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OALib Journal期刊

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Evodiamine Inhibits the Proliferation of BGC-823 and SGC-7901 Cells by Inducing Cell Cycle Arrest and Apoptosis in Gastric Cancer  [PDF]
Hanni Zhang, Yunliang Guo, Keli Ge, Yanan Wang
Open Access Library Journal (OALib Journal) , 2019, DOI: 10.4236/oalib.1105217
Abstract:
Gastric cancer represents a major cause of cancer-related death worldwide. Although various tactics and anti-tumor drugs have been used to improve curative effects, five-year survival rate of lung cancer patients remains poor. Evodiamine, a sophora alkaloid, has been demonstrated to exert antitumor effects on many types of cancer. However, the molecular mechanism of evodiamine against gastric cancer has not been clearly elucidated. In this study, we investigated the anti-tumor activity and the underlying mechanisms of EVO on gastric cancer cells, and found that it significantly inhibited the proliferation of BGC-823 and SGC-7901 cells by inducing cell cycle arrest at G2/M phase and cell apoptosis in a dose- and time-dependent manner. Its molecular mechanism may be that it reduces the expression of cell cycle- promoting protein Cdc25C and promotes the expression of cell cycle inhibitor p53, as well as prompts the activity of caspases pathways, such as the expression level of cleaved caspase-3 and cleaved caspase-8; cleaved caspase-9 and cleaved PARP-1 are up-regulated, treated with EVO (10 μM) at different points in time (0, 3, 6, 9, 12, 24 h). Collectively, our data demonstrated that EVO was a potential anti-tumor agent against gastric cancer.
Anti-Tumor Effect and Mechanism of Parthenolide in Gastric Cancer Cellline BGC-823  [PDF]
Wenhao Zhu, Hanni Zhang, Yunliang Guo, Keli Ge, Yonghong Zhou
Open Access Library Journal (OALib Journal) , 2019, DOI: 10.4236/oalib.1105271
Abstract:
Objective: To study the anti-tumor effect and mechanism of parthenolide in gastric cancer cell BGC-823. Methods: The cck8 assay was used to detect the changes of BGC-823 cells viability after treatment with different con-centrations of parthenolide at different time points. The proliferation ability of BGC-823 cells was detected by clone formation assay. And the cell cycle and apoptosis were measured by flow cytometry. Meanwhile, to detect the different in intracellular ROS production levels, the fluorescence assay was used. And with the help of western blotting, cell cycle- and apoptosis-related protein expression can be detected. Results: Parthenolide could inhibit the viability of BGC-823 cells in a dose- and time-dependent manner (P < 0.01). In BGC-823 cells exposed to parthenolide, the apoptosis rate was found significantly increased (P < 0.01), and the protein expression of cleaved-caspase3, cleaved-caspase8, and cleaved-caspase9 significantly increased (P < 0.01); the cell cycle was arrested at G1phase (P < 0.01); the protein levels of CyclinD1 and CyclinE1 decreased (P < 0.01), and the expression of P53 and P21 protein increased (P < 0.01); massive intracellular ROS generation was found (P < 0.01). Furthermore, the nuclear protein levels of c-Myc, E2F1, and NF-κB and the protein level of phosphorylated STAT3 decreased in BGC-823 cells exposed to parthenolide (P < 0.01). Conclusion: Parthenolide may inhibit the proliferation of BGC-823 cells and induce G1-phase cell cycle arrest and apoptosis via inhibiting STAT3-c-Myc-E2F1 axis.
Primary Papillary Mucinous Adenocarcinoma of the Ureter Mimicking Genitourinary Tuberculosis
Hanni Gulwani,Aruna Jain
Pathology Research International , 2010, DOI: 10.4061/2010/738407
Abstract: Primary adenocarcinomas of the renal pelvis and ureter are rare and account for less than 1% of all malignancies at this site. We report a case of primary papillary mucinous adenocarcinoma of the ureter that clinically mimicked genitourinary tuberculosis. Early diagnosis is important for the better outcome. 1. Introduction Primary adenocarcinomas of the renal pelvis and ureter are rare and have been reported mainly in the form of single cases or small series. Most of these arise as a result of glandular metaplasia. The predisposing factors include nephrolithiasis and repeated infections. Associated malignancies are high grade and widely invasive at presentation [1]. We hereby present an unusual case report of primary papillary mucinous adenocarcinoma of the ureter that clinically mimicked genitourinary tuberculosis. 2. Case Report A 54-year-old male patient presented to the Urology Department of Bhopal Memorial Hospital and Research Centre with right flank pain for last two weeks. There were no complaints of hematuria and pyuria. 3. Radiological Investigations Ultrasound examination revealed moderately hydronephrotic right kidney with dilated right ureter. The dilatation involved lower rd of the ureter suggesting stricture. No calculus or mass was seen in the right kidney. Bulb ureterogram revealed complete ureteric block. Renal function tests were deranged. On LUT endoscopy, the right ureteric orifice, urethra, bladder neck, and bladder were within normal limits. The left kidney and ureter were also unremarkable. A clinical diagnosis of genitourinary tuberculosis associated with right-sided hydronephrosis and hydroureter was made. Perioperatively, right ureteric lumen was narrowed and occluded in its lower one third. The wall was mildly edematous. Patient refused to undergo nephrectomy; therefore, the stricture was relieved and ureteric reimplantation was achieved using Boari flap with psoas hitch. Biopsy was taken from right ureteral stricture and sent for histopathological examination. 4. Pathology Findings Routinely stained sections from the ureteric biopsy revealed a papillary adenocarcinoma filling the lumen. The tumor was occupying nearly two thirds of the ureteric mucosa and rest one third of the ureteric mucosa was lined by attenuated transitional epithelium with focal ulceration (Figure 1(a)). The tumor had papillary projections with a central fibrovascular core. The papillae were lined by tall columnar stratified epithelium and several of the epithelial cells contained mucin. In other areas tumor had glandular pattern and the glands were
Gender as Pathology: Disease, Degeneration, and Medical Discourse in Late Nineteenth-century Colombia El género como patología: enfermedad, degeneración y discurso médico a finales del siglo XIX en Colombia
Hanni Jalil Paier
Revista CS , 2012,
Abstract: This article examines how Colombian doctors and public health officials during the lastdecades of the nineteenth century produced a body of knowledge about the health of thenation’s citizens, using the language and authority of science to speak about a society inneed of redemption and medical intervention. In these cases, gender became an essentialcomponent of elite and medical discourses. Medical doctors and hygienists described femaleidentities either as potentially threatening and therefore degenerative to the nation’s moraland economic fabric or as a “civilizing force” through the mobilization of motherhood andthe reification of the Colombian family as a regenerative site. The doctors and governmentofficials here examined expected women to preserve the family as a unit and inculcate thevalues of order, hygiene and efficiency in the private sphere. If elite constructions of “ideal”female identities mobilized women in their primary function as mothers, preoccupations withthe control of “public women” that upset public order or threatened the family unit rhetoricallyemphasized their deviance. In direct contrast to the feminine ideal, the constructionof the feminine other emphasized moral transgression and sexual promiscuity.
Of Structures, Culture and Other Demons: A Review of Late Eighteenth-Century Andean Insurrections Of Structures, Culture and Other Demons: A Review of Late Eighteenth-Century Andean Insurrections
Hanni Jalil Paier
Revista CS , 2011,
Abstract: Este ensayo observa las insurrecciones populares del siglo XVIII no como episodios casuales o aislados, sino como síntomas de un sentimiento generalizado de descontento social e intensificado conflicto. Sentimientos cuya intensidad se incrementó durante la segunda mitad del siglo XVIII y culminó con la Gran Rebelión de 1780-1783 en el sur de los Andes, el levantamiento en Quito de 1765 y la Rebelión de los Comuneros en la Nueva Granada de 1781. Este artículo examina los trabajos que hacen referencia a estas revueltas, reconociendo el hecho que la producción académica de las insurrecciones de finales del siglo XVIII en las colonias americanas forman parte de los amplios debates académicos que abordan las insurrecciones fuera del contexto Andino, e incorpora preguntas promulgadas por los estudiosos de las revueltas campesinas y el conflicto armado en otros campos y otros periodos. This review essay visualizes eighteenth-century popular insurrections not as casual or isolated episodes, but rather as symptomatic expressions of social tensions and heightened conflict; feelings that increased in intensity during the latter half of the century and culminated in the Great Rebellion of 1780-1783 in the Southern Andes, the 1765 Quito uprising and the 1781 Comunero Revolt in Nueva Granada. The article examines journal articles and monographs that address these revolts, acknowledging that academic production on late eighteenth-century insurrections in the Spanish colonies is itself suspended within larger scholarly debates that address insurrections outside the Andean context and incorporate questions raised by scholars of peasant revolts and agrarian conflict in other fields and time periods.
Mucin-producing signet ring cell adenoma of the thyroid
Gulwani Hanni,Chopra P
Indian Journal of Pathology and Microbiology , 2008,
Abstract: Signet ring cell adenoma of the thyroid, though rare, is well documented. This change is chiefly due to intracellular accumulation of thyroglobulin that appears mucinous. Awareness of this entity is important as it may closely simulate a metastatic mucin-secreting signet ring cell carcinoma. Although the mucinous material in signet ring cells has been reported to stain positive with thyroglobulin, in some cases it may not be so. We herein describe a rare case of a 46-year-old man who was hypothyroid and the mass removed from the thyroid showed a mucin-producing signet ring cell adenoma of the thyroid.
Mucinous cystadenocarcinoma: A rare primary malignant tumor of the breast
Gulwani Hanni,Bhalla Sunita
Indian Journal of Pathology and Microbiology , 2010,
Abstract:
Chromatin architecture and gene expression in Escherichia coli
Hanni Willenbrock, David W Ussery
Genome Biology , 2004, DOI: 10.1186/gb-2004-5-12-252
Abstract: Expression of a gene is in a sense a bit like purchasing a new home - the value is strongly dependent on location. This value is context-dependent: it depends on who your neighbors are and also on the larger geographical picture. Two recent studies have analyzed DNA topology and chromatin structure on a genome-wide scale in Escherichia coli [1,2]. Both show that an important factor in determining transcription profiles - when and to what extent a gene is expressed - is the location of the gene within the context of the E. coli K-12 chromosome. Whereas this is old news for those who are interested mainly in eukaryotic chromosomes, it is an important concept that has often been overlooked (in our opinion) in bacterial transcriptomics. In eukaryotes, it is well known that there are two types of chromatin: heterochromatin, which remains condensed for the most part throughout the cell cycle and contains few genes, and euchromatin, which, on the other hand, contains gene-rich regions and in some cases clusters of highly expressed genes.Jeong et al. [1] analyzed similarities in the transcriptional activities of E. coli genes as a function of their position on the chromosome. An autocorrelation function identified three levels of spatial correlations of expressed genes: short-range (7-16 kilobase-pairs, kb), medium-range (approximately 100 kb) and long-range (over 700 kb). Figure 1 shows the gene-expression data obtained by Jeong et al. [1], together with that of Peter et al. [2], mapped onto the circular E. coli chromosome, with four circles (circles 3-6) corresponding to values obtained from the four experiments of Jeong et al. [1]. They took into account the transcription levels of nearly all genes, although only the more highly expressed genes are visible in Figure 1. Most of the genes in E. coli are transcribed around the time of replication [3], and only a small fraction (typically around 10%) of the genes are highly transcribed. These 'clumps' or regions of highly ex
QTL global meta-analysis: are trait determining genes clustered?
Hanni Salih, David L Adelson
BMC Genomics , 2009, DOI: 10.1186/1471-2164-10-184
Abstract: We observed a significant increase in gene density within QTL regions compared to non-QTL regions and/or the entire bovine genome. By grouping QTL from the Bovine QTL Viewer database into 8 categories of non-redundant regions, we have been able to analyze gene density and gene function distribution, based on Gene Ontology (GO) with relation to their location within QTL regions, outside of QTL regions and across the entire bovine genome. We identified a number of GO terms that were significantly over represented within particular QTL categories. Furthermore, select GO terms expected to be associated with the QTL category based on common biological knowledge have also proved to be significantly over represented in QTL regions.Our analysis provides evidence of over represented GO terms in QTL regions. This increased GO term density indicates possible clustering of gene functions within QTL regions of the bovine genome. Genes with similar functions may be grouped in specific locales and could be contributing to QTL traits. Moreover, we have identified over-represented GO terminology that from a biological standpoint, makes sense with respect to QTL category type.Gene density has been shown to vary widely by organism and genomic region and has been measured both in terms of mean interval between genes and genes per mega base pair of DNA [1,2]. It is known that gene density is positively correlated with G+C content [2] and that the heterochromatic regions surrounding centromeres and telomeres have a lower than average gene density [3-5]. In general, measurements of gene density have focused on correlations of gene density with chromosomal structure or base composition [2,6]. However, to our knowledge no one has looked at the correlation of gene density with Quantitative Trait Locus (QTL) density over the genome. Furthermore, gene density on its own is a fairly crude measurement of the functional role of specific genomic domains. It would be more informative to combine thi
Prediction of highly expressed genes in microbes based on chromatin accessibility
Hanni Willenbrock, David W Ussery
BMC Molecular Biology , 2007, DOI: 10.1186/1471-2199-8-11
Abstract: We find that absolute gene expression levels are correlated with the position preference in many microbial genomes. It is postulated that in these regions, the DNA may be more accessible to the transcriptional machinery. Moreover, ribosomal proteins and ribosomal RNA are encoded by DNA having significantly lower position preference values than other genes in fast-replicating microbes.This insight into DNA structure-dependent gene expression in microbes may be exploited for predicting the expression of non-translated genes such as non-coding RNAs that may not be predicted by any of the conventional codon usage bias approaches.Transcription of DNA is highly influenced by DNA bending and flexibility. These structural properties are dependent on the base sequence [1], which in turn, is reflective of, or may influence the codon usage – also important in determining the relative expression of a given gene. Prediction of highly expressed genes and elucidation of the physical and biological properties of highly expressed genes has been addressed by a number of studies [2-4].The translational 'codon adaptation index' (CAI) is highly correlated with the expression level in fast growing bacteria [5]. It is based on the finding that highly expressed genes almost exclusively use those codons of abundant tRNAs in Escherichia coli and budding yeast [4]. Consequently for any sequenced bacterial genome, a codon bias signature can be deduced that is most likely to be efficient for translation. This bias is used to derive codon adaptation indices for all genes for a given organism, where high CAI values correspond to genes most likely to be highly expressed.However, using CAI, one is only able to predict highly expressed proteins (translated genes) since this measure is based on codon usage bias. Unfortunately, this method cannot consider tRNAs, ribosomal RNAs, and other non-coding RNAs. Moreover, for organisms with low translational bias – typically slow growing organisms – CAI is a
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