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Search Results: 1 - 10 of 27595 matches for " Hea Jin Ryu "
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Tumor necrosis factor-α-mediated threonine 435 phosphorylation of p65 nuclear factor-κB subunit in endothelial cells induces vasogenic edema and neutrophil infiltration in the rat piriform cortex following status epilepticus
Ji-Eun Kim, Hea Jin Ryu, Soo Young Choi, Tae-Cheon Kang
Journal of Neuroinflammation , 2012, DOI: 10.1186/1742-2094-9-6
Abstract: SE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.Following SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.These findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss.Status epilepticus (SE) is a medical emergency with significant mortality [1]. SE has been defined as continuous seizure activity, which causes neuronal cell death, epileptogenesis and learning impairment [2,3]. Some brain regions vulnerable to SE play a role in the generation and propagation of paroxysmal activity in experimental epilepsy models. The piriform cortex (PC) is one of the most susceptible brain regions to seizure-induced damage in the kainate, pilocarpine and other models of temporal lobe epilepsy (TLE) [4-6]. Pilocarpine, a cholinergic agonist, induces SE in rodents. This pilocarpine-induced SE, similar to human TLE, shows massive neuronal loss in the hippocampus followed by glial proliferation. This neuronal damage in the pilocarpine model is not restricted to the hippocampus, but often extends to extrahippocampal limbic struc
P2X7 receptor activation ameliorates CA3 neuronal damage via a tumor necrosis factor-α-mediated pathway in the rat hippocampus following status epilepticus
Ji-Eun Kim, Hea Jin Ryu, Tae-Cheon Kang
Journal of Neuroinflammation , 2011, DOI: 10.1186/1742-2094-8-62
Abstract: SE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, 2',3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP), adenosine 5'-triphosphate-2',3'-dialdehyde (OxATP), A-438079, or A-740003 prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunit phosphorylations.Following SE, P2X7 receptor agonist (BzATP) infusion increased TNF-α immunoreactivity in dentate granule cells as compared with that in saline-infused animals. In addition, TNF-α immunoreactivity was readily apparent in the mossy fibers, while TNF-α immunoreactivity in CA1-3 pyramidal cells was unaltered. However, P2X7 receptor antagonist (OxATP-, A-438079, and A-740003) infusion reduced SE-induced TNF-α expression in dentate granule cells. In the CA3 region, BzATP infusion attenuated SE-induced neuronal damage, accompanied by enhancement of p65-Ser276 and p65-Ser311 NF-κB subunit phosphorylations. In contrast, OxATP-, A-438079, and A-740003 infusions increased SE-induced neuronal death. Soluble TNF p55 receptor (sTNFp55R), and cotreatment with BzATP and sTNFp55R infusion also increased SE-induced neuronal damage in CA3 region. However, OxATP-, sTNFp55R or BzATP+sTNFp55R infusions could not exacerbate SE-induced neuronal damages in the dentate gyrus and the CA1 region, as compared to BzATP infusion.These findings suggest that TNF-α induction by P2X7 receptor activation may ameliorate SE-induced CA3 neuronal damage via enhancing NF-κB p65-Ser276 and p65-Ser311 phosphorylations.Status epilepticus (SE) is a medical emergency with significant mortality [1]. SE has been defined as continuous seizure activity, which causes neuronal cell death [2,3], epileptogenesis [3] and learning impairment [4]. Cytokines are critical mediators of specific inflammatory responses and immune reactions in the brain [5]. Tumor necrosis factor-α (TNF-α) is a 17-kDa protein that is mainly produced by activated macrophag
Status Epilepticus Induces Vasogenic Edema via Tumor Necrosis Factor-α/ Endothelin-1-Mediated Two Different Pathways
Ji-Eun Kim, Hea Jin Ryu, Tae-Cheon Kang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074458
Abstract: Status epilepticus (SE) induces vasogenic edema in the piriform cortex with disruptions of the blood-brain barrier (BBB). However, the mechanisms of vasogenic edema formation following SE are still unknown. Here we investigated the endothelin B (ETB) receptor-mediated pathway of SE-induced vasogenic edema. Following SE, the release of tumor necrosis factor-α (TNF-α) stimulated endothelin-1 (ET-1) release and expression in neurons and endothelial cells. In addition, TNF-α-induced ET-1 increased BBB permeability via ETB receptor-mediated endothelial nitric oxide synthase (eNOS) activation in endothelial cells. ETB receptor activation also increased intracellular reactive oxygen species by NADPH oxidase production in astrocytes. These findings suggest that SE results in BBB dysfunctions via endothelial-astroglial interactions through the TNF-α-ET-1-eNOS/NADPH oxidase pathway, and that these ETB receptor-mediated interactions may be an effective therapeutic strategy for vasogenic edema in various neurological diseases.
Effects of Carbon Dioxide Treatment and Modified Atmosphere Packaging on the Quality of Long Distance Transporting “Maehyang” Strawberry  [PDF]
Hyun Jin Choi, Yeong Seuk Bae, Jung Soo Lee, Me Hea Park, Ji Gang Kim
Agricultural Sciences (AS) , 2016, DOI: 10.4236/as.2016.712074
Abstract: This study was conducted to investigate the effects of carbon dioxide (CO2) and modified atmosphere (MA) packaging on the quality of strawberry during long distance transportation. “Maehyang” strawberries (Fragaria x ananassa Duch.) with red color on 70% of the fruit surface were harvested in Gyeongnam province, Korea. The samples were placed in gas-tight chamber with 30% CO2 concentration for 3 hours at 3°C. Strawberry samples were then packaged with modified atmosphere-modified humidity (MA/MH) packaging film. Samples treated with CO2 alone and combined CO2 with MA packaging were stored for one day at 1°C, transported for 10 days at 1°C, and distributed for 3 days at 4°C. Carbon dioxide alone or combination with MA packaging was effective in maintaining quality of “Maehyang” strawberries. Carbon dioxide treatment significantly increased firmness and reduced softening index and decay rate during 14 day transportation and distribution. Samples treated with CO2 + MA had higher overall score with low softening index and weight loss after 14 days of transportation and low temperature distribution compared to CO2 treatment only. The results indicated that a short term application of CO2 or combination treatment of CO2 and MA could be good postharvest handling for maintaining freshness of “Maehyang” strawberries during long distance vessel export.
P2X7 receptor regulates leukocyte infiltrations in rat frontoparietal cortex following status epilepticus
Ji-Eun Kim, Hea Ryu, Seong-Il Yeo, Tae-Cheon Kang
Journal of Neuroinflammation , 2010, DOI: 10.1186/1742-2094-7-65
Abstract: Status epilepticus (SE) was induced by pilocarpine in rats that were intracerebroventricularly infused with either saline, 2',3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP), adenosine 5'-triphosphate-2',3'-dialdehyde (OxATP), or IL-1Ra (interleukin 1 receptor antagonist) prior to SE induction. Thereafter, we performed immunohistochemical studies for myeloperoxidase (MPO), CD68, interleukin-1β (IL-1β), monocyte chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2).In saline-infused animals, neutrophils and monocytes were observed in frontoparietal cortex (FPC) at 1 day and 2 days after SE, respectively. In BzATP-infused animals, infiltrations of neutrophils and monocytes into the FPC were detected at 12 hr and 1 day after SE, respectively. In OxATP-infused animals, neutrophils and monocytes infiltrated into the FPC at 1 day and 2 days after SE, respectively. However, the numbers of both classes of leukocytes were significantly lower than those observed in the saline-infused group. In piriform cortex (PC), massive leukocyte infiltration was detected in layers III/IV of saline-infused animals at 1-4 days after induction of SE. BzATP or OxATP infusion did not affect neutrophil infiltration in the PC. In addition, P2X7 receptor-mediated MCP-1 (released from microglia)/MIP-2 (released from astrocytes) regulation was related to SE-induced leukocyte infiltration in an IL-1β-independent manner.Our findings suggest that selective regulation of P2X7 receptor-mediated neutrophil infiltration may provide new therapeutic approaches to SE or epilepsy.Epilepsy is a chronic condition characterized by the presence of spontaneous episodes of abnormal excessive neuronal discharges that result in specific patterns of neuron loss in various brain regions, particularly in the hippocampus [1,2]. Recent reports have emphasized that chronic epilepsy is a prolonged inflammatory condition, and that epileptic activity rapidly increases synthesis and release of v
Ji Hea Woo,Jooyeon Ko,Young Eun Choi,Her Jin Gang
Biology of Sport , 2013,
Abstract: The axe kick, in Olympic style taekwondo, has been identified as the most popular scoring technique aimed to the head during full contact competition. The first purpose of this study was to identify and investigate design issues with the current World Taekwondo Federation approved chest protector. A secondary purpose was to develop a novel chest protector addressing the identified design issues and to conduct a biomechanical analysis. Fifteen male elite Taekwondo players were selected to perform three different styles of the axe kick, i.e., front, in-out, and out-in axe kick five times each for a total of 45 kicks. Two-way repeated measures ANOVA showed significant differences between the novel and existing chest protector conditions for vertical height of the toe, downward kicking foot speed, hip flexion angle and ipsilateral shoulder flexion extension range of motion (ROM) (p<0.05). There were no significant differences between the control condition (no chest protector) and the novel chest protector condition for these variables (p>0.05). These results indicate that the novel chest protector interferes less with both the lower and upper limbs during the performance of the axe kick and provides a more natural, free-moving alternative to the current equipment used.
Effects of creatine and β-guanidinopropionic acid and alterations in creatine transporter and creatine kinases expression in acute seizure and chronic epilepsy models
Dae Kim, Seong-Il Yeo, Hea Ryu, Ji-Eun Kim, Hong-Ki Song, Oh-Shin Kwon, Soo Choi, Tae-Cheon Kang
BMC Neuroscience , 2010, DOI: 10.1186/1471-2202-11-141
Abstract: Two hr after pilocarpine (PILO)-seizure induction, ubiquitous mitochondrial CK (uMtCK) immunoreactivity was unaltered as compared to control level. However, brain-type cytoplasm CK (BCK) immunoreactivity was decreased to 70% of control level. CRT immunoreactivity was decreased to 60% of control level. Following Cr or Tat-CK treatment, uMtCK or CRT immunoreactivity was unaffected, while BCK immunoreactivity in Cr treated group was increased to 3.6-fold of control levels. β-Guanidinopropionic acid (GPA, a competitive CRT inhibitor) reduced BCK and CRT expression. In addition, Cr and tat-BCK treatment delayed the beginning of seizure activity after PILO injection. However, GPA treatment induced spontaneous seizure activity without PILO treatment. In chronic epilepsy rats, both uMtCK and CRT immunoreactivities were reduced in the hippocampus. In contrast, BCK immunoreactivity was similar to that observed in control animals. Cr-, GPA and tat-BCK treatment could not change EEG.Cr/CK circuit may play an important role in sustaining or exacerbating acute seizure activity, but not chronic epileptic discharge.Maintenance of energy homeostasis in the brain requires a distinct molecular circuitry which provides tight coupling between energy consumption and production during the performance of sensory, motor and cognitive processes [1,2]. It is generally assumed that most energy required in the nervous system is provided in the form of adenosine triphosphate (ATP) by mitochondria [3]. ATP production by glycolysis in glia [4] and neurons has been recognized as an alternative source of energy [5,6]. Furthermore, local ATP/ADP ratios and proper distribution of metabolic energy are maintained by catalyzed exchange of high-energy phosphoryls between γ-ATP and phosphocreatine (PCr) or β-adenosine diphosphate (β-ADP) [7,8].The brain is a main target in infants with creatine (Cr)-deficiency syndrome; the patients exhibit delayed psychomotor development, hypotonia, seizure and myelinatio
The Efficacy and Safety of Succinylated Atelocollagen and Adenosine for the Treatment of Periorbital Wrinkles  [PDF]
Dong Jin Ryu, Jin Young Jung, Kee Yang Chung, Hwal Suh, Sang Ho Oh, Ju Hee Lee
Journal of Cosmetics, Dermatological Sciences and Applications (JCDSA) , 2013, DOI: 10.4236/jcdsa.2013.33036

The degradation of structural collagen contributes to the characteristic appearance of wrinkles. The anti-wrinkle effects of a variety of substances have been studied, but the potential anti-wrinkle effects of topical applications of collagen for periorbital wrinkles have not been investigated. To evaluate the effects of topical application of succinylated atelocollagen on periorbital wrinkles and to compare the results of treatment with adenosine, a clinical study on Korean participants was carried out. Each participant’s right or left periorbital area was treated with either a solution containing succinylated atelocollagen and adenosine or a solution containing only succinylated atelocollagen for two months. A placebo solution was applied to the opposite periorbital area of each patient’s face for two months. Based on objective and subjective measurements of clinical improvement, the assessment scores for treated sites were statistically significantly higher than scores for placebo sites after two months of treatment. Analysis of silicone casts of periorbital wrinkles demonstrated partial effects of succinylated atelocollagen on periorbital wrinkles. However, we did not observe any effects of adenosine on periorbital wrinkles. Succinylated atelocollagen may be an effective treatment option for periorbital wrinkles, but further studies including a longer treatment period and larger subject group are needed to verify these results.

Lactic acid bacteria affect serum cholesterol levels, harmful fecal enzyme activity, and fecal water content
Do Lee, Seok Jang, Eun Baek, Mi Kim, Kyung Lee, Hea Shin, Myung Chung, Jin Kim, Kang Lee, Nam Ha
Lipids in Health and Disease , 2009, DOI: 10.1186/1476-511x-8-21
Abstract: In vitro culture experiments were performed to evaluate the ability of Bifidobacterium spp. isolated from healthy Koreans (20~30 years old) to reduce cholesterol-levels in MRS broth containing polyoxyethanylcholesterol sebacate. Animal experiments were performed to investigate the effects on lowering cholesterol, inhibiting harmful enzyme activities, and controlling fecal water content. For animal studies, 0.2 ml of the selected strain cultures (108~109 CFU/ml) were orally administered to SD rats (fed a high-cholesterol diet) every day for 2 weeks.B. longum SPM1207 reduced serum total cholesterol and LDL levels significantly (p < 0.05), and slightly increased serum HDL. B. longum SPM1207 also increased fecal LAB levels and fecal water content, and reduced body weight and harmful intestinal enzyme activities.Daily consumption of B. longum SPM1207 can help in managing mild to moderate hypercholesterolemia, with potential to improve human health by helping to prevent colon cancer and constipation.Probiotic bacteria have multiple potential health effects, including blocking gastroenteric pathogens [1-4], neutralizing food mutagens produced in the colon [1,5-10], enhancing the immune response [6,9,11-14], lowering serum cholesterol, and stopping intestinal dysfunction [15-21]. In general, probiotic bacteria must colonize the gastrointestinal tract (GIT) of the host, have acid- and bile salt-tolerance, and block putrefactive bacteria in the GIT. Lactic acid bacteria (LAB), especially Lactobacillus spp. and Bifidobacterium spp. are important GIT residents and are used as probiotic strains to improve health [22-24]. Lactobacillus and Bifidobacterium have been used in fermented foods for several centuries without adverse effects [25,26] and are classified as Generally Recognized as Safe (GRAS) because of their long history of safe use, particularly in dairy foods [27,28].Here, we evaluated the ability of Bifidobacteria spp. isolated from healthy Koreans (20~30 years old) to
Progesterone produces antinociceptive and neuroprotective effects in rats with microinjected lysophosphatidic acid in the trigeminal nerve root
Min Kim, Hea Shin, Kyoung Won, Kui Yang, Jin Ju, Yoon Park, Jae Park, Yong Bae, Dong Ahn
Molecular Pain , 2012, DOI: 10.1186/1744-8069-8-16
Abstract: Under anesthesia, the Sprague-Dawley rats were mounted onto a stereotaxic frame and 3 μL of lysophosphatidic acid (LPA, 1 nmol) was injected into the trigeminal nerve root to produce demyelination. This treatment decreased the air-puff thresholds, persisted until postoperative day 130, and then returned to the preoperative levels 160 days after LPA injection. The LPA-treated rats also showed a significant hyper-responsiveness to pin-prick stimulation. We further investigated the antinociceptive and neuroprotective effects of progesterone in rats undergoing demyelination of the trigeminal nerve root. Progesterone (8, 16 mg/kg/day) was administered subcutaneously, beginning on the operative day, for five consecutive days in the LPA-treated rats. Treatment with progesterone produced significant early anti-allodynic effects and delayed prolonged anti-allodynic effects. The expression of protein zero (P0) and peripheral myelin protein 22 (PMP22) were significantly down-regulated in the trigeminal nerve root on postoperative day 5 following LPA injection. This down-regulation of the P0 and PMP22 levels was blocked by progesterone treatment.These results suggest that progesterone produces antinociceptive effects through neuroprotective action in animals with LPA-induced trigeminal neuropathic pain. Moreover, progesterone has potential utility as a novel therapy for trigeminal neuropathic pain relief at an appropriate managed dose and is therefore a possible future treatment strategy for improving the recovery from injury.Progesterone is a female gonadal steroid hormone synthesized in the ovary that exerts a wide range of actions against its target tissues including the uterus, mammary glands and brain. There is however abundant evidence now that progesterone has functions that go beyond its role as a female sex hormone. Exogenous progesterone and its derivates have been shown to be a successful treatment for rat models of traumatic brain injury and stroke [1-4], and periph
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