but the former has the advantage of providing on-line and real-time measurements.
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