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Search Results: 1 - 10 of 1250 matches for " Hisashi Harada "
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Biocatalytic synthesis of flavones and hydroxyl-small molecules by recombinant Escherichia coli cells expressing the cyanobacterial CYP110E1 gene
Takuya Makino, Toshihiko Otomatsu, Kazutoshi Shindo, Emi Kitamura, Gerhard Sandmann, Hisashi Harada, Norihiko Misawa
Microbial Cell Factories , 2012, DOI: 10.1186/1475-2859-11-95
Abstract: Escherichia coli cells carrying each P450 gene that was inserted into the pRED vector, containing the RhFRed reductase domain sequence from Rhodococcus sp. NCIMB 9784 P450RhF (CYP116B2), were co-cultured with substrates and products were identified when bioconversion reactions proceeded. Consequently, CYP110E1 of Nostoc sp. strain PCC 7120, located in close proximity to the first branch point in the phylogenetic tree of the CYP110 family, was found to be promiscuous for the substrate range mediating the biotransformation of various small molecules. Naringenin and (hydroxyl) flavanones were respectively converted to apigenin and (hydroxyl) flavones, by functioning as a flavone synthase. Such an activity is reported for the first time in prokaryotic P450s. Additionally, CYP110E1 biotransformed the notable sesquiterpene zerumbone, anti-inflammatory drugs ibuprofen and flurbiprofen (methylester forms), and some aryl compounds such as 1-methoxy and 1-ethoxy naphthalene to produce hydroxylated compounds that are difficult to synthesize chemically, including novel compounds.We elucidated that the CYP110E1 gene, C-terminally fused to the P450RhF RhFRed reductase domain sequence, is functionally expressed in E. coli to synthesize a robust monooxygenase, which shows promiscuous substrate specificity (affinity) for various small molecules, allowing the biosynthesis of not only flavones (from flavanones) but also a variety of hydroxyl-small molecules that may span pharmaceutical and nutraceutical industries.
Paclitaxel-Induced Apoptosis Is BAK-Dependent, but BAX and BIM-Independent in Breast Tumor
Anna V. Miller, Mark A. Hicks, Wataru Nakajima, Amanda C. Richardson, Jolene J. Windle, Hisashi Harada
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0060685
Abstract: Paclitaxel (Taxol)-induced cell death requires the intrinsic cell death pathway, but the specific participants and the precise mechanisms are poorly understood. Previous studies indicate that a BH3-only protein BIM (BCL-2 Interacting Mediator of cell death) plays a role in paclitaxel-induced apoptosis. We show here that BIM is dispensable in apoptosis with paclitaxel treatment using bim?/? MEFs (mouse embryonic fibroblasts), the bim?/? mouse breast tumor model, and shRNA-mediated down-regulation of BIM in human breast cancer cells. In contrast, both bak?/? MEFs and human breast cancer cells in which BAK was down-regulated by shRNA were more resistant to paclitaxel. However, paclitaxel sensitivity was not affected in bax?/? MEFs or in human breast cancer cells in which BAX was down-regulated, suggesting that paclitaxel-induced apoptosis is BAK-dependent, but BAX-independent. In human breast cancer cells, paclitaxel treatment resulted in MCL-1 degradation which was prevented by a proteasome inhibitor, MG132. A Cdk inhibitor, roscovitine, blocked paclitaxel-induced MCL-1 degradation and apoptosis, suggesting that Cdk activation at mitotic arrest could induce subsequent MCL-1 degradation in a proteasome-dependent manner. BAK was associated with MCL-1 in untreated cells and became activated in concert with loss of MCL-1 expression and its release from the complex. Our data suggest that BAK is the mediator of paclitaxel-induced apoptosis and could be an alternative target for overcoming paclitaxel resistance.
Cooperative Phenomenon of Ferromagnetism and Unconventional Superconductivity in UGe$_2$: A ^73^Ge-NQR Study under Pressure
Atsushi Harada,Shinji Kawasaki,Hisashi Kotegawa,Yoshio Kitaoka,Yoshinori Haga,Etsuji Yamamoto,Yoshichika Onuki,Kouhei Itoh,Eugene Haller,Hisatomo Harima
Physics , 2005, DOI: 10.1143/JPSJ.74.2675
Abstract: We report on a cooperative phenomenon of ferromagnetism and unconventional superconductivity (SC) in UGe$_2$ through the measurements of $^{73}$Ge nuclear-quadrupole-resonance (NQR) under pressure ($P$). The NQR spectra evidenced phase separation into ferromagnetic and paramagnetic phases in the vicinity of $P_c\sim 1.5$ GPa, pointing to a first-order transition. The measurements of nuclear-spin-lattice-relaxation-rate $1/T_1$ revealed that SC emerges under the background of ferromagnetism, but not of the paramagnetic phase.
73Ge-NMR/NQR Investigation of Magnetic Properties of URhGe
Hisashi Kotegawa,Kenta Fukumoto,Toshihiro Toyama,Hideki Tou,Hisatomo Harima,Atsushi Harada,Yoshio Kitaoka,Yoshinori Haga,Etsuji Yamamoto,Yoshichika Onuki,Kohei M. Itoh,Eugene E. Haller
Physics , 2015,
Abstract: We report 73Ge-NMR and NQR results for ferromagnetic (FM) superconductor URhGe. The magnitude and direction of the internal field, H_int, and parameters of the electric field gradient at the Ge site were determined experimentally. Using powdered polycrystalline samples oriented by different methods, the field dependences of NMR shift and nuclear spin relaxation rates for H_0 // c (easy axis) and H_0 // b were obtained. From the NMR shifts for H_0 // b, we confirmed a gradual suppression of the Curie temperature and observed a phase separation near the spin reorientation. The observation of the phase separation gives microscopic evidence that the spin reorientation under H_0 // b is of first order at low temperatures. The nuclear spin-lattice relaxation rate 1/T_1 indicates that the magnetic fluctuations are suppressed for H_0 // c, whereas the fluctuations remain strongly for H_0 // b. The enhancements of both 1/T_1T and the nuclear spin-spin relaxation rate 1/T_2 for H_0 // b toward the spin reorientation field suggest that the field-induced superconductivity in URhGe emerges under the magnetic fluctuations along the b axis and the c axis.
Study on Digitization of TCM Diagnosis Applied Extraction Method of Blood Vessel  [PDF]
Cong Wu, Koichi Harada
Journal of Signal and Information Processing (JSIP) , 2011, DOI: 10.4236/jsip.2011.24043
Abstract: This paper presents a study on digitization of Traditional Chinese Medicine diagnosis. The research consists of two aspects: a) algorithms for blood vessels extraction in sclera-conjunctiva images, which can be applied in syndrome differentiation by observing human eyes (named Ocular Diagnostic in Traditional Chinese Medicine); b) digitization of extracted vessels. First, sclera-conjunctiva region is isolated by optimal threshold segmentation and mathematical operation; Scanning and edge detection methods are used to gain the edge of the blood vessels. Moreover, the edge feature parameters are gained, which can be used to reconstruct the blood vessels. Experimental results show that blood vessels information can be obtained fast and accurately for the further TCM diagnosis by artificial system.
State of Art of Ants (Hymenoptera: Formicidae) at Caxiuan?, Melgaco, Pará, Brazil  [PDF]
Ana Yoshi Harada
Advances in Entomology (AE) , 2016, DOI: 10.4236/ae.2016.43013
Abstract: The ants are one of the mega diverse insets found in all terrestrial environments, and occur in great diversity, richness and abundance, acting at all trophic levels. The present study was conducted to verify the knowledge of ant fauna at Caxiuan? National forest. All available ant literature and some collection in Caxiuan? were compiled and analyzed in EXCEL program. Caxiuan? is one Brazilian Amazonia forest that has good biological conservation. A total of 197 species belonging to 52 genera of the nine subfamilies are present in Caxiuan?. Many studies need to be done in this place to understand the ant community pattern to give base for other studies and proposal for use, management and conservation of this interesting Amazonia place.
Statistical Survey of Deaths from Nonmelanoma Skin Cancer in Japan during 54 Years
Hisashi Ohtsuka
Journal of Skin Cancer , 2011, DOI: 10.1155/2011/293926
Abstract: The author analyzed the annual trends in the number of deaths from nonmelanoma skin cancer (NMSC) from 1955 to 2008 in Japan on the basis of the data from the Vital Statistics of Japan. The general trends in the number of deaths from NMSC were downward between 1979 to 1994, but upward after 1995. The general trends in age-standardized death rates were roughly downward, although the death rates plateaued after 1995. The recent annual increased ratio of deaths from NMSC was 3.8% (95% confidence interval: 2.7?~?4.9%). The number and proportion of deaths from NMSC among the elderly were increasing in Japan. For females, more than 50% of the deaths occurred recently at or after 85?years of age, whereas, for males, this proportion was at or after 75?years of age, nearly reaching at or after 80?years of age. 1. Introduction The incidence of nonmelanoma skin cancer (NMSC) has been increasing during the past half century in many countries [1–6]. Authors performed the analysis of changing trends in the number of deaths from NMSC in Japan from 1955 to 2000 [7]. This time, the author expanded the period of survey to 2008 and revised the analysis, using the new WHO standard population which is effective for the period 2000–2025 [8]. 2. Material and Methods The annual trends in the number of deaths from NMSC in Japan from 1955 to 2008, those in age-standardized death rates, those in 3-year moving average, those by 5-year age group, those by sex and age group, and those in the proportion of deaths by sex and age group were investigated on the basis of the data from the Vital Statistics of Japan, Statistics and Information Department, Minister's Secretariat, Ministry of Health, Labor and Welfare. The corresponding revisions of the International Classification of Diseases (ICD) and codes were ICD-6 code 191 in 1955–1957, ICD-7 code 191 in 1958–1967, ICD-8 code 173 in 1968–1978, ICD-9 code 173 in 1979–1994, and ICD-10 code C44 in 1995–2008, respectively. The author, however, treated the results continuously from 1955 to 1967, because there was no difference between ICD-6 and ICD-7 concerning NMSC. The author treated the remaining revisions as discontinuous, because there was a minor difference between ICD-6/-7, ICD-8, ICD-9, and ICD-10. The author judged that it would be possible to obtain the general annual trends in the number of deaths from NMSC in Japan for 54 years, although, strictly speaking, there were four times minor changes of ICD classifications concerning NMSC between these periods. The death rates were adjusted every year using the new WHO world standard
Production of gold-198 grains
Hisashi Kato
Gold Bulletin , 1978, DOI: 10.1007/BF03215093
On Development of an Adaptive Tutoring System Utilizing Educator's Knowledge Structure
Hisashi Yokota
Lecture Notes in Engineering and Computer Science , 2011,
A Problem Based Learning System for Linear Algebra with Simple Input Method
Hisashi Yokota
Lecture Notes in Engineering and Computer Science , 2010,
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