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Search Results: 1 - 10 of 120195 matches for " Gwo-Jaw Wang "
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Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2
Jyun-Yi Wu, Yan-Hsiung Wang, Gwo-Jaw Wang, Mei-Ling Ho, Chau-Zen Wang, Ming-Long Yeh, Chia-Hsin Chen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044027
Abstract: Low-power laser irradiation (LPLI) has been found to induce various biological effects and cellular processes. Also, LPLI has been shown to promote fracture repair. Until now, it has been unclear how LPLI promotes bone formation and fracture healing. The aim of this study was to investigate the potential mechanism of LPLI-mediated enhancement of bone formation using mouse bone marrow mesenchymal stem cells (D1 cells). D1 cells were irradiated daily with a gallium-aluminum-arsenide (GaAlAs) laser at dose of 0, 1, 2, or 4 J/cm2. The lactate dehydrogenase (LDH) assay showed no cytotoxic effects of LPLI on D1 cells, and instead, LPLI at 4 J/cm2 significantly promoted D1 cell proliferation. LPLI also enhanced osteogenic differentiation in a dose-dependent manner and moderately increased expression of osteogenic markers. The neutralization experiments indicated that LPLI regulated insulin-like growth factor 1 (IGF1) and bone morphogenetic protein 2 (BMP2) signaling to promote cell proliferation and/or osteogenic differentiation. In conclusion, our study suggests that LPLI may induce IGF1 expression to promote both the proliferation and osteogenic differentiation of D1 cells, whereas it may induce BMP2 expression primarily to enhance osteogenic differentiation.
A Novel Single Pulsed Electromagnetic Field Stimulates Osteogenesis of Bone Marrow Mesenchymal Stem Cells and Bone Repair
Yin-Chih Fu, Chih-Chun Lin, Je-Ken Chang, Chung-Hwan Chen, I-Chun Tai, Gwo-Jaw Wang, Mei-Ling Ho
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091581
Abstract: Pulsed electromagnetic field (PEMF) has been successfully applied to accelerate fracture repair since 1979. Recent studies suggest that PEMF might be used as a nonoperative treatment for the early stages of osteonecrosis. However, PEMF treatment requires a minimum of ten hours per day for the duration of the treatment. In this study, we modified the protocol of the single-pulsed electromagnetic field (SPEMF) that only requires a 3-minute daily treatment. In the in vitro study, cell proliferation and osteogenic differentiation was evaluated in the hBMSCs. In the in vivo study, new bone formation and revascularization were evaluated in the necrotic bone graft. Results from the in vitro study showed no significant cytotoxic effects on the hBMSCs after 5 days of SPEMF treatment (1 Tesla, 30 pulses per day). hBMSC proliferation was enhanced in the SPEMF-treated groups after 2 and 4 days of treatment. The osteogenic differentiation of hBMSCs was significantly increased in the SPEMF-treated groups after 3–7 days of treatment. Mineralization also increased after 10, 15, 20, and 25 days of treatment in SPEMF-treated groups compared to the control group. The 7-day short-course treatment achieved similar effects on proliferation and osteogenesis as the 25-day treatment. Results from the in vivo study also demonstrated that both the 7-day and 25-day treatments of SPEMF increased callus formation around the necrotic bone and also increased new vessel formation and osteocyte numbers in the grafted necrotic bone at the 2nd and 4th weeks after surgery. In conclusion, the newly developed SPEMF accelerates osteogenic differentiation of cultured hBMSCs and enhances bone repair, neo-vascularization, and cell growth in necrotic bone in mice. The potential clinical advantage of the SPEMF is the short daily application and the shorter treatment course. We suggest that SPEMF may be used to treat fractures and the early stages of osteonecrosis.
Blur Image Edge to Enhance Zernike Moments for Object Recognition  [PDF]
Chihying Gwo, Anwen Deng
Journal of Computer and Communications (JCC) , 2016, DOI: 10.4236/jcc.2016.415007
Abstract:
Zernike moments (ZMs) are a set of orthogonal moments which have been successfully used in the fields of image processing and pattern recognition. A combination of edge blurring with ZMs computation was introduced. In this study, several kinds of artificial binary stripe images were used to investigate the effects of edge blurring on the absolute mean error of reconstructed image from high-order ZMs. After the blurring process, the reconstruction errors were increased dramatically at edge pixels, but decreased on non-edge pixels. The experimental results demonstrated that 2-pixel blurring approach provided better performance for reducing reconstruction error. Finally, a template matching between two real images was simulated to illustrate the effectiveness of the proposed method.
Chemical Sensing Sensitivity of Long-Period Grating Sensor Enhanced by Colloidal Gold Nanoparticles
Jaw-Luen Tang,Jien-Neng Wang
Sensors , 2008, DOI: 10.3390/s8010171
Abstract: A simple and effective method is proposed to improve spectral sensitivity anddetection limit of long period gratings for refractive index or chemical sensing, where thegrating surface is modified by a monolayer of colloidal gold nanoparticles. Thetransmission spectra and optical properties of gold nanospheres vary with the differentrefractive index of the environment near the surface of gold nanospheres. The sensorresponse of gold colloids increases linearly with solvents of increasing refractive index.The results for the measurement of sucrose and sodium chloride solutions are reported,which show that this type of sensor can provide a limiting resolution of ~10-3 to ~10-4 forrefractive indices in the range of 1.34 to 1.39 and a noticeable increase in detection limit ofrefractive index to external medium.
Error Analysis and Measurement Uncertainty for a Fiber Grating Strain-Temperature Sensor
Jaw-Luen Tang,Jian-Neng Wang
Sensors , 2010, DOI: 10.3390/s100706582
Abstract: A fiber grating sensor capable of distinguishing between temperature and strain, using a reference and a dual-wavelength fiber Bragg grating, is presented. Error analysis and measurement uncertainty for this sensor are studied theoretically and experimentally. The measured root mean squared errors for temperature T and strain ε were estimated to be 0.13 °C and 6 με, respectively. The maximum errors for temperature and strain were calculated as 0.00155 T + 2.90 × 10?6 ε and 3.59 × 10?5 ε + 0.01887 T, respectively. Using the estimation of expanded uncertainty at 95% confidence level with a coverage factor of k = 2.205, temperature and strain measurement uncertainties were evaluated as 2.60 °C and 32.05 με, respectively. For the first time, to our knowledge, we have demonstrated the feasibility of estimating the measurement uncertainty for simultaneous strain-temperature sensing with such a fiber grating sensor.
An Optical Fiber Viscometer Based on Long-Period Fiber Grating Technology and Capillary Tube Mechanism
Jian-Neng Wang,Jaw-Luen Tang
Sensors , 2010, DOI: 10.3390/s101211174
Abstract: This work addresses the development and assessment of a fiber optical viscometer using a simple and low-cost long-period fiber grating (LPFG) level sensor and a capillary tube mechanism. Previous studies of optical viscosity sensors were conducted by using different optical sensing methods. The proposed optical viscometer consists of an LPFG sensor, a temperature-controlled chamber, and a cone-shaped reservoir where gravitational force could cause fluid to flow through the capillary tube. We focused on the use of LPFGs as level sensors and the wavelength shifts were not used to quantify the viscosity values of asphalt binders. When the LPFG sensor was immersed in the constant volume (100 mL) AC-20 asphalt binder, a wavelength shift was observed and acquired using LabVIEW software and GPIB controller. The time spent between empty and 100 mL was calculated to determine the discharge time. We simultaneously measured the LPFG-induced discharge time and the transmission spectra both in hot air and AC-20 asphalt binder at five different temperatures, 60, 80, 100, 135, and 170 Celsius. An electromechanical rotational viscometer was also used to measure the viscosities, 0.15–213.80 Pa·s, of the same asphalt binder at the above five temperatures. A non-linear regression analysis was performed to convert LPFG-induced discharge time into viscosities. Comparative analysis shows that the LPFG-induced discharge time agreed well with the viscosities obtained from the rotational viscometer.
Feasibility of Fiber Bragg Grating and Long-Period Fiber Grating Sensors under Different Environmental Conditions
Jian-Neng Wang,Jaw-Luen Tang
Sensors , 2010, DOI: 10.3390/s101110105
Abstract: This paper presents the feasibility of utilizing fiber Bragg grating (FBG) and long-period fiber grating (LPFG) sensors for nondestructive evaluation (NDE) of infrastructures using Portland cement concretes and asphalt mixtures for temperature, strain, and liquid-level monitoring. The use of hybrid FBG and LPFG sensors is aimed at utilizing the advantages of two kinds of fiber grating to implement NDE for monitoring strains or displacements, temperatures, and water-levels of infrastructures such as bridges, pavements, or reservoirs for under different environmental conditions. Temperature fluctuation and stability tests were examined using FBG and LPFG sensors bonded on the surface of asphalt and concrete specimens. Random walk coefficient (RWC) and bias stability (BS) were used for the first time to indicate the stability performance of fiber grating sensors. The random walk coefficients of temperature variations between FBG (or LPFG) sensor and a thermocouple were found in the range of ?0.7499 °C/?to ?1.3548 °C/. In addition, the bias stability for temperature variations, during the fluctuation and stability tests with FBG (or LPFG) sensors were within the range of 0.01 °C/h with a 15–18 h time cluster to 0.09 °C/h with a 3–4 h time cluster. This shows that the performance of FBG or LPFG sensors is comparable with that of conventional high-resolution thermocouple sensors under rugged conditions. The strain measurement for infrastructure materials was conducted using a packaged FBG sensor bonded on the surface of an asphalt specimen under indirect tensile loading conditions. A finite element modeling (FEM) was applied to compare experimental results of indirect tensile FBG strain measurements. For a comparative analysis between experiment and simulation, the FEM numerical results agreed with those from FBG strain measurements. The results of the liquid-level sensing tests show the LPFG-based sensor could discriminate five stationary liquid-levels and exhibits at least 1,050-mm liquid-level measurement capacity. Thus, the hybrid FBG and LPFG sensors reported here could benefit the NDE development and applications for infrastructure health monitoring such as strain, temperature and liquid-level measurements.
Photonic Crystal Fiber Mach-Zehnder Interferometer for Refractive Index Sensing
Jian-Neng Wang,Jaw-Luen Tang
Sensors , 2012, DOI: 10.3390/s120302983
Abstract: We report on a refractive index sensor using a photonic crystal fiber (PCF) interferometer which was realized by fusion splicing a short section of PCF (Blaze Photonics, LMA-10) between two standard single mode fibers. The fully collapsed air holes of the PCF at the spice regions allow the coupling of PCF core and cladding modes that makes a Mach-Zehnder interferometer. The transmission spectrum exhibits sinusoidal interference pattern which shifts differently when the cladding/core surface of the PCF is immersed with different RI of the surrounding medium. Experimental results using wavelength-shift interrogation for sensing different concentrations of sucrose solution show that a resolution of 1.62 × 10?4–8.88 × 10?4 RIU or 1.02 × 10?4–9.04 × 10?4 RIU (sensing length for 3.50 or 5.00 cm, respectively) was achieved for refractive indices in the range of 1.333 to 1.422, suggesting that the PCF interferometer are attractive for chemical, biological, biochemical sensing with aqueous solutions, as well as for civil engineering and environmental monitoring applications.
Chemical Sensing Sensitivity of Long-Period Grating Sensor Enhanced by Colloidal Gold Nanoparticles
Jaw-Luen Tang,Jien-Neng Wang
Sensors , 2008,
Abstract: A simple and effective method is proposed to improve spectral sensitivity anddetection limit of long period gratings for refractive index or chemical sensing, where thegrating surface is modified by a monolayer of colloidal gold nanoparticles. Thetransmission spectra and optical properties of gold nanospheres vary with the differentrefractive index of the environment near the surface of gold nanospheres. The sensorresponse of gold colloids increases linearly with solvents of increasing refractive index.The results for the measurement of sucrose and sodium chloride solutions are reported,which show that this type of sensor can provide a limiting resolution of ~10-3 to ~10-4 forrefractive indices in the range of 1.34 to 1.39 and a noticeable increase in detection limit ofrefractive index to external medium.
Formation of Tunable, Emulsion Micro-Droplets Utilizing Flow-Focusing Channels and a Normally-Closed Micro-Valve
Jung-Hao Wang,Gwo-Bin Lee
Micromachines , 2013, DOI: 10.3390/mi4030306
Abstract: A mono-dispersed emulsion is of great significance in many chemical, biomedical and industrial applications. The current study reports a new microfluidic chip capable of forming tunable micro-droplets in liquids for emulsification applications. It can precisely generate size-tunable, uniform droplets using flow-focusing channels and a normally-closed valve, which is opened by a pneumatic suction force. Experimental data showed that micro-droplets with a diameter ranging from several to tens of micrometers could be precisely generated with a high uniformity. The droplet size is experimentally found to be dependent on the velocity of the dispersed-phase liquid, which is controlled by the deflection of the suction membrane. Emulsions with droplet sizes ranging from 5.5 to 55 μm are successfully observed. The variation in droplet sizes is from 3.8% to 2.5%. The micro-droplets have a uniform size and droplets smaller than those reported in previous studies are possible with this approach. This new microfluidic device can be promising for emulsification and other related applications.
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