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Search Results: 1 - 10 of 5994 matches for " Horacio Santiago;Rodrigueiro "
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Digestibilidade verdadeira da lisina HCl e da lisina Sulfato determinada com galos cecectomizados
Neme, Rafael;Albino, Luiz Fernando Teixeira;Rostagno, Horacio Santiago;Rodrigueiro, Ramalho José Barbosa;Nunes, Ricardo Vianna;
Revista Brasileira de Zootecnia , 2001, DOI: 10.1590/S1516-35982001000600021
Abstract: the objective of this experiment was to determine the true digestibility of lysine hcl and lysine sulfate, with cecectomized adult roosters. eighteen roosters, 2.854 kg live weight, were cecectomized and kept in cages for a period of 10 days. after two days of adaptation to the cages the animals were fed twice a day during one hour (8:00 a.m. to 9:00 am and 4:00 p.m. to 5:00 pm), for crop dilation to avoid regurgitation of the feed. the forced fed method was utilized, with 12 roosters placed in individual cages equipped with excreta collection pans. the experiment design was complete randomized, with two treatments (two lysine sources) each one with six replicates. also six birds were fasted to determine the endogenous/metabolic loses. diets and excreta were analyzed to determine amino acid contents and the true lysine digestibility coefficients calculatted. the true digestibility coefficients, in percentage, were 97.59% for lysine hcl and 98.34% for lysine sulfate, they were not statistically different.
Determina??o da Biodisponibilidade da Lisina Sulfato e Lisina HCl com Frangos de Corte
Neme, Rafael;Albino, Luiz Fernando Teixeira;Rostagno, Horacio Santiago;Rodrigueiro, Ramalho José Barbosa;Toledo, Rodrigo Santana;
Revista Brasileira de Zootecnia , 2001, DOI: 10.1590/S1516-35982001000700013
Abstract: with the objective of determine the bioavailability of two lysine sources (lysine hcl and lysine sulfate), by a growth trial, 840 one day old male broiler chicks were placed in 56 boxes. two basal diets were formulated to supply the birds nutritional requirements, one for the starting and the other for the growing period, respectively, deficient only in lysine, which were supplemented 0,08, 0,16 and 0,24% lysine of both lysine sources. weight gain, feed intake, feed conversion, carcass yield, legs quarter yield, breast yield, breast meat yield and abdominal fat content were evaluated. multiple linear regression and the slope ratio technique was used to estimate equations. the bioavailabity of lysine sulfate was obtained as a percentage of lysine hcl considered as 100% available. the equations that best estimated the bioavailability of the lysine sulfate (x2) related to lysine hcl (x1) were: y = 544,72 + 439,62 x1 + 475,84 x2, r2 = 0,90, for weight gain (1 to 21 days of age); y = 1824,63 + 1469,18 x1 + 1381,33 x2, r2 = 0,85, for weight gain from 01 to 42 days of age;11 y = 1,9623 - 0,9043x1 ?1,0235 x2, r2 = 0,83, for feed conversion (1 to 21 days of age); y = 0,3766 + 0,5320 x1 + 0,4986 x2, r2 = 0,88, for breast yield (42 days of age) and y = 0,2565 + 0,4685x1 + 0,4300 x2, r2 = 0,92 for breast meat yield with 42 days of age. the average bioavailability of lysine sulfate was 100,19%, showing that there was no difference (p > 0.05), in the bioavailability of the two lysine sources studied.
Determina o da Biodisponibilidade da Lisina Sulfato e Lisina HCl com Frangos de Corte
Neme Rafael,Albino Luiz Fernando Teixeira,Rostagno Horacio Santiago,Rodrigueiro Ramalho José Barbosa
Revista Brasileira de Zootecnia , 2001,
Abstract: Com o objetivo de determinar a biodisponibilidade de duas fontes de lisina (lisina HCl e lisina sulfato), por intermédio de um ensaio de crescimento, foram alojados em um galp o de alvenaria com 56 boxes 840 pintos de corte machos com um dia de idade. Duas dietas basais foram formuladas para atender as exigências nutricionais das aves nas fases inicial e crescimento, deficientes apenas em lisina e suplementadas em 0,08; 0,16; e 0,24% pelas duas fontes de lisina. As variáveis avaliadas foram: ganho de peso, consumo de ra o, convers o alimentar, rendimento de carca a, rendimento de perna, rendimento de peito, rendimento de filé e porcentagem de gordura abdominal. Com os dados obtidos foram estimadas equa es de regress o linear múltipla e, usando os coeficientes de regress o destas, foi determinada a biodisponibilidade da lisina sulfato em rela o a lisina HCl, padronizada como 100% disponível. As equa es obtidas que melhor estimaram a biodisponibilidade das lisinas foram Y = 544,72 + 439,62 X1 + 475,84 X2, R2 = 0,90, para ganho de peso de 01 a 21 dias de idade, Y = 1824,63 + 1469,18 X1 + 1381,33 X2, R2 = 0,85, para ganho de peso de 01 a 42 dias de idade, Y = 1,9623 - 0,9043X1--1,0235 X2, R2 = 0,83, para convers o alimentar de 01 a 21 dias de idade, Y = 0,3766 + 0,5320 X1 + 0,4986 X2, R2 = 0,88, para peso de peito aos 42 dias de idade e Y = 0,2565 + 0,4685X1 + 0,4300 X2, R2 = 0,92, para peso de filé de peito aos 42 dias de idade das aves. A biodisponibilidade média encontrada para a Lisina Sulfato foi de 100,19%, mostrando n o haver diferen a significativa na biodisponibilidade das lisinas testadas.
Digestibilidade verdadeira da lisina HCl e da lisina Sulfato determinada com galos cecectomizados
Neme Rafael,Albino Luiz Fernando Teixeira,Rostagno Horacio Santiago,Rodrigueiro Ramalho José Barbosa
Revista Brasileira de Zootecnia , 2001,
Abstract: O objetivo deste trabalho foi determinar a digestibilidade verdadeira da lisina HCl e da lisina Sulfato, com galos adultos cecectomizados. Foram utilizados 18 galos Leghorn, cecectomizados, com peso médio de 2,854 kg, e alojados individualmente em gaiolas metálicas durante dez dias. Após os dois primeiros dias de adapta o às baterias, estes animais passaram por um período de cinco dias recebendo ra o em dois períodos diários de uma hora cada (manh , 8 h e tarde, 16 h), com o objetivo de dilata o do papo para evitar regurgita o da ra o a ser introduzida. Foi utilizado o método de alimenta o for ada, com 12 galos alojados individualmente em gaiolas metálicas com bandejas coletoras de excretas. O delineamento experimental foi inteiramente casualisado com dois tratamentos (duas fontes de lisina), com seis repeti es cada. Um ensaio paralelo com 6 aves em jejum foi conduzido para determina o das perdas endógenas/metabólicas das aves. Os teores de aminoácidos das dietas e das excretas foram analisados para a determina o dos coeficientes de digestibilidade verdadeira das lisinas. Os coeficientes de digestibilidade verdadeira, expressos em porcentagem, foram de 97,59% para a lisina HCl e de 98,34% para a lisina sulfato, n o diferindo estatisticamente.
Exigência de metionina + cistina para frangos de corte na fase de crescimento e acabamento
Rodrigueiro Ramalho José Barbosa,Albino Luiz Fernando Teixeira,Rostagno Horacio Santiago,Gomes Paulo Cezar
Revista Brasileira de Zootecnia , 2000,
Abstract: Com o objetivo de determinar as exigências de metionina+cistina, foram utilizados 1440 e 1080 frangos de corte da marca comercial Hubbard, 50% de cada sexo, para as fases de crescimento e acabamento, respectivamente. Seis níveis de suplementa o de DL-metionina (0,0; 0,06, 0,12; 0,18; 0,24; e 0,30%) foram adicionados às ra es basais deficientes em metionina+cistina. Foram avaliados, aos 22-42 e 43-56 dias, ganho de peso, consumo de ra o, convers o alimentar, rendimento e composi o química da carca a, gordura abdominal e concentra o plasmática de ácido úrico. Com base nas análises estatística e nos parametros estudados durante a fase de crescimento (22 a 42 dias de idade), sugere-se utilizar 0,896 e 0,856% de metionina+cistina total para machos e fêmeas, respectivamente. Para a fase de acabamento (43 a 56 dias de idade), com base nas análises estatísticas e nos parametros estudados, sugere-se utilizar 0,764 e 0,740% de metionina+cistina total para machos e fêmeas, respectivamente.
Exigência nutricional de lisina para poedeiras leves e semipesadas nos períodos de 1 a 3 e de 4 a 6 semanas de idade
Rodrigueiro, Ramalho José Barbosa;Rostagno, Horacio Santiago;Albino, Luiz Fernando Teixeira;Gomes, Paulo Cezar;Nunes, Ricardo Vianna;Neme, Rafael;
Revista Brasileira de Zootecnia , 2007, DOI: 10.1590/S1516-35982007000600020
Abstract: with the objective of determine the nutritional requirement of lysine for growing white-egg and brown-egg laying hens in the period from 1 to 3 and 4 to 6 weeks of age, 480 birds were used in the period from 1 to 3 weeks of age and 432 birds in the period from 4 to 6 weeks of age. in both periods, hy-line birds were used; 50% hy-line w 36 (white) and 50% hy-line brown in completely randomized design in a 6 × 2 factorial arrangement (level of lysine [0.00, 0.06, 0.12, 0.18, 0.24, and 0.30%] and strains of laying hens). based on the obtained results for the analyzed variable, the total lysine requirement for the period from 1 to 3 weeks of age was 1.052% for white birds and 0.981% brown birds or 207.38 mg of total lysine/day for white birds and 198.63 mg of total lysine/day for brown birds. for the period from 4 to 6 weeks of age the lysine requirement was 0.939% for white birds and 0.889% for brown birds or 323.30 mg of total lysine/day for white birds and 300.29 mg of total lysine/day for brown birds.
Exigência de metionina + cistina para frangos de corte na fase de crescimento e acabamento
Rodrigueiro, Ramalho José Barbosa;Albino, Luiz Fernando Teixeira;Rostagno, Horacio Santiago;Gomes, Paulo Cezar;Pozza, Paulo Cezar;Neme, Rafael;
Revista Brasileira de Zootecnia , 2000, DOI: 10.1590/S1516-35982000000200026
Abstract: with the objective to determine the methionine+cystine requirements, 1440 and 1080 commercial hubbard broilers were used, 50% of each sex at the growing and finishing phases, respectively. six supplemental levels of dl-methionine (.0, .06, .12, .18, .24, and .30%) were added to methionine+cystine deficient basal diets. body weight gain, feed intake, feed:gain ratio, carcass yield and composition, abdominal fat and plasma uric acid concentration were evaluated from 22-42 and 43-56 days of age. based on the statistical analysis of the studied parameters during the growing phase (22 to 42 days of age), it is suggested to use .896 and .856% of total methionine+cystine for males and females, respectively. for the finishing phase (43 to 56 days of age), based in the statistic analysis and in the studied parameters, it is suggested to use .764% and .740% of total methionine+cystine for males and females, respectively.
Agglutinating Activity and Structural Characterization of Scalarin, the Major Egg Protein of the Snail Pomacea scalaris (d’Orbigny, 1832)
Santiago Ituarte, Marcos Sebastián Dreon, Marcelo Ceolin, Horacio Heras
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0050115
Abstract: Apple snail perivitellins are emerging as ecologically important reproductive proteins. To elucidate if the protective functions of the egg proteins of Pomacea canaliculata (Caenogastropoda, Ampullariidae), involved in embryo defenses, are present in other Pomacea species we studied scalarin (PsSC), the major perivitellin of Pomacea scalaris. Using small angle X-ray scattering, fluorescence and absorption spectroscopy and biochemical methods, we analyzed PsSC structural stability, agglutinating activity, sugar specificity and protease resistance. PsSC aggluttinated rabbit, and, to a lesser extent, human B and A erythrocytes independently of divalent metals Ca2+ and Mg2+ were strongly inhibited by galactosamine and glucosamine. The protein was structurally stable between pH 2.0 to 10.0, though agglutination occurred only between pH 4.0 to 8.0 (maximum activity at pH 7.0). The agglutinating activity was conserved up to 60°C and completely lost above 80°C, in agreement with the structural thermal stability of the protein (up to 60°C). PsSC was able to withstand in vitro gastrointestinal digestion, and showed no trypsin inhibition activity. The presence of lectin activity has been reported in eggs of other Pomacea snails, but here we link for the first time, this activity to an apple snail multifunctional perivitellin. This novel role for a snail egg storage protein is different from closely related P.canaliculata defensive proteins.
The Role of the Proteinase Inhibitor Ovorubin in Apple Snail Eggs Resembles Plant Embryo Defense against Predation
Marcos Sebastián Dreon,Santiago Ituarte,Horacio Heras
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0015059
Abstract: Fieldwork has thoroughly established that most eggs are intensely predated. Among the few exceptions are the aerial egg clutches from the aquatic snail Pomacea canaliculata which have virtually no predators. Its defenses are advertised by the pigmented ovorubin perivitellin providing a conspicuous reddish coloration. The nature of the defense however, was not clear, except for a screening for defenses that identified a neurotoxic perivitellin with lethal effect on rodents.
Lobulectomía pulmonar toracoscópica: resultados iniciales en pediatría
Martínez Ferro,Marcelo; Vidaurreta,Santiago; Buela,Enrique; Bignon,Horacio;
Archivos argentinos de pediatr?-a , 2010,
Abstract: the objective of the present study consists of revising our initial experience with the use of thoracoscopic surgery for lung resections and their complications. clinical histories and videos, from patients treated surgically at the fundación hospitalaria-private children hospital and cemic universitary hospital from april 2005 to october 2008, were retrospectivelly estudied. eleven female and 9 male patients were included. the median surgical time was 150 min (range: 60-270 min). the median time of chest tube permanence was 3 days (range: 2-10 days). median hospital stay was 15 days (range: 3-29 days). it was concluded that thoracoscopic lung lobectomies are technically feasible and applicable to all paediatric ages; they should be considered as an alternative approach for trained groups.
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