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Search Results: 1 - 10 of 97869 matches for " Hsin-Liang Chen "
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The Pharmacological Activities of (?)-Anonaine
Hsing-Tan Li,Hui-Ming Wu,Hsin-Liang Chen,Chi-Ming Liu,Chung-Yi Chen
Molecules , 2013, DOI: 10.3390/molecules18078257
Abstract: Several species of Magnoliaceae and Annonaceae are used in Traditional Chinese Medicine. (?)-Anonaine, isolated from several species of Magnoliaceae and Annonaceae, presents antiplasmodial, antibacterial, antifungal, antioxidation, anticancer, antidepression, and vasorelaxant activity. This article provides an overview of the pharmacological functions of (?)-anonaine.
Bio-Functional Constituents from the Stems of Liriodendron tulipifera
Chien-Chih Chiu,Han-Lin Chou,Pei-Fang Wu,Hsin-Liang Chen,Hui-Min Wang,Chung-Yi Chen
Molecules , 2012, DOI: 10.3390/molecules17044357
Abstract: Four known compounds have been isolated from the stems of Liriodendron tulipifera, and the structures of these pure constituents were determined using spectroscopic analysis. Isolated compounds were screened for free radical scavenging ability, metal chelating power assay and ferric reducing antioxidant power assay (FRAP). The anti-tyrosinase effects of L. tulipifera compounds were calculated the inhibition of hydroxylation of L-tyrosine to L-dopa according to an in vitro mushroom tyrosinase assay. The study also examined the bio-effects of the four compounds on the human melanoma A375.S2, and showed that liriodenine (1) and (-)-norglaucine (4) significantly inhibited the proliferation of melanoma cells in the cell viability assay. Wound healing results indicated that liriodenine (1), (-)-glaucine (3) and (-)-norglaucine (4) exerted anti-migration potential. Interestingly, (-)-glaucine (3), neither liriodenine (1) nor (-)-norglaucine (4) showed promising anti-migration potential without inducing significant cytotoxicity. Furthermore, a dramatically increased level of intracellular reactive oxygen species (ROS) was detected from (-)-glaucine (3). The cell cycle assessment demonstrated a moderate G2/M accumulation by (-)-glaucine (3). The above results revealed the anti-cancer effects of L. tulipifera compounds, especially on the anti-migration ability indicating the promising chemopreventive agents to human skin melanoma cells.
A highly efficient β-glucosidase from the buffalo rumen fungus Neocallimastix patriciarum W5
Hsin-Liang Chen, Yo-Chia Chen, Mei-Yeh J Lu, Jui-Jen Chang, Hiaow-Ting C Wang, Tzi-Yuan Wang, Sz-Kai Ruan, Tao-Yuan Wang, Kuo-Yen Hung, Hsing-Yi Cho, Huei-Mien Ke, Wan-Ting Lin, Ming-Che Shih, Wen-Hsiung Li
Biotechnology for Biofuels , 2012, DOI: 10.1186/1754-6834-5-24
Abstract: In this study, a cDNA encoding β-glucosidase was isolated from the buffalo rumen fungus Neocallimastix patriciarum W5 and is named NpaBGS. It has a length of 2,331 bp with an open reading frame coding for a protein of 776 amino acid residues, corresponding to a theoretical molecular mass of 85.1 kDa and isoelectric point of 4.4. Two GH3 catalytic domains were found at the N and C terminals of NpaBGS by sequence analysis. The cDNA was expressed in Pichia pastoris and after protein purification, the enzyme displayed a specific activity of 34.5 U/mg against cellobiose as the substrate. Enzymatic assays showed that NpaBGS was active on short cello-oligosaccharides from various substrates. A weak activity in carboxymethyl cellulose (CMC) digestion indicated that the enzyme might also have the function of an endoglucanase. The optimal activity was detected at 40°C and pH 5?~?6, showing that the enzyme prefers a weak acid condition. Moreover, its activity could be enhanced at 50°C by adding Mg2+ or Mn2+ ions. Interestingly, in simultaneous saccharification and fermentation (SSF) experiments using Saccharomyces cerevisiae BY4741 or Kluyveromyces marxianus KY3 as the fermentation yeast, NpaBGS showed advantages in cell growth, glucose production, and ethanol production over the commercial enzyme Novo 188. Moreover, we showed that the KY3 strain engineered with the NpaNGS gene can utilize 2 % dry napiergrass as the sole carbon source to produce 3.32 mg/ml ethanol when Celluclast 1.5 L was added to the SSF system.Our characterizations of the novel β-glucosidase NpaBGS revealed that it has a preference of weak acidity for optimal yeast fermentation and an optimal temperature of ~40°C. Since NpaBGS performs better than Novo 188 under the living conditions of fermentation yeasts, it has the potential to be a suitable enzyme for SSF.
Sensing of Zinc-Containing Nanopollutants with an Ionic Liquid
Michelle Wang,James Wang,H.-C. Wang,Hsin-Liang Huang,H.-L. Liu
Journal of Nanomaterials , 2010, DOI: 10.1155/2010/309207
Abstract: The rapid maturing of nanotechnology and its wide range of applications not bring benefits only, so the downsides are worth noting. Nanopollutants, generally undetectable, are often found as byproducts involved in various chemical or physical reactions. Many nanopollutants are concerned and suspected of causing negative impact on human health due to their unpredictable existence in the environment. Unlike the traditional sensing systems which can detect select gaseous molecules, effective nanoparticle sensing methods are still lacking in the literature. In this paper, sensing of zinc-containing nanopollutants suspended in N2 with a room-temperature ionic liquid (RTIL) ([C4mim][PF6] (1-butyl-3-methyl imidazolium hexafluorophosphate)) has been studied. The sensitivities ( ) obtained by determination of the RTIL resistance to the absorbed phosphor fly ash (<200?nm) is 7.0, while the RTIL has higher sensitivity of 11 and 10, respectively, to ZnO and ZnS nanoparticles By component-fitted X-ray absorption near edge structure (XANES) spectroscopy, it seems that the N-methyl imidazole (mim) complexes are formed during sensing of the phosphor fly ash, ZnO and ZnS nanoparticles with the RTIL. The 1H and 31P NMR observations also suggest that the (mim) behaves as a carrier during sensing of nanopollutants with the RTIL. 1. Introduction Nanotechnology developments and applications have been growing very rapidly especially in the recent decades. Many nanosize (<100?nm) materials have been widely used in catalysis, photon-electronic devices, and surface treatments. However, the hidden dangers of nanopollutants from the diverse sources may exacerbate the environment and human health on a daily basis [1]. Nanopollutants may diffuse into the deep lung [2]. Unknown diseases caused by nanopollutants existing in the environment have been suspected [1, 2]. Room-temperature ionic liquids (RTILs) are generally nonvolatile, nonflammable, and with high thermal stability at the temperature range of 273–573?K. Therefore, RTILs are also named “Green solvents” [3]. Room-temperature ionic liquids can also be applied in the industrial synthesis, separation, electrochemistry, and catalysis [3–6]. Organic metals (such as Cd, Pb, or Zn dithizonates) could be absorbed in RTILs at a pH value range of 4–8 [7]. Recently, it was found that Au nanoparticles could be well suspended in the RTIL ([C4mim][PF6]) for at least 20 days [8]. Observations referenced above [4–8] implicate the potential applications of the RTIL in sensing of nanopollutants. Although it is well known that the selected
Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses
Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou, Ya-Wen Tsai, Te-Chin Chu, Chun-Chieh A Shih, Wen-Hsiung Li, Ming-Che Shih
Biotechnology for Biofuels , 2011, DOI: 10.1186/1754-6834-4-24
Abstract: We have developed an efficient platform that uses a combination of transcriptomic and proteomic approaches to N. patriciarum to accelerate gene identification, enzyme classification and application in rice straw degradation. By conducting complementary studies of transcriptome (Roche 454 GS and Illumina GA IIx) and secretome (ESI-Trap LC-MS/MS), we identified 219 putative GH contigs and classified them into 25 GH families. The secretome analysis identified four major enzymes involved in rice straw degradation: β-glucosidase, endo-1,4-β-xylanase, xylanase B and Cel48A exoglucanase. From the sequences of assembled contigs, we cloned 19 putative cellulase genes, including the GH1, GH3, GH5, GH6, GH9, GH18, GH43 and GH48 gene families, which were highly expressed in N. patriciarum cultures grown on different feedstocks.These GH genes were expressed in Pichia pastoris and/or Saccharomyces cerevisiae for functional characterization. At least five novel cellulases displayed cellulytic activity for glucose production. One β-glucosidase (W5-16143) and one exocellulase (W5-CAT26) showed strong activities and could potentially be developed into commercial enzymes.Cellulosic ethanol produced by microbial fermentation from feedstocks has been proposed to replace fossil fuels in transportation. A key step in cellulosic ethanol production is to break down cellulose into glucose and hemicellulose into xylose, which can subsequently be converted into ethanol by fermentative microbes. Therefore, finding efficient cellulases is important to bioethanol production, as well as for hydrolyzing feedstocks into sugars in general. Neocallimastix species is one of the major anaerobic fungi in the rumen of water buffalo capable of efficiently digesting cellulosic biomass [1-4]. Such anaerobic fungi are potential sources for highly active cellulolytic enzymes that are useful for cellulose hydrolysis [5-7]. Plant cell wall degrading enzymes from rumen fungi such as Neocallimastix patriciarum may
Parent-Child Resemblance in Weight Status and Its Correlates in the United States
Yinghui Liu, Hsin-jen Chen, Lan Liang, Youfa Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0065361
Abstract: Background Few studies have examined parent-child resemblance in body weight status using nationally representative data for the US. Design We analyzed Body Mass Index (BMI), weight status, and related correlates for 4,846 boys, 4,725 girls, and their parents based on US nationally representative data from the 2006 and 2007 Medical Expenditure Panel Survey (MEPS). Pearson partial correlation coefficients, percent agreement, weighted kappa coefficients, and binary and multinomial logistic regression were used to examine parent-child resemblance, adjusted for complex sampling design. Results Pearson partial correlation coefficients between parent and child’s BMI measures were 0.15 for father-son pairs, 0.17 for father-daughter pairs, 0.20 for mother-son pairs, and 0.23 for mother-daughter pairs. The weighted kappa coefficients between BMI quintiles of parent and child ranged from ?0.02 to 0.25. Odds ratio analyses found children were 2.1 (95% confidence interval (CI): 1.6, 2.8) times more likely to be obese if only their father was obese, 1.9 (95% CI: 1.5, 2.4) times more likely if only their mother was obese, and 3.2 (95% CI: 2.5, 4.2) times more likely if both parents were obese. Conclusions Parent-child resemblance in BMI appears weak and may vary across parent-child dyad types in the US population. However, parental obesity status is associated with children’s obesity status. Use of different measures of parent-child resemblance in body weight status can lead to different conclusions.
Two-Level Block Decompositions for Solving Helmholtz Equation via Chebyshev Pseudo Spectral Method  [PDF]
Hsin-Chu Chen
Journal of Modern Physics (JMP) , 2018, DOI: 10.4236/jmp.2018.99107
Abstract: In this paper, we consider solving the Helmholtz equation \"\" in the Cartesian domain \"\", subject to homogeneous Dirichlet boundary condition, discretized with the Chebyshev pseudo-spectral method. The main purpose of this paper is to present the formulation of a two-level decomposition scheme for decoupling the linear system obtained from the discretization into independent subsystems. This scheme takes advantage of the homogeneity property of the physical problem along one direction to reduce a 2D problem to several 1D problems via a block diagonalization approach and the reflexivity property along the second direction to decompose each of the 1D problems to two independent subproblems using a reflexive decomposition, effectively doubling the number of subproblems. Based on the special structure of the coefficient matrix of the linear system derived from the discretization and a reflexivity property of the second-order Chebyshev differentiation matrix, we show that the decomposed submatrices exhibits a similar property, enabling the system to be decomposed using reflexive decompositions. Explicit forms of the decomposed submatrices are derived. The decomposition not only yields more efficient algorithm but introduces coarse-grain parallelism. Furthermore, it preserves all eigenvalues of the original matrix.
Block Decompositions and Applications of Generalized Reflexive Matrices  [PDF]
Hsin-Chu Chen
Advances in Linear Algebra & Matrix Theory (ALAMT) , 2018, DOI: 10.4236/alamt.2018.83011
Abstract: Generalize reflexive matrices are a special class of matrices \"\"?that have the relation \"\"where? \"\"and \"\"?are some generalized reflection matrices. The nontrivial cases (\"\" or \"\") of this class of matrices occur very often in many scientific and engineering applications. They are also a generalization of centrosymmetric matrices and reflexive matrices. The main purpose of this paper is to present block decomposition schemes for generalized reflexive matrices of various types and to obtain their decomposed explicit block-diagonal structures. The decompositions make use of unitary equivalence transformations and, therefore, preserve the singular values of the matrices. They lead to more efficient sequential computations and at the same time induce large-grain parallelism as a by-product, making themselves computationally attractive for large-scale applications. A numerical example is employed to show the usefulness of the developed explicit decompositions for decoupling linear least-square problems whose coefficient matrices are of this class into smaller and independent subproblems.
Assessing the Effects of Acupuncture by Comparing Needling the Hegu Acupoint and Needling Nearby Nonacupoints by Spectral Analysis of Microcirculatory Laser Doppler Signals
Hsin Hsiu,Wei-Chen Hsu,Chia-Liang Hsu,Shih-Min Huang
Evidence-Based Complementary and Alternative Medicine , 2011, DOI: 10.1093/ecam/neq073
Abstract: We aimed to assess the effects of acupuncture by analyzing the frequency content of skin blood-flow signals simultaneously recorded at the Hegu acupoint and two nearby nonacupoints following acupuncture stimulation (AS). Laser Doppler flowmetry (LDF) signals were measured in male healthy volunteers in two groups of experiments: needling the Hegu acupoint (n = 13) and needling a nearby nonacupoint (control experiment; n = 10). Each experiment involved recording a 20 min baseline-data sequence and two sets of effects data recorded 0–20 and 50–70 min after stopping AS. Wavelet transform with Morlet mother wavelet was applied to the measured LDF signals. Needling the Hegu acupoint significantly increased the blood flow, significantly decreased the relative energy contribution at 0.02–0.06 Hz and significantly increased the relative energy contribution at 0.4–1.6 Hz at Hegu, but induced no significant changes at the nonacupoints. Also, needling a nearby nonacupoint had no effect in any band at any site. This is the first time that spectral analysis has been used to investigate the microcirculatory blood-flow responses induced by AS, and has revealed possible differences in sympathetic nerve activities between needling the Hegu acupoint and its nearby nonacupoint. One possible weakness of the present design is that different De-Qi feelings following AS could lead to nonblind experimental setup, which may bias the comparison between needling Hegu and its nearby nonacupoint. Our results suggest that the described noninvasive method can be used to evaluate sympathetic control of peripheral vascular activity, which might be useful for studying the therapeutic effects of AS.
Psychiatric Manifestations in Young Females with Congenital Adrenal Hyperplasia in Taiwan
Hsin-Yi Liang,Hsueh-Ling Chang,Ching-Yen Chen,Pei-Yeh Chang
Chang Gung Medical Journal , 2008,
Abstract: Background: Congenital adrenal hyperplasia (CAH) is a syndrome of prenatal and/or postnatalandrogen excess secondary to genetic deficits in the cytochrome p450enzymes of the cortisol synthesis pathway. Women with CAH may sufferfrom different degrees of androgenization. This study documented psychiatricmanifestations in young women born with CAH in Taiwan.Methods: From July 1, 2005 to February 31, 2006 the psychiatric morbidity, genderidentity and sex-typed behavior during childhood of 11 young women withCAH were assessed either using questionnaire-based semi-structured interviewsor self-reported questionnaires.Results: Of the 11 young women (mean age 15.3 5.6 years), 3 (27.3%) had saltwastingCAH and 8 (72.7%) had simple-virilizing CAH. Two patients withoutprior gender assignment (delayed diagnosis) were evaluated for primaryamenorrhea at age 19 and 24 years, respectively. Four of the eleven participants(36.4%) had had an axis I psychiatric diagnosis within the past year.The subjects with more atypical sex-typed behavior in childhood tended tohave a higher risk of minor psychiatric illness (r = –0.706, p < 0.05).Conclusions: This study examined the impact of CAH on the patients’ psychological wellbeing.Psychiatric needs were found to be unmet. Earlier psychiatric evaluationand intervention for these patients is suggested.
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