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Search Results: 1 - 10 of 34313 matches for " Gisele Rodrigues;Ferreira "
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Compara??o de três protocolos distintos para extra??o de RNA de amostras fixadas em formalina e emblocadas em parafina
Gouveia, Gisele Rodrigues;Ferreira, Suzete Cleusa;Sabino, Ester Cerdeira;Siqueira, Sheila Aparecida Coelho;Pereira, Juliana;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2011, DOI: 10.1590/S1676-24442011000600012
Abstract: introduction: formalin fixed paraffin embedded (ffpe) tissues are an important sample source for retrospective studies. despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests once it fragments and chemically modifies nucleic acids, particularly rna. objective: to compare the efficiency of three different rna extraction protocols for gene expression analysis of ffep tissues. material and methods: rna was extracted from ffpe samples of human lymph by means of ambion and arcturus bioscience kits and the classical trizol method. after extraction, rna was quantified and tested for amplification through real time polymerase chain reaction (rt-pcr) using glyceraldehydes-3 phosphate dehydrogenase (gapdh) endogenous gene primers. discussion/conclusion: all the protocols produced sufficient and adequate amounts of total rna. however, only protocols using arcturus and ambion kits generated suitable rna for pcr amplification.
Avalia??o preliminar da escala de desempenho em informática educacional com professores
Joly, Maria Cristina Rodrigues Azevedo;Franco, Gisele de Sousa;Nicolau, Adriana Ferreira;
Estudos de Psicologia (Campinas) , 2004, DOI: 10.1590/S0103-166X2004000300007
Abstract: the information technology is an education elementary tool. an instrument able to analyze teachers performance using this technology as an educational tool is very important. this research purpose was to evaluate the psychometrics characteristics of the educational informatics performance scale through an online pilot study. 82 elementary teachers from public and private schools were the subjects of this study. educational informatics performance scale, which was answered online, was composed by 51 items with 4 answer choices each one in order to define every teacher's profile. the results has presented a good internal consistency (αcronbach =0,9678) and a 0,3792 average items correlation. the instrument internal precision has identified the items homogeneity, and it was verified by the split-half method. the factorial analysis has suggested some items rearranges according to the scale definitions. the psychometrics qualities added to the results of factorial analysis has revealed the educational informatics performance scale effectiveness validity.
Caracteriza??o física de frutos e putamens e taxa de ataque por Carmenta sp. a pequizeiros (Caryocar brasiliense Camb.) no norte de Minas Gerais
Luz, Giovana Rodrigues da;Rodrigues, Priscyla Maria Silva;Menino, Gisele Cristina de Oliveira;Coutinho, Etiene Silva;Nunes, Yule Roberta Ferreira;
Revista Brasileira de Fruticultura , 2011, DOI: 10.1590/S0100-29452011005000088
Abstract: the pequi tree is a native species of brazilian savanah, which fruits are explored in an extractivist form, having a huge economic importance. the long periods of availability of the fruits, together with the heterogeneity of the productive regions, leads to an inference of the existence of differences among their physical characteristics. thus, this study aimed to compare the physical characteristics of pequi tree fruit collected in three municipalities in the north of minas gerais state, and to verify if the attack by insects is directly related to it's biometrical characteristics. we sampled 10 individuals and collected 20 fruits from each tree, in montes claros, mirabela and japonvar, municipalities, totalling 200 fruits per area. the physical variables of the fruits and pits, the number of little fruits (frutilhos), pits and seeds, and the percentage of intact and damaged fruits and pits were determined. the results indicated that there are physical differences among the collected areas, with the region of japonvar having the most vigorous pits, with the lowest rates of attack by the pequi fruit borer. in addition, skin thickness and length of fruits from japonvar and mirabela, respectively, had relations with the percentage of attack by carmenta sp. these differences found among the areas may be associated with the climatic and edaphic influences of these regions, which may also highlight the linkage to the genetical composition aspects of the pequi.
The study of vancomycin use and its adverse reactions associated to patients of a brazilian university hospital
Daniel Marinho, Gisele Huf, Bruno LA Ferreira, Helena Castro, Carlos R Rodrigues, Valeria Sousa, Lúcio M Cabral
BMC Research Notes , 2011, DOI: 10.1186/1756-0500-4-236
Abstract: A cross-sectional retrospective study was carried out among inpatients that received vancomycin in a Brazilian quaternary hospital. The occurrence of adverse reactions reported was evaluated in medical records relating to patients taking vancomycin during a one year period. Males comprised 52% (95% CI: 41.7-60.2%) of the sample population, with a mean age of 50.6 (95% CI: 47.2-54.0) years and mean treatment period of 9.7 (95% CI: 8.0-11.5) Days. It was verified that nephrotoxicity occurred in 18.4% (95% CI: 11.3-27.5) of patients, Red man syndrome occurred in 2% (95% CI 0.2-7.2), while the occurrence of thrombocytopenia was 7.1% (95% CI: 2.9-14.2).It may be noted that even after 50 years of use, adverse reactions associated with vancomycin continue with high frequency, presenting a public health problem, especially considering its current use in cases of multidrug resistant infections. In this context, we emphasize the importance of intensive pharmacovigilance in hospital as a surveillance tool after drug approval by the sanitary authority.Vancomycin is a complex glycopeptide antibiotic with an unusual structure and molecular mass of approximately 1500 Da. Vancomycin acts primarily inhibiting the cell-wall biosynthesis. Its action against gram-positive cell walls is related to the prevention of the incorporation of N-acetylmuramic acid and N-acetylglucosamine into the peptideoglycan matrix. Its therapeutic use against infections caused by gram-positive bacteria started in the 1950's and it is still used currently. During the 1960's and 70's its use suffered a large decline, due to the high incidence of adverse reactions and the introduction of semisynthetic penicillins with broadest spectrum of action in the therapeutic arsenal [1]. However, the rapid development of bacterial resistance to semisynthetic penicillin, especially methicillin-resistant Staphylococcus aureus (MRSA), led to the return of the use of vancomycin on a large scale from the 1980's.During the fol
O Uso da Contabilidade Estratégica nas Organiza es: um Modelo de Análise Estratégica para o Departamento de Contabilidade
Gisele de Souza Castro Vieira,Rafael Borges Morch,Ricardo Antonio Torres Rodrigues,Aracéli Cristina De Sousa Ferreira
Revista de Educa??o e Pesquisa em Contabilidade , 2010,
Abstract: O objetivo deste estudo é, com o uso da ferramenta de análise SWOT, propor uma metodologia para o departamento de contabilidade que possa auxiliá-lo no diagnóstico e na formula o de uma estratégia e na redefini o de seu posicionamento na administra o da empresa. Trata-se de um estudo teórico e bibliográfico no qual se pretende discutir como as técnicas de administra o estratégica para a análise do ambiente podem ser aplicadas na contabilidade de uma empresa. Espera-se que o modelo proposto, que combina métodos de análise com as quest es elaboradas a partir da análise das variáveis internas e externas, auxilie o alcance satisfatório dos objetivos propostos por departamentos de contabilidade comprometidos com o processo de reposicionamento e adapta o aos preceitos da contabilidade estratégica.
Continuing Education for Professional Development in Higher Education Teaching  [PDF]
Jacques de Lima Ferreira, Gisele Rietow Bertotti
Creative Education (CE) , 2016, DOI: 10.4236/ce.2016.710148
Abstract: This paper presents a qualitative research of the case study type that was held with professionals from various fields of knowledge (law, nursing, administration, education, accounting, medicine, physical therapy, journalism, economics, engineering, etc.), undergraduates who participated in a continuing education course, specifically, a specialization in continuing education to work in higher education in a community institution. The research problem that guided the research was: What are the contributions, implications and impacts of continuing education for teaching in higher education from the perceptions of professionals from various fields of knowledge? The research aimed to analyze the professionals’ repercussions of the process of continuing education for the practice of teaching in higher education. The research involved three classes of specialization of the following years: 2013, with 25 students; 2014 with 22 students and 2015 with 23 students, totaling 70 subjects investigated. Data were collected from a questionnaire with open and closed questions which has enabled content analysis from the perspective of Bardin (2011) with the help of Atlas Ti software. The results showed that the participation in the continuing education course contributed to teaching in higher education through the possibility of obtaining pedagogical knowledge and practical application of these, which culminated in a reflection on pedagogical practice, impacting directly on the professional work of these teachers, who only felt the lack of a more detailed work with the education of the distance mode.
Fracture strength of composite fixed partial denture using bovine teeth as a substitute for human teeth with or without fiber-reinforcement
Soares, Carlos José;Barbosa, Liliane Minglini;Santana, Fernanda Ribeiro;Soares, Priscilla Barbosa Ferreira;Mota, Adérito Soares da;Silva, Gisele Rodrigues da;
Brazilian Dental Journal , 2010, DOI: 10.1590/S0103-64402010000300011
Abstract: this study evaluate the use of bovine teeth as a substitute for human teeth on fracture strength tests of composite fixed partial dentures (cpd), with and without fiberglass reinforcement (fg). eighty teeth were selected, being 40 bovine incisors, 20 human premolars and 20 molars. bovine incisors were ground to get a platform, simulating an occlusal surface of human molar. teeth in pairs were embedded in polystyrene resin, simulating the periodontal ligament and divided in 4 groups: b-cpd-fg: bovine teeth restored with cpd with fg; b-cpd-nfg: bovine teeth restored with cpd without fg; h-cpd-fg: human teeth restored cpd with fg; and h-cpd-nfg: human teeth restored with cpd without fg. the cpd were adhesively fixed and submitted to an axial compression load at the pontic center with a crosshead speed of 0.5 mm/min until fracture. failure modes were assessed and classified. data were subjected to two-way anova and tukey's hsd test (?=0.05). the tooth type had no influence on fracture strength and fracture mode. the inclusion of fiberglass increased significantly the fracture strength. the failure modes were more reparable in groups with fiber-reinforcement. bovine teeth can be used as a substitute for human teeth in these types of fracture strength tests.
Características físico-quimicas, análise sensorial e conserva??o de frutos de cultivares de bananeira resistentes à sigatoca-negra
Siqueira, Célia Lúcia;Rodrigues, Maria Luisa Mendes;Mizobutsi, Gisele Polete;Santos, Paulo Gomes dos;Mota, Wagner Ferreira da;Mizobutsi, Edson Hiydu;Oliveira, Gildete Bispo de;
Revista Ceres , 2010, DOI: 10.1590/S0034-737X2010000500017
Abstract: the cultivar prata an? is widely grown in the north of minas gerais state, brazil, hence, the banana business in the region is based on a variety susceptible to black sigatoka. although there are resistant cultivars being recommended to cultivation in the region, studies related to post-harvest conservation are still scarce. the objective of the present work was to evaluate the storage period and physiochemical and sensorial characteristics of cultivars, fhia - 02 and preciosa, both resistant to black sigatoka, and the effect of the modified atmosphere associated with cooling. three experiments were carried out, the first under ambient temperature, the second at 12oc and the third at 15oc, the experiments were arranged in randomized block design with split-plots in the time, having in the plots a 2x2 factorial (with and without packing) x (fhia - 02 and preciosa), and in the subplots the evaluations at six and two days, at 12, 15 and 25o c, with four repetitions and three fruits per experimental unit. after the treatment, the fruits were placed in expanded polystyrene trays (without packaging) and 10μm and 16μm packagings and relative humidity of 90 %. the evaluated characteristics were size, diameter, color, firmness, loss of fresh matter, total soluble solids (tss), total titratable acidity (tta), tss/tta, ph and sensorial analysis. the use of packaging associated with cooling allowed fruit storage for 28 days keeping its physical-chemical characteristics.
Desenvolvimento institucional e o envolvimento do setor privado na provis?o de infra-estrutura
Tiryaki, Gisele Ferreira;
Economia Aplicada , 2008, DOI: 10.1590/S1413-80502008000300007
Abstract: private participation in infrastructure grew significantly in developing countries during the 1990s, although a slowdown in investment activity has become evident since the asian crisis in 1997. investment in infrastructure involves a significant volume of sunk costs on long-lived assets and risks associated with government's engagement in opportunistic holdup. in this context, macroeconomic, political and institutional instability constitute obstacles to a greater private sector envolvement in infraestructure projects. using the generalized method of moments methodology, this article presents empirical evidence that the flow of resources to infrastructure projects with private participation is greater in those coutries with a better institucional environment.
A informalidade e as flutua??es na atividade econ?mica
Tiryaki, Gisele Ferreira;
Estudos Econ?micos (S?o Paulo) , 2008, DOI: 10.1590/S0101-41612008000100005
Abstract: previous theoretical and empirical works have focused on the impact of an enlarging informal sector on growth. this paper shows that the size of the informal economy also affects business cycles volatility. informal businesses are usually small in size, which prevents them from achieving economies of scale, from operating with the right capital/labor mix, and from having access to credit markets. this lack of access to credit markets hinders informal firms' ability to neutralize the cash flow squeeze arising during recessions, increasing their exposure to fluctuations in economic activity and their likelihood of going bankrupt. using a generalized method of moments methodology on a cross-country dataset, this paper shows that the larger the informal sector of a country, the greater the volatility experienced in output, investment and consumption over the business cycles.
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