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Search Results: 1 - 10 of 221053 matches for " Guillermo López-Campos "
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Characterization of Plasmids in a Human Clinical Strain of Lactococcus garvieae
Mónica Aguado-Urda, Alicia Gibello, M. Mar Blanco, Guillermo H. López-Campos, M. Teresa Cutuli, José F. Fernández-Garayzábal
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040119
Abstract: The present work describes the molecular characterization of five circular plasmids found in the human clinical strain Lactococcus garvieae 21881. The plasmids were designated pGL1-pGL5, with molecular sizes of 4,536 bp, 4,572 bp, 12,948 bp, 14,006 bp and 68,798 bp, respectively. Based on detailed sequence analysis, some of these plasmids appear to be mosaics composed of DNA obtained by modular exchange between different species of lactic acid bacteria. Based on sequence data and the derived presence of certain genes and proteins, the plasmid pGL2 appears to replicate via a rolling-circle mechanism, while the other four plasmids appear to belong to the group of lactococcal theta-type replicons. The plasmids pGL1, pGL2 and pGL5 encode putative proteins related with bacteriocin synthesis and bacteriocin secretion and immunity. The plasmid pGL5 harbors genes (txn, orf5 and orf25) encoding proteins that could be considered putative virulence factors. The gene txn encodes a protein with an enzymatic domain corresponding to the family actin-ADP-ribosyltransferases toxins, which are known to play a key role in pathogenesis of a variety of bacterial pathogens. The genes orf5 and orf25 encode two putative surface proteins containing the cell wall-sorting motif LPXTG, with mucin-binding and collagen-binding protein domains, respectively. These proteins could be involved in the adherence of L. garvieae to mucus from the intestine, facilitating further interaction with intestinal epithelial cells and to collagenous tissues such as the collagen-rich heart valves. To our knowledge, this is the first report on the characterization of plasmids in a human clinical strain of this pathogen.
Global Transcriptome Analysis of Lactococcus garvieae Strains in Response to Temperature
Mónica Aguado-Urda, Alicia Gibello, M. del Mar Blanco, José F. Fernández-Garayzábal, Victoria López-Alonso, Guillermo H. López-Campos
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0079692
Abstract: Lactococcus garvieae is an important fish and an opportunistic human pathogen. The genomic sequences of several L. garvieae strains have been recently published, opening the possibility of global studies on the biology of this pathogen. In this study, a whole genome DNA microarray of two strains of L. garvieae was designed and validated. This DNA microarray was used to investigate the effects of growth temperature (18°C and 37°C) on the transcriptome of two clinical strains of L. garvieae that were isolated from fish (Lg8831) and from a human case of septicemia (Lg21881). The transcriptome profiles evidenced a strain-specific response to temperature, which was more evident at 18°C. Among the most significant findings, Lg8831 was found to up-regulate at 18°C several genes encoding different cold-shock and cold-induced proteins involved in an efficient adaptive response of this strain to low-temperature conditions. Another relevant result was the description, for the first time, of respiratory metabolism in L. garvieae, whose gene expression regulation was temperature-dependent in Lg21881. This study provides new insights about how environmental factors such as temperature can affect L. garvieae gene expression. These data could improve our understanding of the regulatory networks and adaptive biology of this important pathogen.
Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
Mónica Aguado-Urda, Guillermo H López-Campos, José F Fernández-Garayzábal, Fernando Martín-Sánchez, Alicia Gibello, Lucas Domínguez, María M Blanco
BMC Microbiology , 2010, DOI: 10.1186/1471-2180-10-79
Abstract: The combination and integration of in silico analyses and in vitro CGH experiments, performed in comparison with the reference microorganisms, allowed establishment of an inter-species hybridization framework with a detection threshold based on a sequence similarity of ≥ 70%. With this threshold value, 267 genes were identified as having an analogue in L. garvieae, most of which (n = 258) have been documented for the first time in this pathogen. Most of the genes are related to ribosomal, sugar metabolism or energy conversion systems. Some of the identified genes, such as als and mycA, could be involved in the pathogenesis of L. garvieae infections.In this study, we identified 267 genes that were potentially present in L. garvieae CECT 4531. Some of the identified genes could be involved in the pathogenesis of L. garvieae infections. These results provide the first insight into the genome content of L. garvieae.Lactococcus garvieae is one of the most important bacterial pathogens that affect different farmed fish species in many countries, although its major impact is on the trout farm industry [1,2]. In addition to farmed fish, this microorganism has also been isolated from a wide range of wild fish species, from both fresh and marine water, as well as from giant fresh water prawns [3] and from wild marine mammals [4]. The host range of L. garvieae is not limited to aquatic species. This agent has also been identified in cows and water buffalos with subclinical mastitis [5,6] and from cat and dog tonsils [7]. In humans it has been isolated from the urinary tract, blood, and skin and from patients with pneumonia, endocarditis or septicaemia [8-11]. Recently, intestinal disorders in humans have been associated with the consumption of raw fish contaminated with this pathogen [12], which suggests that L. garvieae could be considered as a potentially zoonotic bacterium [3,12]. Despite the widespread distribution and emerging clinical significance of L. garvieae in both
A Comparative Study on Efficacy of Fludrocortisones versus Glucocorticoids and Vasodilators in the Treatment of Idiopathic Sensorineural Cochlear Hearing Loss  [PDF]
Daniel López-Campos, Daniel López-Aguado, Eugenia M. Campos-Ba?ales, José Luis de Serdio-Arias, Mar García-Sáinz
International Journal of Otolaryngology and Head & Neck Surgery (IJOHNS) , 2015, DOI: 10.4236/ijohns.2015.42021
Abstract: Introduction and Objectives: The idiopathic sensorineural cochlear hearing loss is one of the most frequent human sensory deficits and there is no specific drug therapy for it. The possible hearing recovery is related with the reestablishment of normal ionic homeostasis of the endolymph controlled by the mineralocorticoid as could be demonstrate experimentally. The purpose of this clinical trial was to confirm the efficacy of mineralocorticoids to the recovery of hearing level in patients suffering idiopathic sensorineural hearing loss (SNHL) against the glucocorticoids and vasodilator drugs. Material and Methods: The research lasted three months and involved 90 patients allocated into four different groups: Placebo group, consisted of 20 patients (10 men and 10 women); the group consisting of 22 patients treated with glucocorticoid therapy (12 men and 10 female); the group treated with mineralocorticoid therapy encompassed 26 patients (13 males and 13 females) and the group of vasodilators formed by 22 patients (12 men and 10 women). The level of hearing loss was estimated by the tests Liminal Tone Audiometry (LTA) and Auditory Brainstem Response (ABR). Results: The main features in this research were overall better response in improving the hearing level with the mineralocorticoid therapy. This improvement in hearing levels was greater in women than in men, and a higher response was found in the left ear regardless of patient’s gender. Conclusions: The hearing gain was significantly superior in the mineralocorticoids group followed by the glucocorticoids group whereas the response to vasodilators was lesser and with no statistical significance.
A method for automatically extracting infectious disease-related primers and probes from the literature
Miguel García-Remesal, Alejandro Cuevas, Victoria López-Alonso, Guillermo López-Campos, Guillermo de la Calle, Diana de la Iglesia, David Pérez-Rey, José Crespo, Fernando Martín-Sánchez, Víctor Maojo
BMC Bioinformatics , 2010, DOI: 10.1186/1471-2105-11-410
Abstract: We tested our approach using a test set composed of 297 manuscripts. The extracted sequences and their organism/gene annotations were manually evaluated by a panel of molecular biologists. The results of the evaluation show that our approach is suitable for automatically extracting DNA sequences, achieving precision/recall rates of 97.98% and 95.77%, respectively. In addition, 76.66% of the detected sequences were correctly annotated with their organism name. The system also provided correct gene-related information for 46.18% of the sequences assigned a correct organism name.We believe that the proposed method can facilitate routine tasks for biomedical researchers using molecular methods to diagnose and prescribe different infectious diseases. In addition, the proposed method can be expanded to detect and extract other biological sequences from the literature. The extracted information can also be used to readily update available primer/probe databases or to create new databases from scratch.Molecular technologies are used in routine clinical practice to identify microorganisms, and evaluate the presence of virulence factors, antibiotic resistance determinants and host-microbe interactions [1]. For instance, numerous nucleic acid assays have been developed [2] using hybridization or DNA extension techniques that include a wide range of technologies, such as polymerase chain reaction (PCR) methods [3], gene and whole genome sequencing [4,5], Luminex [6] and microarray analysis [7].There is a wide range of technologies that provide specific short base sequences of DNA as probes — used to detect the complementary base sequence of interest—or as primers—that guide the DNA amplification process—used for different purposes. Primers and probes are the main components of nucleic acid-based detection systems and have been the subject of multiple studies. Therefore, different software programs have been developed to design these specific sequences of primers and probes mini
Recría de terneras parda de monta?a: alimentación y desarrollo productivo
Bodas,R.; López-Campos,O.; Mantecón,A.R.;
Archivos de Zootecnia , 2009, DOI: 10.4321/S0004-05922009000200020
Abstract: an experiment was conducted to study the effect of level of nutrition on development and reproductive performance of replacement beef heifers. 108 parda de monta?a heifers (9 months initial age) were allocated to 3 groups: low (grass hay ad libitum), medium (grass hay ad libitum + 3 kg concentrate/animal and day) and high (grass hay and concentrate ad libitum) level of nutrition. body weight was recorded along experimental period. after 9 months, heifers were inseminated and all the groups received the same diet. heifers and their calves were weighed after parturition on two calving seasons. groups receiving concentrate showed greater weight (p<0.05) and average daily gain (p<0.05). however, these changes did not cause affect fecundity, calving interval and calves' birth weight (p>0.1).
Intubated patients developing tracheobronchitis or pneumonia have distinctive complement system gene expression signatures in the pre-infection period: A pilot study Los pacientes intubados que presentan traqueobronquitis o neumonía tienen patrones distintivos de expresión genética del sistema del complemento en el período previo a la infección: un estudio piloto
I. Martín-Loeches,E. Papiol,R. Almansa,G. López-Campos
Medicina Intensiva , 2012,
Abstract: Introduction: It remains unknown why some intubated patients remain infection-free while others develop tracheobronchitis (VAT) or pneumonia (VAP). Objective: To identify and compare VAP/VAT gene expression "signatures" using genome-wide oligonucleotide microarrays. Material and methods: A prospective translational study of gene expression profiles of VAP and VAT groups was carried out, establishing comparisons in both pre-infection and infection phases. Pathway and functional analyses were performed with Ingenuity Pathway Analysis (IPA). Data analysis and hierarchical clustering of the genes involved in the signalling pathways expressed differentially in the two groups were performed with GeneSpring GX 11.0. Results: Eight patients developing respiratory infections (3 VAP and 5 VAT) after 4 days of mechanical ventilation were assessed. Comparison of gene expression profiles in the pre-infection period revealed 5595 genes expressed differentially between VAP and VAT (p<0.01, fold change >2). Comparative IPA analysis identified a significant depression of the complement system signalling pathway in the VAP group, affecting the classical pathway along with the final common pathway (p<0.05). In addition, the cAMP and calcium signalling pathways were also significantly depressed in the VAP group during the pre-infection phase also. Conclusion: Intubated patients complicated with pneumonia developed immune impairment in the pre-infection period, manifesting as a relatively lower expression of genes involved in the complement system that differed from patients developing tracheobronchitis. These findings suggest that a significant proportion of VAP episodes cannot be prevented, but might be treatable through pre-emptive therapy. Introducción: Seguimos sin saber por qué algunos pacientes intubados no sufren infecciones mientras que otros presentan traqueobronquitis (TAV) o neumonía (NAV). Objetivo: Identificar y comparar los patrones de la expresión genética de la NAV/TAV usando micromatrices multigénicas oligonucleotídicas. Material y métodos: Se realizó un estudio aplicado prospectivo de los patrones de la expresión genética de los grupos con NAV y TAV, estableciendo comparaciones tanto en la fase previa a la infección como en la fase infecciosa. Se realizaron análisis de vías y funcionales con Ingenuity Pathway (IPA). Los análisis de datos y el agrupamiento jerárquico de los genes implicados en las vías de se alización expresados de forma diferenciada en ambos grupos se realizaron con GeneSpring GX 11.0. Resultados: Se evaluaron ocho pacientes que presentaro
Greenhouse Gas Emissions from Calf- and Yearling-Fed Beef Production Systems, With and Without the Use of Growth Promotants
John Basarab,Vern Baron,óscar López-Campos,Jennifer Aalhus,Karen Haugen-Kozyra,Erasmus Okine
Animals , 2012, DOI: 10.3390/ani2020195
Abstract: A spring calving herd consisting of about 350 beef cows, 14–16 breeding bulls, 60 replacement heifers and 112 steers were used to compare the whole-farm GHG emissions among calf-fed vs. yearling-fed production systems with and without growth implants. Carbon footprint ranged from 11.63 to 13.22 kg CO 2e per kg live weight (19.87–22.52 kg CO 2e per kg carcass weight). Enteric CH 4 was the largest source of GHG emissions (53–54%), followed by manure N 2O (20–22%), cropping N 2O (11%), energy use CO 2 (9–9.5%), and manure CH 4 (4–6%). Beef cow accounted for 77% and 58% of the GHG emissions in the calf-fed and yearling-fed. Feeders accounted for the second highest GHG emissions (15% calf-fed; 35–36% yearling-fed). Implants reduced the carbon footprint by 4.9–5.1% compared with hormone-free. Calf-fed reduced the carbon footprint by 6.3–7.5% compared with yearling-fed. When expressed as kg CO 2e per kg carcass weight per year the carbon footprint of calf-fed production was 73.9–76.1% lower than yearling-fed production, and calf-fed implanted was 85% lower than hormone-free yearling-fed. Reducing GHG emissions from beef production may be accomplished by improving the feed efficiency of the cow herd, decreasing the days on low quality feeds, and reducing the age at harvest of youthful cattle.
Components of physical capacity in patients with chronic obstructive pulmonary disease: relationship with phenotypic expression
Eduardo Márquez-Martín, Pilar Cejudo Ramos, José Luis López-Campos, et al
International Journal of Chronic Obstructive Pulmonary Disease , 2011, DOI: http://dx.doi.org/10.2147/COPD.S16646
Abstract: mponents of physical capacity in patients with chronic obstructive pulmonary disease: relationship with phenotypic expression Original Research (4271) Total Article Views Authors: Eduardo Márquez-Martín, Pilar Cejudo Ramos, José Luis López-Campos, et al Published Date January 2011 Volume 2011:6 Pages 105 - 112 DOI: http://dx.doi.org/10.2147/COPD.S16646 Eduardo Márquez-Martín1, Pilar Cejudo Ramos1, José Luis López-Campos1, María del Pilar Serrano Gotarredona2, Silvia Navarro Herrero2, Rodrigo Tallón Aguilar1, Emilia Barrot Cortes1, Francisco Ortega Ruiz1 1Medical-Surgical Unit of Respiratory Diseases, University Hospital Virgen del Rocío, Seville, Spain; 2Radiodiagnostic Unit, University Hospital Virgen del Rocío, Seville, Spain Background: More accurate phenotyping of COPD is of great interest since it may have prognostic and therapeutic consequences. We attempted to explore the possible relationship between the extent of emphysema, as assessed by high-resolution computed tomography (HRCT), and COPD severity. We also included some study variables involving exercise tolerance evaluation and peripheral muscle strength (PMS) measurement. Methods: Sixty-four patients with COPD (mean age 64 ± 7 years) were enrolled in a prospective observational cross-sectional study. All patients underwent clinical and functional evaluations: assessment of dyspnea, body mass index (BMI), health status assessment, spirometry testing, and arterial blood gas analysis. The extent of emphysema was graded using HRCT. Functional capacity was evaluated by a cardiopulmonary maximal exercise testing (CPET), the shuttle walking test, and by estimation of PMS. Results: Half of the study patients had an emphysematous phenotype. There was a significant correlation between the score derived from analysis of HRCT images and BMI and respiratory functional parameters, as well as VO2 max (maximal oxygen uptake) and chest pull 1RM (1 rep max). Compared with subjects with a nonemphysematous phenotype, those with an emphysematous phenotype showed a lower BMI, a reduced PMS, and displayed a lower power at CPET. Significant differences in lung function tests were found for diffusing capacity and hyperinflation. No significant differences in quality of life were observed between the two study groups. Conclusions: Compared with subjects with a nonemphysematous phenotype, subjects with an emphysematous phenotype has a different profile in terms of BMI, lung function, PMS, and exercise capacity.
El desgaste profesional: ?un tema relevante para la formación de los docentes?
López-Campos, Celsa;Limones Aguilar, María de Lourdes;Valdés López, Aldo;Valdés-Perezgasga, Uriel;
En-claves del pensamiento , 2010,
Abstract: this work tries to find if high school teachers from a mexican institution feel they have a need to be trained in strategies that will let them handle teacher burnout situations. a concise revision of the terms "need" and "burnout" is included, followed by a methods section where the authors explain how they used the formative needs survey for high school teachers. this instrument was used to investigate if the teachers perceived they had a training need to know and implement strategies and methods to effectively face teacher burnout. the authors report statistically significant findings that point to the recognition by the teachers of a felt need of training in this field.
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