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Search Results: 1 - 10 of 28323 matches for " Gopal Kumar Sharma "
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Development and Evaluation of Shelf Stability of Flaxoat Nutty Bar in Different Packaging Materials  [PDF]
Ananthan Padmashree, Gopal Kumar Sharma, Thimma Govindaraj
Food and Nutrition Sciences (FNS) , 2013, DOI: 10.4236/fns.2013.45069

Flaxoat nutty bar, a granola cereal bar was developed using oat and wheat as major cereal ingredients and their shelf life was assessed in polypropylene (PP 75 μ), paper (42 GSM) aluminium foil (20 μ) polyethylene (PFP, 37.5 μ) and metallised polyester (MP, 12 μ) low density/linear low density (150 μ) (with and without vacuum) films and stored under ambient temperature conditions and 37. Chemical changes in bar under different packaging materials during storage revealed that, the bar remained stable and acceptable for 12 months at ambient conditions irrespective of the packaging materials used, while at 37, the bar packed in PP films remained stable for 6 months only and rest of the bars packed in PFP, MP and MP (vacuum) films found stable and acceptable for the entire storage period of 12 months. The effect of aw on lipid peroxidation established the maximum stability of the bar at 0.33 aw. Fourteen fatty acids were identified in which oleic (50.91%), linoleic (22.50%) and palmitic (12.63%) acids were found to be the major fatty acids. Fortification of a bar with vitamin C, B1 and B2 contents to meet 50% RDA enhanced vitamin contents and the maximum retention was observed in bars packed under vacuum in MP films.

Antioxygenic Activity of Solanum nigrum L. Leaves in Sunflower Oil Model System and Its Thermal Stability  [PDF]
Ananthan Padmashree, Gopal Kumar Sharma, Anil Dutt Semwal, Chitrashekarachar Mahesh
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.511113
Abstract: The antioxygenic activity of Solanum nigrum L. leaves and its various solvent extracts were evaluated using sunflower oil model system. Leaf powder and its methanol/water (80:20) soluble fraction showed strong antioxygenic activity in refined sunflower oil. On the other hand, ethyl acetate fraction exhibited marginal antioxygenic activity, whereas the water soluble fraction was practically devoid of any activity in refined sunflower oil. Thermal stability of different extracts of Solanum nigrum L. leaves heated at 80?C in refined sunflower oil also indicated the strong efficacy of methanol/water (80:20) extract to inhibit thermal oxidation. Solanum nigrum L. contain high levels of magnesium (239.0 mg/100g) and phosphorous (80.3 mg/100g). Fatty acid analysis of the lipid extracted from Solanum nigrum L. leaves indicated the presence of linoleic (59.1%) as a major fatty acid. The result of this study confirmed the presence of antioxygenic compounds in leaves, in particular its methanol/water (80:20) extracts showed great potential as a natural antioxidant to inhibit lipid peroxidation in foods.
Optimization of Multigrain Premix for High Protein and Dietary Fibre Biscuits Using Response Surface Methodology (RSM)  [PDF]
Kathalsar Ashwath Kumar, Gopal Kumar Sharma, Mohammed Ayub Khan, Anil Dutt Semwal
Food and Nutrition Sciences (FNS) , 2015, DOI: 10.4236/fns.2015.69077
Abstract: In order to improve the nutritional quality of biscuits, a multigrain premix (MGP) was developed by using whole barley, sorghum, chickpea, pea and defatted soya flour, each at 20% level. The developed MGP had 26.28% protein, 10.13% insoluble dietary fiber and 7.38% soluble dietary fiber. The experiment was designed to optimise the MGP and wheat flour concentration for the development of multigrain biscuits with high protein, dietary fibre and to maximize the acceptability by the application of central composite rotatable design (CCRD) of Response Surface Methodology (RSM). The levels of incorporation of MGP and wheat flour were taken as variables whereas protein, soluble, insoluble fibers, biscuit dough hardness, breaking strength and overall acceptability (OAA) as responses. The optimum level of MGP and wheat flour obtained using numerical optimization was found to be 40 g and 60 g respectively. The biscuits prepared using these had 16.61% protein, 2.57% soluble fibre, and 6.67% insoluble fibre which is significantly (p ≤ 0.05) higher than control biscuit.
Utilization of Virgin Coconut Meal (VCM) in the Production of Ready-to-Eat Indian Traditional Sweet Meat Using Response Surface Methodology  [PDF]
Yashi Srivastava, Anil Dutt Semwal, Gopal Kumar Sharma, Amarinder Singh Bawa
Food and Nutrition Sciences (FNS) , 2011, DOI: 10.4236/fns.2011.23029
Abstract: Virgin coconut meal (VCM) was used for the development of ready-to-eat Indian traditional sweet meat commonly known as “ladoo” both with and without sorbic acid (0.3%). The different ingredients were optimized following re-sponse surface methodology with three independent variables i.e. sugar, water and VCM and sensory attributes as responses. The changes in the quality of VCM ladoo packed in polypropylene (PP, 75 µ) and laminates of metalized polyester (MP, 75 µ) were monitored during storage to establish the shelf life under ambient temperature conditions (15?C - 34?C). Samples without added potassium sorbate spoiled within three weeks of storage due to mold growth and fermented odour. VCM ladoo containing sorbic acid did not support any microbial growth during storage up to 4 months, however, during storage samples packed in PP lost moisture and became hard and brittle, while those packed in MP remained soft. Peroxide and thiobarbituric acid values were higher in PP packed samples as compared to those packed in MP. Sorbic acid degraded during storage of ladoo and the rate of degradation was higher in PP packed ladoos than that in MP packed ones.
Studies on the Optimization and Storage Stability of Virgin Coconut Meal Incorporated Instant Sooji Halwa Mix  [PDF]
Mohammed Ayub Khan, Mahesh Chitrashekarchar, Anil Dutt Semwal, Gopal Kumar Sharma
Food and Nutrition Sciences (FNS) , 2012, DOI: 10.4236/fns.2012.38145
Abstract: Virgin Coconut Meal (VCM) was used for the development of instant wheat sooji (semolina) halwa mix with better nutritional attributes. Central composite rotatable design (CCRD) with 2 independent variables (sugar and VCM) and 4 responses (lightness, redness, taste and overall acceptability) was used for the optimisation. VCM incorporated instant halwa mix prepared using optimised levels of ingredients contained moisture 0.95%; fat 26.2%; protein 7.65%; total ash 0.86%; fibre 1.02% and received overall acceptability score of 8.5 on a 9 point hedonic scale providing 523.86 K·cal/ 100g. The changes in quality of stored VCM incorporated instant halwa mix packed in polypropylene (PP, 75 μ) and laminates of metallised polyester (MP, 90 μ) were monitored in order to assess the shelf-life. Instant halwa mix remained stable and acceptable for one year in both the packaging materials under ambient temperature conditions (15℃ - 34℃). However, the rate of lipid peroxidation was found to be slightly higher in PP packed samples as compared to MP packed ones. Fatty acid composition of VCM incorporated instant halwa mix remained practically unchanged during storage. Oleic acid was the major fatty acid present in fat extracted from halwa mix followed by palmitic and lauric acids.
In Vitr Antioxygenic Activity of Ridge Gourd (Luffa acutangula) Pulp, Peel and Their Extracts on Peroxidation Models  [PDF]
Ananthan Padmashree, Gopal Kumar Sharma, Anil Dutt Semwal, Amarinder Singh Bawa
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.310171
Abstract: Ridge gourd pulp and peel powders as well as their various solvent fractions were evaluated for antioxygenic activity using different methods. Ridge gourd pulp and peel powders at 2% level and their ethanol/water soluble extracts exhibited strong antioxygenic activity in sunflower oil stored at 37?C. The water soluble extract devoid of any antioxygenic activity in sunflower oil. Ridge gourd pulp and peel powders as well as their extracts were evaluated for their antioxygenic activity using linoleic acid peroxidation, β-carotene-linoleic acid bleaching and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) methods. Ethanol/water extracts from ridge gourd pulp and peel showed highest antioxygenic activity followed by water extracts, while the petroleum ether extract showed moderate antioxygenic activity. Ridge gourd peel powder and its extracts showed slightly higher antioxygenic activity than ridge gourd pulp powder and its extracts. This may be attributed to the presence of higher amounts of phenolics and flavonoids which have been reported as potential antioxidants.
Transcriptional regulation of human osteopontin promoter by histone deacetylase inhibitor, trichostatin A in cervical cancer cells
Priyanka Sharma, Santosh Kumar, Gopal C Kundu
Molecular Cancer , 2010, DOI: 10.1186/1476-4598-9-178
Abstract: In this paper, we for the first time report that TSA suppresses PMA-induced OPN gene expression in human cervical carcinoma cells and previously unidentified AP-1 transcription factor is involved in this event. Deletion and mutagenesis analyses of OPN promoter led to the characterization of a proximal sequence (-127 to -70) that contain AP-1 binding site. This was further confirmed by gel shift and chromatin immunoprecipitation (ChIP) assays. Western blot and reverse transcription-PCR analyses revealed that TSA suppresses c-jun recruitment to the OPN promoter by inhibiting c-jun levels while c-fos expression was unaffected. Silencing HDAC1 followed by stimulation with PMA resulted in significant decrease in OPN promoter activity suggesting that HDAC1 but not HDAC3 or HDAC4 was required for AP-1-mediated OPN transcription. TSA reduces the PMA-induced hyperacetylation of histones H3 and H4 and recruitment of RNA pol II and TFIIB, components of preinitiation complex to the OPN promoter. The PMA-induced expression of other AP-1 regulated genes like cyclin D1 and uPA was also altered by TSA. Interestingly, PMA promoted cervical tumor growth in mice xenograft model was significantly suppressed by TSA.In conclusion, these findings provide new insights into mechanisms underlying anticancer activity of TSA and blocking OPN expression at transcriptional level by TSA may act as novel therapeutic strategy for the management of cervical cancer.Osteopontin (OPN) is a secreted, noncollagenous, sialic acid-rich, cytokine-like glycosylated phosphoprotein which is a member of Small Integrin Binding LIgand N-linked Glycoprotein (SIBLING) family and plays important role in determining the oncogenic potential of various cancers [1,2]. OPN expression is increased in a variety of cancers and is reported to correlate with enhanced tumor progression and metastasis [3-5]. OPN plays crucial roles in cancer cell metastasis, granuloma formation, dystrophic calcification and in coronary restenos
Production of moderately halotolerant, SDS stable alkaline protease from Bacillus cereus MTCC 6840 isolated from lake Nainital, Uttaranchal state, India
Joshi, Gopal K.;Kumar, Sarvesh;Sharma, Vinay;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000400034
Abstract: a moderately cold active, extracellular alkaline protease producing bacterium was isolated from a fresh water lake. the isolate was found to be a gram-positive, rod shaped organism later identified as bacillus cereus mtcc 6840. the bacterium produced the maximum amount of enzyme when allowed to grow for 24 h at temperature 25o and ph 9.0. among a variety of substrates used, fructose as a carbon source and a combination of yeast extract and peptone as nitrogen source, supported the maximum protease production by the organism (120 u/ml). fe++ and co++ stimulated the enzyme activity whereas ca++, cu++, k+, mg++ and mn++ inhibited it to different extents. the protease was found to be highly stable in the presence of nacl, sds and acetone. treatment with edta and pmsf resulted in the considerable loss of enzyme activity. the enzyme was found to be optimally active at ph 9.0 and temperature 20oc.
Development and Quality Evaluation of Choco Quinoa Nutri Bar during Storage  [PDF]
Ananthan Padmashree, Neha Negi, Soorya Haridas, Thimma Govindaraj, Kandanganth Raghavan Anila Kumar, Anil Dutt Semwal, Gopal Kumar Sharma
Food and Nutrition Sciences (FNS) , 2018, DOI: 10.4236/fns.2018.97067
Abstract: Choco quinoa nutri bar was developed using quinoa as one of the ingredients to explore its nutritional benefits. Bars were packed in polypropylene (PP, 75 μ) and metallised polyester (12 μ) low density—high density (MP, 100 μ) (with/without vacuum) films, stored under ambient and 37°C temperature conditions for shelf life evaluation. Bar contained 14.43% protein, 14.93% fat with a calorific value of 426.75 k cal/100g. Chemical changes were observed significantly (p < 0.05) more in the samples stored at 37°C than the ones stored at ambient conditions (15°C - 34°C). Bars showed maximum stability at 0.33 aw with less chemical changes. Oleic acid (36.06%) was found to be the major fatty acids in the bar followed by palmitic (29.35%), stearic (17.12%) and linoleic (12.05%) acids. Hardness of the bar enhanced significantly during storage, and was observed significantly (p < 0.05) higher in the samples packed in MP films (with/without vacuum) as compared to bars packed in PP films thus restricting the shelf life of the bar to 6 months in MP films (with/without vacuum) and 9 months in PP films at both the temperature conditions.
Contamination of Lead and Mercury in Coal Basin of India  [PDF]
Reetu Sharma, Shobhana Ramteke, Khageshwar Singh Patel, Sudhanshu Kumar, Bighnaraj Sarangi, Shankar Gopal Agrawal, Lesia Lata, Huber Milosh
Journal of Environmental Protection (JEP) , 2015, DOI: 10.4236/jep.2015.612124
Abstract: The coal is contaminated with toxic metals at the trace levels. They are released into the environment during mining, handling and burning of coal. The Korba basin has one of the largest coal exploitation areas in the country. In this work, contamination and sources of toxic metals i.e. Hg and Pb in the air, soil and sediment of the Korba basin, India are described. The concentration of Hg and Pb in the ambient air was ranged from 7.4 - 29 and 7.0 - 585 ng/m3 with mean value of 18 ± 4 and 129 ± 104 ng/m3 in the winter season. The mean concentration of Hg in the soil and sediment was 0.22 ± 0.03 and 0.44 ± 0.08 mg/kg, respectively. The higher concentration of Pb in the environmental samples was observed.
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