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Search Results: 1 - 10 of 32856 matches for " Guo CQ "
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Biocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells
Chen DZ, Tang QS, Li XD, Zhou XJ, Zang J, Xue WQ, Xiang JY, Guo CQ
International Journal of Nanomedicine , 2012, DOI: http://dx.doi.org/10.2147/IJN.S35140
Abstract: iocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells Original Research (2298) Total Article Views Authors: Chen DZ, Tang QS, Li XD, Zhou XJ, Zang J, Xue WQ, Xiang JY, Guo CQ Published Date September 2012 Volume 2012:7 Pages 4973 - 4982 DOI: http://dx.doi.org/10.2147/IJN.S35140 Received: 19 June 2012 Accepted: 01 August 2012 Published: 14 September 2012 Daozhen Chen,1,3,* Qiusha Tang,2,* Xiangdong Li,3,* Xiaojin Zhou,1 Jia Zang,1 Wen-qun Xue,1 Jing-ying Xiang,1 Cai-qin Guo1 1Central Laboratory, Wuxi Hospital for Matemaland Child Health Care Affiliated Medical School of Nanjing, Jiangsu Province; 2Department of Pathology and Pathophysiology, Medical College, Southeast University, Jiangsu Province; 3The People’s Hospital of Aheqi County, Xinjiang, China *These authors contributed equally to this work Background: The objective of this study was to evaluate the synthesis and biocompatibility of Fe3O4 nanoparticles and investigate their therapeutic effects when combined with magnetic fluid hyperthermia on cultured MCF-7 cancer cells. Methods: Magnetic Fe3O4 nanoparticles were prepared using a coprecipitation method. The appearance, structure, phase composition, functional groups, surface charge, magnetic susceptibility, and release in vitro were characterized by transmission electron microscopy, x-ray diffraction, scanning electron microscopy-energy dispersive x-ray spectroscopy, and a vibrating sample magnetometer. Blood toxicity, in vitro toxicity, and genotoxicity were investigated. Therapeutic effects were evaluated by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] and flow cytometry assays. Results: Transmission electron microscopy revealed that the shapes of the Fe3O4 nanoparticles were approximately spherical, with diameters of about 26.1 ± 5.2 nm. Only the spinel phase was indicated in a comparison of the x-ray diffraction data with Joint Corporation of Powder Diffraction Standards (JCPDS) X-ray powder diffraction files. The O-to-Fe ratio of the Fe3O4 was determined by scanning electron microscopy-energy dispersive x-ray spectroscopy elemental analysis, and approximated pure Fe3O4. The vibrating sample magnetometer hysteresis loop suggested that the Fe3O4 nanoparticles were superparamagnetic at room temperature. MTT experiments showed that the toxicity of the material in mouse fibroblast (L-929) cell lines was between Grade 0 to Grade 1, and that the material lacked hemolysis activity. The acute toxicity (LD50) was 8.39 g/kg. Micronucleus testing showed no genotoxic effects. Pathomorphology and blood biochemistry testing demonstrated that the Fe3O4 nanoparticles had no effect on the main organs and blood biochemistry in a rabbit model. MTT and flow cytometry assays revealed that Fe3O4 nano magnetofluid thermotherapy inhibited MCF-7 cell proliferation, and its inhibitory effect was dose-dependent according to the Fe3O4 nano magnetofluid concentration. Conclusion: The Fe3O4 nanopart
Preparation and biodistribution of 188Re-labeled folate conjugated human serum albumin magnetic cisplatin nanoparticles (188Re-folate-CDDP/HSA MNPs) in vivo
Tang QS, Chen DZ, Xue WQ, Xiang JY, Gong YC, Zhang L, Guo CQ
International Journal of Nanomedicine , 2011, DOI: http://dx.doi.org/10.2147/IJN.S24322
Abstract: eparation and biodistribution of 188Re-labeled folate conjugated human serum albumin magnetic cisplatin nanoparticles (188Re-folate-CDDP/HSA MNPs) in vivo Original Research (3306) Total Article Views Authors: Tang QS, Chen DZ, Xue WQ, Xiang JY, Gong YC, Zhang L, Guo CQ Published Date November 2011 Volume 2011:6 Pages 3077 - 3085 DOI: http://dx.doi.org/10.2147/IJN.S24322 Qiu-Sha Tang1,*, Dao-Zhen Chen2,*, Wen-Qun Xue2, Jing-Ying Xiang2, Yong-Chi Gong1, Li Zhang2, Cai-Qin Guo2 1Department of Pathology and Pathophysiology, Medical College, Southeast University, Nanjing, Jiangsu; 2Central Laboratory, Wuxi Hospital for Maternal and Child Health Care, Affiliated Medical School of Nanjin, Wuxi, Jiangsu, China *Authors contributed equally to this work Background: The purpose of this study was to develop intraperitoneal hyperthermic therapy based on magnetic fluid hyperthermia, nanoparticle-wrapped cisplatin chemotherapy, and magnetic particles of albumin. In addition, to combine the multiple-killing effects of hyperthermal targeting therapy, chemotherapy, and radiotherapy, the albumin-nanoparticle surfaces were linked with radionuclide 188Re-labeled folic acid ligand (188Re-folate-CDDP/HSA). Methods: Human serum albumin was labeled with 188Re using the pre-tin method. Reaction time and optimal conditions of labeling were investigated. The particles were intravenously injected into mice, which were sacrificed at different time points. Radioactivity per gram of tissue of percent injected dose (% ID/g) was measured in vital organs. The biodistribution of 188Re-folate-CDDP/HAS magnetic nanoparticles was assessed. Results: Optimal conditions for 188Re-labeled folate-conjugated albumin combined with cisplatin magnetic nanoparticles were: 0.1 mL of sodium gluconate solution (0.3 mol/L), 0.1 mL of concentrated hydrochloric acid with dissolved stannous chloride (10 mg/mL), 0.04 mL of acetic acid buffer solution (pH 5, 0.2 mol/L), 30 mg of folate-conjugated albumin combined with cisplatin magnetic nanoparticles, and 188ReO4 eluent (0.1 mL). The rate of 188Re-folate-CDDP-HSA magnetic nanoparticle formation exceeded 90%, and radiochemical purity exceeded 95%. The overall labeling rate was 83% in calf serum at 37°C. The major uptake tissues were the liver, kidney, intestine, and tumor after the 188Re-folate-CDDP/HSA magnetic nanoparticles were injected into nude mice. Uptake of 188Re-folate-CDDP/HSA magnetic nanoparticles increased gradually after injection, peaked at 8 hours with a value of 8.83 ± 1.71, and slowly decreased over 24 hours in vivo. Conclusion: These results indicate that 188Re-folate-CDDP/HSA magnetic nanoparticles can be used in radionuclide-targeted cancer therapy. Surface-modified albumin nanoparticles with folic acid ligand-labeled radionuclide (188Re) were successfully prepared, laying the foundation for a triple-killing effect of thermotherapy, chemotherapy, and radiation therapy.
CQ Xu,YWTian,YCZhai,ZJ Guo,ZQ Huang,
C.Q. Xu
,Y.W. Tian,Y.C. Zhai,Z.J. Guo and Z.Q. HuangSchool of Materials and Metallurgy,Northeastern University,Shenyang,China

金属学报(英文版) , 2005,
Abstract: The differential thermal analysis (DTA) curves were measured at different heating rates in flow-ing air for studying the synthesis of the spinel LiMn2O4 with Li2CO3 and MnO2. The reaction be-gan at about 503K, and finishedatabout 873K. The apparentactivation energy of Kissinger method(1-δ)1.67Coats-Redfern integral method was used to analyze the DTA curves of the samples at different heating rates, and the calculated apparent activation energy and frequency factor were diffraction (XRD) and scanning electron microscope (SEM) results shown that the synthesized LiMn2O4 possesses pure phase, regular shape and normal particle distribution.
In Situ Observation of Growth Behavior and Morphology of Delta-Ferrite as Function of Solidification rate in an AISI304 Stainless Steel

GF Liang,CQ Wan,JC Wu,

金属学报(英文版) , 2006,
Childhood meat eating and inflammatory markers: The Guangzhou Biobank Cohort Study
CM Schooling, CQ Jiang, TH Lam, WS Zhang, KK Cheng, GM Leung
BMC Public Health , 2011, DOI: 10.1186/1471-2458-11-345
Abstract: We used multivariable linear regression in the Guangzhou Biobank Cohort Study phase 3 (2006-8) to examine the adjusted associations of recalled childhood meat eating, <1/week (n = 5,023), about once per week (n = 3,592) and almost daily (n = 1,252), with white blood cell count and its differentials among older (≥50 years) men (n = 2,498) and women (n = 7,369).Adjusted for age, childhood socio-economic position, education and smoking, childhood meat eating had sex-specific associations with white blood cell count and lymphocyte count, but not granulocyte count. Men with childhood meat eating almost daily compared to <1/week had higher white blood cell count (0.33 109/L, 95% confidence interval (CI) 0.10 to 0.56) and higher lymphocyte count (0.16 109/L, 95% CI 0.07 to 0.25). Adjustment for obesity slightly attenuated these associations.If confirmed, this hypothesis implies that economic development and the associated improvements in nutrition at puberty may be less beneficial among men than women; consistent with the widening sex differentials in life expectancy with economic development.In long term industrialized western populations poor childhood conditions are associated with cardiovascular disease [1], although the underlying biological pathway is unclear. Cardiovascular disease may have an inflammatory component [2,3]. Poor childhood conditions are also usually associated with a pro-inflammatory state [4-9]. It is increasingly clear that the social patterning of cardiovascular disease or its risk factors, both traditional and non-traditional, is not universal but epidemiologic stage specific [9-14]. There is often a more marked and longer lasting reversal of the usual social patterning among men than women [9-11] for reasons which have tended to be interpreted mainly in terms of contemporaneous risk factors that differ by sex [11].A factor that countered the generally protective effect of social advantage among men at the early stages of economic development wou
New insights about host response to smallpox using microarray data
Gustavo H Esteves, Ana CQ Simoes, Estevao Souza, Rodrigo A Dias, Raydonal Ospina, Thiago M Venancio
BMC Systems Biology , 2007, DOI: 10.1186/1752-0509-1-38
Abstract: We used KEGG pathways annotations to define groups of genes (or modules), and subsequently compared them to macaque survival times. This technique provided additional insights about the host response to this disease, such as increased expression of the cytokines and ECM receptors in the individuals with higher survival times. These results could indicate that these gene groups could influence an effective response from the host to smallpox.Macaques with higher survival times clearly express some specific pathways previously unidentified using regular gene-by-gene approaches. Our work also shows how third party analysis of public datasets can be important to support new hypotheses to relevant biological problems.Large scale gene expression analysis with microarray technology is expanding and generating a large amount of high quality, publicly available data. In the present work we analyzed a dataset derived from monkeys infected by smallpox, published by Rubins et al [1]. Smallpox is a lethal disease that was endemic in many parts of the world until eradicated by a massive immunization program developed by the World Health Organization. Its fatality rate was estimated to be 30%, and the survivors often had disfiguring scars [2].There are serious concerns about the use of smallpox as a bioweapon [3,4]. Recently, some health care workers were vaccinated by the UK government for the analysis of antibody responses [5]. Pox viruses display unique abilities to interfere with the host immune system, producing immune modulators [6] and there are at least 16 viral genes involved in combating the host immune response [7]. The original study's goal was to analyze the evolution of the gene expression of the peripheral blood cells of variola-infected monkeys, so as to clarify the biological processes associated with host-pathogen interactions [1].Among the important results was the absence of a TNF-α/NF-κB-activated transcriptional mechanism during systemic infection, which could
Measuring outcomes in allergic rhinitis: psychometric characteristics of a Spanish version of the congestion quantifier seven-item test (CQ7)
Antonio Valero, Joaquim Mullol, Michael Herdman, Maria-José Rosales, Spanish CQ7 Study Group
Health and Quality of Life Outcomes , 2011, DOI: 10.1186/1477-7525-9-14
Abstract: CQ7 was adapted from English following international guidelines. The instrument was validated in an observational, prospective study in allergic rhinitis patients with NC (N = 166) and a control group without NC (N = 35). Participants completed the CQ7, MOS sleep questionnaire, and a measure of psychological well-being (PGWBI). Clinical data included NC severity rating, acoustic rhinometry, and total symptom score (TSS). Internal consistency was assessed using Cronbach's alpha and test-retest reliability using the intraclass correlation coefficient (ICC). Construct validity was tested by examining correlations with other outcome measures and ability to discriminate between groups classified by NC severity. Sensitivity and specificity were assessed using Area under the Receiver Operating Curve (AUC) and responsiveness over time using effect sizes (ES).Cronbach's alpha for the CQ7 was 0.92, and the ICC was 0.81, indicating good reliability. CQ7 correlated most strongly with the TSS (r = 0.60, p < 0.01), the PGWBI general health dimension (r = 0.56, p < 0.01), and the MOS Sleep scale 'sleep short of breath' dimension (r = 0.49, p < 0.01). Correlations with acoustic rhinometry were generally low. The instrument discriminated well between NC severity groups (ES 0.33-2.07) and AUC was 0.93, indicating excellent sensitivity and specificity. The measure was responsive to change (ES = 1.1) in patients reporting improvement in NC.The Spanish version of the CQ7 is appropriate for detecting, measuring, and monitoring NC in allergic rhinitis patients.Nasal congestion (NC) has been described as one of the most troublesome symptoms for patients with allergic rhinitis (AR) and is associated with poorer sleep, mood, and productivity [1,2]. A new tool to measure patient experience of NC is the Congestion Quantifier Seven-Item test (CQ7) which was developed recently in the United States [3]. The CQ7 was originally developed as a screening tool to identify patients with NC potentially
Reverse LISS plating for intertrochanteric Hip Fractures in elderly patients
CQ Zhang, Y Sun, DX Jin, C Yao, SB Chen, BF Zeng
BMC Musculoskeletal Disorders , 2010, DOI: 10.1186/1471-2474-11-166
Abstract: We treated 28 elderly patients with a mean age of 82.3 years. According to the Evens classification, there were 2 Type I fractures, 2 Type II fractures, 3 Type III fractures, 13 Type IV fractures, 6 Type V fractures and 2 Type R fractures. All fractures were treated using the reverse LISS. Radiographic and clinical evidence of functional outcome and complications were evaluated.Mean perioperative blood loss was 92.4 milliliters (range 35 to 245 milliliters), and the mean postoperative hospital stay was 8.7 days (range 3 to 14 days).Complications included one minor wound hematoma. Radiographically, no collapses, screw cutouts, or head penetrations were seen. All surviving patients (28 of 28; 100 percent) had uneventful fracture healing with union achieved by six months in all patients.Use of the Reverse LISS plating for intertrochanteric hip fractures resulted in event-free fracture healing.Hip fractures are a leading cause of death and disability among the elderly. Approximately 50% of hip fractures are intertrochanteric fractures, a large percentage of which are unstable [1,2]. Treatment goals for this patient population include early rehabilitation, restoration of the anatomic alignment of the proximal part of the femur, and maintenance of the fracture reduction [3]. Different approaches have been used to solve this problem, including trochanteric osteotomy techniques, cementing, and different types of fixation devices. Despite improved techniques and devices, failure of fixation is still a problem in unstable intertrochanteric fractures[4].In recent years, the minimally invasive surgical techniques have led to a widespread use of many new implants [5,6]it has been shown that they can reduce operative complications and postoperative morbidity. As such, the present study evaluates the treatment of intertrochanteric hip fractures with the reverse LISS plating system.The present study was reviewed and approved by our institutional review board, and informed consent w
Gluteus maximus and semitendinosus activation during active prone hip extension exercises
Sakamoto, ACL;Teixeira-Salmela, LF;Rodrigues, de Paula F;Guimar?es, CQ;Faria, CDCM;
Brazilian Journal of Physical Therapy , 2009, DOI: 10.1590/S1413-35552009005000045
Abstract: background: gluteus maximus strengthening exercises are employed in clinical practice as options for the treatment of low back and sacroiliac disorders. however, no studies were found that investigated which were the best exercises to activate this muscle and justify its employment in physical therapy practice. objective: to quantify the electromyographic (emg) activity of the gluteus maximus and semitendinosus muscles during four modalities of therapeutic exercises. methods: thirty-one participants (16 men, 15 women) were selected. the emg activities of the gluteus maximus and semitendinosus was recorded and quantified while the participants performed four modalities of therapeutic exercises, involving active prone hip extension in four positions: knee extension (ke), knee flexion (kf), lateral hip rotation and knee extension (lhrke), and lateral hip rotation and knee flexion (lhr-kf). results: repeated-measures anovas showed that the addition of kf or lhr increased gluteus maximus activity, whereas, ke decreased the activation of this muscle. in contrast, the exercises performed with ke increased semitendinosus activity. conclusions: exercises performed with kf or lhr, or a combination of the two, may be effective choices for gluteus maximus strengthening, however both kf and lhr decreased semitendinosus activity. the exercises performed with ke appeared to be an acceptable choice for semitendinosus activation.
Fatores associados à ades?o ao uso de palmilhas biomecanicas
Guimar?es, CQ;Teixeira-Salmela, LF;Rocha, IC;Bicalho, LI;Sabino, GS;
Brazilian Journal of Physical Therapy , 2006, DOI: 10.1590/S1413-35552006000300004
Abstract: background: the benefits of biomechanical insoles for treating lower limb impairment have been reported. however, in clinical practice, low compliance with the use of such orthoses has been observed, which detracts from the possible benefits. objective: to investigate the factors related to compliance with biomechanical insole use. method: thirty-three subjects (aged 44 ± 14.7 years) for whom biomechanical insoles had been molded took part in this study. a questionnaire asking about the insoles and their use was applied through interviews. the study variables were established from these questions. based on the results obtained, the participants were divided into four groups according to their compliance level: full compliance, partial compliance, irregular compliance and non-compliance. the kruskal-wallis test was utilized to assess differences between these groups regarding the variables investigated. spearman correlation coefficients were utilized to investigate possible relationships between these variables and the compliance. results: among the 33 participants, 15.2% were fully compliant, while 42.4% abandoned the insoles. comparing the four compliance groups in relation to the variables investigated, significant differences were observed regarding insole comfort (p=0.003), improvement attributed to the insoles (p=0.006), and degree of knowledge of the problem (p<0.002). significant correlations (rs=0.58; p<0.001; and rs=0.50; p<0.01) were observed between compliance and the comfort and improvement attributed to the insoles, respectively. conclusion: the findings from this study revealed that the comfort and degree of improvement attributed to the insoles may influence the compliance with the use of these orthoses. these factors are important for the success of the treatment.
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