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Search Results: 1 - 10 of 320122 matches for " Héctor de Paz-Carmona "
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High Blood Pressure Effects on the Brain Barriers and Choroid Plexus Secretion  [PDF]
Ibrahim González-Marrero, Leandro Casta?eyra-Ruiz, Juan M. González-Toledo, Agustín Casta?eyra-Ruiz, Héctor de Paz-Carmona, Lidia Ruiz-Mayor, Agustín Casta?eyra-Perdomo, Emilia M. Carmona-Calero
Neuroscience & Medicine (NM) , 2012, DOI: 10.4236/nm.2012.31009
Abstract: High blood pressure produces ventricular dilation, variations in circumventricular organs and changes in the cerebrospinal fluid compositions. On the other hand, chronic hypertension in spontaneously hypertensive rats can cause changes in the integrity of the brain barriers: blood-cerebrospinal fluid barrier and blood brain barrier. The permeability of the brain barriers can be studied by using transthyretin and S-100β. In the present work we study the integrity of the brain barrier and the choroid plexus function variations in arterial hypertension. Control rats and spontaneously hypertensive rats were used and the choroid plexus were processed by immunohistochemistry with anti-transthyretin and anti-vasopressin. Western blot was also performed in cerebrospinal fluid, serum and choroid plexus using anti-S-100β, anti-transthyretin. The accumulation of transthyretin immunoreactive was bigger in spontaneously hypertensive rats with respect to the control. Vasopressin was also higher in spontaneously hypertensive rats with respect to the control. Western blot showed that transthyretin tetramer was higher in the spontaneously hypertensive rats than in the control rats. The expression of transthyretin monomer was lower in hypertensive rats than the control in the cerebrospinal fluid, the transthyretin monomer reaction in the blood was stronger in hypertensive than in control rats. Western blot for the S-100 β showed an increase in blood and cerebrospinal fluid of hypertensive rats. The high blood pressure produces a disruption of the blood brain barrier and blood to cerebrospinal fluid barrier that allows extravasations from the cerebrospinal fluid to the blood and from the blood to the cerebrospinal fluid.
Hypertension effects on p73 expression in the rat circumventricular organs and cerebrospinal fluid  [PDF]
Emilia M. Carmona-Calero, Ibrahim González-Marrero, Manuela Casta?eyra-Martin, Juan M. González-Toledo, Leandro Casta?eyra-Ruiz, Héctor de Paz-Carmona, Agustín Casta?eyra-Ruiz, Lidia Ruiz-Mayor, Agustín Casta?eyra-Perdomo
World Journal of Neuroscience (WJNS) , 2012, DOI: 10.4236/wjns.2012.22010
Abstract: It has been reported that spontaneously hypertensive rats (SHR) show ventricular dilation, changes in CSF proteins and variations in the circumventricular organs (CVO) such as: the subcommissural organ (SCO), the subfornical organ (SFO) and the area postrema (AP) which are located in the walls of the third and fourth ventricles. On the other hand, p73 proteins are present in cells of the central nervous system (CNS) such as circumventricular structures and the neuroepithelium which are altered in ventricular dilation. The purpose of the present work is to study the TAp73 isoform expression in the circumventricular organs (CVO) and their variations in ventricular dilatation and arterial hypertension. Brains and cerebrospinal fluid (CSF) from control Wistar-Kyoto rats (WKY) and SHR were used. The paraffin sections containing the CVO were immunohistochemically proc-essed with anti-TAp73 and by western blot, p73 bands in the CSF and circumventricular organ extract were also identified. The western blot study showed bands marked with p73 in the CSF and CVO, the p73 band expression was bigger in the SHR than in the WKY rats. We also found stronger markings in the SFO, SCO and AP of the hypertensive rats than in the WKY rats. It could be concluded that hypertension in the SHR produces altera-tions in the relationship between the p73 protein, circumventricular structures and CSF.
Luteinizing Hormone-Releasing Hormone Distribution in the Anterior Hypothalamus of the Female Rats
Leandro Casta?eyra-Ruiz,Ibrahim González-Marrero,Agustín Casta?eyra-Ruiz,Juan M. González-Toledo,María Casta?eyra-Ruiz,Héctor de Paz-Carmona,Agustín Casta?eyra-Perdomo,Emilia M. Carmona-Calero
ISRN Anatomy , 2013, DOI: 10.5402/2013/870721
Abstract: Luteinizing hormone-releasing hormone (LHRH) neurons and fibers are located in the anteroventral hypothalamus, specifically in the preoptic medial area and the organum vasculosum of the lamina terminalis. Most luteinizing hormone-releasing hormone neurons project to the median eminence where they are secreted in the pituitary portal system in order to control the release of gonadotropin. The aim of this study is to provide, using immunohistochemistry and female brain rats, a new description of the luteinizing hormone-releasing hormone fibers and neuron localization in the anterior hypothalamus. The greatest amount of the LHRH immunoreactive material was found in the organum vasculosum of the lamina terminalis that is located around the anterior region of the third ventricle. The intensity of the reaction of LHRH immunoreactive material decreases from cephalic to caudal localization; therefore, the greatest immunoreaction is in the organum vasculosum of the lamina terminalis, followed by the dorsomedial preoptic area, the ventromedial preoptic area, and finally the ventrolateral medial preoptic area, and in fibers surrounding the suprachiasmatic nucleus and subependymal layer on the floor of the third ventricle where the least amount immunoreactive material is found. 1. Introduction The luteinizing hormone-releasing hormone (LHRH) is a gonadotropin releasing hormone (GnRH), which acts on the pituitary hormones as a follicle stimulating hormone (FSH) and luteinizing hormone (LH), which act on the gonads, [1]. The GnRH neurons are originated in the nasal epithelium and migrate accompanying the fibers of the vomeronasal and terminal nerves [2, 3] up to the anterobasal part of the brain, where they enter the brain together with nerve terminals and then move caudally to the preoptic hypothalamus, where GnRH neurons are definitively located [2, 4]. These GnRH neurons and fibers are mainly located in the anteroventral third ventricle region, specifically in the preoptic area (PA) and the organum vasculosum of the lamina terminalis (OVLT) [5]. The anterior hypothalamus is the major region of the diencephalon implicated in the development of the olfactory system and the sexual differentiation of the brain. Most of the GnRH neurons axons project to the external zone of the median eminence where is GnRH secreted into the pituitary portal vasculature to control the release of gonadotropin [6–8]. The preoptic area (PA) is part of the anterior hypothalamus and is confined to the anteroventral region of the third ventricle (AV3V); the PA is divided into, the medial
Angiotensin II, Vasopressin, and Collagen-IV Expression in the Subfornical Organ in a Case of Syndrome of Inappropriate ADH
Emilia M. Carmona-Calero,Juan M. González-Toledo,Leandro Casta?eyra-Ruiz,Ibrahim González-Marrero,María Casta?eyra-Ruiz,Héctor de Paz-Carmona,Agustín Casta?eyra-Ruiz,Nélida Rancel-Torres,Agustín Casta?eyra-Perdomo
Advances in Endocrinology , 2014, DOI: 10.1155/2014/179795
Abstract: The syndrome of inappropriate antidiuretic hormone (SIADH) is a disease characterized by hyponatremia and hyperosmolarity of urine where vasopressin and angiotensin II are implicated in the alteration of salt water balance and cardiovascular and blood pressure regulation. The aim of this study is to analyse the expression of substances related with cardiovascular and salt water regulation in the subfornical organ in a case of SIADH. Two brains, one taken from a 66-year-old man with SIADH and the other from a 63-year-old man without SIADH, were used. Immunohistochemical study was performed using anti-angiotensin II, anti-vasopressin, and anti-collagen-VI as primary antibodies. Angiotensin and vasopressin immunoreaction were found in neurons, in perivascular spaces, and in the ependymal layer in the subfornical organ in both cases. However, in the SIADH case, the angiotensin II and collagen-IV expression in the SFO were different suggesting this organ’s possible participation in the physiopathology of SIADH. 1. Introduction Diabetes insipidus (DI), syndrome of inappropriate antidiuretic hormone (SIADH), and syndrome cerebral salt-wasting (CSW) are three pathologies with hyponatremia and hyperosmolarity and differentiation between them is important to prescribe the most appropriate treatments [1, 2]. SIADH is a disease which is characterized by the hyponatremia and hyperosmolarity of urine [3–5]. There are well known causes for this syndrome, such as neoplasmatic processes, disorders of the central nervous system, lung diseases, and the side effects of drugs. A study [6] of a large group of patients has revealed that SIADH occurs in 3% of patients with head and neck cancer, in 0.7% of patients with non-small-cell lung cancer, and in 15% of cases of small-cell lung cancer [6]. The standard therapy for SIADH is to treat the underlying malignant disease. If this is not possible or if the disease has become refractory, other treatment methods are available such as water restriction, demeclocycline therapy, or, in severe cases, infusion of hypertonic saline together with furosemide during treatment [6]. Total body water and tonicity are strictly regulated by the renal action of the antidiuretic hormone (ADH), renin-angiotensin-aldosterone system, and norepinephrine and by the thirst mechanism. Abnormalities in water balance are manifested in SIADH as sodium disturbances-hyponatremia and hypernatremia [6]. On the other hand, the presence of VAS, AGII, and TH and their implication in cardiovascular, salt water balance and blood pressure regulation have long been
High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats
Ibrahim González-Marrero,Leandro Casta?eyra-Ruiz,Juan M. González-Toledo,Agustín Casta?eyra-Ruiz,Hector de Paz-Carmona,Rafael Castro,Juan R. Hernandez-Fernaud,Agustín Casta?eyra-Perdomo,Emilia M. Carmona-Calero
International Journal of Hypertension , 2013, DOI: 10.1155/2013/164653
Abstract: The aim of the present work is to analyze the cerebrospinal fluid proteomic profile, trying to find possible biomarkers of the effects of hypertension of the blood to CSF barrier disruption in the brain and their participation in the cholesterol and β-amyloid metabolism and inflammatory processes. Cerebrospinal fluid (CSF) is a system linked to the brain and its composition can be altered not only by encephalic disorder, but also by systemic diseases such as arterial hypertension, which produces alterations in the choroid plexus and cerebrospinal fluid protein composition. 2D gel electrophoresis in cerebrospinal fluid extracted from the cistern magna before sacrifice of hypertensive and control rats was performed. The results showed different proteomic profiles between SHR and WKY, that α-1-antitrypsin, apolipoprotein A1, albumin, immunoglobulin G, vitamin D binding protein, haptoglobin and α-1-macroglobulin were found to be up-regulated in SHR, and apolipoprotein E, transthyretin, α-2-HS-glycoprotein, transferrin, α-1β-glycoprotein, kininogen and carbonic anhidrase II were down-regulated in SHR. The conclusion made here is that hypertension in SHR produces important variations in cerebrospinal fluid proteins that could be due to a choroid plexus dysfunction and this fact supports the close connection between hypertension and blood to cerebrospinal fluid barrier disruption. 1. Introduction Cerebrospinal fluid is a functional system closely connected to the brain, and changes or variations in the CSF may mean an alteration in the brain expressed by encephalic disorders. However, the composition of CSF may also be altered by systemic diseases, such as arterial hypertension, and cerebral ventricular dilatation, changes in CSF protein, and variations of the choroid plexus and other circumventricular organs (CVO) have been described in spontaneously hypertensive rats (SHR) [1–5]. Therefore, SHR develop hydrocephalus and experimental studies explain that hydrocephalus induces alterations in CSF since there are disturbances, in the hydrocephalic brain, of oxidative metabolism and neurotransmission and perhaps damage to periventricular cells, particularly when intracranial pressure is elevated [6]. The sharp increase in systemic blood pressure only causes an acute increase in CSF pressure in normotensive animals and not in hypertensive patients [6]. The CSF pressure of SHR showed greater protection to the acute effects of phenylephrine than in control Wistar-Kyoto (WKY) rats, but a permeability increase of the blood to cerebrospinal fluid barrier to sucrose in
Leptospirosis humana: un problema de salud
Beatriz Rodríguez Alonso,Héctor José Gómez de Haz,Raúl Cruz de la Paz
Revista Cubana de Salud Pública , 2000,
Abstract: Se expone un estudio que aborda los antecedentes históricos de la leptospirosis humana en Cuba y en el mundo. Se presenta la situación actual de la enfermedad en algunos países de Europa, Asia, áfrica, Oceanía y América, que nos hace pensar que la leptospirosis constituye un problema de salud mundial. A study dealing with the history of human leptospirosis in Cuba and in the world is put into the consideration of the readers. The present situation of this disease in some countries of Europe, Asia, Africa, Oceania and the Americas makes us think that leptospirosis is a world health problem.
Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
Mendoza,Héctor; de la Jara,Adelina; Freijanes,Karen; Carmona,Laura; Ramos,Ana Alexandra; de Sousa Duarte,Vanessa; Serafim Varela,Jo?o Carlos;
Electronic Journal of Biotechnology , 2008,
Abstract: in the present work the characterization of different strains of dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the canary island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. results showed a high morphological and physiological intraspecific variability among the studied strains. results suggest that the application of the lipidic dye nile red allowed the development of a cytometric method faster than the traditional techniques to select carotenoid hyperproducing strains of microalgae.
Molecular taxonomy of Dunaliella (Chlorophyceae), with a special focus on D. salina: ITS2 sequences revisited with an extensive geographical sampling
Patrícia Assun??o, Ruth Jaén-Molina, Juli Caujapé-Castells, Adelina de la Jara, Laura Carmona, Karen Freijanes, Héctor Mendoza
Aquatic Biosystems , 2012, DOI: 10.1186/2046-9063-8-2
Abstract: The Internal Transcribed Spacer 2 (ITS2) of the nuclear rDNA cistron is one of the most frequently used regions for phylogenetic analysis in algae [1-3]. Although its application in deep taxonomic levels was initially limited to comparisons of genera within the same family owing to uncertainties in alignment at higher taxonomic levels, the analysis of its secondary structure has provided key solutions to this problem [4]. Thus, the use of an ITS2 secondary structure improves sequence alignments, resulting in a higher robustness and accuracy of phylogenetic reconstructions [5] and providing help to distinguish species [6]. Furthermore, an automatic approach to analysis is possible [7], as a pipeline consisting of the ITS2 Database (annotation/structure prediction), 4SALE (alignment), ProfDistS (inferring phylogenies) and the CBCAnalyzer (distinguishing species) have recently become available (http://its2.bioapps.biozentrum.uni-wuerzburg.de/?about webcite).In Dunaliella (Chlorophyceae), the use of ITS2 secondary structure for phylogenetic analysis has a long tradition [8-13]. The genus Dunaliella comprises twenty-eight recognized species separated in two subgenera, Pascheria (which contains the freshwater species), and Dunaliella (grouping the marine species); the latter is further subdivided into four sections: Tertiolecta, Dunaliella, Viridis and Peirceinae [11]. The species ascribed to these four sections occur in a wide range of marine habitats such as oceans, brine lakes, salt marshes, salt lagoons and salt water dishes near the sea [14], being Dunaliella salina Teodoresco (section Dunaliella) the most studied one. Dunaliella salina, is the most halotolerant eukaryotic photosynthetic organism known to date [14,15] since it shows a remarkable degree of adaptation to a variety of salt concentrations and it accumulates large amounts of carotenes under extremely stressful conditions such as high salinity, low nitrogen levels, and high solar radiation [14]. Nowadays,
Algunos aspectos clinicoepidemiológicos en fallecidos por leptospirosis humana en Ciudad de La Habana
Rodríguez Alonso,Beatriz; Gómez de Haz,Héctor José; Pérez Maza,Benito; Cruz de la Paz,Raúl;
Revista Cubana de Medicina General Integral , 2001,
Abstract: a descriptive restrospective study was conducted in 9 clinical and surgical hospitals of havana city. the medical histories of the dead due to human leptospirosis from 1992 to 1997 were reviewed. the studied variables were: age, sex, source of infection, type of contact, season of the year, the most frequent symptoms and signs and associated diseases. the predominance of male dead individuals over 50 was among the main results. the main source of infection was pig breeding. all those who died had fever and myalgias in the lower limbs
Diagnóstico y Tratamiento de la Leptospirosis Humana
Rodríguez Alonso,Beatriz; Gómez de Haz,Héctor José; Pérez Maza,Benito; Cruz de la Paz,Raúl;
Revista Cubana de Medicina General Integral , 2001,
Abstract: a review is made on the topic of human leptospirosis, since it is a health problem in our country and in many other parts of the world. we take into consideration the analysis of the essential milestones for the diagnosis of any entity: epidemiology, clinical picture and laboratory. the importance of the anatomopathological diagnosis in the dead due to human leptospirosis is also taken into account. the pathognomonic aspects in relation to this disease are given here. likewise, the current schemes of the elective treatment for cuba are dealt with
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