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Search Results: 1 - 10 of 47380 matches for " Hsiang-En Wu "
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Painful nerve injury increases plasma membrane Ca2+-ATPase activity in axotomized sensory neurons
Geza Gemes, Katherine D Oyster, Bin Pan, Hsiang-En Wu, Madhavi LY Bangaru, Qingbo Tang, Quinn H Hogan
Molecular Pain , 2012, DOI: 10.1186/1744-8069-8-46
Abstract: PMCA function was isolated in dissociated sensory neurons by blocking intracellular Ca2+ sequestration with thapsigargin, and cytoplasmic Ca2+ concentration was recorded with Fura-2 fluorometry. Compared to control neurons, the rate at which depolarization-induced Ca2+ transients resolved was increased in axotomized neurons after spinal nerve ligation, indicating accelerated PMCA function. Electrophysiological recordings showed that blockade of PMCA by vanadate prolonged the action potential afterhyperpolarization, and also decreased the rate at which neurons could fire repetitively.We found that PMCA function is elevated in axotomized sensory neurons, which contributes to neuronal hyperexcitability. Accelerated PMCA function in the primary sensory neuron may contribute to the generation of neuropathic pain, and thus its modulation could provide a new pathway for peripheral treatment of post-traumatic neuropathic pain.
Nitric oxide activates ATP-sensitive potassium channels in mammalian sensory neurons: action by direct S-nitrosylation
Takashi Kawano, Vasiliki Zoga, Masakazu Kimura, Mei-Ying Liang, Hsiang-En Wu, Geza Gemes, J Bruce McCallum, Wai-Meng Kwok, Quinn H Hogan, Constantine D Sarantopoulos
Molecular Pain , 2009, DOI: 10.1186/1744-8069-5-12
Abstract: Cell-attached and cell-free recordings of KATP currents in large DRG neurons from control rats (sham surgery, SS) revealed activation of KATP channels by NO exogenously released by the NO donor SNAP, through decreased sensitivity to [ATP]i.This NO-induced KATP channel activation was not altered in ganglia from animals that demonstrated sustained hyperalgesia-type response to nociceptive stimulation following spinal nerve ligation. However, baseline opening of KATP channels and their activation induced by metabolic inhibition was suppressed by axotomy. Failure to block the NO-mediated amplification of KATP currents with specific inhibitors of sGC and PKG indicated that the classical sGC/cGMP/PKG signaling pathway was not involved in the activation by SNAP. NO-induced activation of KATP channels remained intact in cell-free patches, was reversed by DTT, a thiol-reducing agent, and prevented by NEM, a thiol-alkylating agent. Other findings indicated that the mechanisms by which NO activates KATP channels involve direct S-nitrosylation of cysteine residues in the SUR1 subunit. Specifically, current through recombinant wild-type SUR1/Kir6.2 channels expressed in COS7 cells was activated by NO, but channels formed only from truncated isoform Kir6.2 subunits without SUR1 subunits were insensitive to NO. Further, mutagenesis of SUR1 indicated that NO-induced KATP channel activation involves interaction of NO with residues in the NBD1 of the SUR1 subunit.NO activates KATP channels in large DRG neurons via direct S-nitrosylation of cysteine residues in the SUR1 subunit. The capacity of NO to activate KATP channels via this mechanism remains intact even after spinal nerve ligation, thus providing opportunities for selective pharmacological enhancement of KATP current even after decrease of this current by painful-like nerve injury.Nitric oxide (NO) is a pivotal signaling molecule involved in many diverse developmental and physiological processes in the mammalian nervous system
The C-Terminus of Histone H2B Is Involved in Chromatin Compaction Specifically at Telomeres, Independently of Its Monoubiquitylation at Lysine 123
Chen-Yi Wang, Chia-Yin Hua, Hsiang-En Hsu, Chia-Ling Hsu, Hsin-Yi Tseng, Duncan E. Wright, Pang-Hung Hsu, Chih-Hung Jen, Chia-Yeh Lin, Meng-Ying Wu, Min-Daw Tsai, Cheng-Fu Kao
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0022209
Abstract: Telomeric heterochromatin assembly in budding yeast propagates through the association of Silent Information Regulator (SIR) proteins with nucleosomes, and the nucleosome array has been assumed to fold into a compacted structure. It is believed that the level of compaction and gene repression within heterochromatic regions can be modulated by histone modifications, such as acetylation of H3 lysine 56 and H4 lysine 16, and monoubiquitylation of H2B lysine 123. However, it remains unclear as to whether or not gene silencing is a direct consequence of the compaction of chromatin. Here, by investigating the role of the carboxy-terminus of histone H2B in heterochromatin formation, we identify that the disorderly compaction of chromatin induced by a mutation at H2B T122 specifically hinders telomeric heterochromatin formation. H2B T122 is positioned within the highly conserved AVTKY motif of the αC helix of H2B. Heterochromatin containing the T122E substitution in H2B remains inaccessible to ectopic dam methylase with dramatically increased mobility in sucrose gradients, indicating a compacted chromatin structure. Genetic studies indicate that this unique phenotype is independent of H2B K123 ubiquitylation and Sir4. In addition, using ChIP analysis, we demonstrate that telomere structure in the mutant is further disrupted by a defect in Sir2/Sir3 binding and the resulting invasion of euchromatic histone marks. Thus, we have revealed that the compaction of chromatin per se is not sufficient for heterochromatin formation. Instead, these results suggest that an appropriately arrayed chromatin mediated by H2B C-terminus is required for SIR binding and the subsequent formation of telomeric chromatin in yeast, thereby identifying an intrinsic property of the nucleosome that is required for the establishment of telomeric heterochromatin. This requirement is also likely to exist in higher eukaryotes, as the AVTKY motif of H2B is evolutionarily conserved.
Group Identification on LinkedIn: A Professional Group Study
Johannes K. Chiang,Hung-Yue Suen,Hsiang-En Hsiao
International Business and Management , 2013, DOI: 10.3968/j.ibm.1923842820130601.1020
Abstract: The research investigates how a LinkedIn group affect the factors that lead to group identification and group supportive behaviors. The results of a field study of a LinkedIn HR professional group members suggest that members of LinkedIn groups identify on the basis of selfcategorization, affective commitment, and group selfesteem. In which the group operates and the attractiveness of group members. Also, whereas self-categorization is the most important factor leading to identification for members of the LinkedIn groups. In addition, the study found a strong association between the strength of group identification and the incidence of group supportive behaviors on LinkedIn. The research has important implications for organizations using LinkedIn group to build strong relationships with its members. Key words: LinkedIn group; Group identification; Social identity
Overexpression of Ferredoxin, PETF, Enhances Tolerance to Heat Stress in Chlamydomonas reinhardtii
Yi-Hsien Lin,Kui-You Pan,Ching-Hui Hung,Hsiang-En Huang,Ching-Lian Chen,Teng-Yung Feng,Li-Fen Huang
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141020913
Abstract: Reactive oxygen species (ROS) produced by plants in adverse environments can cause damage to organelles and trigger cell death. Removal of excess ROS can be achieved through the ascorbate scavenger pathway to prevent plant cell death. The amount of this scavenger can be regulated by ferredoxin (FDX). Chloroplastic FDXs are electron transfer proteins that perform in distributing photosynthetic reducing power. In this study, we demonstrate that overexpression of the endogenous photosynthetic FDX gene, PETF, in Chlamydomonas reinhardtii could raise the level of reduced ascorbate and diminish H 2O 2 levels under normal growth conditions. Furthermore, the overexpressing PETF transgenic Chlamydomonas lines produced low levels of H 2O 2 and exhibited protective effects that were observed through decreased chlorophyll degradation and increased cell survival under heat-stress conditions. The findings of this study suggest that overexpression of PETF can increase the efficiency of ROS scavenging in chloroplasts to confer heat tolerance. The roles of PETF in the downregulation of the ROS level offer a method for potentially improving the tolerance of crops against heat stress.
Anisotropic dielectric and ferroelectric response of multiferroic LiCu2O2 in magnetic field
Li Zhao,Kuo-Wei Yeh,Sistla Muralidhara Rao,Tzu-Wen Huang,Phillip Wu,Wei-Hsiang Chao,Chung-Ting Ke,Cheng-En Wu,Maw-Kuen Wu
Physics , 2011, DOI: 10.1209/0295-5075/97/37004
Abstract: LiCu2O2 is the first multiferroic cuprate to be reported and its ferroelectricity is induced by complex magnetic ordering in ground state, which is still in controversy today. Herein, we have grown nearly untwinned LiCu2O2 single crystals of high quality and systematically investigated their dielectric and ferroelectric behaviours in external magnetic fields. The highly anisotropic response observed in different magnetic fields apparently contradicts the prevalent bc- or ab- plane cycloidal spin model. Our observations give strong evidence supporting a new helimagnetic picture in which the normal of the spin helix plane is along the diagonal of CuO4 squares which form the quasi-1D spin chains by edge-sharing. Further analysis suggests that the spin helix in the ground state is elliptical and in the intermediate state the present c-axis collinear SDW model is applicable with some appropriate modifications. In addition, our studies show that the dielectric and ferroelectric measurements could be used as probes for the characterization of the complex spin structures in multiferroic materials due to the close tie between their magnetic and electric orderings.
Taipei's Use of a Multi-Channel Mass Risk Communication Program to Rapidly Reverse an Epidemic of Highly Communicable Disease
Muh-Yong Yen,Tsung-Shu Joseph Wu,Allen Wen-Hsiang Chiu,Wing-Wai Wong,Po-En Wang,Ta-Chien Chan,Chwan-Chuen King
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0007962
Abstract: In September 2007, an outbreak of acute hemorrhagic conjunctivitis (AHC) occurred in Keelung City and spread to Taipei City. In response to the epidemic, a new crisis management program was implemented and tested in Taipei.
Immobilizing topoisomerase I on a surface plasmon resonance biosensor chip to screen for inhibitors
Hsiang-Ping Tsai, Li-Wei Lin, Zhi-Yang Lai, Jui-Yu Wu, Chiao-En Chen, Jaulang Hwang, Chien-Shu Chen, Chun-Mao Lin
Journal of Biomedical Science , 2010, DOI: 10.1186/1423-0127-17-49
Abstract: We established a sensor chip on which the TopI protein is immobilized to evaluate TopI inhibition by SPR. Camptothecin (CPT) targeting the DNA-TopI complex was used as a representative inhibitor to validate this label-free method.Purified recombinant human TopI was covalently coupled to the sensor chip for the SPR assay. The binding of anti-human (h)TopI antibodies and plasmid pUC19, respectively, to the immobilized hTopI was observed with dose-dependent increases in resonance units (RU) suggesting that the immobilized hTopI retains its DNA-binding activity. Neither CPT nor evodiamine alone in the analyte flowing through the sensor chip showed a significant increase in RU. The combination of pUC19 and TopI inhibitors as the analyte flowing through the sensor chip caused increases in RU. This confirms its reliability for binding kinetic studies of DNA-TopI binders for interaction and for primary screening of TopI inhibitors.TopI immobilized on the chip retained its bioactivities of DNA binding and catalysis of intermediates of the DNA-TopI complex. This provides DNA-TopI binders for interaction and primary screening with a label-free method. In addition, this biochip can also ensure the reliability of binding kinetic studies of TopI.DNA topoisomerases (Tops) regulate the topological state of DNA that is crucial for replication transcription, recombination, and other cellular transactions. Mammalian somatic cells express six Top genes: two TopI (TopI and TopImt), two TopII (TopIIα and β), and two TopIII genes (TopIIIα and β) [1]. TopI produces a single-strand break in DNA, allows relaxation of DNA, and then re-ligates it, thus restoring the DNA double strands. The enzymatic mechanism involves two sequential transesterification reactions [2]. In the cleavage reaction, the active site of tyrosine (Tyr723 in human TopI) acts as a nucleophile. A phenolic oxygen attacks a DNA phosphodiester bond, forming an intermediate in which the 3' end of the broken strand is covalentl
An Iterative Geometric Mean Decomposition Algorithm for MIMO Communications Systems
Chiao-En Chen,Chia-Hsiang Yang
Computer Science , 2013,
Abstract: This paper presents an iterative geometric mean decomposition (IGMD) algorithm for multiple-input-multiple-output (MIMO) wireless communications. In contrast to the existing GMD algorithms, the proposed IGMD does not require the explicit computation of the geometric mean of positive singular values of the channel matrix and hence is more suitable for hardware implementation. The proposed IGMD has a regular structure and can be easily adapted to solve problems with different dimensions. We show that the proposed IGMD is guaranteed to converge to the perfect GMD under certain sufficient condition. Three different constructions of the proposed algorithm are proposed and compared through computer simulations. Numerical results show that the proposed algorithm quickly attains comparable performance to that of the true GMD within only a few iterations.
An efficient RNA interference screening strategy for gene functional analysis
Chang Chih-Hung,Wang Hsiang-Iu,Lu Hsiang-Chia,Chen Cheng-En
BMC Genomics , 2012, DOI: 10.1186/1471-2164-13-491
Abstract: Background RNA interference (RNAi) is commonly applied in genome-scale gene functional screens. However, a one-on-one RNAi analysis that targets each gene is cost-ineffective and laborious. Previous studies have indicated that siRNAs can also affect RNAs that are near-perfectly complementary, and this phenomenon has been termed an off-target effect. This phenomenon implies that it is possible to silence several genes simultaneously with a carefully designed siRNA. Results We propose a strategy that is combined with a heuristic algorithm to design suitable siRNAs that can target multiple genes and a group testing method that would reduce the number of required RNAi experiments in a large-scale RNAi analysis. To verify the efficacy of our strategy, we used the Orchid expressed sequence tag data as a case study to screen the putative transcription factors that are involved in plant disease responses. According to our computation, 94 qualified siRNAs were sufficient to examine all of the predicated 229 transcription factors. In addition, among the 94 computer-designed siRNAs, an siRNA that targets both TF15 (a previously identified transcription factor that is involved in the plant disease-response pathway) and TF21 was introduced into orchids. The experimental results showed that this siRNA can simultaneously silence TF15 and TF21, and application of our strategy successfully confirmed that TF15 is involved in plant defense responses. Interestingly, our second-round analysis, which used an siRNA specific to TF21, indicated that TF21 is a previously unidentified transcription factor that is related to plant defense responses. Conclusions Our computational results showed that it is possible to screen all genes with fewer experiments than would be required for the traditional one-on-one RNAi screening. We also verified that our strategy is capable of identifying genes that are involved in a specific phenotype.
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