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Search Results: 1 - 10 of 8060 matches for " Helena Kaminami;Reiche "
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Seroprevalence for hepatitis E virus (HEV) infection among volunteer blood donors of the Regional Blood Bank of Londrina, State of Paraná , Brazil
Bortoliero, André Luiz;Bonametti, Ana Maria;Morimoto, Helena Kaminami;Matsuo, Tiemi;Reiche, Edna Maria Vissoci;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2006, DOI: 10.1590/S0036-46652006000200006
Abstract: a cross-sectional study was carried out among 996 volunteer blood donors enrolled from may 1999 to december 1999 to determine the seroprevalence of hepatitis e virus (hev) infection among volunteer blood donors of the regional blood bank of londrina, state of paraná, brazil, and to evaluate whether the rate of seroprevalence of igg anti-hev antibodies is associated with sociodemographic variables and with seropositivity for hepatitis a virus (hav) infection. all participants answered the questionnaire regarding the sociodemographic characterisitcs. serum samples were tested for igg antibodies to hev (anti-hev) by an enzyme linked immunoassay (elisa). all serum samples positive for anti-hev igg and 237 serum samples negative for anti-hev were also assayed for igg anti-hav antibodies by elisa. anti-hev igg was confirmed in 23/996 samples, resulting in a seroprevalence of 2.3% for hev infection, similar to previous results obtained in developed countries. no significant association was found between the presence of anti-hev igg antibodies and the sociodemographic variables including gender, age, educational level, rural or urban areas, source of water, and sewer system (p > 0.05). also, no association with seropositivity for anti-hav igg antibodies was observed (p > 0.05). although this study revealed a low seroprevalence of hev infection in the population evaluated, the results showed that this virus is circulating among the population from londrina, south brazil, and point out the need of further studies to define the clinical and epidemiological importance of hev infection and to identify additional risk factors involved in the epidemiology and pathogenesis of this infection in this population.
Prevalência de tripanossomíase americana, sífilis, toxoplasmose, rubéola, hepatite B, hepatite C e da infec o pelo vírus da imunodeficiência humana, avaliada por intermédio de testes sorológicos, em gestantes atendidas no período de 1996 a 1998 no Hospital Universitário Regional Norte do Paraná (Universidade Estadual de Londrina, Paraná, Brasil)
Reiche Edna Maria Vissoci,Morimoto Helena Kaminami,Farias Grazieli Nogueira,Hisatsugu Kátia Regina
Revista da Sociedade Brasileira de Medicina Tropical , 2000,
Abstract: Com o objetivo de determinar a soroprevalência de tripanossomíase americana, sífilis, toxoplasmose, rubéola, hepatite B, hepatite C e infec o pelo vírus da imunodeficiência humana em gestantes atendidas no Hospital Universitário Regional Norte do Paraná, da Universidade Estadual de Londrina, Paraná, foi realizado estudo retrospectivo dos resultados dos testes sorológicos efetuados no período de junho de 1996 a junho de 1998. As taxas de positividade encontradas foram: 0,9% para tripanossomíase americana, 1,6% para sífilis, 67% (IgG) e 1,8% (IgM) para toxoplasmose, 89% (IgG) e 1,2% (IgM) para rubéola, 0,8% para hepatite B (AgHBs), 0,8% para hepatite C e 0,6% para infec o pelo vírus da imunodeficiência humana. Observou-se associa o entre o aumento da soroprevalência de tripanossomíase americana com a idade das gestantes (p = 0,006). Os resultados reafirmam a importancia da realiza o destes testes sorológicos no atendimento pré-natal, com a finalidade de realizar o diagnóstico e, eventualmente, adotar medidas para prevenir a transmiss o congênita ou perinatal dessas doen as.
Evaluation of surrogate markers for human immunodeficiency virus infection among blood donors at the blood bank of "Hospital Universitário Regional Norte do Paraná", Londrina, PR, Brazil
Reiche Edna Maria Vissoci,Vogler Ingridt Hildegard,Morimoto Helena Kaminami,Bortoliero André Luis
Revista do Instituto de Medicina Tropical de S?o Paulo , 2003,
Abstract: This study evaluated the usefulness of the anti-HBc, hepatitis C virus antibodies (anti-HCV), human T cell lymphotropic virus I and II antibodies (anti-HTLV I/II), serologic tests for syphilis, and surface antigen of hepatitis B virus (HBsAg) as surrogate markers for the risk for HIV infection in 80,284 serum samples from blood donors from the Blood Bank of "Hospital Universitário Regional Norte do Paraná", Londrina, Paraná State, Brazil, analyzed from July 1994 to April 2001. Among 39 blood donors with positive serology for HIV, 12 (30.8%) were anti-HBc positive, 10 (25.6%) for anti-HCV, 1 (2.6%) for anti-HTLV I/I, 1 (2.6%) was positive for syphilis, and 1 (2.6%) for HBsAg. Among the donors with negative serology for HIV, these markers were detected in 8,407 (10.5%), 441 (0.5%), 189 (0.2%), 464 (0.6%), and 473 (0.6%) samples, respectively. The difference was statistically significant (p < 0.001) for anti-HBc and anti-HCV. Although the predictive positive value for these surrogate markers were low for HIV infection, the results confirmed the anti-HBc and anti-HCV as useful surrogate markers for HIV infection thus reinforcing the maintenance of them in the screening for blood donors contributing to the prevention of the small number of cases in which HIV is still transmitted by transfusion.
Immunogenicity of three recombinant hepatitis B vaccines administered to students in three doses containing half the antigen amount routinely used for adult vaccination
Baldy José Luís da Silveira,Lima Gerson Zanetta de,Morimoto Helena Kaminami,Reiche Edna Maria Vissoci
Revista do Instituto de Medicina Tropical de S?o Paulo , 2004,
Abstract: We evaluated the immunogenicity of three recombinant hepatitis B vaccines, one Brazilian (Butang, Instituto Butantan) and two Korean vaccines (Euvax-B, LG Chemical Ltd. and Hepavax-Gene, Greencross Vaccine Corp.), administered intramuscularly to students aged 17 to 19 years in three 10-μg doses (corresponding to half the amount of antigen routinely used for adult vaccination) at intervals of one month between the first and second dose, and of four months between the second and third dose. A total of 316 students non-reactive for any serological marker of hepatitis B virus infection were vaccinated: 77 (24.4%) with the Butang vaccine, 71 (22.5%) with Euvax-B, 85 (26.9%) with Hepavax-Gene and, for comparison, 83 (26.2%) with Engerix-B (GlaxoSmithKline), whose efficacy in young adults at the dose used here has been confirmed in previous studies. Similar seroconversion rates (anti-HBs > 10 mIU/mL about one month after application of the third dose) were obtained for the Butang, Euvax-B, Hepavax-Gene and Engerix-B vaccines (96.2%, 98.6%, 96.5% and 97.6%, respectively). The frequency of good responders (anti-HBs > 100 mIU/mL) was also similar among students receiving the four vaccines (85.8%, 91.6%, 89.4% and 89.2%, respectively). The geometric mean titers (GMT) of anti-HBs about one month after the third dose obtained with these vaccines were 727.78 ± 6.46 mIU/mL, 2009.09 ± 7.16 mIU/mL, 1729.82 ± 8.85 mIU/mL and 2070.14 ± 11.69 mIU/mL, respectively. The GMT of anti-HBs induced by the Euvax-B and Engerix-B vaccines were higher than those obtained with the Butang vaccine (p < 0.05); this difference was not significant when comparing the other vaccines two-by-two. No spontaneous adverse effects attributable to the application of any dose of the four vaccines were reported.
Difficulties in the diagnosis of HTLV-2 infection in HIV/AIDS patients from Brazil: comparative performances of serologic and molecular assays, and detection of HTLV-2b subtype
Morimoto, Helena Kaminami;Morimoto, Arilson Akira;Reiche, Edna Maria Vissoci;Ueda, Luiz Toshio;Matsuo, Tiemi;Reiche, Fernando Vissoci;Caterino-de-Araujo, Adele;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2007, DOI: 10.1590/S0036-46652007000400006
Abstract: the current diagnosis of human t-lymphotropic virus type-2 (htlv-2) infection is based on the search of specific antibodies; nevertheless, several studies conducted in brazil pointed deficiencies of the commercially available kits in detecting htlv-2, mostly in hiv/aids patients. this study searched for the presence of htlv-1 and -2 in 758 hiv/aids patients from londrina, paraná, brazil. serum samples were screened for htlv-1/2 antibodies using two eia kits (vironostika and murex), and confirmed by wb (htlv blot 2.4, genelabs). the results obtained by eia disclosed 49 (6.5%) reactive sera: 43 positive by both eia kits, and six with discordant results. wb confirmed htlv-1 infection in seven samples (0.9%) and htlv-2 in 21 sera (2.8%). negative and indeterminate results were detected in four (0.5%) and 16 (2.1%) sera, respectively. blood from 47 out of 49 htlv seroreactive patients were collected and analyzed for the presence of env, ltr and tax genomic segments of htlvs by pcr. pcr confirmed six cases of htlv-1 and 37 cases of htlv-2 infection (14 out of 16 that were found to be wb indeterminate). restriction analysis of the env pcr products of htlv-2 disclosed 36 isolates of htlv-2a/c subtype, and one of htlv-2b subtype. these results emphasize the need of improving serologic tests for detecting truly htlv-2 infected patients from brazil, and confirm the presence of htlv-2b subtype in the south of this country.
Prevalência de tripanossomíase americana, sífilis, toxoplasmose, rubéola, hepatite B, hepatite C e da infec??o pelo vírus da imunodeficiência humana, avaliada por intermédio de testes sorológicos, em gestantes atendidas no período de 1996 a 1998 no Hospital Universitário Regional Norte do Paraná (Universidade Estadual de Londrina, Paraná, Brasil)
Reiche, Edna Maria Vissoci;Morimoto, Helena Kaminami;Farias, Grazieli Nogueira;Hisatsugu, Kátia Regina;Geller, Lilian;Gomes, Ana Carolina Lima Frade;Inoue, Helena Yurika;Rodrigues, Gisele;Matsuo, Tiemi;
Revista da Sociedade Brasileira de Medicina Tropical , 2000, DOI: 10.1590/S0037-86822000000600002
Abstract: in order to evaluate the seroprevalence of the american trypanosomiasis, syphilis, toxoplasmosis, rubella, hepatitis b infection, hepatitis c infection and human immunodeficiency virus infection among pregnant women attended at the hospital universitário regional norte do paraná, londrina state university, paraná, a retrospective study of the serologic results performed in the prenatal routine during the period of june 1996 to june 1998 was carried out. the rates of seropositivity were as follows: american trypanosomiasis = 0.9%, syphilis = 1.6%, toxoplasmosis = 67% (igg) and 1.8% (igm), rubella = 89% (igg) and 1.2% (igm), hepatitis b surface antigen = 0.8%, hepatitis c virus = 0.8% and human immunodeficiency virus infection = 0.6%. an association between the increase in the seroprevalence of chagas' disease and patient age was detected (p=0.006). the results underscore the importance of the serological tests in perinatal care, to prevent both the congenital and perinatally transmitted forms of theses infectious diseases.
Immunogenicity of three recombinant hepatitis B vaccines administered to students in three doses containing half the antigen amount routinely used for adult vaccination
Baldy, José Luís da Silveira;Lima, Gerson Zanetta de;Morimoto, Helena Kaminami;Reiche, Edna Maria Vissoci;Matsuo, Tiemi;Mattos, Edlivia Dias de;Sudan, Luci Cristina Pulga;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2004, DOI: 10.1590/S0036-46652004000200009
Abstract: we evaluated the immunogenicity of three recombinant hepatitis b vaccines, one brazilian (butang, instituto butantan) and two korean vaccines (euvax-b, lg chemical ltd. and hepavax-gene, greencross vaccine corp.), administered intramuscularly to students aged 17 to 19 years in three 10-μg doses (corresponding to half the amount of antigen routinely used for adult vaccination) at intervals of one month between the first and second dose, and of four months between the second and third dose. a total of 316 students non-reactive for any serological marker of hepatitis b virus infection were vaccinated: 77 (24.4%) with the butang vaccine, 71 (22.5%) with euvax-b, 85 (26.9%) with hepavax-gene and, for comparison, 83 (26.2%) with engerix-b (glaxosmithkline), whose efficacy in young adults at the dose used here has been confirmed in previous studies. similar seroconversion rates (anti-hbs > 10 miu/ml about one month after application of the third dose) were obtained for the butang, euvax-b, hepavax-gene and engerix-b vaccines (96.2%, 98.6%, 96.5% and 97.6%, respectively). the frequency of good responders (anti-hbs > 100 miu/ml) was also similar among students receiving the four vaccines (85.8%, 91.6%, 89.4% and 89.2%, respectively). the geometric mean titers (gmt) of anti-hbs about one month after the third dose obtained with these vaccines were 727.78 ± 6.46 miu/ml, 2009.09 ± 7.16 miu/ml, 1729.82 ± 8.85 miu/ml and 2070.14 ± 11.69 miu/ml, respectively. the gmt of anti-hbs induced by the euvax-b and engerix-b vaccines were higher than those obtained with the butang vaccine (p < 0.05); this difference was not significant when comparing the other vaccines two-by-two. no spontaneous adverse effects attributable to the application of any dose of the four vaccines were reported.
Serological, epidemiological and molecular aspects of hepatitis C virus infection in a population from Londrina, PR, Brazil, 2001-2002
Vogler, Ingridt Hildegard;Nishiya, Anna;Morimoto, Helena Kaminami;Reiche, Edna Maria Vissoci;Bortoliero, André Luiz;Matsuo, Tiemi;Sabino, Ester Cerdeira;Vaz, Adelaide Jose;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2004, DOI: 10.1590/S0036-46652004000600002
Abstract: serological, epidemiological and molecular aspects of hepatitis c virus (hcv) infection were evaluated in 183 subjects from londrina, paraná, brazil, and adjacent areas. serum samples which tested anti-hcv positive by microparticle enzyme immunoassay (meia) obtained from eight patients with chronic hepatitis c, 48 blood donors, and 127 patients infected with the human immunodeficiency virus (hiv) were submitted to another enzyme immunoassay (elisa) and to the polymerase chain reaction (pcr). about 78.7% of samples were also reactive by elisa, with the greater proportion (70.8%) of discordant results verified among blood donors. a similar finding was observed for hcv-rna detection by pcr, with 111/165 (67.3%) positive samples, with higher rates among hiv-positive subjects and patients with chronic hepatitis than among blood donors. sixty-one pcr-positive samples were submitted to hcv genotyping, with 77.1, 21.3 and 1.6% of the samples identified as types 1, 3 and 2, respectively. finally, analysis of some risk factors associated with hcv infection showed that intravenous drug use was the most common risk factor among hiv/hcv co-infected patients, while blood transfusion was the most important risk factor in the group without hiv infection. the present study contributed to the knowledge regarding risk factors associated with hcv infection and the distribution of hcv genotypes in the population evaluated.
Seropositivity for anti-Trypanosoma cruzi antibodies among blood donors of the "Hospital Universitário Regional do Norte do Paraná", Londrina, Brazil
Reiche, Edna Maria Vissoci;Inouye, Marta Mutsumi Zaha;Pontello, Rubens;Morimoto, Helena Kaminami;Jankevicius, Shiduca Itow;Matsuo, Tiemi;Jankevicius, José Vitor;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1996, DOI: 10.1590/S0036-46651996000300014
Abstract: the most frequent form of acquisition of chagas' disease in endemic areas was the transmission through the feces of contaminated triatominae. however, special attention should be paid in urban areas to transmission by blood transfusion, justifying the compulsory screening of blood donors. early investigations at blood banks in the town of londrina, brazil, demonstrated that the seroprevalence of anti-trypanosoma cruzi antibodies among blood donors was approximately 7.0% in the fifties9,34. further studies demonstrated pratically the same seroprevalence until the eighties4,32,41. in an attempt to obtain data about the real dimension of the seropositivity for anti-trypasonoma cruzi antibodies in the region, the authors carried out a large-scale study on 45,774 serum samples from blood donors of the hemocentro of hospital universitário regional do norte do paraná (hurnp), universidade estadual de londrina. the immunological tests were done at the division of clinical immunology of hurnp from may 1990 to december 1994. the serum samples were studied by the indirect hemagglutination assay (iha, using kits commercially obtained from ebram) and by indirect immunofluorescence (ifi, using kits from lio serum) with anti-human igg conjugate (laborclin). the results demonstrated that 643 serum samples were positive in both assay corresponding to a seroprevalence of 1.4%, i.e., a significant decrease in anti-trypanosoma cruzi antibodies in the region in comparison with the previously mentioned rates. data correlating sex and age of seropositive blood donors are presented, as well as the possible factors that may have contributed to the results observed.
Evaluation of surrogate markers for human immunodeficiency virus infection among blood donors at the blood bank of "Hospital Universitário Regional Norte do Paraná", Londrina, PR, Brazil
Reiche, Edna Maria Vissoci;Vogler, Ingridt Hildegard;Morimoto, Helena Kaminami;Bortoliero, André Luis;Matsuo, Tiemi;Yuahasi, Kátia Kioko;Cancian, Sanderson Júnior;Koguichi, Roberto Setsuo;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2003, DOI: 10.1590/S0036-46652003000100005
Abstract: this study evaluated the usefulness of the anti-hbc, hepatitis c virus antibodies (anti-hcv), human t cell lymphotropic virus i and ii antibodies (anti-htlv i/ii), serologic tests for syphilis, and surface antigen of hepatitis b virus (hbsag) as surrogate markers for the risk for hiv infection in 80,284 serum samples from blood donors from the blood bank of "hospital universitário regional norte do paraná", londrina, paraná state, brazil, analyzed from july 1994 to april 2001. among 39 blood donors with positive serology for hiv, 12 (30.8%) were anti-hbc positive, 10 (25.6%) for anti-hcv, 1 (2.6%) for anti-htlv i/i, 1 (2.6%) was positive for syphilis, and 1 (2.6%) for hbsag. among the donors with negative serology for hiv, these markers were detected in 8,407 (10.5%), 441 (0.5%), 189 (0.2%), 464 (0.6%), and 473 (0.6%) samples, respectively. the difference was statistically significant (p < 0.001) for anti-hbc and anti-hcv. although the predictive positive value for these surrogate markers were low for hiv infection, the results confirmed the anti-hbc and anti-hcv as useful surrogate markers for hiv infection thus reinforcing the maintenance of them in the screening for blood donors contributing to the prevention of the small number of cases in which hiv is still transmitted by transfusion.
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