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Search Results: 1 - 10 of 8501 matches for " Hongyu Cui "
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Artificial Seeding Effects of Convective Clouds on the Opening Day of Beijing 2008 Summer Olympics  [PDF]
Hongyu Li, Yanping Dai, Hua Wang, Jingang Cui
Journal of Geoscience and Environment Protection (GEP) , 2017, DOI: 10.4236/gep.2017.54010
Abstract: Using the radar reflectivity and intensive rainfall data, artificial seeding effects of convective clouds in Beijing on 8 August 2008, the opening day of the 29th Summer Olympics, were analyzed. The results indicate that, cloud seeding at single operation site for convective clouds invading from southwest direction may sharply mitigate the rainfall observed at leeward automatic weather stations within 5 - 10 min, while enhances the precipitation at a later stage about 10 - 20 min. Cloud seeding effects of operation sites Yuegezhuangxi, Changgouzhen, and Zhoukou, which are placed along the main moving routes or localized developing convective clouds in the west and center parts of Fangshan district, are very conspicuous. Combining the operation sites distribution and radar echoes, it is found that the site Changgouzhen, which is very close to the convective core, plays an essential role in suppressing the growth of convective cloud, reducing the coverage area of intense echoes classified as 45 - 60 dBZ, as well as mitigating the precipitation from neighboring automatic weather stations. Based on radar reflectivity and rainfall data, we find that the clouds over lots of operation sites in eastern Fangshan district are not cold enough to favor glaciogenic seeding with silver iodide, meanwhile, there is not too much precipitation observed.
Capacity Analysis of Bidirectional AF Relay Selection with Imperfect Channel State Information
Hongyu Cui,Rongqing Zhang,Lingyang Song,Bingli Jiao
Mathematics , 2013,
Abstract: In this letter, we analyze the ergodic capacity of bidirectional amplify-and-forward relay selection (RS) with imperfect channel state information (CSI), i.e., outdated CSI and imperfect channel estimation. Practically, the optimal RS scheme in maximizing the ergodic capacity cannot be achieved, due to the imperfect CSI. Therefore, two suboptimal RS schemes are discussed and analyzed, in which the first RS scheme is based on the imperfect channel coefficients, and the second RS scheme is based on the predicted channel coefficients. The lower bound of the ergodic capacity with imperfect CSI is derived in a closed-form, which matches tightly with the simulation results. The results reveal that once CSI is imperfect, the ergodic capacity of bidirectional RS degrades greatly, whereas the RS scheme based on the predicted channel has better performance, and it approaches infinitely to the optimal performance, when the prediction length is sufficiently large.
Relay Selection for Bidirectional AF Relay Network with Outdated CSI
Hongyu Cui,Rongqing Zhang,Lingyang Song,Bingli Jiao
Mathematics , 2013,
Abstract: Most previous researches on bidirectional relay selection (RS) typically assume perfect channel state information (CSI). However, outdated CSI, caused by the the time-variation of channel, cannot be ignored in the practical system, and it will deteriorate the performance. In this paper, the effect of outdated CSI on the performance of bidirectional amplify-and-forward RS is investigated. The optimal single RS scheme in minimizing the symbol error rate (SER) is revised by incorporating the outdated channels. The analytical expressions of end-to-end signal to noise ratio (SNR) and symbol error rate (SER) are derived in a closed-form, along with the asymptotic SER expression in high SNR. All the analytical expressions are verified by the Monte-Carlo simulations. The analytical and the simulation results reveal that once CSI is outdated, the diversity order degrades to one from full diversity. Furthermore, a multiple RS scheme is proposed and verified that this scheme is a feasible solution to compensate the diversity loss caused by outdated CSI.
Performance Analysis of Bidirectional Relay Selection with Imperfect Channel State Information
Hongyu Cui,Rongqing Zhang,Lingyang Song,Bingli Jiao
Computer Science , 2011,
Abstract: In this paper, we investigate the performance of bidirectional relay selection using amplify-and-forward protocol with imperfect channel state information, i.e., delay effect and channel estimation error. The asymptotic expression of end-to-end SER in high SNR regime is derived in a closed form, which indicates that the delay effect causes the loss of both coding gain and diversity order, while the channel estimation error merely affects the coding gain. Finally, analytical results are verified by Monte-Carlo simulations.
Class IIa Bacteriocins: Diversity and New Developments
Yanhua Cui,Chao Zhang,Yunfeng Wang,John Shi,Lanwei Zhang,Zhongqing Ding,Xiaojun Qu,Hongyu Cui
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms131216668
Abstract: Class IIa bacteriocins are heat-stable, unmodified peptides with a conserved amino acids sequence YGNGV on their N-terminal domains, and have received much attention due to their generally recognized as safe (GRAS) status, their high biological activity, and their excellent heat stability. They are promising and attractive agents that could function as biopreservatives in the food industry. This review summarizes the new developments in the area of class IIa bacteriocins and aims to provide uptodate information that can be used in designing future research.
Complete Genome Sequence of the First Chinese Virulent Infectious Laryngotracheitis Virus
Congcong Kong, Yan Zhao, Xianlan Cui, Xiaomin Zhang, Hongyu Cui, Mei Xue, Yunfeng Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070154
Abstract: Infectious laryngotracheitis (ILT) is an acute respiratory disease caused by infectious laryngotracheitis virus (ILTV). The complete genome sequences of five attenuated ILTV vaccine strains and six virulent ILTV strains as well as two Australian ILTV field strains have been published in Australia and the USA so far. To provide the complete genome sequence information of ILTVs from different geographic regions, the whole genome of ILTV LJS09 isolated in China was sequenced. The genome of ILTV LJS09 was 153,201 bp in length, and contained 79 ORFs. Most of the ORFs had high sequence identity with homologous ORFs of reference strains. There was a large fragment deletion within the noncoding region of unique long region (UL) of ILTV LJS09 compared with SA2 and A20 strains. Though the origin binding protein of ILTV LJS09 existed, there was no AT-rich region in strain LJS09. Alignments of the amino acid sequences revealed seven mutations at amino acids 71 (Arg → Lys), 116 (Ala → Val), 207 (Thr → Ile) and 644 (Thr → Ile) on glycoprotein B, 155 (Phe → Ser) and 376 (Arg → His) on glycoprotein D and 8 (Gln→Pro) on glycoprotein L of ILTV LJS09 compared to those of virulent strain (USDA) as ILTV LJS09 did not grow on chicken embryo fibroblasts, suggesting the role of the key seven amino acids in determination of the cell tropism of ILTV LJS09. This is the first complete genome sequence of the virulent strain of ILTV in Asia using the conventional PCR method, which will help to facilitate the future molecular biological research of ILTVs.
Effects of Reticuloendotheliosis Virus Infection on Cytokine Production in SPF Chickens
Mei Xue, Xingming Shi, Yan Zhao, Hongyu Cui, Shunlei Hu, Xianlan Cui, Yunfeng Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0083918
Abstract: Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the Retroviridae family, can result in immunosuppression and subsequent increased susceptibility to secondary infections. The effects of REV infection on expression of mRNA for cytokine genes in chickens have not been completely elucidated. In this study, using multiplex branched DNA (bDNA)?technology, we identified molecular mediators that participated in the regulation of the immune response during REV infection in chickens. Cytokine and chemokine mRNA expression levels were evaluated in the peripheral blood mononuclear cells (PBMCs). Expression levels of interleukin (IL)-4, IL-10, IL-13 and?tumor necrosis factor (TNF)-α were significantly up-regulated while interferon (IFN)-α, IFN-β, IFN-γ, IL-1β,IL-2, IL-3, IL-15, IL-17F, IL-18 and colony-stimulating factor (CSF)-1 were markedly decreased in PBMCs at all stages of infection. Compared with controls, REV infected chickens showed greater expression levels of IL-8 in PBMCs 21 and 28 days post infection. In addition, REV regulates host immunity as a suppressor of T cell proliferative responses. The results in this study will help us to understand the host immune response to virus pathogens.
Identification of a Conserved B-cell Epitope on Reticuloendotheliosis Virus Envelope Protein by Screening a Phage-displayed Random Peptide Library
Mei Xue, Xingming Shi, Jing Zhang, Yan Zhao, Hongyu Cui, Shunlei Hu, Hongbo Gao, Xianlan Cui, Yun-Feng Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0049842
Abstract: Background The gp90 protein of avian reticuloendotheliosis-associated virus (REV-A) is an important envelope glycoprotein, which is responsible for inducing protective antibody immune responses in animals. B-cell epitopes on the gp90 protein of REV have not been well studied and reported. Methods and Results This study describes the identification of a linear B-cell epitope on the gp90 protein by screening a phage-displayed 12-mer random peptide library with the neutralizing monoclonal antibody (mAb) A9E8 directed against the gp90. The mAb A9E8 recognized phages displaying peptides with the consensus motif SVQYHPL. Amino acid sequence of the motif exactly matched 213SVQYHPL219 of the gp90. Further identification of the displayed B cell epitope was conducted using a set of truncated peptides expressed as GST fusion proteins and the Western blot results indicated that 213SVQYHPL219 was the minimal determinant of the linear B cell epitope recognized by the mAb A9E8. Moreover, an eight amino acid peptide SVQYHPLA was proven to be the minimal unit of the epitope with the maximal binding activity to mAb A9E8. The REV-A-positive chicken serum reacted with the minimal linear epitopes in Western blot, revealing the importance of the eight amino acids of the epitope in antibody-epitope binding activity. Furthermore, we found that the epitope is a common motif shared among REV-A and other members of REV group. Conclusions and Significance We identified 213SVQYHPL219 as a gp90-specific linear B-cell epitope recognized by the neutralizing mAb A9E8. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against REV-A and other viruses of the REV group.
Expression of HA of HPAI H5N1 Virus at US2 Gene Insertion Site of Turkey Herpesvirus Induced Better Protection than That at US10 Gene Insertion Site
Hongbo Gao, Hongyu Cui, Xianlan Cui, Xingming Shi, Yan Zhao, Xiaoyan Zhao, Yanming Quan, Shuai Yan, Weiwei Zeng, Yunfeng Wang
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0022549
Abstract: Herpesvirus of turkey (HVT) is being widely used as a vector for development of recombinant vaccines and US2 and US10 genes are often chosen as insertion sites for targeted gene expression. However, the different effects of the two genes for generation of recombinant HVT vaccines were unknown. In order to compare the effects of inserted genes in the two sites on the efficacy of the recombinant vaccines, host-protective haemagglutinin (HA) gene of the highly pathogenic avian influenza virus (HPAIV) H5N1 was inserted into either US2 or US10 gene locus of the HVT. The resulting US2 (rHVT-US2-HA) or US10 (rHVT-US10-HA) recombinant HVT viruses were used to infect chicken embryo fibroblasts. Plaques and the growth kinetics of rHVT-US2-HA-infected chicken embryo fibroblasts were similar to those of parental HVT whereas rHVT-US10-HA infected chicken embryo fibroblasts had different growth kinetics and plaque formation. The viremia levels in rHVT-US10-HA virus-infected chickens were significantly lower than those of rHVT-US2-HA group on 28 days post infection. The vaccine efficacy of the two recombinant viruses against H5N1 HPAIV and virulent Marek's disease virus was also evaluated in 1-day-old vaccinated chickens. rHVT-US2-HA-vaccinated chickens were better protected with reduced mortality than rHVT-US10-HA-vaccinated animals following HPAIV challenge. Furthermore, the overall hemaglutination inhibition antibody titers of rHVT-US2-HA-vaccinated chickens were higher than those of rHVT-US10-HA-vaccinated chickens. Protection levels against Marek's disease virus challenge following vaccination with either rHVT-US2-HA or rHVT-US10-HA, however, were similar to those of the parental HVT virus. These results, for the first time, indicate that US2 gene provides a favorable foreign gene insertion site for generation of recombinant HVT vaccines.
Detection of Infectious Laryngotracheitis Virus by Real-Time PCR in Naturally and Experimentally Infected Chickens
Yan Zhao, Congcong Kong, Xianlan Cui, Hongyu Cui, Xingming Shi, Xiaomin Zhang, Shunlei Hu, Lianwei Hao, Yunfeng Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0067598
Abstract: Infectious laryngotracheitis (ILT) is an acute, highly contagious upper-respiratory infectious disease of chickens. In this study, a real-time PCR method was developed for fast and accurate detection and quantitation of ILTV DNA of chickens experimentally infected with ILTV strain LJS09 and naturally infected chickens. The detection lower limit of the assay was 10 copies of DNA. There were no cross reactions with the DNA and RNA of infectious bursal disease virus, chicken anemia virus, reticuloendotheliosis virus, avian reovirus, Newcastle disease virus, and Marek's disease virus. The real-time PCR was reproducible as the coefficients of variation of reproducibility of the intra-assay and the inter-assay were less than 2%. The real-time PCR was used to detect the levels of the ILTV DNA in the tissues of specific pathogen free (SPF) chickens infected with ILTV at different times post infection. ILTV DNA was detected by real-time PCR in the heart, liver, spleen, lung, kidney, larynx, tongue, thymus, glandular stomach, duodenum, pancreatic gland, small intestine, large intestine, cecum, cecal tonsil, bursa of Fabricius, and brain of chickens in the infection group and the contact-exposure group. The sensitivity, specificity, and reproducibility of the ILTV real-time PCR assay revealed its suitability for detection and quantitation of ILTV in the samples from clinically and experimentally ILTV infected chickens.
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