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Search Results: 1 - 10 of 317898 matches for " Geraldo Célio Brand?o "
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High Resolution Mass Spectrometry Elucidation of Captopril’s Ozonation and Chlorination By-Products  [PDF]
Frederico Jehár Oliveira Quint?o, Geraldo Célio Brando, Silvana de Queiroz Silva, Sérgio Francisco Aquino, Robson José de Cássia Franco Afonso
American Journal of Analytical Chemistry (AJAC) , 2017, DOI: 10.4236/ajac.2017.84020
Abstract: The article evaluated the degradation of the captopril in aqueous solution after ozonation and chlorination. The process was continuously monitored focusing on the identification, mass spectrometry and elucidation of its by-products by applying direct infusion and high performance liquid chromatography, electrospray ionization high resolution mass spectrometry, in the negative ion mode. The cytotoxicity of its by-products solutions were evaluated with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. It was observed through that after 30 min of ozonation and chlorination, there was complete oxidation of captopril, i.e., 100% removal efficiency. At these conditions, the rate of mineralization, by total organic carbon, was only 7.63% for ozonation and 6.40% for chlorination, evidencing the formation of degradation by-products. Ten captopril by-products were identified and their respective chemical structures elucidations are proposed. The treated samples and their by-products were nontoxic to HepG2 cells by MTT assay.
Synthesis of triazol derivatives of lupeol with potential antimalarial activity Síntese de derivados triazólicos com potencial atividade antimalárica a partir do lupeol
Tatiane Freitas Borgati,Guilherme Rocha Pereira,Geraldo Célio Brando,Alaíde Braga de Oliveira
Orbital : the Electronic Journal of Chemistry , 2012,
Abstract: The goal of this project is synthesize and characterization of derivatives of lupeol and evaluated antimalarial activity. Historically, plants are important source of antimalarial medicines, highlighting quinine (1) (Figure 1), an important alkaloid from the Cinchona calisaya bark. This compound was an important model for cloroquine synthesis, a drug that was widely used in malaria treatment. In addition, one of the principal medicines used today is artemisinine, isolated from the Chinese plant Artemisia annua L (2) (Figure 1), and their semi synthetic derivatives (artesunate, artemeter, arteter). However, the malaria parasite has already shown resistance to most of these current drugs and the search for new candidates is essential. Lupeol (3) (Figura 1) is a compound that occurs in many plant species and discloses antimalarial, antiinflamatoryl and antitumoral activities. Considering its potential as a lead antimalarial molecule, we focused our work in the synthesis of new lupeol derivatives with increased antimalarial activity(scheme 1). Relata-se nesse trabalho a síntese e caracteriza o de derivados do luepol para avalia o da atividade antimalárica. Historicamente, as plantas s o uma importante fonte de fármacos antimaláricos destacando-se a quinina (1) (Figura 1), um alcalóide extraído da casca da Cinchona calisaya, que serviu de modelo para síntese da cloroquina, um dos fármacos que foi mais amplamente empregado no tratamento da malária. Atualmente, os principais fármacos em uso clínico s o a artemisinina, isolada da planta chinesa Artemisia annua L. (2) (Figura 1), e seus derivados semi-sintéticos (artesunato, artemeter, arteter). A busca de novos antimaláricos é de alta relevancia, uma vez que já se registrou resistência do P. falciparum à artemisinina e derivados. Sabe-se que o lupeol (3) (Figura 1) ocorre em várias espécies vegetais e possui atividades antimalárica, anti-inflamatória e antitumoral. Dessa forma, ele foi escolhido como material de partida para a síntese de novas drogas com potencial atividade antimalárica. Prop e-se com esse projeto modifica es químicas no lupeol, incluindo a incorpora o da 7- cloroquinolina à estrutura deste, via síntese de híbridos triazólicos. Os métodos de síntese utilizados s o fundamentados em rea es clássicas da química organica (Esquema 1) como, substitui o nucleofílica, e a cicloadi o entre um alcino terminal e uma azida organica catalisada por cobre, também conhecida como “rea o click “ (CuAAC). As substancias foram sintetizadas com rendimentos variando entre 40 e 80%. Os produtos obt
Antiviral activities of plants occurring in the state of Minas Gerais, Brazil: Part 2. Screening Bignoniaceae species Atividade antiviral de extratos de plantas coletadas no estado de Minas Gerais: Parte 2. Triagem de Bignoniaceae
Geraldo Célio Brando,Erna G. Kroon,Jo?o Rodrigues dos Santos,Jo?o Renato Stehmann
Revista Brasileira de Farmacognosia , 2010,
Abstract: Ethanol extracts of eighteen Bignoniaceae species have been evaluated by the MTT assay for cytotoxicity in Vero cells and for antiviral activity against Human herpes virus type 1, Vaccinia virus and murine Encephalomyocarditis virus. Among such species, seven are reported to be of traditional medicinal use No cytotoxicity was observed for most of the extracts up to the concentration of 500 μg/mL. Fourteen (50%) of the 28 extracts assayed have disclosed antiviral activity with EC50 values in the range of 4.6+0.3 to 377.2+17.7 μg/mL. Only two species, Arrabidaea samydoides and Callichlamys latifolia, have shown activity against all the three viruses. The extracts were chemically characterized by their TLC and HPLC-DAD profiles. Mangiferin is the major constituent of A. samydoides but the isolated compound has been less active than the crude extract. This is the first report on the antiviral evaluation of the eighteen Bignoniaceae species assayed. Extratos etanólicos de dezoito espécies vegetais pertencentes à família Bignoniaceae, das quais sete s o descritas como de uso medicinal, foram avaliados, pelo ensaio colorimétrico do MTT, para atividades citotóxica, em células Vero, e antiviral, frente aos vírus herpes simplex-tipo 1, vaccinia e encefalomiocardite murina. A maior parte dos extratos n o apresentou citotoxicidade até a concentra o de 500 μg/mL. Dos 28 extratos testados quatorze (50%) apresentaram atividade antiviral com valores de CE50 na faixa de 4,6+03 a 377,2+17,7 μg/mL. Somente duas espécies, Arrabidaea samydoides e Callichlamys latifolia, foram ativas frente aos três vírus. Os extratos foram caracterizados pelos seus perfís cromatográficos em CCD e CLAE-FR. Análises por CLAE-FR mostraram que a mangiferina é o constituinte majoritário em A. samydoides mas a substancia isolada foi menos ativa do que o extrato bruto. Esta é a primeira vez que se relata a atividade antiviral de extratos das dezoito espécies avaliadas.
Antiviral activities of plants occurring in the state of Minas Gerais, Brazil: Part 2. Screening Bignoniaceae species
Brando, Geraldo Célio;Kroon, Erna G.;Santos, Jo?o Rodrigues dos;Stehmann, Jo?o Renato;Lombardi,lio A.;Oliveira, Alaíde Braga de;
Revista Brasileira de Farmacognosia , 2010, DOI: 10.1590/S0102-695X2010005000035
Abstract: ethanol extracts of eighteen bignoniaceae species have been evaluated by the mtt assay for cytotoxicity in vero cells and for antiviral activity against human herpes virus type 1, vaccinia virus and murine encephalomyocarditis virus. among such species, seven are reported to be of traditional medicinal use no cytotoxicity was observed for most of the extracts up to the concentration of 500 μg/ml. fourteen (50%) of the 28 extracts assayed have disclosed antiviral activity with ec50 values in the range of 4.6+0.3 to 377.2+17.7 μg/ml. only two species, arrabidaea samydoides and callichlamys latifolia, have shown activity against all the three viruses. the extracts were chemically characterized by their tlc and hplc-dad profiles. mangiferin is the major constituent of a. samydoides but the isolated compound has been less active than the crude extract. this is the first report on the antiviral evaluation of the eighteen bignoniaceae species assayed.
Chemistry and Antiviral Activity of Arrabidaea pulchra (Bignoniaceae)
Geraldo Célio Brando,Erna G. Kroon,Danielle E.R. Souza,José D. Souza Filho,Alaíde Braga Oliveira
Molecules , 2013, DOI: 10.3390/molecules18089919
Abstract: The aim of the present work was to carry out a bioguided isolation of antiviral chemical constituents from an ethanol extract of leaves from Arrabidaea pulchra (Cham.) Sandwith (EEAPL) that had shown in vitro activity in a previous screening using DNA and RNA viruses. The activity of EEPAL was evaluated against the DNA viruses Human herpesvirus 1 (HSV-1) and Vaccinia virus Western Reserve (VACV-WR) as well as against the RNA viruses Murine encephalomyocarditis virus (EMCV), and Dengue virus 2 (DENV-2) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Cytotoxicity was determined in LLCMK 2 and Vero cells and the Selectivity Indexes (SI) were calculated. The most potent effect was observed against DENV-2 (EC 50 46.8 ± 1.6 μg mL ?1; SI 2.7). For HSV-1 and VACV-WR EC 50 values > 200 μg mL ?1 were determined, while no inhibition of the cytopathic effect was observed with EMCV. Bioguided fractionation of EEAPL by partition between immiscible solvents followed by chromatography over a Sephadex LH20 column afforded two arylpropanoid glycosides, verbascoside ( AP 1) and caffeoylcalleryanin ( AP 2), along with a terpenoid, ursolic acid ( AP 3). AP 1 and AP 3 exhibited similar anti-DENV-2 profiles, with SI values of 3.8 and 3.1, respectively, while AP 2 was the most effective anti-DENV-2 constituent, with a SI of 20.0. Our results show that A. pulchra leaves ethanol extract (EEAPL) affords compounds with antiviral activity, mainly against DENV-2.
Cytotoxicity of Wedelia paludosa D.C. extracts and constituents
Batista, Ronan;Brando, Geraldo C.;Braga, Fern?o C.;Oliveira, Alaíde B.;
Revista Brasileira de Farmacognosia , 2009, DOI: 10.1590/S0102-695X2009000100009
Abstract: wedelia paludosa d.c. (asteraceae) is an ornamental species occurring in many regions of brazil. aiming to find new cytotoxic compounds, the hydromethanol extract of w. paludosa (hme), as well as the dichloromethane (df) and water (wf) fractions resulting from its partition, were submitted to the brine shrimp lethality bioassay (bslb) in order to evaluate their cytotoxicity. dichloromethane fraction (df) was shown to be the most cytotoxic fraction (lc50 = 140.6 μg/ml), and its analysis by reversed phase high performance liquid chromatography (rp-hplc) revealed ent-kaurenoic (1, 6.22 ± 0.23%) and grandiflorenic (2, 3.22 ± 0.31%) acids as important constituents. hme (lc50 = 980 μg/ml), df (lc50 = 140.6 μg/ml), 1 (lc50 = 15.9 μg/ml) and 2 (lc50 = 29.8 μg/ml) were found to be cytotoxic, while the water fraction (wf, lc50 >> 1000 μg/ml) was inactive. as conclusion, the cytotoxicity observed for hme and df is mainly due to the presence of 1 and 2 in their constitution.
Análise da evolu??o tardia de 291 pacientes submetidos a substitui??o valvar por próteses metálicas
Brando, Carlos M. A;Pomerantzeff, Pablo M. A;Brando, Luiz C. A;Grinberg, Max;Stolf, Noedir A. G;Verginelli, Geraldo;Jatene, Adib D;
Revista Brasileira de Cirurgia Cardiovascular , 1995, DOI: 10.1590/S0102-76381995000100007
Abstract: between january 1980 and december 1993, 291 patients underwent valve replacement with mechanical valves in the heart institute of hcfmusp. one hundred and eighty seven (64.3%) patients were male, aged between 2 months and 78 years (average 38.3 +/-18.5). the ethiology was rheumatic in 132 patients (45.4%). were inserted 315 mechanical valves, 201 aortic, 77 mitral, 15 double aortic-mitral, 2 tricuspid, 1 double mitral-tricuspid, and 1 triple mitral-aortic-tricuspid. there were associated procedures in 164 patients (56.4%), the most frequent was replacement of ascending aorta in 49 (16.8%). one hundred and forty one patients (48.4%) underwent previous heart valve surgery. follow-up data are reported concerning functional status (nyha) and valve related complications. early mortality rate was 12.4% (36 patients). followup totaled 10078 months/patient (159 patients), with a mean follow-up period of 40.6 months. the linearized rates of thromboembolism, hemorrhage related to antithrombotic therapy, late death, endocarditis, paravalvular leak and hemolysis were 1.33%, 0.95%, 1.9%, 0.19%, 0.57% and 0.57% patients/year, respectively. fourteen year survival rate was 63.8%. eighty two percent of the patients were in functional class i in the postoperative period. it was possible to conclude that the patients submitted to mechanical valve replacement had a satisfactory clinical evolution.
Alteridade radical: implica??es para o cuidado em saúde
Carvalho, Liliane Brando;Freire, José Célio;Bosi, Maria Lúcia Magalh?es;
Physis: Revista de Saúde Coletiva , 2009, DOI: 10.1590/S0103-73312009000300017
Abstract: this paper intends to discuss ethics and care, its mutual relation and the implications of these concepts in the praxis developed in the public health services. so the authors adopted emmanuel lévinas's contributions as central reference in this essay. this discussion seems useful for a broader and deeper understanding of this practice, to (re)construct it from an ethical-political perspective, based on the dimension of the ethical listening of the care for the other: in this case, the user of health services.
A prática do psicólogo em saúde coletiva: um estudo no município de Fortaleza (CE), Brasil
Carvalho, Liliane Brando;Bosi, Maria Lúcia M.;Freire, José Célio;
Psicologia: Ciência e Profiss?o , 2009, DOI: 10.1590/S1414-98932009000100006
Abstract: the psychologist in the public health sector deals with the challenge of fitting in non-psychiatric institutions characterized as one specific locus of professional practice of health. this study intends to understand the meanings attributed by the psychologists to their practice developed in the public health institutions in fortaleza. based on qualitative approach from a critical-interpretative perspective, the sample was composed by eight psychologists who are part of the public health team. the main technique employed was the non-directive individual interview that allowed to construct two different axis related to the objectives. the results point out many difficulties experienced by the informants that vary from a precarious academic graduation until the influence of the biomedical model in the assistance organization. in the services explored, the psychologist assumes attitudes of care as technique, adjustment, control and nullification of the difference. there are, however, experiences that go beyond the traditional models of education and practice.
Dimens?o ética do cuidado em saúde mental na rede pública de servi?os
Carvalho,Liliane Brando; Bosi,Maria Lúcia Magalh?es; Freire,José Célio;
Revista de Saúde Pública , 2008, DOI: 10.1590/S0034-89102008005000033
Abstract: objective: to analyze the meanings of caring for people who seek assistance in mental health, from the perspective of psychologists engaged in their daily activities within public health services. methodological procedures: an exploratory qualitative study was conducted in the city of fortaleza, northeastern brazil, in 2006. the sample was composed of eight female informants, all of which were psychologists working in the state public health network. data was obtained by means of non-directive interviews which were taped and transcribed. categories were obtained from the discourses based on a hermeneutical approach by means of which an interpretive network was constructed. analisys of results: the interpretative network indicated that psychologists recognize their insertion in the field of public health as distinct from the professional field in which they obtained their training and, consequently, as a challenge. the predominant conceptions of care were circumscribed to the technical dimension, although other dimensions, closer to ethical concerns and to those related to respect for the "other" were also identified. conclusions: in the daily routine of assistance within the public health network, care is perceived as a technical attitude. it involves control and nullification of alterity, being more closely aligned to the traditional models of biomedicine and clinical psychology. however, other practices were observed that overcome this attitude. these emerging practices assume a new configuration, oriented towards affection, dialogue between professionals and those seeking assistance and an ethical commitment forged within a political and socio-cultural perspective.
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