oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2020 ( 3 )

2019 ( 315 )

2018 ( 2225 )

2017 ( 2109 )

Custom range...

Search Results: 1 - 10 of 127093 matches for " Guangjian LI "
All listed articles are free for downloading (OA Articles)
Page 1 /127093
Display every page Item
Subcellular Distribution and Genotoxicity of Silica Nanoparticles in Human Bronchial Epithelial Cells
Guangqiang ZHAO,Yunchao HUANG,Guangjian LI,Sen LI
Chinese Journal of Lung Cancer , 2013, DOI: 10.3779/j.issn.1009-3419.2013.03.01
Abstract: Background and objective Silicon nanoparticles are widely used in daily life. Therefore, they attract increased attention because of their potential biotoxicity to the lungs when inhaled. The aims of this study are to explore the organism distribution and genotoxicity of silica nanoparticles in human bronchial epithelial cells (BEAS-2B). Methods The biodistribution of silica with different particle sizes in human bronchial epithelial cells was observed by transmission electron microscopy (TEM). DNA damage was detected by single-cell gel electrophoresis (comet assay). Results TEM revealed that SiO2 nanoparticles with different sizes can be uptaken by cells and be localized in the cytoplasm and the nucleus. Compared with micro-silica, nano-silica in BEAS-2B cells can inflict more severe DNA damage (P<0.05). Conclusion The particle size of silica nanoparticles can be used to determine their distribution in biological cells. Compared with micro-silica, nano-silica has higher genotoxicity.
Mapping Paratope on Antithrombotic Antibody 6B4 to Epitope on Platelet Glycoprotein Ibalpha via Molecular Dynamic Simulations
Xiang Fang,Ying Fang,Li Liu,Guangjian Liu,Jianhua Wu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0042263
Abstract: Binding of platelet receptor glycoprotein Ibα (GPIbα) to the A1 domain of von Willebrand factor (vWF) is a critical step in both physiologic hemostasis and pathologic thrombosis, for initiating platelet adhesion to subendothelium of blood vessels at sites of vascular injury. Gain-of-function mutations in GPIbα contribute to an abnormally high-affinity binding of platelets to vWF and can lead to thrombosis, an accurate complication causing heart attack and stroke. Of various antithrombotic monoclonal antibodies (mAbs) targeting human GPIbα, 6B4 is a potent one to inhibit the interaction between GPIbα and vWF-A1 under static and flow conditions. Mapping paratope to epitope with mutagenesis experiments, a traditional route in researches of these antithrombotic mAbs, is usually expensive and time-consuming. Here, we suggested a novel computational procedure, which combines with homology modeling, rigid body docking, free and steered molecular dynamics (MD) simulations, to identify key paratope residues on 6B4 and their partners on GPIbα, with hypothesis that the stable hydrogen bonds and salt bridges are the important linkers between paratope and epitope residues. Based on a best constructed model of 6B4 bound with GPIbα, the survival ratios and rupture times of all detected hydrogen bonds and salt bridges in binding site were examined via free and steered MD simulations and regarded as indices of thermal and mechanical stabilizations of the bonds, respectively. Five principal paratope residues with their partners were predicted with their high survival ratios and/or long rupture times of involved hydrogen bonds, or with their hydrogen bond stabilization indices ranked in top 5. Exciting, the present results were in good agreement with previous mutagenesis experiment data, meaning a wide application prospect of our novel computational procedure on researches of molecular of basis of ligand-receptor interactions, various antithrombotic mAbs and other antibodies as well as theoretically design of biomolecular drugs.
Microparticle record in the Guliya ice core and its comparison with polar records since the last interglacial
Guangjian Wu,Tandong Yao,L. G. Thompson,Zhongqin Li
Chinese Science Bulletin , 2004, DOI: 10.1360/03wd0419
Abstract: Based on the study of oxygen isotope and microparticle in the Guliya ice core, atmospheric dust and environmental changes in the northwest Tibetan Plateau since the last interglacial were revealed. The microparticle record indicates that low dust load on the Plateau in the interglacial. Particle concentration increased rapidly when the climate turned into the last glacial and reached the maximum during the MIS 4. In the Last Glacial Maximum, however, the enhancement of microparticle concentration was slight, differing to those in the Antarctic and Greenland. On the orbital timescale, both the temperature on the Tibetan Plateau and summer solar insolation in the Northern Hemisphere had their impact on the microparticle record, but the difference in phase and amplitude also existed. Though having the same dust source, microparticle records in the ice cores on the Tibetan Plateau and the Greenland seem to have different significance.
Reusability in User Modeling
用户建模中的可重用性问题研究

Jiang Qi Li Guangjian,
江淇
,李广建

现代图书情报技术 , 2005,
Abstract: With the development of user information enviroment and user modeling, reusability emerges as a vital issue in user modeling applications. In this paper, we present two existing approaches to deal with the reusability issue, suggest a new hybrid approach, and discuss some possible problems in the new hybrid approach.
Design and Implement of Full-Text Retrieval System Based on WWW
基于WWW的全文检索系统设计与实现

Li Guangjian Huang Yongwen,
李广建
,黄永文

现代图书情报技术 , 2000,
Abstract: After discussing the definition, characteristics, development and current condition of the full-text retrieval system, the article introduces the general situation of “Educational Information Full-Text RetrievaI System Based on WWW”, and then analyzes the key components with which the system is implemented.
Iron content and solubility in dust from high-alpine snow along a north-south transect of High Asia
Guangjian Wu,Chenglong Zhang,Zhongqin Li,Xuelei Zhang
Tellus B , 2012, DOI: 10.3402/tellusb.v64i0.17735
Abstract: This study describes the dissolved and insoluble iron fraction of dust (mineral aerosol) in high-alpine snow samples collected along a north-south transect across High Asia (Eastern Tien Shan, Qilian Shan, and Southern Tibetan Plateau). This dust provides the basic chemical properties of mid- and high-level tropospheric Asian dust that can supply the limiting iron nutrient for phytoplankton growth in the North Pacific. The iron content in Asian dust averages 4.95% in Eastern Tien Shan, 3.38–5.41% along Qilian Shan and 3.85% in the Southern Tibetan Plateau. The iron fractional solubility averages about 0.25% in Eastern Tien Shan, 0.05–2% along Qilian Shan and 1.5% in the Southern Tibetan Plateau. Among the controlling factors that can affect iron solubility in Asian dust, such as dust composition and particle grain size, acidity seems to be the most significant and can increase the iron solubility by one or two orders of magnitude with acidification of pH=0.66. Our results reveal that iron solubility of dust in the remote downwind sites is higher than that in high-alpine snow, confirming the strong pH-dependence of iron solubility, and indicating that Asian dust shows a large variation in iron solubility on a regional scale.
In Vitro Toxicity of Naturally Occurring Silica Nanoparticles in C1 Coal in Bronchial Epithelial Cells
Guangjian LI,Yunchao HUANG,Yongjun LIU,Lv GUO
Chinese Journal of Lung Cancer , 2012, DOI: 10.3779/j.issn.1009-3419.2012.10.01
Abstract: Background and objective China’s Xuan Wei County in Yunnan Province have the world’s highest incidence of lung cancer in nonsmoking women-20 times higher than the rest of China. Previous studies showed, this high lung cancer incidence may be associated with the silica particles embedded in the production combustion from the C1 coal. The aim of this study is to separate the silica particles from production combustion from the C1 bituminous coal in Xuan Wei County of Yunnan Province, and study in vitro toxicity of naturally occurring silica particles on BEAS-2B. Methods ①Separating the silica particles from combustion products of C1 bituminous coal by physical method, observing the morphology by Scanning Electron Microscope, analysis elements by SEM-EDX, observed the single particle morphology by Transmission Electron Microscope, analyed its particle size distribution by Laser particle size analyzer, the surface area of silica particles were determined by BET nitrogen adsorption analysis; ②Cell viability of the experimental group (silica; naturally occurring), control group (silica; industrial produced and crystalline silica) was detected by assay used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and the reactive oxygen species (ROS), lactate dehydrogenase (LDH) were determined after 24 h-72 h exposed to these particles. Results ①The physical method can separate silica particles from production combustion from the C1 bituminous coal, which have different size, and from 30 nm to 120 nm particles accounted for 86.8%, different morphology, irregular surface area and containing trace of aluminum, calcium and iron and other elements; ②Under the same concentration, the experiment group have higher toxicity on BEAS-2B than control groups. Conclusion ①Physical method can separate silica particles from production combustion from the C1 bituminous coal and not change the original morphology and containing trace; ②Naturally occurring silica nanoparticles have irregular morphology, surface area, and containing complex trace elements may has greater toxicity than the silica nanoparticle of industrial produced and crystalline silica.
Chernobyl nuclear accident revealed from the 7010 m Muztagata ice core record
TIAN LiDe,YAO TanDong,WU GuangJian,LI Zhen,XU BaiQing,LI YueFang,
TIAN
,LiDe,YAO,TanDong,WU,GuangJian,LI,Zhen,XU,BaiQing,LI,YueFang

科学通报(英文版) , 2007,
Abstract: The total activity variation with depth from a 41.6 m Muztagata ice core drilled at 7010 m, recorded not only the 1963 radioactive layer due to the thermonuclear test, but also clearly the radioactive peak released by the Chernobyl accident in 1986. This finding indicates that the Chernobyl nuclear accident was clearly recorded in alpine glaciers in the Pamirs of west China, and the layer can be potentially used for ice core dating in other high alpine glaciers in the surrounding regions.
Recent high-resolution glaciochemical record from a Dasuopu firn core of middle Himalayas
WANG PengLing,YAO TanDong,TIAN LiDe,WU GuangJian,LI Zhen,YANG Wei,
WANG
,PengLing,YAO,TanDong,TIAN,LiDe,WU,GuangJian,LI,Zhen,YANG,Wei

科学通报(英文版) , 2008,
Abstract: A 16.8 m firn core of middle Himalayas was recovered on the col of Dasuopu glacier in August 2006, being 7000 m above sea level. A total of 317 samples were measured for stable oxygen isotope ratios (δ 18O) and major ion concentrations (Na+, NH 4 + , K+, Mg2+, Ca2+, Cl , SO 4 2 , and NO 3 ). The firn core dating and seasonal partitioning were carried out based on the marked seasonal variations along the stable oxygen isotopes and crustal species (Ca2+, Mg2+) profiles. The multi-parameters and high-resolution glaciochemical data set of Dasuopu firn core recorded the detailed chemical characteristics of precipitation in high-elevation region, middle Himalayas, since 1991 A.D., which mainly originated from the crustal and anthropogenic sources, while the sea-salt contribution was minor. The seasonal variability of major ion concentrations was dominated by the seasonal alternation of the prevalent air mass, atmospheric circulation situation and precipitation regime. Linear regression analysis indicated that most of the variance in annual ionic fluxes can be explained by a linear dependence on snow accumulation rate. Supported by the National Basic Research Program of China (Grant No. 2005CB422004), and the National Natural Science Foundation of China (Grant Nos. 40571039 and 40121101)
Chernobyl nuclear accident revealed from the 7010 m Muztagata ice core record
LiDe Tian,TanDong Yao,GuangJian Wu,Zhen Li,BaiQing Xu,YueFang Li
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0188-y
Abstract: The total activity variation with depth from a 41.6 m Muztagata ice core drilled at 7010 m, recorded not only the 1963 radioactive layer due to the thermonuclear test, but also clearly the radioactive peak released by the Chernobyl accident in 1986. This finding indicates that the Chernobyl nuclear accident was clearly recorded in alpine glaciers in the Pamirs of west China, and the layer can be potentially used for ice core dating in other high alpine glaciers in the surrounding regions.
Page 1 /127093
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.