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Search Results: 1 - 10 of 28433 matches for " Guanlin;Sun "
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Characterization and comparison of Serratia marcescens isolated from edible cactus and from silkworm for virulence potential and chitosan susceptibility
Li, Bin;Yu, Rongrong;Liu, Baoping;Tang, Qiaomei;Zhang, Guoqing;Wang, Yanli;Xie, Guanlin;Sun, Guochang;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000100013
Abstract: representative strains of serratia marcescens from an edible cactus plant and silkworms were characterized and a comparison based on their cellular fatty acid composition, 16s rrna and groe gene sequence analysis as well as silkworm virulence and chitosan susceptibility was carried out. results from this study indicate that there are no significant differences between the phenotypic and molecular characterization, virulence and chitosan susceptibility of the s. marcescens strains from the cactus plant and silkworms. silkworms inoculated with s. marcescens from either plant or silkworm resulted in nearly 100% mortality. chitosan solution exhibited strong antibacterial activity against s. marcescens. this activity increased with the increase of chitosan concentration and incubation time regardless of the strain source. also, the results indicate that the plant associated s. marcescens maybe plays a possible role in the contamination of humans and animals, in particular silkworms, while chitosan showed a potential to control the contamination caused by s. marcescens.
Evolution of resistive switching polarity in Au/Ar+ bombarded SrTi0.993Nb0.007O3/In sandwiches
GuanLin Xie,YuHang Wang,TianLing Ren,JiaLin Zhu,JiaLin Sun,LiuWan Zhang
Chinese Science Bulletin , 2012, DOI: 10.1007/s11434-011-4859-3
Abstract: We investigated the resistive switching characteristics of Au/Ar+ bombarded SrTi0.993Nb0.007O3/In sandwiches. The evolution of the resistive switching polarity with sweeping voltage was observed. Our experiments showed that under a macroscopic electrode the homogeneous trapping-detrapping-type conduction and filament-type conduction coexist and compete with each other. For a large sweeping voltage range, the trapping-detrapping-type conduction dominates. However, for a small range the latter dominates. If the bias voltage is too large, the filament conduction could be destroyed. These results will help deepen the understanding of the resistive switching polarity, and will aid in future device design.
Phytoremediation and strengthening measures for soil contaminated by heavy metals

Sun Yuebing,Zhou Qixing,Guo Guanlin,

环境工程学报 , 2007,
Abstract: Phytoremediation has been regarded as a suitable technique for the pollution control of heavymetal contaminated soil for green, safe technology and the properties of efficiency, economy and ecological harmony. And it has been applied widely on the spot for polluted soil for its thorough remediation and environmentfriendly alternative. After a short introduction to the principles and types of phytormediation,this review focused on strengthening methods including biotechnology, agricultural technology, physical and chemical technology. Multi-channel approaches should be taken to make pbytoremediation and engineering available, field survey at more place to try to research for more tolerant and accumulating plants which may provide for the breakthrough of phytoremediation technique. This provided novel approaches to restore the heavy metal polluted soil using efficient remediation techniques. It offered technique route and scientific basis for commercial application and remediation of contaminated soil on a large scale.
New Measuring Method for Colorless Liquid Level Based on CLC100 Level Sensor and Powercast Wireless Sensor Kit  [PDF]
Guanlin Yan, Gerhard Lindner, Yuming Shen
Engineering (ENG) , 2015, DOI: 10.4236/eng.2015.74014
Abstract: In this paper, a new wireless measuring method for colorless liquid level measurement was presented, based on latest CLC100 liquid level sensor and Powercast wireless sensor development kit. The wireless system is divided into two parts, level measurement and data transmission part as well as data receiving and display part. First part included the capacitive liquid level sensor CLC100 and the wireless senor board. CLC100 sensor was used for liquid level measurement. Wireless sensor kit from Powercast Corporation included one wireless sensor board, which was used for signal transmission. A built-in PIC microcontroller was embedded in the transmission module, for the purpose of processing and data transmitting. Due to CLC100 sensor’s output voltage exceeding the sensor board’s input limitation, a voltage convertor was designed to connect the sensor and the wireless sensor board. The final results were voltages corresponding to the liquid level, and were processed by an independent PIC development board, and then sent to PC’s hyper terminal via serial-port by this PIC microcontroller. Experiments showed that this wireless sensor node prototype worked well.
Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima
Yanli Wang,Liping Li,Bin Li,Guoxing Wu,Qiaomei Tang,Muhammad Ibrahim,Hongye Li,Guanlin Xie,Guochang Sun
Molecules , 2012, DOI: 10.3390/molecules17067028
Abstract: The antibacterial activity and mechanism of two kinds of chitosan were investigated against twelve Xanthomonas strains recovered from Euphorbia pulcherrima. Results indicated that both chitosans markedly inhibited bacterial growth based on OD loss. Furthermore, the release of DNA and RNA from three selected strains was increased by both chitosans. However, the release of intracellular proteins was inhibited by both chitosans at different concentration and incubation times, except chitosan A at 0.1 mg/mL for 0.5 h incubation and 0.2 mg/mL for 2.0 h incubation increased the release of proteins, indicating the complexity of the interaction and cell membranes, which was affected by incubation time, bacterial species, chitosan type and concentration. Transmission electron microscopic observations revealed that chitosan caused changes in protoplast concentration and surface morphology. In some cells, the membranes and walls were badly distorted and disrupted, while other cells were enveloped by a thick and compact ribbon-like layer. The contrary influence on cell morphology may explain the differential effect in the release of material. In addition, scanning electron microscope and biofilm formation test revealed that both chitosans removed biofilm biomass. Overall, this study showed that membrane and biofilm play an important role in the antibacterial mechanism of chitosan.
Interkingdom Gene Transfer May Contribute to the Evolution of Phytopathogenicity in Botrytis Cinerea
Bo Zhu, Qing Zhou, Guanlin Xie, Guoqing Zhang, Xiaowei Zhang, Yanli Wang, Gunchang Sun, Bin Li and Gulei Jin
Evolutionary Bioinformatics , 2012, DOI: 10.4137/EBO.S8486
Abstract: The ascomycete Botrytis cinerea is a phytopathogenic fungus infecting and causing significant yield losses in a number of crops. The genome of B. cinerea has been fully sequenced while the importance of horizontal gene transfer (HGT) to extend the host range in plant pathogenic fungi has been recently appreciated. However, recent data confirm that the B. cinerea fungus shares conserved virulence factors with other fungal plant pathogens with narrow host range. Therefore, interkingdom HGT may contribute to the evolution of phytopathogenicity in B. cinerea. In this study, a stringent genome comparison pipeline was used to identify potential genes that have been obtained by B. cinerea but not by other fungi through interkingdom HGT. This search led to the identification of four genes: a UDP-glucosyltransferase (UGT), a lipoprotein and two alpha/beta hydrolase fold proteins. Phylogenetic analysis of the four genes suggests that B. cinerea acquired UGT from plants and the other 3 genes from bacteria. Based on the known gene functions and literature searching, a correlation between gene acquision and the evolution of pathogenicity in B. cinerea can be postulated.
Study on the Relation of Knowledge Alliance Synergy Innovation Performance and Its Influencing Factors

孙新波,张大鹏, 吴冠霖, 钱雨
SUN Xinbo
, ZHANG Dapeng, WU Guanlin, QIAN Yu

- , 2015,
Abstract: 摘要 协同创新是一个受多种因素综合影响的过程,在文献和相关理论分析的基础上,提出了知识联盟协同创新影响因素的概念模型并进行实证分析。研究表明,协同创新绩效由产品创新、管理创新和流程创新3个维度构成;联盟成员关系和协同创新资源对联盟协同创新绩效的3个维度都有显著正向影响;协同创新环境只对管理创新有显著影响,企业自身素质对协同创新绩效的3个维度都没有显著影响。
Collaborative innovation is a process affected by many factors. This study is relying on the basis of theoretical analysis and literature related on collaborative innovation knowledge alliance. We proposed the model explaining the factors that influence the collaborative innovation and to do the empirical analysis. The result shows that the collaborative innovation performance consists of three dimensions that are product innovation, management innovation and process innovation. The membership of the alliance has a positive effect on three dimensions of the collaborative innovation performance. The environment of collaborative innovation only influences the management innovation significantly. The own quality of the firms has no significant effect on three dimensions of the collaborative innovation performance
Influence of acetochlor on Pb forms and their bioavailability in phaiozem of northeast China

ZHOU Qixing,SUN Fuhong,GUO Guanlin,SUN Tieheng,

应用生态学报 , 2004,
Abstract: By using Pb-fractionation analytical methods and Pb-acetochlor response experiments,it was studied the Pb forms in clean and polluted phaiozems of northeast China and the response of Pb bioavailability to acetochlor application.The results showed that the dominant Pb forms in these soils were low bio-available organic-sulfide Pb and residual Pb,and the concentrations of high bio-available water-soluble and exchangeable Pb were relatively low.However,the ratio of bio-available Pb to total Pb was lower in polluted phaiozem than in clean phaiozem.The bioavailability of Pb in the soil was obviously increased after high dose of acetochlor was applied.The main reason was the contribution of organic-sulfide Pb to bio-available Pb interacted with acetochlor.
Differential Expression of In Vivo and In Vitro Protein Profile of Outer Membrane of Acidovorax avenae Subsp. avenae
Muhammad Ibrahim, Yu Shi, Hui Qiu, Bin Li, Amara Jabeen, Liping Li, He Liu, Michael Kube, Guanlin Xie, Yanli Wang, Guochang Sun
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0049657
Abstract: Outer membrane (OM) proteins play a significant role in bacterial pathogenesis. In this work, we examined and compared the expression of the OM proteins of the rice pathogen Acidovorax avenae subsp. avenae strain RS-1, a Gram-negative bacterium, both in an in vitro culture medium and in vivo rice plants. Global proteomic profiling of A. avenae subsp. avenae strain RS-1 comparing in vivo and in vitro conditions revealed the differential expression of proteins affecting the survival and pathogenicity of the rice pathogen in host plants. The shotgun proteomics analysis of OM proteins resulted in the identification of 97 proteins in vitro and 62 proteins in vivo by mass spectrometry. Among these OM proteins, there is a high number of porins, TonB-dependent receptors, lipoproteins of the NodT family, ABC transporters, flagellins, and proteins of unknown function expressed under both conditions. However, the major proteins such as phospholipase and OmpA domain containing proteins were expressed in vitro, while the proteins such as the surface anchored protein F, ATP-dependent Clp protease, OmpA and MotB domain containing proteins were expressed in vivo. This may indicate that these in vivo OM proteins have roles in the pathogenicity of A. avenae subsp. avenae strain RS-1. In addition, the LC-MS/MS identification of OmpA and MotB validated the in silico prediction of the existance of Type VI secretion system core components. To the best of our knowledge, this is the first study to reveal the in vitro and in vivo protein profiles, in combination with LC-MS/MS mass spectra, in silico OM proteome and in silico genome wide analysis, of pathogenicity or plant host required proteins of a plant pathogenic bacterium.
Inhibiting effect of melittin on pathogens of crops
LingZi Pan,Jie Na,Zhuo Xing,HongJun Fang,GuanLin Wang
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0117-0
Abstract: The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylinder-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens effectively, and bacteriostatic action was influenced by factors such as pH and temperature. We elucidated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These results suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency.
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