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We have developed a novel metal tag, bis(ethylenediamine)-4'-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester (ECRS) for sensitive analysis of amino acids using high performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS). ECRS is a functional reagent, containing an ester group at one end that can be activated to bind to amino group and a chelated ruthenium at the other. The activated ester was reacted briefly with amino groups under weakly alkaline conditions. The ruthenium was detected sensitively by ICP-MS. ECRS was reacted with 17 proteinogenic amino acids in borate buffer. The derivatives were separated by reversed phase HPLC and identified by quadrupole-based ICP-MS. ECRS was suitable for speciation; low molecular weight compounds containing amino groups. We have thus established a quantitative analytical method for amino acids and dipeptides. The detection limits of branched amino acids (signal-to-noise ratio of 3) were 1.5 nmol·L-1 in the standard solution (100 amol per injection).
adverse effects of lead poisoning have long been of environmental concern. A
large number of research papers have reported many routes of lead poisoning, of
which lead sinkers used in fishing require urgent attention. Lead sinkers that
are abandoned or scattered from individual fishing gear are notorious for
environmental pollution and have been reported in many papers; moreover,
lead-core “sinker rope” is used in fishery nets. This paper discusses the
latter, which generally has limited public awareness. Preliminary work and
field studies were conducted to develop a system for total treatment of lead
from fishing nets with lead-cored sinker ropes, in order to prevent
environmental pollution and conserve natural resources. Lead sinkers in fishing
nets were successfully removed, yielding high-quality lead suitable for
commercial purposes. Appropriate management of fishing nets is crucial for
environmental protection and resource conservation.