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Search Results: 1 - 10 of 27866 matches for " Guangpeng Ma "
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Research on Talent Incentive Mechanism in the Closed Type Healthcare Alliance  [PDF]
Jing Zhang, Mingmin Wang, Yahui Ma, Yaoyao Jia, Guangpeng Zhang
Open Access Library Journal (OALib Journal) , 2017, DOI: 10.4236/oalib.1104018
The construction and development of the closed type health care is an important measure to deepen the reform of the three medical entities, to rationally allocate medical and health resources, to enable the grassroots people to enjoy the high quality and convenient medical services, and for the efficient implementation of closed type medical alliance, the perfect incentive mechanism plays a particularly important role. Through combing the current situation of talent incentive mechanism in closed type health care alliance of China, this paper discusses the challenges in practice and puts forward some feasible suggestions in order to promote the further development of close type medical alliance.
Acute Respiratory Distress Syndrome Induced by a Swine 2009 H1N1 Variant in Mice
Yi Zhang, Honglei Sun, Lihong Fan, Yuan Ma, Yipeng Sun, Juan Pu, Jun Yang, Jian Qiao, Guangpeng Ma, Jinhua Liu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0029347
Abstract: Background Acute respiratory distress syndrome (ARDS) induced by pandemic 2009 H1N1 influenza virus has been widely reported and was considered the main cause of death in critically ill patients with 2009 H1N1 infection. However, no animal model has been developed for ARDS caused by infection with 2009 H1N1 virus. Here, we present a mouse model of ARDS induced by 2009 H1N1 virus. Methodology Principal Findings Mice were inoculated with A/swine/Shandong/731/2009 (SD/09), which was a 2009 H1N1 influenza variant with a G222D mutation in the hemagglutinin. Clinical symptoms were recorded every day. Lung injury was assessed by lung water content and histopathological observation. Arterial blood gas, leukocyte count in the bronchial alveolar lavage fluid and blood, virus titers, and cytokine levels in the lung were measured at various times post-inoculation. Mice infected with SD/09 virus showed typical ARDS symptoms characterized by 60% lethality on days 8–10 post-inoculation, highly edematous lungs, inflammatory cellular infiltration, alveolar and interstitial edema, lung hemorrhage, progressive and severe hypoxemia, and elevated levels of proinflammatory cytokines and chemokines. Conclusions/Significance These results suggested that we successfully established an ARDS mouse model induced by a virulent 2009 H1N1 variant without previous adaptation, which may be of benefit for evaluating the pathogenesis or therapy of human ARDS caused by 2009 H1N1 virus.
Expression and intracellular localization of duck enteritis virus pUL38 protein
Jun Xiang, Guangpeng Ma, Shunchuan Zhang, Anchun Cheng, Mingshu Wang, Dekang Zhu, Renyong Jia, Qihui Luo, Zhengli Chen, Xiaoyue Chen
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-162
Abstract: Duck enteritis virus (DEV) is a natural pathogen of ducks and causes duck viral enteritis, an acute, contagious, and lethal disease affecting waterfowl belonging to the family Anatidae [1]. DEV is a member of the family Herpesviridae. The DEV virion is enveloped, and the genome consists of double-stranded DNA segments packaged in an icosahedral capsid [2]. The gene library of the DEV CHv strain was constructed in our laboratory, and more than 72 major open reading frames (ORFs) were found [3], coding for enzymes, structural proteins, and scaffolding proteins. However, the functional characteristics of most of these proteins are still unknown. To date, only the kinetics of expression and intracellular location of pUL24 [4], pUL31 [5,6], pUL51 [7,8], pUS3 [9], and dUTPase [10] have been investigated. Using bioinformatic tools, some putative glycoproteins and enzymes of the virus were characterized, such as gC [11], gE [12], gI, gD [13], and helicase pUL5 [14]. The identity of other components remains obscure. The DEV pUL38 protein has been suggested to be a putative structural protein. Computational predictions have revealed that DEV pUL38 mainly targets the cytoplasm and nucleus [15]. Immunological assays are an essential part of studies aimed at determining the kinetics of expression and the cellular location of DEV pUL38 in vitro. In this study, we obtained rabbit anti-pUL38 polyclonal sera, which were shown to be functional in immunofluorescence and western blotting assays.The DEV CHv strain used throughout this study was grown in duck embryo fibroblast (DEF) cells. Cell cultures were maintained in modified Eagle's medium (MEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics [16]. In a previous study, we had amplified the ORF of pUL38 (1398 bp) from the DEV genome [15]. The amplified product was cloned between the BamHI and XhoI sites of a pET32(+) plasmid, and a pET32-pUL38 plasmid construct was created.Escherichia coli BL21(DE3) was transformed wi
Expressing gK gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis
Shunchuan Zhang, Guangpeng Ma, Jun Xiang, Anchun Cheng, Mingshu Wang, Dekang Zhu, Renyong Jia, Qihui Luo, Zhengli Chen, Xiaoyue Chen
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-168
Abstract: Bioinformatic predictions revealed that the expression of the full-length gK gene (fgK) in a prokaryotic system is difficult because of the presence of suboptimal exon and transmembrane domains at the C-terminal. In this study, we found that the fgK gene might not be expressed in a prokaryotic system in accordance with the bioinformatic predictions. Further, we successfully used bioinformatics tools to guide the prokaryotic expression of the gK gene by designing a novel truncated gK gene (tgK). These findings indicated that bioinformatics provides theoretical data for target gene expression and saves time for our research. The recombinant tgK protein (tgK) was expressed and purified by immobilized metal affinity chromatography (IMAC). Western blotting and indirect enzyme-linked immunosorbent assay (ELISA) showed that the tgK possessed antigenic characteristics similar to native DEV-gK.In this work, the DEV-tgK was expressed successfully in prokaryotic system for the first time, which will provide usefull information for prokaryotic expression of alphaherpesvirus gK homologs, and the recombinant truncated gK possessed antigenic characteristics similar to native DEV gK. Because of the good reactionogenicity, specificity and sensitivity, the purified tgK could be useful for developing a sensitive serum diagnostic kit to monitor DEV outbreaks.Duck viral enteritis is caused by the duck enteritis virus (DEV). DEV has been included in the subfamily Alphaherpesvirinae of the family Herpesviridae, but it has not been grouped into a genus [1]. DEV has an icosahedral capsid containing a double-stranded linear DNA with 64.3% G + C content, which is higher than that of any other reported avian herpesvirus in the subfamily Alphaherpesvirinae [2]. The nucleocapsid is surrounded by a tegument, which is enclosed by an envelope with integral viral glycoproteins [3].DEV causes an acute, contagious, and highly lethal disease in birds of all ages from the order Anseriformes (ducks, gees
Generalized Fuzzy Metric Spaces with Properties
Guangpeng Sun,Kai Yang
Research Journal of Applied Sciences, Engineering and Technology , 2010,
Abstract: In this study, the notion of Q-fuzzy metric space is introduced and some properties are obtained. Two new common fixed point theorem s are proved in Q-fuzzy metric spaces under some suitable conditions.
Characterization of an Artificial Swine-Origin Influenza Virus with the Same Gene Combination as H1N1/2009 Virus: A Genesis Clue of Pandemic Strain
Xueli Zhao,Yipeng Sun,Juan Pu,Lihong Fan,Weimin Shi,Yanxin Hu,Jun Yang,Qi Xu,Jingjing Wang,Dongjun Hou,Guangpeng Ma,Jinhua Liu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0022091
Abstract: Pandemic H1N1/2009 influenza virus, derived from a reassortment of avian, human, and swine influenza viruses, possesses a unique gene segment combination that had not been detected previously in animal and human populations. Whether such a gene combination could result in the pathogenicity and transmission as H1N1/2009 virus remains unclear. In the present study, we used reverse genetics to construct a reassortant virus (rH1N1) with the same gene combination as H1N1/2009 virus (NA and M genes from a Eurasian avian-like H1N1 swine virus and another six genes from a North American triple-reassortant H1N2 swine virus). Characterization of rH1N1 in mice showed that this virus had higher replicability and pathogenicity than those of the seasonal human H1N1 and Eurasian avian-like swine H1N1 viruses, but was similar to the H1N1/2009 and triple-reassortant H1N2 viruses. Experiments performed on guinea pigs showed that rH1N1 was not transmissible, whereas pandemic H1N1/2009 displayed efficient transmissibility. To further determine which gene segment played a key role in transmissibility, we constructed a series of reassortants derived from rH1N1 and H1N1/2009 viruses. Direct contact transmission studies demonstrated that the HA and NS genes contributed to the transmission of H1N1/2009 virus. Second, the HA gene of H1N1/2009 virus, when combined with the H1N1/2009 NA gene, conferred efficient contact transmission among guinea pigs. The present results reveal that not only gene segment reassortment but also amino acid mutation were needed for the generation of the pandemic influenza virus.
An Overall Orientation Method for the Regional Network of Laser Beam Block Point Cloud Based on Linear Fitting

姚吉利, 徐广鹏, 马宁, 贾象阳, 田鹏艳, 王江妹, 李彩林
YAO Jili
, XU Guangpeng, MA Ning, JIA Xiangyang, TIAN Pengyan, WANG Jiangmei, LI Cailin

- , 2016, DOI: 10.13203/j.whugis20140438
Abstract: 从三维坐标转换模型出发,根据罗德里格矩阵的性质,首先推导出了单标靶点云定向的线性拟合方程,再以同名激光束相交于公共标靶为约束条件建立线性约束方程,进而实现全区域所有扫描站点云定向参数的整体解算。相对于传统的非线性的单站点云定向方法,本文方法能对区域内多站点云整体定向,计算过程不需要计算参数初值,定向后各站点云精度的一致性较好
Genetic Differentiation and Delimitation between Ecologically Diverged Populus euphratica and P. pruinosa
Juan Wang, Yuxia Wu, Guangpeng Ren, Qiuhong Guo, Jianquan Liu, Martin Lascoux
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0026530
Abstract: Background The fixed genetic differences between ecologically divergent species were found to change greatly depending on the markers examined. With such species it is difficult to differentiate between shared ancestral polymorphisms and past introgressions between the diverging species. In order to disentangle these possibilities and provide a further case for DNA barcoding of plants, we examine genetic differentiation between two ecologically divergent poplar species, Populus euphratica Oliver and P. pruinosa Schrenk using three different types of genetic marker. Methodology/Principal Findings We genotyped 290 individuals from 29 allopatric and sympatric populations, using chloroplast (cp) DNA, nuclear (nr) ITS sequences and eight simple sequence repeat (SSR) loci. Three major cpDNA haplotypes were widely shared between the two species and between-species cpDNA differentiation (FCT) was very low, even lower than among single species populations. The average SSR FCT values were higher. Bayesian clustering analysis of all loci allowed a clear delineation of the two species. Gene flow, determined by examining all SSR loci, was obvious but only slightly asymmetrical. However, the two species were almost fixed for two different nrITS genotypes that had the highest FCT, although a few introgressed individuals were detected both in allopatric and sympatric populations. Conclusions The two species shared numerous ancestral polymorphisms at cpDNA and a few SSR loci. Both ITS and a combination of nuclear SSR data could be used to differentiate between the two species. Introgressions and gene flow were obvious between the two species either during or after their divergence. Our findings underscore the complex genetic differentiations between ecologically diverged species and highlight the importance of nuclear DNA (especially ITS) differentiation for delimiting closely related plant species.
Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate
Ruoyu Wang, Guangpeng Wang, Qi Zhao, Yu Zhang, Lizhe An, Yun Wang
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-34
Abstract: Thus, full-length cDNA of LSV coat protein was synthesized and amplified by RT-PCR from RNA isolated from LSV Lanzhou isolate. The extended 33.6 kDa CP was cloned and expressed prokaryoticly and then purified by Ni-ion affinity chromatography. Its identity and antigenicity of recombinant CP were identified on Western-blotting by using the prepared anti-LSV antibodies.The results indicate that fusion CP maintains its native antigenicity and specificity, providing a good source of antigen in preparation of LSV related antibodies. Detailed structural analysis of a pure recombinant CP should allow a better understanding of its role in cell attachment and LSV tropism. This investigation to LSV should provide some specific antibodies and aid to development a detection system for LSV diagnostics and epidemiologic surveys.Lanzhou lily (L. davidii Duch.var) is an important bulb edible crop which mostly distributes in middle area of Gansu province in China. Virus infection caused serious reduction in production of Lanzhou lily and other economic corps in recent years [1,2]. Lily symptomless virus (LSV; family, Genus Carlavirus, species) is the most prevalent virus infecting Lanzhou lily [2], and it has been reported in USA, Europe, Australia and Asia [3-7]. It is also one of the most harmful viruses of lilies that causes severe losses in terms of quantity as well as quality of bulb and flower production[8]. The host range of LSV is mostly distributed in genus Lilium, however, in one case reported in Alstroemeria[9]. The observed abnormalities such as growth reduction, smaller flowers and lower bulb yield can be caused by combined infection with LSV and cucumber mosaic virus (CMV) [8] which threatens the yield and commercial production of lily plants.LSV contains a filamentous viral particle, 640 nm in length and 17-18 nm in diameter. The genomic RNA of LSV is constituted of 8,394 nucleotides (excluding the poly (A) tail) and contains six open reading frames (ORFs) coding for

JIANG Jinquan,QIN Guangpeng,LIU Chuanxiao,

岩石力学与工程学报 , 2006,
Abstract: The roof and side of fully-mechanized caving roadway are coal body or coal pillar which cause the stability problem extremely prominent. The fully-mechanized caving roadway of Nantun Colliery is set as the prototype, its surrounding rock stability problem is analyzed by numerical simulation with distinct element method and the variable and time series that describe system dynamic state are collected. The chaos and dynamic feature of the surrounding rock system are studied based on the power spectrum analysis, extraction of fractal dimension and calculation of maximum Lyapunov exponent. The deformation mechanic process of fully-mechanized caving surrounding rock is chaos dynamic process which is sensitive to initial factors; the pillar width and support condition have great effect on surrounding rock displacement, stress and damage area. The power spectrum has the feature of continuation, noise background and wide peak, the correlation dimension value is fraction and maximum Lyapunov exponent is generally greater than zero when the pillar width less than 3 m; the system would be in a chaos motion state at present. When the pillar width is 3 - 4 m and after bolt support, the wide peak feature of power spectrum vanished and the frequency spectrum is not profuse; the correlation dimension value is fraction but maximum Lyapunov exponent changes from positive to negative; system state changes from chaos motion to normal motion; this status is an inflection point of system dynamic state transformation. When the pillar width larger than 5 m, system will be in normal motion state. The reasonable pillar width is 3 - 5 m. The chaos dynamic feature of fully-mechanized caving roadway can be evaluated according to the application of power spectrum analysis and maximum Lyapunov exponent method.
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