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Search Results: 1 - 10 of 2948 matches for " Geum-Sook Hwang "
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Metabolomics approach for discovering disease biomarkers and understanding metabolic pathway
Jeeyoun Jung,Zihey Jang,Geum-Sook Hwang
Journal of Analytical Science & Technology , 2011,
Abstract: Metabolomics, the multi-targeted analysis of endogenous metabolites from biological samples, can be efficiently applied to screen disease biomarkers and investigate pathophysiological processes. Metabolites change rapidly in response to physiological perturbations, making them the closest link to disease phenotypes. This study explored the role of metabolomics in gaining mechanistic insight into disease processes and in searching for novel biomarkers of human diseases
1H NMR-Based Metabolite Profiling of Planktonic and Biofilm Cells in Acinetobacter baumannii 1656-2
Jinki Yeom, Ji-Hyun Shin, Ji-Young Yang, Jungmin Kim, Geum-Sook Hwang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0057730
Abstract: Acinetobacter baumannii is an aerobic and gram-negative pathogenic bacterium that is resistant to most antibiotics. Recently, A. baumannii 1656-2 exhibited the ability to form biofilms under clinical conditions. In this study, global metabolite profiling of both planktonic and biofilm forms of A. baumannii 1656-2 was performed using high-resolution nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analysis to investigate the metabolic patterns leading to biofilm formation. Principal components analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots showed a distinct separation between planktonic and biofilm cells. Metabolites including acetates, pyruvate, succinate, UDP-glucose, AMP, glutamate, and lysine were increasingly involved in the energy metabolism of biofilm formation. In particular, the ratio of N-acetyl-D-glucosamine (GlcNAc) to D-glucosamine (GlcNH2) was significantly higher during biofilm formation than under the planktonic condition. This study demonstrates that NMR-based global metabolite profiling of bacterial cells can provide valuable insight into the metabolic changes in multidrug resistant and biofilm-forming bacteria such as A. baumannii 1656-2.
1H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease
Ju-Ae Kim, Hyo-Jung Choi, Yong-Kook Kwon, Do Hyun Ryu, Tae-Hwan Kwon, Geum-Sook Hwang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0085445
Abstract: Chronic kidney disease (CKD) is characterized by the gradual loss of the kidney function to excrete wastes and fluids from the blood. 1H NMR-based metabolomics was exploited to investigate the altered metabolic pattern in rats with CKD induced by surgical reduction of the renal mass (i.e., 5/6 nephrectomy (5/6 Nx)), particularly for identifying specific metabolic biomarkers associated with early of CKD. Plasma metabolite profiling was performed in CKD rats (at 4- or 8-weeks after 5/6 Nx) compared to sham-operated rats. Principle components analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) score plots showed a significant separation between the groups. The resulting metabolic profiles demonstrated significantly increased plasma levels of organic anions, including citrate, β-hydroxybutyrate, lactate, acetate, acetoacetate, and formate in CKD. Moreover, levels of alanine, glutamine, and glutamate were significantly higher. These changes were likely to be associated with complicated metabolic acidosis in CKD for counteracting systemic metabolic acidosis or increased protein catabolism from muscle. In contrast, levels of VLDL/LDL (CH2)n and N-acetylglycoproteins were decreased. Taken together, the observed changes of plasma metabolite profiles in CKD rats provide insights into the disturbed metabolism in early phase of CKD, in particular for the altered metabolism of acid-base and/or amino acids.
Gender-Specific Metabolomic Profiling of Obesity in Leptin-Deficient ob/ob Mice by 1H NMR Spectroscopy
Eun-Young Won, Mi-Kyung Yoon, Sang-Woo Kim, Youngae Jung, Hyun-Whee Bae, Daeyoup Lee, Sung Goo Park, Chul-Ho Lee, Geum-Sook Hwang, Seung-Wook Chi
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0075998
Abstract: Despite the numerous metabolic studies on obesity, gender bias in obesity has rarely been investigated. Here, we report the metabolomic analysis of obesity by using leptin-deficient ob/ob mice based on the gender. Metabolomic analyses of urine and serum from ob/ob mice compared with those from C57BL/6J lean mice, based on the 1H NMR spectroscopy in combination with multivariate statistical analysis, revealed clear metabolic differences between obese and lean mice. We also identified 48 urine and 22 serum metabolites that were statistically significantly altered in obese mice compared to lean controls. These metabolites are involved in amino acid metabolism (leucine, alanine, ariginine, lysine, and methionine), tricarbocylic acid cycle and glucose metabolism (pyruvate, citrate, glycolate, acetoacetate, and acetone), lipid metabolism (cholesterol and carnitine), creatine metabolism (creatine and creatinine), and gut-microbiome-derived metabolism (choline, TMAO, hippurate, p-cresol, isobutyrate, 2-hydroxyisobutyrate, methylamine, and trigonelline). Notably, our metabolomic studies showed distinct gender variations. The obese male mice metabolism was specifically associated with insulin signaling, whereas the obese female mice metabolism was associated with lipid metabolism. Taken together, our study identifies the biomarker signature for obesity in ob/ob mice and provides biochemical insights into the metabolic alteration in obesity based on gender.
1H NMR-based metabolite profiling of diet-induced obesity in a mouse mode
Jee-youn Jung1,2,#, Il Yong Kim3,#, Yo Na Kim3,#, Jin-sup Kim1,5, Jae Hoon Shin3, Zi-hey Jang1,5, Ho-Sub Lee2, Geum-Sook Hwang1,5,* & Je Kyung Seong3,4,*
BMB Reports , 2012,
Abstract: High-fat diets (HFD) and high-carbohydrate diets (HCD)-induced obesity through different pathways, but the metabolicdifferences between these diets are not fully understood.Therefore, we applied proton nuclear magnetic resonance (1HNMR)-based metabolomics to compare the metabolic patternsbetween C57BL/6 mice fed HCD and those fed HFD. Principalcomponent analysis derived from 1H NMR spectra of urineshowed a clear separation between the HCD and HFD groups.Based on the changes in urinary metabolites, the slow rate ofweight gain in mice fed the HCD related to activation of thetricarboxylic acid cycle (resulting in increased levels of citrateand succinate in HCD mice), while the HFD affected nicotinamidemetabolism (increased levels of 1-methylnicotineamide,nicotinamide-N-oxide in HFD mice), which leads to systemicoxidative stress. In addition, perturbation of gut microflorametabolism was also related to different metabolic patterns ofthose two diets. These findings demonstrate that 1H NMRbasedmetabolomics can identify diet-dependent perturbationsin biological pathways.
Effects of a Health Promotion Program on Elementary Students’ Physical and Mental Health  [PDF]
Insook Lee, Kyung-Sook Bang, Sungjae Kim, Heeseung Choi, Inju Hwang
Open Journal of Nursing (OJN) , 2015, DOI: 10.4236/ojn.2015.59085
Abstract: Many health promotion programs have been implemented to prevent obesity and mental health problems among school-aged children. However, only a few programs included both physical and psychological measures to assess the effects of the program. The present study was designed to test the effects of a 6-week health promotion program on physical and mental health among school-aged children using physiological and psychological measures. A total of 74 elementary school students (4th and 5th grade) recruited from urban (n = 51) and rural areas (n = 23) participated in the study. We used the Korean version of the Kovacs’ Children’s Depression Inventory and the Conners-Wells’ Adolescent Self-Report Scale-Short Form (CASS-S) to measure levels of depression and hyperactivity, respectively. To assess children’s physical health, we measured body mass index (BMI), body fat percentage, and heart rate variability. In addition, children’s health-related behaviors (e.g., eating and exercise habits) were assessed using the health promotion behavior scale. A total of 63 students (13 in the experimental and 50 in the control group) who completed both physiological and psychological measures were included in the final analysis. Compared to children in the control group, children in the experimental group showed a significant decrease in their level of hyperactivity (t = -2.01, p = 0.049) and increase in heart rate variability (t = -2.35, p = 0.022). No significant group differences were noted for depression, BMI, body fat percentage, and health promotion behaviors between the two groups. Findings of this study provided preliminary evidence of the effectiveness of a 6-week school-based health promotion program for improving emotional health among school-aged children living in a rural area. Future studies testing the effects of health promotion programs with longer sessions and programs focusing on social-emotional skill development are needed.
Effects of coffee, smoking, and alcohol on liver function tests: a comprehensive cross-sectional study
Jang Eun,Jeong Sook-Hyang,Hwang Sung,Kim Hyun
BMC Gastroenterology , 2012, DOI: 10.1186/1471-230x-12-145
Abstract: Background Liver function tests (LFTs) can be affected by many factors and the proposed effects of coffee on LFT require a comprehensive evaluation. The aim of this study was to elucidate whether drinking coffee, smoking, or drinking alcohol have independent effects on LFTs in Korean health-check examinees. Methods We used the responses of 500 health-check examinees, who had participated in a self-administered questionnaire survey about coffee, alcohol drinking, and smoking habits. Results Coffee consumption was closely related to male gender, high body mass index (BMI), alcohol drinking, and smoking. On univariable and multivariable analyses, drinking coffee lowered serum levels of total protein, albumin, and aspartate aminotransferases (AST). On multivariable analyses, smoking raised serum γ-glutamyl transferase (GGT) level and decreased serum protein and albumin levels, while alcohol drinking raised GGT level after adjustment for age, gender, regular medication, BMI, coffee and alcohol drinking amounts, and smoking. Conclusions Coffee consumption, smoking, and alcohol drinking affect the individual components of LFT in different ways, and the above 3 habits each have an impact on LFTs. Therefore, their effects on LFTs should be carefully interpreted, and further study on the mechanism of the effects is warranted.
Covalent NEDD8 Conjugation Increases RCAN1 Protein Stability and Potentiates Its Inhibitory Action on Calcineurin
Eun Hye Noh, Hee Sook Hwang, Hee Sun Hwang, Boram Min, Eunju Im, Kwang Chul Chung
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0048315
Abstract: Similar to ubiquitin, regulatory roles for NEDD8 (neural precursor cell-expressed developmentally down-regulated 8) are being clarified during cell growth, signal transduction, immune response, and development. However, NEDD8 targets and their functional alterations are not well known. Regulator of calcineurin 1 (RCAN1/DSCR1P1) is located near the Down syndrome critical region on the distal part of chromosome 21, and its gene product is an endogenous inhibitor of calcineurin signaling. RCAN1 is modified by ubiquitin and consequently undergoes proteasomal degradation. Here we report that NEDD8 is conjugated to RCAN1 (RCAN1-1S) via three lysine residues, K96, K104, and K107. Neddylation enhances RCAN1 protein stability without affecting its cellular location. In addition, we found that neddylation significantly inhibits proteasomal degradation of RCAN1, which may underlie the ability of NEDD8 to enhance RCAN1 stability. Furthermore, neddylation increases RCAN1 binding to calcineurin, which potentiates its inhibitory activity toward downstream NFAT signaling. The present study provides a new regulatory mechanism of RCAN1 function and highlights an important role for diverse RCAN1-involved cellular physiology.
Neuroprotective Effects of Herbal Ethanol Extracts from Gynostemma pentaphyllum in the 6-Hydroxydopamine-Lesioned Rat Model of Parkinson's Disease
Hyun Sook Choi,Mi Sook Park,Seung Hwan Kim,Bang Yeon Hwang,Chong Kil Lee,Myung Koo Lee
Molecules , 2010, DOI: 10.3390/molecules15042814
Abstract: 6-Hydroxydopamine administration for 28 days (8 μg/2 μL) reduced the number of tyrosine hydroxylase (TH)-immunopositive neurons to 40.2% in the substantia nigra compared to the intact contralateral side. Dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and norepinephrine levels were reduced to 19.1%, 52.3%, 47.1% and 67.4% in the striatum of 6-hydroxydopamine-lesioned rats compared to the control group, respectively. However, an oral administration of herbal ethanol extracts from Gynostemma pentaphyllum (GP-EX) (10 mg/kg and 30 mg/kg) starting on day 3 post-lesion for 28 days markedly ameliorated the reduction of TH-immunopositive neurons induced by 6-hydroxydopamine-lesioned rat brain from 40.2% to 67.4% and 75.8% in the substantia nigra. GP-EX administration (10 and 30 mg/kg) also recovered the levels of dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and norepinephrine in post-lesion striatum to 64.1% and 65.0%, 77.9% and 89.7%, 82.6% and 90.2%, and 88.1% and 89.2% of the control group. GP-EX at the given doses did not produce any sign of toxicity such as weight loss, diarrhea and vomiting in rats during the 28 day treatment period and four gypenoside derivatives, gynosaponin TN-1, gynosaponin TN-2, gypenoside XLV and gypenoside LXXIV were identified from GP-EX. These results suggest that GP-EX might be helpful in the prevention of Parkinson’s disease.
Proton Pump Inhibitors Exert Anti-Allergic Effects by Reducing TCTP Secretion
Sunghee Choi,Hyun Jung Min,Miyoung Kim,Eun Sook Hwang,Kyunglim Lee
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0005732
Abstract: Extracellular translationally controlled tumor protein (TCTP) is known to play a role in human allergic responses. TCTP has been identified outside of macrophages, in activated mononuclear cells, and in biological fluids from allergic patients. Even TCTP devoid of signal sequences, is secreted to extracellular environment by an yet undefined mechanism. This study is aimed at understanding the mechanism of TCTP release and its regulation. A secondary goal is to see if inhibitors of TCTP release can serve as potential anti-allergic asthmatic drugs.
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