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Search Results: 1 - 10 of 39761 matches for " Glutathione-S-Transferases "
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人类常用止痛剂布洛芬(Ibuprofen)及乙酰胺酚(Acetaminophen)对多齿新米虾(Neocaridina denticulate)的影响
Effect of Drug Residues of Ibuprofen and Acetaminophen on Aquatic Neocaridina denticulate
 [PDF]

赖慧绮, 蔡宜君, 叶芳伶, 宋宏红, 陈健民, 黄大骏
International Journal of Ecology (IJE) , 2012, DOI: 10.12677/ije.2012.12004
Abstract:

近年来许多研究指出,人类长期使用的药物如抗生素或止痛剂等医疗药品,无法经由污水处理厂而完全分解消失,当这些药物进入环境中时将可能具有生物毒性,并对生态环境系统造成冲击。为了解人类常用止痛剂(ibuprofen, IBU)及乙酰胺酚(acetaminophen, ACE)残留于水体后对水生生物可能的影响。本研究应用多齿新米虾(Neocaridina denticulate,后简称米虾)进行0.115 mg/LIBUACE不同浓度的曝露。并于曝露后147天,并测定米虾体内解毒酵素的活性(monooxygenase, Mon)glutathione-S-transferase, GST)与肝胰脏受损指标(glutamic oxaloacetic transaminase, GOT)(glutamic pyruvic transaminase, GPT)。试验结果显示,米虾曝露IBUACE147天,各处理组间Mon
Modeling analysis of GST (glutathione-S-transferases) from Wuchereria bancrofti and Brugia malayi
Rayavarapu Bhargavi,Siddharth Vishwakarma,Upadhyayula Suryanarayana Murty
Bioinformation , 2005,
Abstract: GST (glutathione S-transferases) are a family of detoxification enzymes that catalyze the conjugation of reduced GSH (glutathione) to xenobiotic (endogenous electrophilic) compounds. GST from Wb (Wuchereria bancrofti) and Bm (Brugia malayi) are significantly different from human GST in sequence and structure. Thus, Wb-GST and Bm-GST are potential chemotherapeutic targets for anti-filarial treatment. Comparison of modeled Wb and Bm GST with human GST show structural difference between them. Analysis of the active site residues for the binding of electrophilic co-substrates provides insight towards the design of parasite specific GST inhibitors.
Evaluation of Glutathione-S-transferase and ceruloplasmin levels in gingival crevicular fluid and gingival tissue as diagnostic markers for chronic periodontitis  [PDF]
Jothi. M. Varghese, Vinutha Bhat, G. S. Bhat, Namita Rao
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.324061
Abstract: Periodontitis, is an infectious ailment of multifactorial origin, that brings about destruction of bone and surrounding tissues. There are various oral pathogens that may be responsible for the destruction. The host encounters these microbial invasions and their products by the production and release of inflammatory mediators from the cells within the body. Glutathione-S-transferase (GST) are a group of enzymes that utilize glutathione in conditions resulting in oxidative stress. These enzymes play a key role in the detoxifycation of such substance. It aids in preventing damage to important cellular components caused by release of free reactive oxygen species. Ceruloplasmin is a ferroxidase enzyme. It plays a role as an anti-inflammatory agent, by its ability to scavenge free radicals within the body. The present study was targeted at evaluating the levels of Glutathione-S-Transferase (GST) and Ceruloplasmin as diagnostic markers for patients with chronic periodontitis in gingival crevicular fluid (GCF) and the gingival tissues. Thirty patients were divided into two groups. Experimental group comprising of 15 subjects with chronic perio- dontitis and the control group was composed of 15 healthy individuals. Highly significant changes in GST between the diseased and normal patients (P = 0.001) were detected. There was a decrease in GST level in both gingival tissue & GCF in diseased patients when compared to the control patients. The ceruloplasmin levels in GCF and gingival tissues showed no difference between the control and diseased group. Hence,these results indicate a relationship suggesting that GST produced during chronic inflammation could be used as biomarker that indicate periodontal disease .
Plasma catalase, glutathione-s-transferase and total antioxidant activity levels of children with attention deficit and hyperactivity disorder  [PDF]
V. Kenan ?elik, Erdal Er?an, Serpil Er?an, Sevtap Bak?r, Orhan Dogan
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.42026
Abstract:
Objective: In this study, we plan to measure plasma Catalase (CAT), Antioxidant Activity (AOA) and Glu- tathione-S-Transferase (GST) levels to understand whether oxidative stress develops or not and whether or not the detoxification mechanism properly functions in children with Attention Deficit and Hyperactivity Disorder (ADHD) with unknown etiology and pathogenesis. Method and Results: Plasma CAT, AOA, and GST activities were spectrophotometrically measured in forty patients (average age 10.27 ± 2.54) and thirty-five (average age, 9.97 ± 2.59) healthy individuals as the control group. While the CAT activity showed no difference in the patient group (P > 0.05) compared to the control group, AOA and GST levels were found significantly meaningful (P = 0.001). Conclusion: In this pilot study ,the study shows that no oxidative stress develops in individuals with ADHD in high AOA and stable CAT activity, and that the de- toxification mechanism functions extremely in high GST activity. These findings need to be supported by other studies.
Increased serum homocysteine levels and glutathione-S-transferase activities in alcoholic patients attending de-addiction centre
Kulkarni S.R.,Ravindra К.Р.,Dhume С.,Rataboli P.V.
Saratov Journal of Medical Scientific Research , 2010,
Abstract: The purpose of this study was to investigate the effect of heavy alcohol consumption on serum Malondialdehyde, homocysteine status and glutathione-S -transferase (GST) activities in alcoholics consuming illicit liquor from lower socioeconomic background attending deaddiction centre. The study was conducted in ninety alcoholic patients consuming illicit liquor from lower socio-economic background attending de-addiction centre and compared to healthy non alcoholic controls (n=90). Serum Malondialdehyde (MDA), serum homocysteine and activities of antioxidant enzyme glutathione-S - transferase (GST) were estimated. Alcoholics consuming illicit liquor attending de-addiction centre displayed significantly higher values of serum MDA concentration, serum homocysteine levels and serum GST activities as compared to their non alcoholic healthy controls belonging to the same socio-economic background. Our results indicate that increase in serum Malondialdehyde (MDA) concentration marker of oxidative stress, serum homocysteine levels and serum glutathione-S-transferase (GST) activities may enhance the susceptibility to vascular diseases in heavy illicit drinkers with poor nutritional status
Prognostic Significance of Comparison of Clinical Indicators with Manifestations of Genetic Polymorphism of Glutathione-S-Transferases in Non-Small Cell Lung Cancer  [PDF]
Mikhail N. Shapetska, Evelina V. Krupnova, Alena P. Mikhalenka, Natalia V. Chebotareva, Anna N. Shchayuk, Svetlana G. Pashkevich, Alexander V. Prokhorov
Journal of Cancer Therapy (JCT) , 2018, DOI: 10.4236/jct.2018.912080
Abstract: The article presented the results of comparison of polymorphic variants of the genes GSTM1, GSTT1, GSTP1 and clinical manifestations of non-small cell lung carcinoma. The association of the genotype GSTT1 (del) with the risk of developing squamous cell lung cancer has been revealed (OR = 2.54 CI: 1.13 - 5.72, p = 0.035). Analysis of patient survival rate (n = 173) in groups of various histological types of lung cancer showed that in the group of squamous cell lung cancer (n = 91) in patients with genotype GSTT1 (del), the survival rate median was significantly higher—84 months (95% CI 12.4 - 155.7) than in patients with the genotype GSTT1 (+)—36 months (95% CI 25.2 - 46.8, p = 0.045). In contrast, in the adenocarcinoma group (n = 82), the survival rate median in patients with the genotype GSTT1 (del) was 19 months. (95% CI 6.2 - 33.5), and in patients with genotype GSTT1 (+)—67 months (95% CI 50.1 - 84.0), which is the basis for continuing this comparison in an additional group of testees, as the sampling did not achieve the reliability of p = 0.12. Hypothetically, these differences may be due to differences in the gender composition of squamous cell lung cancer and adenocarcinoma and the involvement of GST enzymes in the metabolism of estrogens in adenocarcinoma in women and other hormonal background and reactivity of the male body with squamous cell carcinoma. Further research and subsequent analysis of the results will be aimed at confirming this hypothesis.
Emerging clinical and therapeutic applications of Nigella sativa in gastroenterology
Shailendra Kapoor
World Journal of Gastroenterology , 2009,
Abstract: Nigella sativa (N. sativa) decreases DNA damage and thereby prevents initiation of carcinogenesis in colonic tissue secondary to exposure to toxic agents such as azoxymethane. N. sativa is of immense therapeutic benefit in diabetic individuals and those with glucose intolerance as it accentuates glucose-induced secretion of insulin besides having a negative impact on glucose absorption from the intestinal mucosa. N. sativa administration protects hepatic tissue from deleterious effects of toxic metals such as lead, and attenuates hepatic lipid peroxidation following exposure to chemicals such as carbon tetrachloride.
Serum total glutathione-s-transferase levels in oral cancer
Prabhu Krishnananda,Bhat Gopalakrishna
Journal of Cancer Research and Therapeutics , 2007,
Abstract: We conducted a study wherein serum total glutathione-s-transferase levels were measured in patients (n = 27) with various stages of biopsy proven oral cancer (squamous cell carcinoma) and age and sex matched healthy human volunteers (n=10). In all patients with oral cancer, serum total glutathione-s-transferase was measured before the onset of treatment. There was a significant increase in serum total glutathione-s-transferse levels in patients with stage IV oral cancer as compared to stage II (P = 0.001) and stage III (P = 0.002) oral cancer. This shows that alterations in serum total Glutathione-s-transferase levels may have a role in cancer progression.
Natural Products Modulate the Multifactorial Multidrug Resistance of Cancer  [PDF]
Safaa Yehia Eid, Mahmoud Zaki El-Readi, Sameer Hassan Fatani, Essam Eldin Mohamed Nour Eldin, Michael Wink
Pharmacology & Pharmacy (PP) , 2015, DOI: 10.4236/pp.2015.63017
Abstract: Multidrug resistance (MDR) is a critical problem in cancer chemotherapy. Cancer cells can develop resistance not only to a single cytotoxic drug, but also to entire classes of structurally and functionally unrelated compounds. Several mechanisms can mediate the development of MDR, including increased drug efflux from the cells by ABC-transporters (ABCT), activation of metabolic enzymes, and defective pathways towards apoptosis. Many plant secondary metabolites (SMs) can potentially increase sensitivity of drug-resistant cancer cells to chemotherapeutical agents. The present thesis investigates the modulation of MDR by certain medicinal plants and their active compounds. The inhibition of ABCTs (P-gp/MDR1, MRP1, BCRP) and metabolic enzymes (GST and CYP3A4), and the induction of apoptosis are useful indicators of the efficacy of a potential medicinal drug. The focus of this study was the possible mechanisms of drug resistance including: expression of resistance proteins, activation of metabolic enzymes, and alteration of the apoptosis and how to overcome their resistance effect on cancer cells. The overall goal of this review was to evaluate how commonly used medicinal plants and their main active secondary metabolites modulate multidrug resistance in cancer cells in order to validate their uses as anticancer drugs, introduce new therapeutic options for resistant cancer, and facilitate the development of their anticancer strategies and/or combination therapies. In conclusion, SMs from medicinal plants exhibit multitarget activity against MDR-related proteins, metabolic enzymes, and apoptotic signaling, this may help to overcome resistance towards chemotherapeutic drugs.
Changes in endogenous tissue glutathione level in relation to murine ascites tumor growth and the anticancer activity of cisplatin
Khynriam, D.;Prasad, S.B.;
Brazilian Journal of Medical and Biological Research , 2003, DOI: 10.1590/S0100-879X2003000100008
Abstract: changes in glutathione levels were determined in tissues of 11- to 12-week-old swiss albino mice at different stages of dalton's lymphoma tumor growth and following cisplatin (8 mg/kg body weight, ip) treatment for 24-96 h, keeping 4-5 animals in each experimental group. glutathione levels increased in spleen of tumor-bearing compared to normal mice (9.95 ± 0.14 vs 7.86 ± 1.64 μmol/g wet weight, p£0.05) but decreased in blood (0.64 ± 0.10 vs 0.85 ± 0.09 mg/ml) and testes (9.28 ± 0.15 vs 10.16 ± 0.28 μmol/g wet weight, p£0.05). dalton's lymphoma cells showed an increase in glutathione concentration (4.43 ± 0.26 μmol/g wet weight) as compared to splenocytes, their normal counterpart (3.62 ± 0.41 μmol/g wet weight). with the progression of tumor in mice, glutathione levels decreased significantly in testes (~10%) and bone marrow cells (~13%) while they increased in dalton's lymphoma cells (28-46%) and spleen (15-27%). glutathione levels in kidney, dalton's lymphoma cells and bone marrow cells (8.50 ± 1.22, 4.43 ± 0.26 and 3.28 ± 0.17 μmol/g wet weight, respectively) decreased significantly (6.04 ± 0.42, 3.51 ± 0.32 and 2.17 ± 0.14 μmol/g wet weight, p£0.05) after in vivo cisplatin treatment for 24 h. along with a decrease in glutathione level, the glutathione-s-transferase (gst) activity also decreased by 60% in tumor cells after cisplatin treatment. the elevated drug uptake by the tumor cells under the conditions of reduced glutathione concentration and gst activity after treatment could be an important contributory factor to cisplatin's anticancer activity leading to tumor regression. furthermore, lower doses of cisplatin in combination with buthionine sulfoximine (an inhibitor of glutathione synthesis) may be useful in cancer chemotherapy with decreased toxicity in the host.
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