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Search Results: 1 - 10 of 1247 matches for " Gert Auer "
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Multivariate meta-analysis of proteomics data from human prostate and colon tumours
Lina Rosenberg, Bo Franzén, Gert Auer, Janne Lehti?, Jenny Forshed
BMC Bioinformatics , 2010, DOI: 10.1186/1471-2105-11-468
Abstract: We here demonstrate a multivariate meta-analysis of 2DE proteomics data from human prostate and colon tumours. We developed a bioinformatic workflow for identifying common patterns over two tumour types. This included dealing with pre-processing of data and handling of missing values followed by the development of a multivariate Partial Least Squares (PLS) model for prediction and variable selection. The variable selection was based on the variables performance in the PLS model in combination with stability in the validation. The PLS model development and variable selection was rigorously evaluated using a double cross-validation scheme. The most stable variables from a bootstrap validation gave a mean prediction success of 93% when predicting left out test sets on models discriminating between normal and tumour tissue, common for the two tumour types. The analysis conducted in this study identified 14 proteins with a common trend between the tumour types prostate and colon, i.e. the same expression profile between normal and tumour samples.The workflow for meta-analysis developed in this study enabled the finding of a common protein profile for two malign tumour types, which was not possible to identify when analysing the data sets separately.Emerging molecular pictures of cancer makes it evident that cancer portrays a group of diseases with numerous mutations and effected cellular pathways cross many different tumour types. The same permuted pathways drive tumour growth and metastasis in various cancer types; however some of the molecular features are tissue and tumour type specific. The key to personalized cancer therapy is the ability to classify and characterize tumours by molecular features to then be able to select the therapy [1]. Proteome analysis can provide valuable phenotypic information of tumour tissue on the molecular level. Proteomics, the global analysis of a proteome, has contributed to the understanding of global protein changes in cells [2].Two-d
Expression of Cyclins A, E and Topoisomerase II α correlates with centrosome amplification and genomic instability and influences the reliability of cytometric S-phase determination
Ulrike Kronenwett, Juan Castro, Uwe J Roblick, Kaoru Fujioka, Carin ?string, Farinaz Faridmoghaddam, Nongnit Laytragoon-Lewin, Bernhard Tribukait, Gert Auer
BMC Cell Biology , 2003, DOI: 10.1186/1471-2121-4-8
Abstract: Our results demonstrated a correlation between centrosome amplification, cell cycle fidelity and the level of mRNA and protein expression of cyclins A and E during the part of the cell cycle defined as G1-phase by means of DNA content based histogram analysis. It is shown that the normal diploid breast cell line HTB-125, the genomically relatively stable aneuploid breast cancer cell line MCF-7, and the genomically unstable aneuploid breast cancer cell line MDA-231 differ remarkably concerning both mRNA and protein expression of the two cyclins during G1-phase. In MDA-231 cells the expression of e.g. cyclin A mRNA was found to be ten times higher than in MCF-7 cells and about 500 times higher than in HTB-125 cells. Topoisomerase II α showed high mRNA expression in MDA compared to MCF-7 cells, but the difference in protein expression was small. Furthermore, we measured centrosome aberrations in 8.4% of the MDA-231 cells, and in only 1.3% of the more stable aneuploid cell line MCF-7. MDA cells showed 27% more incorporation of BrdU than reflected by S-phase determination with flow cytometric DNA content analysis, whereas these values were found to be of the same size in both HTB-125 and MCF-7 cells.Our data indicate that the breast cancer cell lines MCF-7 and MDA-231, although both DNA-aneuploid, differ significantly regarding the degree of cell cycle disturbance and centrosome aberrations, which partly could explain the different genomic stability of the two cell lines. The results also question the reliability of cytometric DNA content based S-phase determination in genomically unstable tumor cell populations.The centrosome is the major microtubule-organising centre in mammalian cells and is a principal part of the cytoskeleton. It consists of two barrel-shaped centrioles surrounded by pericentriolar material [1] and reproduces during interphase of the cell cycle. During mitosis the centrosome functions as spindle pole, assembling spindle microtubules to guarantee cor
Intrinsic molecular signature of breast cancer in a population-based cohort of 412 patients
Stefano Calza, Per Hall, Gert Auer, Judith Bj?hle, Sigrid Klaar, Ulrike Kronenwett, Edison T Liu, Lance Miller, Alexander Ploner, Johanna Smeds, Jonas Bergh, Yudi Pawitan
Breast Cancer Research , 2006, DOI: 10.1186/bcr1517
Abstract: We obtained gene expression and clinical data for 412 breast cancers obtained from population-based cohorts of patients from Stockholm and Uppsala, Sweden. Using the intrinsic set of approximately 500 genes derived in the Norway/Stanford breast cancer data, we validated the existence of five molecular subtypes – basal-like, ERBB2, luminal A/B and normal-like – and characterized these subtypes extensively with the use of conventional clinical variables.We found an overall 77.5% concordance between the centroid prediction of the Swedish cohort by using the Norway/Stanford signature and the k-means clustering performed internally within the Swedish cohort. The highest rate of discordant assignments occurred between the luminal A and luminal B subtypes and between the luminal B and ERBB2 subtypes. The subtypes varied significantly in terms of grade (p < 0.001), p53 mutation (p < 0.001) and genomic instability (p = 0.01), but surprisingly there was little difference in lymph-node metastasis (p = 0.31). Furthermore, current users of hormone-replacement therapy were strikingly over-represented in the normal-like subgroup (p < 0.001). Separate analyses of the patients who received endocrine therapy and those who did not receive any adjuvant therapy supported the previous hypothesis that the basal-like subtype responded to adjuvant treatment, whereas the ERBB2 and luminal B subtypes were poor responders.We found that the intrinsic molecular subtypes of breast cancer are broadly present in a diverse collection of patients from a population-based cohort in Sweden. The intrinsic gene set, originally selected to reveal stable tumor characteristics, was shown to have a strong correlation with progression-related properties such as grade, p53 mutation and genomic instability.A significant reduction of disease burden from breast cancer can be achieved by a better characterization of its potentially fatal forms. Spread of the disease, cell structure, differentiation and growth patte
The integration of functional genomics, combinatorial chemistry and nanotechnology into a miniaturized drug discovery process
M Auer
Arthritis Research & Therapy , 2001, DOI: 10.1186/ar153
Abstract: Single molecule fluorescence spectroscopy and confocal nanoscanning/AIDA technology provide an optimal combination for a miniaturized automated uHTS process with high mechanistic resolution in functional and coupled assay systems.
Short Note on Correlation in a Hull-White Tree
Martin Auer
Lecture Notes in Engineering and Computer Science , 2009,
Entre assimilation et exclusion: La représentation du Highlander dans la presse des Basses Terres d’Ecosse au milieu du dix-neuvième siècle
Christian Auer
Synergies Royaume-Uni et Irlande , 2008,
Abstract: Au cours des premières décennies du dix-neuvième siècle, la paysannerie des Hautes Terres d’Ecosse connut une profonde et douloureuse mutation sous l’impulsion de divers facteurs culturels et économiques. La presse des Basses Terres se fit l’écho de ces transformations et se montra le plus souvent critique envers les habitants d’une région per ue comme étrangère. L’analyse du discours du Blackwood’s Edinburgh Magazine, l’une des publications littéraires majeures en Grande-Bretagne, et de deux des journaux qui firent preuve de la plus grande virulence à l’encontre des Highlanders, le Perthshire Advertiser et le Fifeshire Journal, permet d’identifier la nature des stratégies de distanciation auxquelles la presse des Basses Terres eut recours. Dans cet article je m’efforcerai de démontrer que les analyses proposées par Edward Said dans Orientalism et Culture and Imperialism peuvent s’appliquer à la description des mécanismes mis en uvre par la presse des Lowlands.Said, en effet, assimile le discours colonial à un système statique d’essentialisme synchronique . Or le discours de la presse des Lowlands avait comme vocation première de présenter la communauté des Highlands comme essentiellement statique, de la rejeter dans l’altérité et d’en discréditer les schémas culturels. La perception fondamentalement négative de la celtitude s’articulait autour d’un ensemble de couples antithétiques (travail-indolence, supériorité-infériorité, modernité-archa sme ou encore civilisationbarbarie). Une démarche de type ethnocidaire s’imposait donc afin que le Highlander puisse quitter son statut d’étranger pour bénéficier, au même titre que les autres membres de la communauté britannique, des bienfaits de la civilisation et du progrès.
Klinische Studie/Klinische Praxis: Kann AT-I-Rezeprotblockade das Risiko für Restenose nach Implantation von "Bare-metal" Stents in Koronararterien reduzieren?
Auer J
Zeitschrift für Gef??medizin , 2009,
Update: Therapie der chronischen Herzinsuffizienz
Auer J
Journal für Kardiologie , 2009,
Abstract: Die Herzinsuffizienz stellt eine der h ufigsten internistischen Erkrankungen dar. Grundpfeiler der pharmakologischen Therapie der chronischen Herzinsuffizienz mit dem Ziel einer Prognoseverbesserung ist die Blockade der im Rahmen des Syndroms Herzinsuffizienz stattfindenden neurohumoralen Aktivierung. Die Ger tetherapie (CRT, ICD) gewinnt im Therapiekonzept der Behandlung von Patienten mit chronischer Herzinsuffizienz zunehmende Bedeutung. Effektive Therapien etwa für spezifische Behandlung der diastolischen Herzinsuffizienz sind limitiert. Interessante neue Therapieans tze zur Behandlung der chronischen Herzinsuffizienz werden gegenw rtig in klinischen Studien untersucht. Gro e klinische Untersuchungen mit neuen Substanzen brachten zum Teil gemischte Ergebnisse. Das unterstreicht die Tatsache, dass es schwierig ist, zus tzlich zur momentan verfügbaren Therapie einen additiven Benefit zu erreichen. Die folgende Arbeit gibt eine übersicht, was sich auf dem Gebiet der Herzinsuffizienztherapie in den vergangenen Jahren entwickelt hat.
Klinische Studien/Klinische Praxis: Kann AT-I-Rezeptorblockade das Risiko für Restenose nach Implantation von "Bare-metal" Stents in Koronararterien reduzieren!
Auer J
Journal für Kardiologie , 2009,
Aus dem Herzkatheterlabor: Transientes linksventrikul res "Apical Ballooning" als Differentialdiagnose zum akuten Myokardinfarkt
Auer J
Journal für Kardiologie , 2004,
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