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Search Results: 1 - 10 of 185854 matches for " Helena Marques Fonseca de; "
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Do DNA extraction methods and Taq polimerase quality improve the double repetitive element (DRE) PCR typing method for Mycobacterium tuberculosis strains?
Cavalcanti, Hebe Rodrigues;Marques, Elizabeth;Fonseca, Leila de Souza;Saad, Maria Helena Féres;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000300004
Abstract: double repetitive element (dre) pcr amplification is a simple mycobacterium tuberculosis typing method, however amplification failure or poor resolution of bands commit its efficacy. in order to verify if whether or not these features could be minimized by improving dna extraction procedures or taq polymerise quality, dre-pcr was performed on 24 m. tuberculosis dna samples extracted by heat-shock, mechanical and enzymatic methods applying conventional and hot start taq pol. we demonstrated that when dealing with the mycobacterium tuberculosis dre-pcr typing method, taq pol of better quality might be more important to improve amplification than the dna extraction method.
Bacterial translocation in rats nonfunctioning diverted distal colon
Pinto Júnior, Francisco Edilson Leite;Brandt, Carlos Teixeira;Medeiros, Aldo da Cunha;Oliveira, Ariano José Freitas de;Jer?nimo, Selma Maria;Brito, Helena Marques Fonseca de;
Acta Cirurgica Brasileira , 2007, DOI: 10.1590/S0102-86502007000300007
Abstract: purpose: to investigate whether the alterations of the diverted colon segment mucosa, evidenced in fecal colitis, would be able to alter bacterial translocation (bt). methods: sixty-two wistar male rats ranging from 220 to 320 grams of weight, were divided in two groups: a (colostomy) and b (control), with 31 animals each one. in group a, all animals underwent end colostomy, one stoma, in ascending colon; and in the 70th pod was injected in five rats, by rectal route diverted segment - 2ml of a 0.9% saline solution in animals (a1 subgroup); in eight it was inoculated, by rectal route, 2ml of a solution containing escherichia coli atcc 25922 (american type culture collection), in a concentration of 108 colony forming unit for milliliters (cfu/ml) - a2 subgroup; in ten animals the same solution of e. coli was inoculated, in a concentration of 1011 cfu/ml (a3 subgroup); and in eight it was collected part of the mucus found in the diverted distal colonic segment for neutral sugars and total proteins dosage (a4 subgroup). the animals from the group b underwent the same procedures of group a, but with differences in the colostomy confection. in rats from subgroups a1, a2, a3, b1, b2, and b3 2ml of blood were aspirated from the heart, and fragments from mesenteric lymphatic nodule, liver, spleen, lung and kidney taken for microbiological analysis, after their death. this analysis consisted of evidencing the presence of e. coli atcc 25922 cfu. mann-whitney and anova tests were applied as analytic techniques for association of variables. results: the occurrence of bt was evidenced only in those animals in which inoculated concentration of e. coli atcc 25922, reached levels of 1011cfu/ml, i.e. in subgroups a3 and b3, although, being significantly greater (80%) in those animals without colostomy (subgroup b3) when compared to the ones with colostomy (20%) from the subgroup a3 (p <0.05). lung, liver and mesenteric lymphatic nodules were the tissues with larger percentile of bac
Avalia??o crítica dos efeitos adversos do tratamento anti-retroviral no feto, recém-nascido e lactente
Marques, Heloisa Helena de Sousa;
Revista Brasileira de Ginecologia e Obstetrícia , 2006, DOI: 10.1590/S0100-72032006000700008
Abstract: the implementation of prophylactic antiretroviral drugs and others strategies to prevent vertical hiv transmission in brazil has reduced the number of newly hiv-infected children. since these drugs are only recently available for treatment, there is no data of conclusive safety during pregnancy and fetus and newborn. this article reviews the possible effects of these drugs in fetus, newborn and childhood, including teratogenesis, carcinogenesis and toxicity. in the literature there are an increasing description of adverse effects such as mithocondrial toxicity, neurological symptoms, blood and liver toxicity in hiv-exposed not infected children. antiretroviral treatment recommendations are based on principles that known benefit to the pregnat woman should be mantained unless adverse effects outweight the benefit. a long term follow up of hiv- exposed children is proposed to better understanding of these potential toxicity. in this review the author suggests a practical clinical and laboratory routinary evaluation during the first years of life until adolescence for hiv-exposed not infected children.
Estudo soroepidemiológico da cisticercose humana em um município do Estado do Piauí, Regi?o Nordeste do Brasil
Ramos Jr., Alberto Novaes;Macedo, Heloísa Werneck de;Rodrigues, Marcelo Campos;Peralta, Regina Helena Saramago;Macedo, Nicodemos Alves de;Marques, Margareth da Cunha;Alves, Jair Rodrigues;Paes, Arly de Negreiros;Castro, José Adail Fonseca de;Araújo, Adauto José Gon?alves;Peralta, José Mauro;
Cadernos de Saúde Pública , 2004, DOI: 10.1590/S0102-311X2004000600012
Abstract: as part of parasitological studies in the area surrounding the serra da capivara national park, piauí state, northeast brazil, from 1999 to 2001, the current study aimed to evaluate the epidemiological profile of human cysticercosis in the municipality of jo?o costa. clinical and epidemiological data were obtained, and blood samples were drawn for immunoenzymatic serological tests (elisa and western blot), using taenia crassiceps as the antigen. the first stage, in 1999, investigated 169 individuals with a confirmed history or suspicion of infection/disease involving the teniasis/cysticercosis complex, along with the family members. some 13.6% of the individuals were seroreactive for cysticercosis by the elisa method. the second stage, in 2001, evaluated 92 serum samples of individuals who had been detected as reactive for cysticercosis in the first stage, along with their family members; 24% of the samples were reactive to cysticercosis by elisa and 29% by western blot. during this same stage a coprological survey was performed with 701 individuals, including volunteers. prevalence of intestinal parasites was 51%, with a higher prevalence of protozoans (95%) than helminths (5%). the results indicate the endemicity of cysticercosis in the area, in addition to the high frequency of intestinal protozoan infections.
The Contribution of Waves in Mixing Processes of the Patos Lagoon Plume  [PDF]
Eduardo de Paula Kirinus, Wiliam Correa Marques, Julliet Correa da Costa, Elisa Helena Le?o Fernandes
International Journal of Geosciences (IJG) , 2012, DOI: 10.4236/ijg.2012.35102
Abstract: The analysis of wave effects above salinity is important in order to define mixing processes and their potential to change density gradients, since action in the region of the Patos Lagoon plume is highly dynamic. This paper aims to evaluate the influence of waves on the mixing pattern of the adjacent continental shelf. Constantly marked by tides and circulation variations, changing from flood to ebb tide, salinity stands as a major parameter for analysis. The study is based on hydrodynamic numerical modeling experiments using the TELEMAC3D model and waves generated by the wind using the TOMAWAC coupled model. The comparison between the situations considering the waves generated by the wind and without waves was made through time series analysis, stratification series, transversal cross sections, longitudinal cross sections, salinity mean fields and diagrams by Richardson and Brünt-V?is?l?s frequency parameters. With the cross sections analysis, the mixing effect generated by the waves was observed, demonstrating clearly that the waves were capable of raising the superficial salinity in regions far from the Patos Lagoon entrance, while at the entrance, the wave effects enhanced the mixing on the deepest layers, decreasing the saline intrusion and expanding the plume more radially on the surface. The space-temporal diagrams were effective to demonstrate the modulation effect of the waves over the salt mixing of saline waters, revealing that wave effects decrease the stratification as well as the Brünt-V?is?l?s frequency and increase the period of buoyancy, due to the mixing increase.
A inferência lexical no texto técnico em inglês
Maria Helena Guimar?es Marques de Souza
Alfa : Revista de Linguística , 2001,
Abstract: O objetivo deste trabalho é investigar a estratégia de inferência lexical de palavras-chave em textos técnicos de língua inglesa. Os sujeitos da pesquisa s o alunos de um curso técnico de Eletr nica, falsos principiantes na língua estrangeira. A análise dos dados é quantitativa e qualitativa. A pesquisa observa o papel do conhecimento prévio dos sujeitos sobre assuntos específicos e as pistas contextuais oferecidas pelo autor de textos técnicos, na inferência lexical. As categorias de familiariza o lexical estudadas s o: explica o, defini o, exemplifica o, ilustra o, estipula o e sinonímia. Os resultados demonstram uma certa preponderancia das estratégias de uso do conhecimento prévio sobre as de uso de pistas contextuais, embora a pesquisa tenha constatado que a intera o das duas estratégias seja importante para que a inferência lexical seja produzida com maior sucesso.
A promo??o do desenvolvimento local apoiada em redes de municípios: a experiência do Consórcio Intermunicipal Central Paulista
Lorenzo, Helena Carvalho De;Fonseca, Sergio de Azevedo;
Intera??es (Campo Grande) , 2008, DOI: 10.1590/S1518-70122008000100006
Abstract: the paper presents the construction of the paulista centre intermunicipal partnership brasil-itália, formed by the municipality of araraquara, ibaté, gavi?o peixoto, ribeir?o bonito and s?o carlos. it discusses the possibility of local public policies implementation in the region, the dificulties in using preconceived models, and the potencial and limit of regional development public policy.
Individual factors related to chronic condition in Portuguese adolescents: Highlights from the HBSC/WHO study  [PDF]
Teresa Santos, Margarida Gaspar de Matos, Maria Celeste Sim?es, Helena Fonseca, Maria do Céu Machado
Health (Health) , 2013, DOI: 10.4236/health.2013.511A2005

Background: Adolescence includes a period (10 to 19 years) of profound biopsychosocial changes, constituting potentially difficult challenges, which may become more pronounced in the presence of a chronic condition and its limitations. Responses are not homogeneous and can be quite variable, depending on various specific individual factors. Research comparing adolescents with or without chronic illness, or comparing across different conditions, has been contradictory, not confirming a direct relationship between the degree of suffering and the chronic condition. Objective: To characterize and assess the impact of 1) having a chronic condition (CC) and 2) how CC affects school participation; and its association with life satisfaction and perception of wellness, controlling for demographic factors: age, gender and family socioeconomic status (SES). Methods: 5050 Portuguese adolescents with an average age of 14 years participated in the Health Behaviour in School-aged Children/WHO (HBSC). Results: The majority of the adolescents with CC reported that their conditions did not affect school participation. Adolescents with CC who indicated that CC affected school participation felt more frequently unwell and presented lower life satisfaction. Being a boy, younger and having high family socio economic status (SES) were identified as predictors of higher life satisfaction; on the other hand, being a girl, older, having lower SES, living with CC and feeling that CC affects school participation are predictors for feeling more frequently unwell. Conclusions: These

Madelung's disease as a rare cause of obstructive sleep apnea
Fonseca, Vitor Alexandre Oliveira;Alves, Carlos;Marques, Helena;Camacho, Elvira;Saraiva, António Pinto;
Jornal Brasileiro de Pneumologia , 2009, DOI: 10.1590/S1806-37132009001000013
Abstract: madelung's disease, or multiple symmetric lipomatosis, is a rare disease, characterized by accumulation of unencapsulated fat, generally located symmetrically around the neck and shoulders. here, we present the case of a patient with diffuse lipomatosis accompanied by obstructive sleep apnea due to cervical involvement and facial deformity, which made it necessary to use nasal pillows for ventilation. the patient was hospitalized with a diagnosis of pneumonia and required noninvasive ventilation due to severe hypercapnia. a brief review of the literature was made, and we describe and discuss the investigation of this rare clinical case.
Dificuldades políticas, éticas e jurídicas na cria??o e aplica??o da legisla??o sobre álcool e tabaco: contributo para o desenvolvimento da investiga??o em Direito da Saúde Pública
Cunha Filho,Hilson; Marques,Rita Fonseca; Faria,Paula Lobato de;
Revista Portuguesa de Saúde Pública , 2010,
Abstract: introduction: the application of laws of public health in the area of health determinants related to lifestyles and behaviors, which include the fight against harmful use of alcohol and tobacco control, needs to be analyzed and discussed taking into account the very particular complexity of this reality, where it is not possible to decouple the action to be taken from the various social stakeholders involved. indeed, in these areas, the lobbying activities of industry and distribution and sales sectors, show the existence of competing interests with the policies of public health, interfering in the results of these and preventing their effectiveness. material and methods: taking as a background the above mentioned assumption, this article presents a brief reflection on the strengths and weaknesses of the use of legal instruments in public health, taking as example the portuguese laws on alcohol and tobacco, for which situations of ambiguity and imprecision in its enforcement and a lack of effective monitoring and sanctioning of its non-compliance have put a stop to a effectiveness of the same legislation in practice. the article also shows recent foreign examples which illustrate the pitfalls that can arise even in public health laws with clear objectives to reduce the consumption of health hazardous substances and their potential harm. such is the case with u.s. law no. 111-31 of 22 june 2009 which gave the fda (food and drug administration) the power to control tobacco products and has been the aim of harsh criticism from public health authorities and academics. in this article, although affirming the undeniable power of the legislative instrument to change attitudes and behaviors we, express, however, some concerns respecting the fact that the law is still an unexplored and poorly evaluated instrument in the public health domain. to identify the strengths and weaknesses in the laws concerning public health is indeed as complex as the multidisciplinary sciences an
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