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Phylogenic Study of Twelve Species of Phyllanthus Originated from India through Molecular Markers for Conservation  [PDF]
Gyana Ranjan Rout, Subhashree Aparajita
American Journal of Plant Sciences (AJPS) , 2010, DOI: 10.4236/ajps.2010.11005
Abstract: The objective of the study was to characterize the germplasm for identification and phylogeny study for conservation. Identification and characterization of germplasm is an important link between the conservation and utilization of plant genetic resources. The present investigation was undertaken to draw the phylogenetic relationship between twelve species from India belonging to genus Phyllanthus with the help of molecular markers. In total, 259 marker loci were assessed, out of which 249 were polymorphic revealing 96.13% polymorphism. Nei’s similarity index varies from 0.23 to 0.76 for RAPD and 0.26 to 0.81 for ISSR marker systems. Cluster analysis by unweighted pair group method (UPGMA) of Dice coefficient of similarity generated dendogram with more or less similar topology for both the analysis that gave a better reflection of diversity and affinities between the species. The phylogenetic tree obtained from both RAPD and ISSR marker has divided the 12 species in two groups: group I consisting of only one species Phyllanthus angustifolius and the group II with the rest 11 species. This molecular result is comparable to notable morphological characteristics. The present study revealed the distant variation within the species of Phyllanthus. This investigation will help for identification and conservation of Phyllanthus species.
Agromorphological and Molecular Characterization of Sesamum indicum L.—An Oil Seed Crop  [PDF]
Tapaswini Hota, Chinmaya Pradhan, Gyana Ranjan Rout
American Journal of Plant Sciences (AJPS) , 2016, DOI: 10.4236/ajps.2016.717210
Abstract: Sesamum indicum L. (family: Pedaliaceae) is an economically important oil seed crop grown in tropical and sub-tropical countries. It is widely used in food, nutraceutical, pharmaceutical industries. Sesamum is widely distributed in all the climatic stages and great diversity. The exploration of genetic diversity is a pre-requisite for genome organization in the landraces and the related domesticated ones. Agromorphological and molecular markers were used to assess the identification of 33 Sesamum genotypes and determination of the genetic relationships among these genotypes. Out of 30 Inter-Simple Sequence Repeat (ISSR) primers tested, 18 primers produced 114 detectable fragments, of which 97 (85.08%) were polymorphic across the varieties. Molecular profiling could be solely used for their identification of genotypes. Genetic relationships among these genotypes were evaluated by generating a similarity matrix based on the Jacard’s coefficient and the Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendogram. The results showed a clear cut separation of the 33 genotypes and were in broad agreement with the morphology. Both molecular and morphological markers will be useful for preservation of the germplasm as well as breeding program.
Characterization of new variety of Chrysanthemum by using ISSR markers
Palai, SK;Rout, Gyana Ranjan;
Horticultura Brasileira , 2011, DOI: 10.1590/S0102-05362011000400029
Abstract: chrysanthemum is the important cut flower after rose among the ornamental plants traded in the global flower market. it is propagated vegetatively and also has a strong sporophytic self-incompatibility system as shown by all members of asteraceae family. morphologically, the petal numbers and flower colours present maximum variation when compared to existing varieties. twenty inter simple sequence repeat primers were used to detect the new variety of chrysanthemum developed through spontaneous sporting. the results indicate that the rate of polymorphism showed significant differences as compared to other existing varieties. the average number of amplification products per primer was eight. the size of issr amplified fragments varied from 0.25 - 2.4 kbp. therefore, issr marker is a useful technique for the rapid and easy assessment of genetic variation among the variants. morphological traits of new variants showed variation as compared to other parents. the 1st flower bud appearance and the height of 1st bud of the variant were less as compared to original mother variety. the new variants can be propagated in large scale commercially through in vitro technique.
Studies on Inter and intra-population variability of Pongamia pinnata: a bioenergy legume tree
Gyana Ranjan Rout,Debee Prasad Sahoo,Subhashree Aparajita
Crop Breeding and Applied Biotechnology , 2009,
Abstract: Pongamia pinnata is an oil producing tree species with multiple uses and considerable potential as a bioenergycrop. The present investigation has been carried out to assess the extent of genetic structure in a representative set of 111individuals of P. pinnata encompassing seven populations as a prelude for utilization of promising and genetically divergentmaterial in the breeding program. Molecular polymorphism was 67.18% with 10 Inter Simple Sequence Repeats (ISSR)between the individuals indicating modest levels of genetic variation in the P. pinnata germplasm collected. The withinpopulation variation based on ISSR polymorphism was 32.34% and polymorphism at the species level was 94.34%. Geneticdifferentiation between populations (GST= 0.61) was positively correlated with geographical distance. The data obtainedindicated an immediate need for widening the genetic base of P. pinnata germplasm for proper characterization and extensiveplantations of elite varieties to meet the biodiesel demands.
Genotypic Variability and Correlation Studies in Pod and Seed Characteristics of Pongamia pinnata (L.) Pierre in Orissa, India
Deebe Prasad Sahoo,Gyana Ranjan Rout,Swarnalata Das,Subhashree Aparajita,A. K. Mahapatra
International Journal of Forestry Research , 2011, DOI: 10.1155/2011/728985
Abstract: Pongamia pinnata (L.) Pierre is a fast growing leguminous tree with high potential for oil seed production. Fifty-three candidate plus trees (CPTs) of Pongamia pinnata were selected from different locations in Orissa, India, on the basis of their seed and pod characteristics to identify suitable seed source with high oil content for production of quality planting seedling for use in afforestation programs. All the CPTs showed significant variation among themselves in respect to their pod and seed characters. Phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) estimates were high for pod thickness, seed thickness, 100-pod weight, and 100-seed weight. High heritability values accompanied by high genetic advance for 100-seed weight (96.1%, 59.6) and 100-pod weight (90.9%, 37.3) indicated additive gene action. High estimates of genotypic correlations than the corresponding phenotypic correlations indicated the presence of strong inherent association between pod length and pod breadth; 100-pod weight, and pod thickness; 100-pod weight and seed length; 100-seed weight and 100-pod weight. Seed length, seed breadth, seed thickness, 100-pod weight and 100-seed weight had significant positive correlation with each other, and these characters should be considered as effective parameters to select CPTs for different agroforestry programs. 1. Introduction Self-reliance in energy is vital for overall economic development of India and other developing countries. The recent oil crises and depleting fossil fuel reserves have rekindled interest in promotion of tree-borne oil seed species. Amongst the many plant species, Pongamia pinnata—a fast growing leguminous tree that has a high potential for high oil seed production and ability to grow on marginal lands—supports its cultivation as a potential biofuel crop for biodiesel industry [1]. Pongamia pinnata (L.) Pierre is an arboreal legume belonging to subfamily Papilionoideae. The medium size tree is indigenous to the Indian subcontinent and South-East Asia and has been successfully introduced to humid tropical regions including parts of Australia, New Zealand, China, and United States. The mature tree can withstand water logging and slight frost and is highly tolerant to salinity, and it can be grown along seashores with its roots surviving in saltwater. In its natural habitat, the species tolerates a wide range of temperature that is up to 50°C. P. pinnata had also a positive bioameliorative effect with contributions to soil nitrogen, phosphorous, potassium, and organic carbon. It is a
Enhancement of antidandruff activity of shampoo by biosynthesized silver nanoparticles from Solanum trilobatum plant leaf
Gaurav Pant,Nitesh Nayak,R. Gyana Prasuna
Applied Nanoscience , 2013, DOI: 10.1007/s13204-012-0164-y
Abstract: The present investigation describes simple and effective method for synthesis of silver nanoparticles via green route. Solanum trilobatum Linn extract were prepared by both conventional and homogenization method. We optimized the production of silver nanoparticles under sunlight, microwave and room temperature. The best results were obtained with sunlight irradiation, exhibiting 15–20 nm silver nanoparticles having cubic and hexagonal shape. Biosynthesized nanoparticles were highly toxic to various bacterial strains tested. In this study we report antibacterial activity against various Gram negative (Klebsiella pneumoniae, Vibrio cholerae and Salmonella typhi) and Gram positive (Staphylococcus aureus, Bacillus cereus and Micrococcus luteus) bacterial strains. Screening was also performed for any antifungal properties of the nanoparticles against human pathogenic fungal strains (Candida albicans and Candida parapsilosis). We also demonstrated that these nanoparticles when mixed with shampoo enhance the anti-dandruff effect against dandruff causing fungal pathogens (Pityrosporum ovale and Pityrosporum folliculitis). The present study showed a simple, rapid and economical route to synthesize silver nanoparticles and their applications hence has a great potential in biomedical field.
Synthesis, Characterization, Biological Activity and DNA Binding Studies of Metal Complexes with 4-Aminoantipyrine Schiff Base Ligand
B. Anupama,M. Padmaja,C. Gyana Kumari
Journal of Chemistry , 2012, DOI: 10.1155/2012/291850
Assessment of Spatial Variation of Groundwater Quality and Its Relationship With Land Use in Perth Metropolitan  [PDF]
Priyantha Ranjan SARUKKALIGE
Journal of Water Resource and Protection (JWARP) , 2011, DOI: 10.4236/jwarp.2011.35039
Abstract: To determine the effects of land use on groundwater quality in Western Australia, a quantitative analysis is carried out using groundwater quality data supplied by the Department of Water from over 500 groundwater wells across the Perth metropolitan area. We analyzed four main groundwater quality indicators; nutrients, physical parameters, inorganic non metals and trace metals. We found that groundwater beneath agricultural land was found to be particularly susceptible to nutrient loading due to the application of fertilizers. Nutrient levels were found to be rising over time due to increasing agriculture and urban development. Industrial areas were also found to have numerous contamination plumes that continue to migrate with the groundwater flow. According to Australian and New Zealand Environment and Conservation Council (ANZECC) guidelines and the Australian Drinking Water Guidelines (ADWG), several areas including rural areas like Carabooda lake, Gnangara and Jandakot Mounds, Cockburn Sound, Forrestdale, Joondalup, and Ellenbrook and high density urban areas like Balcatta and Neerabup, industrial areas like North Fremantle, Welshpool and Kwinana are indentified as the vulnerable areas for groundwater quality.
Variations of Enclosing Problem Using Axis Parallel Square(s): A General Approach  [PDF]
Priya Ranjan Sinha Mahapatra
American Journal of Computational Mathematics (AJCM) , 2014, DOI: 10.4236/ajcm.2014.43016

Let P be a set of n points in two dimensional plane. For each point \"\", we locate an axis- parallel unit square having one particular side passing through p and enclosing the maximum number of points from P. Considering all points \"\", such n squares can be reported in O(nlogn) time. We show that this result can be used to (i) locate m>(2) axis-parallel unit squares which are pairwise disjoint and they together enclose the maximum number of points from P (if exists) and (ii) find the smallest axis-parallel square enclosing at least k points of P , \"\".

Search for Monic Irreducible Polynomials with Decimal Equivalents of Polynomials over Galois Field GF(pq)  [PDF]
Sankhanil Dey, Ranjan Ghosh
Open Journal of Discrete Mathematics (OJDM) , 2018, DOI: 10.4236/ojdm.2018.81003
Abstract: Substitution boxes or S-boxes play a significant role in encryption and de-cryption of bit level plaintext and cipher-text respectively. Irreducible Poly-nomials (IPs) have been used to construct 4-bit or 8-bit substitution boxes in many cryptographic block ciphers. In Advance Encryption Standard, the ele-ments of 8-bit S-box have been obtained from the Multiplicative Inverse (MI) of elemental polynomials (EPs) of the 1st IP over Galois field GF(28) by adding an additive element. In this paper, a mathematical method and the algorithm of the said method with the discussion of the execution time of the algorithm, to obtain monic IPs over Galois field GF(pq) have been illustrated with example. The method is very similar to polynomial multiplication of two polynomials over Galois field GF(pq) but has a difference in execution. The decimal equivalents of polynomials have been used to identify Basic Polynomials (BPs), EPs, IPs and Reducible polynomials (RPs). The monic RPs have been determined by this method and have been cancelled out to produce monic IPs. The non-monic IPs have been obtained with multiplication of α where?α∈ GF(pq)?and assume values from 2 to (p 1) to monic IPs.
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