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Search Results: 1 - 10 of 208771 matches for " Gracinda L.;LEONARDI "
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Estudo da libera??o renal de glicose em coelhos submetidos a hepatectomia total funcional e infus?o de noradrenalina
FRANCHI-TEIXEIRA, Antonio Roberto;JORGE, Gracinda L.;LEONARDI, Luís Sérgio;SAAD, Mário José Abdalla;
Arquivos de Gastroenterologia , 2001, DOI: 10.1590/S0004-28032001000300008
Abstract: aim ? to study the possible endogenous sources of glucose in the absence of the liver (equivalent to the anhepatic period of liver transplantation). material and methods - a experimental model of total functional hepatectomy in anesthetised rabbits was developed. the aorta and the right renal vein were catheterised in order to collect blood samples to measure glucose contents. the animals were divided into two groups: group 1, 5 animals underwent only norepinephrine infusion; group 2, 15 animals underwent norepinephrine infusion and submitted to total functional hepatectomy. results - in group 2, before the hepatectomy, arterial glucose levels were higher than venous ones and after the liver removal, the venous levels became higher than the arterial ones. this pattern showed an inversion in the glicemic curves. in group 1 this pattern was not observed. conclusion - the glicemic curves behavior observed in group 2 is not due to norepinephrine infusion, but represents renal glucose release after total functional hepatectomy.
Estudo da libera o renal de glicose em coelhos submetidos a hepatectomia total funcional e infus o de noradrenalina
FRANCHI-TEIXEIRA Antonio Roberto,JORGE Gracinda L.,LEONARDI Luís Sérgio,SAAD Mário José Abdalla
Arquivos de Gastroenterologia , 2001,
Abstract: Objetivo - Estudar as prováveis fontes endógenas de glicose na ausência do fígado (equivalente à fase anepática do transplante de fígado). Material e Métodos - Criou-se um modelo experimental de hepatectomia total funcional em coelhos anestesiados. A aorta e a veia renal direita foram cateterizadas para colheita de glicemias seriadas. Os animais foram divididos em dois grupos: grupo 1, com 5 animais submetidos apenas a infus o de noradrenalina e grupo 2, constando de 15 animais também submetidos a infus o de noradrenalina e tiveram seus fígados retirados. Resultados - Demonstram que, no grupo 2, antes da hepatectomia, as dosagens de glicemias arteriais foram maiores que as venosas, e após a retirada do órg o, estes valores se tornaram menores. Isto configurou invers o das curvas das glicemias. No grupo 1 n o foi observada essa invers o. Conclus o - Conclui-se que esse comportamento das curvas de glicemia observadas nos animais do grupo 2 n o se deve à a o da noradrenalina e significa libera o renal de glicose após a hepatectomia.
A new method for the experimental induction of secundary biliary cirrhosis in wistar rats
Jorge Gracinda De Lourdes,Leonardi Luiz Sergio,Boin Ilka de Fatima Santana Ferreira,Silva Jr Orlando de Castro e
Acta Cirurgica Brasileira , 2001,
Abstract: The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.
A new method for the experimental induction of secundary biliary cirrhosis in wistar rats
Jorge, Gracinda De Lourdes;Leonardi, Luiz Sergio;Boin, Ilka de Fatima Santana Ferreira;Silva Jr, Orlando de Castro e;Escanhoela, Cecilia Amelia Fazzio;
Acta Cirurgica Brasileira , 2001, DOI: 10.1590/S0102-86502001000200003
Abstract: the aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (sbf). forty-seven wistar rats were submitted to hepatic duct obstruction (ob group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. this technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. blood samples were collected for the measurement of total bilirubin (tb), alkaline phosphatase (ap), alanine aminotransferase (alt) and aspartate aminotransferase (ast). liver fragments were removed for morphological study. thirty days after surgery tb, ap, alt and ast levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of sbf in the ob group was confirmed by morphological study of the liver. there was technical failure in 31.92% cases. the survival was 100% at fifteen days and 82.97% at the end of the experiment. we concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. this technique can be carried out from cholestasis to fibrosis.
The airway epithelium dysfunction in the pathogenesis of asthma: The evidence  [PDF]
Salvatore Leonardi, Alfina Coco, Michele Miraglia Del Giudice, Gianluigi L. Marseglia, Mario La Rosa
Health (Health) , 2013, DOI: 10.4236/health.2013.52A044
Abstract:
Asthma is traditionally defined as a chronic, multisystem, multicellular disease characterized by bronchial hyper-responsiveness (BHR) and lung inflammation. In this illness is particularly involving the airway epithelium as the place where the inflammation begins, develops and often returns through airway remodelling. In specific way there is also an evolving awareness of the active participation of structural elements, such as the airway epithelium, airway smooth muscle, and endothelium, in this process. The airway inflammation and remodelling together likely explain the clinical manifestations of asthma that is demonstrated by complicated respiratory manifestations in which wheeze occurring secondary to bronchoconstriction in the setting of airway hyper-responsiveness and mucous hypersecretion. This is confirmed by also the histopathological analyses of bronchial biopsies from asthmatic subjects. For this reason is very important to understand the physiological role of the airway epithelium and their changes during the exacerbation of disease.
PROM:A Support for Robust Replication in a Distributed Object Environment
A.Corradi,L.Leonardi
计算机科学技术学报 , 1990,
Abstract: The concept of object can be employed to achieve tolerance to hardware faults in distributed systems.Replication by introducing several copies for each object allows a continuous service even in case of failure.In particular,the paper describes an object model,PROM,which exploits replication by defining several passive back-up copies for any object.The system automatically reovers any failure of a copy in execution by activating a spare copy and restarting it from a previous checkpoint.The aim of the paper is the analysis of the effective support for PROM.This support is organized in structured levels on a distributed architecture.The services that the support should include to guarantee the desired replication model are described.
PMWS (Postweaning Multisystemic Wasting Syndrome) In Central Italy: Histopathological, Immunohistochemical and Ultrastructural Findings
L. Leonardi, L. Mechelli, E. Lepri, E. Manuali* and G. Vitellozzi
International Journal of Veterinary Science , 2012,
Abstract: We describe the presence of Porcine circovirus 2 (PCV-2) in thirteen pigs necropsied in Department of Biopathological Sciences and Hygiene of Animal and Alimentary Productions in Perugia. Sistemic lymphadenopathy, sometimes haemorrhagic and necrotic, spleen enlarged thymus atrophic, lungs collapse and consolidated, were the principal gross findings. The most consistent histological lesion was a moderate to severe lymphocellular depletion in lymphoid organs, syncytial giant cell formation, and intracytoplasmic inclusion bodies in macrophages. PCV-2 was immunohistochemically detected in all pigs with PMWS. Electron microscopy investigations demonstrated viral particles compatible with Porcine PCV-2.
Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies
Silva-Froufe, Lúcia Gracinda da;Boddey, Robert Michael;Reis, Veronica Massena;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000400018
Abstract: the species gluconacetobacterdiazotrophicus, herbaspirillum seropedicae and h. rubrisubalbicans are endophytic n2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. however, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in n free semi-solid media which are only semi-selective for the species/genera [the most probable number (mpn) technique] and each culture must be further subjected to several tests to identify the isolates at the species level. the use of species-specific polyclonal antibodies with the indirect elisa (enzyme-linked immunosorbent assay) can be an alternative which is rapid and specific to quantify these populations of bacteria. this study was performed to investigate the viability of adapting the indirect elisa technique to quantify individually the populations of these three species of diazotroph within the root and shoot tissues of sugarcane. the results showed that species-specific polyclonal antibodies could be obtained by purifying sera in protein-a columns which removed non-specific immuno-globulins. it was possible to quantify the three bacterial species in the brazilian sugarcane variety sp 70-1143 in numbers above 105 cells per g fresh weight in roots, rhizomes and leaves. the numbers of the different bacterial species evaluated using the elisa technique were found to be higher than when the same populations were evaluated using the mpn technique, reaching 1400 times greater for g. diazotrophicus and 225 times greater for herbaspirillum spp. these results constitute the first quantification of herbaspirillum using immunological techniques.
Herbaspirillum seropedicae and sugarcane endophytic interaction investigated by using high pressure freezing electron microscopy
Silva, Lúcia Gracinda da;Miguens, Flávio Costa;Olivares, Fabio Lopes;
Brazilian Journal of Microbiology , 2003, DOI: 10.1590/S1517-83822003000500023
Abstract: the interaction between sugar cane plantlets and h. seropedicae was investigated using high pressure freezing followed by freeze substitution. microscopical observation showed consistent differences between this approaches when compared with the conventional preparation, specially related to appearance of the bacteria cell and the endophytic attachment to the host cell wall.
Promo??o de enraizamento de microtoletes de cana-de-a?úcar pelo uso conjunto de substancias húmicas e bactérias diazotróficas endofíticas
Marques Júnior, Roberto Batista;Canellas, Luciano Pasqualoto;Silva, Lúcia Gracinda da;Olivares, Fábio Lopes;
Revista Brasileira de Ciência do Solo , 2008, DOI: 10.1590/S0100-06832008000300020
Abstract: besides the direct nutritional effect of mineralization of organic matter or by biological nitrogen fixation activity, the humic substances and endophytic diazotrophic bacteria can directly influence plant metabolism, modifying plant growth and development patterns. the purpose of this study was to evaluate the effect of the combined use of humic acid (ha) and herbaspirillum seropedicae, an endophytic nitrogen-fixing bacteria, on the root growth of seed pieces - heat-treated or not - of the sugarcane variety rb 72 454. after hot water treatment, the cane cuttings were immersed for 12 hours as follows: in water (control plant), in ha solution (20 mg l-1 of c) from vermicompost, in bacterial inoculant of h. seropedicae, strain hrc54 (108 cells ml-1), and in a combination of bacteria and ha. root growth was improved by 60 to 118 % in length and from 33 % to 233 % in surface area on sugarcane plant treatments compared to control, with more pronounced effect in plants under heat treatment. likewise, the positive effect of the combinded treatment (bacteria inoculation and humic acid) on shoot and root biomass was significant compared to the control with heat-treated cuts. for non-heated seed pieces, bacteria inoculation did not result in a positive plant growth effect, but only in the presence of humic acid. the combined or isolated use of both ha and bacteria did not significantly modify the bacteria population in the root tissue of heat-untreated sugarcane. for heat-treated cuts, bacteria inoculation, combined or not with ha, increased the size of diazotrophic bacteria population on roots. the results highlight the importance of thermotherapy to increase the positive effect of the selected bacteria strain of h. seropedicae. in the case of ha application, plant growth stimulation was not related to thermotherapy. the plant root growth promoting effect induced by both ha and the inoculation of selected nitrogen-fixing bacteria strain could represent an innovative approach
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