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Search Results: 1 - 10 of 67 matches for " Haiko Enok;Silvarolla "
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Caracteriza??o isoenzimática de clones e somaclones de cana-de-a?úcar
Sawazaki, Haiko Enok;Silvarolla, Maria Bernadete;Alvarez, Raphael;
Bragantia , 1989, DOI: 10.1590/S0006-87051989000100001
Abstract: extracts from leaves and roots of three sugarcane varieties and their somaclones: na56-79 (at seven and nine month old plants), iac68-12 and iac68-144 (both at nine month old plants) were obtained. the peroxidase and esterase isoenzyme contents of the extracts were determined to verify the genetic variability of tissue culture somaclones. the peroxidase specific activity was studied in relation to the time and isoenzymatic pattern. the peroxidase and esterase zymograms showed distinct differences among varieties, and the esterase presented less bands but easier to characterize than the peroxidase bands. the root zymograms exhibited great enzymatic variation due to the presence of old roots thus not allowing to characterize the varieties. no differences in peroxidase or esterase zymograms among somaclones of the same variety were noticed. the na56-79 presented lower specific activity of peroxidase and number of isoenzymes than iac68-144, suggesting that the decrease of isoenzyme bands is associated to the reduction of enzymatic activity. the specific activity of peroxidase changed with maturity and was higher at nine than at seven month old plants.
Efeitos da aplica??o de retardador (CCC) e acelerador (GA) de crescimento na morfologia e produtividade do feijoeiro (Phaseolus vulgaris L. cv. Carioca)
Castro, Paulo R. C.;Enok, Haiko;
Anais da Escola Superior de Agricultura Luiz de Queiroz , 1977, DOI: 10.1590/S0071-12761977000100019
Abstract: in this paper the autors tested the effects of (2 - chloroethyl) trimethylammonium chloride (ccc) and gibberellic acid (ga) on the cultivar carioca of bean, in three different concentrations (500, 2000, and 4000 ppm) of ccc and one concentration of ga (50 ppm). treatment with ccc 500 ppm + ga 50 ppm increased the height of bean plant and ccc 4000 ppm + ga 50 ppm reduced the height of phaseolus vulgaris. application of ccc 500 ppm + ga 50 ppm or ccc 2000 ppm + ga 50 ppm increased the leaf number of bean plant. ccc 500 ppm + ga 50 ppm promoted a increase in the internode length and ccc 4000 ppm + ga 50 ppm reduced the length of internodcs. application of ccc 500 ppm + ga 50 ppm or ccc 2000 ppm + ga 50 ppm increased the leaf length 5 days after the treatment. ccc 500 ppm + ga 50 ppm promoted higher flowering in relation to the check..
CARACTERIZA??O DA VARIABILIDADE GENéTICA EM COUVE-MANTEIGA UTILIZANDO ISOENZIMAS E RAPD
SAWAZAKI, HAIKO ENOK;NAGAI, IROSHI;SODEK, LADASLAV;
Bragantia , 1997, DOI: 10.1590/S0006-87051997000100002
Abstract: genetic variability of kale plants (brassica oleracea l. var. acephala d.c.) was studied by means of enzymatic polymorphism using polyacrilamide gel electrophoresis and a dna polymorphism assay based on rapd. fifteen clones of kale var. acephala from iac germplasm collection were studied. leaf extracts were analysed for isozymes and rapd markers using a and b kits of primers. isozyme polymorphism was observed for phosphoglucose mutase (pgm), peroxidase (prx) and esterase (est) and was higher for pgm. differences among clones were observed by isozymes and rapd, however, the dendrograms obtained from both kinds of markers were dissimilar, suggesting that the isozymes provided less information than rapd about the genome. the superior efficiency of the rapd was due to its ability to process a larger number of samples, making details about genome more evident.
CARACTERIZA O DA VARIABILIDADE GENéTICA EM COUVE-MANTEIGA UTILIZANDO ISOENZIMAS E RAPD
SAWAZAKI HAIKO ENOK,NAGAI IROSHI,SODEK LADASLAV
Bragantia , 1997,
Abstract: Estudou-se a variabilidade genética em couve (Brassica oleracea L. var. acephala D.C.) tipo manteiga por intermédio do polimorfismo enzimático em gel de poliacrilamida e do polimorfismo de DNA, denominado RAPD (Random Amplified Polymorphic DNA), com base na amplifica o de segmentos de DNA ao acaso. Avaliaram-se quinze clones de couve-manteiga do Banco Ativo de Germoplasma do Instituto Agron mico (IAC), utilizando-se extratos de folhas para análise de isoenzimas e marcador RAPD com os "primers" dos kits A e B da Operon Technologies. Entre as isoenzimas estudadas, as mais polimórficas foram as fosfoglucomutase (PGM), peroxidase (PRX) e esterase (EST), tendo o sistema PGM realizado a melhor caracteriza o. Verificou-se a ocorrência de variabilidade genética por meio de isoenzimas e RAPD, porém n o foi observada a similaridade entre os dendrogramas obtidos por ambos os tipos de marcadores, sugerindo que as isoenzimas forneceram menos informa o sobre o genoma. A maior eficácia do RAPD foi devida à possibilidade de processar maior número de análises, evidenciando mais detalhes sobre o genoma.
Caracteriza??o e identifica??o de cultivares e sele??es de pereiras através de marcadores RAPD
SAWAZAKI, HAIKO ENOK;BARBOSA, WILSON;COLOMBO, CARLOS AUGUSTO;
Revista Brasileira de Fruticultura , 2002, DOI: 10.1590/S0100-29452002000200033
Abstract: in this research, 36 pear tree accessions represented by different species, hybrids, and cultivar selections were analyzed through rapd. each primer presented 10 to 19 bands, 26 primers have provided 250 polymorphic bands from a total of 353. the opc02, opc08, opd02, opd19, opd20 and ope06 primers have identified specific bands for the oriental pears and opa01, opa11, opc08, opd04, opd09 and opd15, for the occidental pears. the obtained dendogram was confirmed by the principal coordinate analysis originating three principal groups: 1) all the pears originated by iac, like 'seleta', 'triunfo', 'primorosa', 'tenra', iac 16-41, 'centenária', besides of 'william's', 'packham's triumph', 'd'água', 'hood', 'm. sieboldt', 'kieffer', 'branca francesa' and 'schimidt'. 2) the oriental pears, such as ''okusankichi', 'shinseiki', 'atago', 'hakko', 'hosui', 'nijiseki', 'kosui' and 'ya-li', besides 'nodji', 'limeira' and all the iac selections from 193, 293 and 393 series. 3) all the rootstock from the taiwan series (p. calleryana d.) and 'manshu mamenashi' (p. betulaefolis b.). it became evident that iac cultivars have higher genetic relationship with the occidental pears (pyrus communis l.) than with the oriental pears. this fact is observed despite the fact that the iac cultivars coming from 'hood', material suspected to be an interspecific hybrid between p. communis l. and p. serotina r. these results confirm the importance the usefulness of the rapd markers in identifying cultivars, selections, and hybrids belonging to different botanical groups, showing that rapd markers are an important tool on the study of genetic diversity and genetic resource management of pears.
Caracteriza o e identifica o de cultivares e sele es de pereiras através de marcadores RAPD
SAWAZAKI HAIKO ENOK,BARBOSA WILSON,COLOMBO CARLOS AUGUSTO
Revista Brasileira de Fruticultura , 2002,
Abstract: Trinta e seis acessos de pereira representando diversas espécies, híbridos e sele es do banco de germoplasma do Instituto Agron mico (IAC) foram geneticamente caracterizados através de marcadores RAPD. Cada primer originou de 10 a 19 bandas, sendo que 26 deles forneceram 250 bandas polimórficas, de um total de 353. Os primers OPC02, OPC08, OPD02, OPD19, OPD20 e OPE06 revelaram bandas específicas para as peras orientais e OPA01, OPA11, OPC08, OPD04, OPD09 e OPD15 para as ocidentais. O dendograma obtido foi confirmado pela análise de coordenada principal, originando três principais agrupamentos: 1) Todas as pereiras lan adas pelo IAC, como 'Seleta', 'Triunfo', 'Primorosa', 'Tenra', IAC 16-41, 'Centenária', além de 'William's', 'Packham's Triumph', 'D'água', 'Hood', 'M. Sieboldt', 'Kieffer','Branca Francesa' e 'Schimidt'. 2) As pereiras asiáticas, como 'Okusankichi', 'Shinseiki', 'Atago', 'Hakko', 'Hosui', 'Nijiseiki', 'Kosui' e 'Ya-li', além de 'Nodji', 'Limeira' e todas as sele es IAC das séries 193; 293 e 393. 3) Todas as pereiras porta-enxertos da série Taiwan (P. calleryana D.), além de 'Manshu Mamenashi' (P. betulaefolia B.). Evidenciou-se que os cultivares IAC possuem maior proximidade genética com as peras ocidentais (Pyrus communis L.), mesmo sendo descendentes de 'Hood', material suspeito de ser híbrido interespecífico entre P. communis e P. serotina R.. Os resultados ratificaram a importancia dos marcadores RAPD para a identifica o de cultivares, sele es e híbridos pertencentes aos diferentes grupos botanicos, mostrando ser ferramenta de apoio adequada a programas de melhoramento genético de fruteiras.
Identification and Quantification of Corn, Soybean and Cotton Genetically Modified by Real Time PCR  [PDF]
Haiko Enok Sawazaki, Aildson Pereira Duarte, Milton Geraldo Fuzatto, Eduardo Sawazaki, Silvio Henrique Reginato Grandi, Jéssica Funari de Ponte, Larissa Nogueira
American Journal of Molecular Biology (AJMB) , 2015, DOI: 10.4236/ajmb.2015.53007
Abstract: In order to obtain a cheaper method for quantification of transgenic events in corn, soybeans and cotton, primers for real time PCR have been developed and optimized, with fluorescent BRYT Green system. The DNA was extracted from grains, with and without event, by CTAB method. The following events have been studied for corn: MON810, Bt11, MON89034, GA21, TC1507, NK603, MIR162, PRO3; Soybean: GTS-40-3-2, MON87701; MON89788; for cotton: MON1445, MON531, LLCotton25, 281-24-236; 3006-210-23, GHB614, T304-40; GHB119, MON15985, MON88913, besides the respective primers for the endogenous genes of corn, soybean and cotton. The sensitivity was 0.057%, the coefficient of linearity R2 ranged from 0.98 to 0.99 and the efficiency of PCR 0.9 to 1.1. The quantification of events ranged from 92 to 115, with a relative error (RE) from 2 to 18%, and a variance of 0.33 to 3.0. The precision acceptance criterion was observed for all analyses, as well the repeatability and reproducibility. As it was found that the measurement of accuracy and reproducibility were within the international acceptance criterion, it may infer the robustness of the methodology. Therefore, the results from replicates with two different technicians, and validation of results by comparison with those obtained by Eurofins Brazil, showed the possibility of specific and quantitative analysis of transgenic events with a cheaper method with sensitivity, repeatability and robustness.
Varia??o do teor de prolina em folhas de feij?o em fun??o da disponibilidade de água no solo
Sawazaki, Haiko Enok;Teixeira, Jo?o Paulo Feij?o;Almeida, Luiz D'Artagnan de;
Bragantia , 1981, DOI: 10.1590/S0006-87051981000100005
Abstract: the objective of this paper was to verify the accumulation of free proline in leaves of beans (phaseolus vulgaris l.) when subjected to water stress. leaves samples were taken at 11 and 15 days after starting the water stress and 4 days after irrigation to estimate the proline accumulation. the results obtained showed differences among bean cultivars in the proline content and the capacity for accumulation of this aminoacid under 15 days of water stress. 'jalo', 'roseli', and 'rox?o lustroso' were cultivars with the highest proline accumulation (> 7 mmol/g dw) whereas 'moruna' and 'curitibano bairro das palmeiras' were cultivars with the lowest proline content (< 3 mmol/g dw), after 15 days of water stress. the addition of water after 11 days of water stress showed proline content similar to the irrigated plants. the materials studied were classified as a function of their capacity for proline accumulation following 15 days of water stress. this classification represents differences in drought resistance if a higher proline contents is considered a measurement of the plant adaptation to the unfavorable condition.
Avalia??o da atividade da lipoxigenase em linhagens de soja
Sawazaki, Haiko Enok;Teixeira, Jo?o Paulo Feij?o;Miranda, Manoel Albino Coelho de;
Bragantia , 1987, DOI: 10.1590/S0006-87051987000200017
Abstract: seed of soybeans, glycine max l. (merrill), cultivar iac-8 and lines a-5, low linolenic acid content, pi 408251, lacking l1 mutant, pi 86023, lacking l2 mutant, tohoko no 74, lacking l3 mutant, and f2 population from the crosses between iac-8 and mutant lines (lacking l1, l2 and l3) were analysed for linolenic acid content, lipoxygenase activity and identification of mutant lines lacking l1, l2 or l3 isoenzymes. the purpose was to evaluate methods to identify mutants lines of soybean for lipoxygenase and to verify a possible correlation between enzimatic activity and linolenic acid content. the enzimatic activity was estimated quantitatively by a colorimetric method and lipoxygenase isoenzymes detection by isoelectric focusing. the oil seed content was determined by gravimetry and the fatty acids content by gas liquid chromatography of their methyl esters. the lipoxygenase activity data was good to indicate mutant lines for the final identification using isoelectric focusing. it permits the use of this method for choosing lines in breeding program to obtain a good soybean oil stability. it was not observed correlation, in the material studied, between enzymatic activity and linolenic acid content.
Transporte de compostos nitrogenados em soja cultivada com diferentes fontes de nitrogênio
Sawazaki, Haiko Enok;Sodek, Ladaslav;Teixeira, Jo?o Paulo Feij?o;
Bragantia , 1987, DOI: 10.1590/S0006-87051987000200011
Abstract: soybean plants, inoculated with rhizobium japonicum, were grown in vermiculite with a nitrogen free nutrient solution in a greenhouse, in the experimental station of campinas, instituto agron?mico, state of s?o paulo, brazil. the effects of no3- and nh4+ were studied in terms of ureide and amino acid levels in the xylem sap. nodulated plants were treated with nutrient solution either containing no3- (15 mm), or nh4+ (10 mm), or without nitrogen (control) for 7 days, near the flowering period. higher proportion of alantoic acid was found relative to allantoin, and this proportion increased in plants grown on mineral n. the nh4+ ion had an intermediary effect compared to the other n-assimilatory processes, with respect to the quantity of n transported to the aerial parts, suggesting that the mecanism of transport depended on the interaction between the soil mineral nitrogen and the energy available. asparagin was the amino acid in the greatest quantity in the xylem, independent of the treatment. ammonium did not alter the levels of n-nh4+ in the sap, but increased slightly the level of no3-. nitrate caused slight increase in aspartic acid and large in no3- content in the sap. these results suggested that the differences in the amino acid synthesis proceeded of a specific pathway of nitrogen uptake.
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