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Search Results: 1 - 10 of 42 matches for " Gastrin "
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Assessing GastroPanel serum markers as a non-invasive method for the diagnosis of atrophic gastritis and Helicobacter pylori infection  [PDF]
Dominique Noah Noah, Marie Claire Okomo Assoumou, Servais Albert Fiacre Eloumou Bagnaka, Guy Pascal Ngaba, Ivo Ebule Alonge, Lea Paloheimo, Oudou Njoya
Open Journal of Gastroenterology (OJGas) , 2012, DOI: 10.4236/ojgas.2012.23024
Abstract: Gastric colonization by Helicobacter pylori increases the risk of gastric disorders, including atrophic gastritis which can be diagnosed based on levels of serum biomarkers like Gastrin and Pepsinogen. We therefore examined the efficacy of a serological-based method namely GastroPanel Blood kit, in diagnosing and scoring gastritis associated to Helicobacter pylori infection. Patients with dyspeptic symptoms were prospectively recruited on voluntary basis at the Yaounde Central Hospital and University Teaching Hospital, from March to July 2011. The degree of atrophy was classified according to levels in patient serum of pepsinogens I and II (PGI and PGII) and Gastrin 17 (G17) and compared with histological profiles as reference method. A specific ELISA test was used for the detection of H. pylori IgG antibodies. In total, 86 volunteers from 21 to 83 years old (mean = 46.4 ± 3.3) were enrolled, including 74.4% of women and 25.6% of men. The prevalence of gastritis was statistically similar between Gastro Blood Panel test and histology used as reference method (89.5% versus 83.7%: p > 0.20). Diagnosis based on serum makers showed high sensitivity (93.1%) in comparison with the reference method. However, the serological based method has diagnosed more atrophic gastritis than the reference (17.4% versus 7.0%: p < 0.01), especially at antrum of stomach with H. pylori infection. The prevalence of H. pylori infection was 81.4% with histology versus 84.9% with serology (GBP) (p > 0.05). Furthermore, the prevalence of H. pylori infection did not differ significantly between serological method (84.9%) and reference method (81.4%). These results suggest that diagnosis of atrophic gastritis and H. pylori infection obtained with an optional serological method (GastroPanel) is in a strong agreement with the biopsy findings, and thus can be a useful non endoscopic assessment of stomach mucosal atrophy in patients with dyspepsia.
Total gastrectomy with substitution of stomach by jejunal pouch with and without duodenal passage: study in rats
Aires Neto, Tertuliano;Cavalcante, Jeancarlo Fernandes;Brand?o-Neto, José;Araújo Filho, Irami;Almeida, Maria das Gra?as;Rezende, Adriana Augusto de;Egito, Eryvaldo Sócrates Tabosa;Azevedo, ítalo Medeiros de;Pinheiro, Laísa Araújo Mohana;Medeiros, Vítor Brasil;Medeiros, Aldo da Cunha;
Acta Cirurgica Brasileira , 2005, DOI: 10.1590/S0102-86502005000700019
Abstract: purpose: a comparison was done between the f. paulino jejunal pouch (fp) and a jejunal pouch (jp) as esophagus-duodenum interpositional graft, for replacing the stomach after total gastrectomy. it was investigated the effect of the two procedures on esophagus histology, nutritional state and serum gastrin in rats. methods: male wistar rats weighing 282±17g were randomly submitted to sham operation (s), fp and jp after total gastrectomy. after eight weeks the rats were killed with overdose of anesthetic and tissue was taken from the distal esophagus for histology. serum levels of total proteins, albumin, iron, transferring, folate, cobalamine, calcium, as well as serum gastrin were determined. survival was considered. results: fourty six rats were operated and thirty survived for eight weeks. five (33.3%) died after fp and 11 (52.3%) after jp (p<0.05). postoperative esophagitis occurred in 6 jp rats. at 8th week, no difference was observed on body weight when compared fp and jp rats (p>0.05). the jp rats had a significant decrease in serum albumin, glucose, transferrin, iron, folate and calcium, compared to sham (p<0.05). serum gastrin, iron and calcium were significantly higher in jp rats than in fp rats (p<0.05). in fp rats, transferrin and cobalamine showed significant decrease comparing the preoperative with 8th week levels (p<0.05). conclusion: f. paulino pouch in rats had lower mortality than jp, and esophagitis was not detected in it. jp rats had serum gastrin, iron and calcium unaffected, possibly because of preservation of duodenal passage.
Tumores neuroendócrinos gástricos e duodenal simultaneos
Saiote,Joana; Ramos,Gon?alo; Santos,Liliana; Dias,António Mateus; Bentes,Teresa; Barreiras,Jo?o;
Jornal Português de Gastrenterologia , 2012,
Abstract: gastrointestinal neuroendocrine tumors are relatively rare neoplasms and usually occur singly. the probability of simultaneous occurrence of gastric and duodenal carcinoid tumors is low. the authors report a case of a 73-year old male patient, diagnosed pernicious anemia 13 years ago, who underwent upper endoscopy for surveillance. the examination revealed atrophic gastritis and a polypoid lesion in the duodenal bulb, which was completely excised. pathological examination revealed gastric microcarcinoids in the context of atrophic gastritis and a neuroendocrine tumor in the duodenal bulb. the authors discuss the simultaneous occurrence of gastric carcinoid tumors and duodenal, in the context of pernicious anemia, addressing the etiopathogenic mechanism, treatment strategy and prognosis of these diseases
Interaction between substance P and gastrin-releasing peptide on thyrotropin secretion by rat pituitary in vitro
Moura, E.G.;Santos, C.V.M.;Santos, R.M.M.;Pazos-Moura, C.C.;
Brazilian Journal of Medical and Biological Research , 1999, DOI: 10.1590/S0100-879X1999000900015
Abstract: the effect of substance p (sp) on thyrotropin (tsh) secretion is controversial. in this study we evaluated the effect of sp on tsh secretion by hemipituitaries of 3-month-old wistar rats in vitro and its interaction with gastrin-releasing peptide (grp) at equimolar concentrations (1 μm and 10 μm). tsh release was measured under basal conditions and 30 min after incubation in the absence or presence of sp, grp or both peptides. pituitary tsh content was also measured in the pituitary homogenate after incubation. sp at both concentrations caused a significant (p<0.05) increase in tsh secretion compared with all other groups, which was approximately 60% (1 μm) and 85% (10 μm) higher than that of the control group (23.3 ± 3.0 ng/ml). grp at the lower concentration did not produce a statistically significant change in tsh secretion, whereas at the concentration of 10 μm it produced a 50% reduction in tsh. grp co-incubated with substance p completely blocked the stimulatory effect of sp at both concentrations. pituitary tsh content decreased in the sp-treated group compared to controls (0.75 ± 0.03 μg/hemipituitary) at the same proportion as the increase in tsh secretion, and this effect was also blocked when grp and sp were co-incubated. in conclusion, in an in vitro system, sp increased tsh secretion acting directly at the pituitary level and this effect was blocked by grp, suggesting that grp is more potent than sp on tsh secretion, and that this inhibitory effect could be the predominant effect in vivo.
Role of annexin II in pancreatic and colon cancer cell growth promotion
Agata Ptak-Belowska,Aneta Targosz,Gracjana Krzysiek-M?czka,Tomasz Brzozowski
Polish Gastroenterology , 2010,
Abstract: Introduction: Gastrin exerts growth stimulatory and co-carcinogenic effects in gastrointestinal (GI) tract. Gastrin peptide mediates its growth effects by binding to CCK1 and CCK2 receptors. However, recently annexin II (ANX II) has been identified as a high affinity binding protein for gastrin and its precursors. It's known that ANX II binds gastrin and mediates some growth factor effects on GI cancer cells. Aim of the study: We assessed the role and possible interactions between ANX II and gastrin in growth of colon and pancreatic cancer cells. Human pancreatic and colon cancer cell lines in in vitro models were investigated. Material and methods: The human pancreatic, colon cancer cell lines were used. Cancer cell lines with or without treatment with gastrin were investigated in terms of protein level of ANX II by Western Blot method. Growth effects exerted by hormone gastrin were assessed using MTT assay method. Results and conclusions: Gastrin peptide promotes colon and pancreatic cancer cells growth and leads to an increase in ANX II protein expression in these cells.
Ghrelin and gastrin in advanced gastric cancer before and after gastrectomy
Anna Zub-Pokrowiecka, Kazimierz Rembiasz, Peter C Konturek, Andrzej Budzyński, Stanis?aw J Konturek, Marek Winiarski, W?adys?aw Bielański
World Journal of Gastroenterology , 2011,
Abstract: AIM: To investigate plasma ghrelin, gastrin and growth hormone secretagogue receptor (GHS-R) expression in advanced gastric cancer (GC) before and after resection.METHODS: Seventy subjects in whom endoscopy of the upper gastrointestinal tract was performed in the Department of General Surgery at Cracow University during the past decade: (1) 25 patients with GC associated with Helicobacter pylori (H. pylori) infection; (2) 10 patients with GC 4-5 years after (total or subtotal) gastrectomy; (3) 25 healthy H. pylori-negative controls, matched by age and BMI to the above two groups; and (4) 10 GC patients 4-5 years after total gastrectomy. Ghrelin and gastrin plasma concentrations were measured by specific radioimmunoassay under fasting conditions and postprandially at 60 and 90 min after ingestion of a mixed meal. GHS-R expression was examined in biopsy samples from intact healthy mucosa and GC tissue using semi-quantitative reverse transcription-polymerase chain reaction.RESULTS: In healthy controls, fasting plasma ghrelin levels were significantly elevated and declined markedly at 60 and 90 min after a mixed meal. The concomitant enhanced ghrelin, GHS-R and gastrin expression in GC tissue over that recorded in intact mucosa, and the marked rise in plasma gastrin in these subjects under fasting conditions indicate the role of these hormonal factors in GC formation. Fasting plasma levels and postprandial response of ghrelin and gastrin appear to be inversely correlated in healthy subjects. Feeding in the controls resulted in a significant fall in plasma ghrelin with a subsequent rise in plasma gastrin, but in H. pylori-positive GC patients submitted to total or distal gastrectomy, feeding failed to affect significantly the fall in plasma ghrelin that was recorded in these patients before surgery. Fasting ghrelin concentrations were significantly lower in patients 4-5 years after total gastrectomy compared to those in healthy controls and to these in GC patients before surgery.CONCLUSION: Elevated plasma gastrin and suppression of fasting ghrelin in patients with GC suggest the existence of a close relationship between these two hormones in gastric carcinogenesis.
Gastric carcinoid in a patient infected with Helicobacter pylori: A new entity?
Pantelis Antonodimitrakis,Apostolos Tsolakis,Staffan Welin,Gordana Kozlovacki
World Journal of Gastroenterology , 2011, DOI: 10.3748/wjg.v17.i25.3066
Abstract: There are four types of gastric carcinoid tumors, classified according to their histology and malignant potential. Only a few cases of carcinoid tumors in patients infected with Helicobacter pylori (H. pylori) have been reported so far. We report a patient infected with H. pylori presenting with a small solitary gastric carcinoid tumor with very low proliferative rate and normal gastrin levels. The tumor was endoscopically removed and the patient received an eradication therapy against H. pylori. No signs of metastatic disease have been found so far during more than 3 year of follow-up. Infection with H. pylori may cause chronic gastritis with normal or elevated gastrin levels, leading to the development of gastric carcinoids by mechanisms unrelated to gastrin. Enterochromaffin-like cell tumors related to a chronic H. pylori infection may be considered as a distinct type of gastric carcinoid tumors.
Interaction between substance P and gastrin-releasing peptide on thyrotropin secretion by rat pituitary in vitro
Moura E.G.,Santos C.V.M.,Santos R.M.M.,Pazos-Moura C.C.
Brazilian Journal of Medical and Biological Research , 1999,
Abstract: The effect of substance P (SP) on thyrotropin (TSH) secretion is controversial. In this study we evaluated the effect of SP on TSH secretion by hemipituitaries of 3-month-old Wistar rats in vitro and its interaction with gastrin-releasing peptide (GRP) at equimolar concentrations (1 μM and 10 μM). TSH release was measured under basal conditions and 30 min after incubation in the absence or presence of SP, GRP or both peptides. Pituitary TSH content was also measured in the pituitary homogenate after incubation. SP at both concentrations caused a significant (P<0.05) increase in TSH secretion compared with all other groups, which was approximately 60% (1 μM) and 85% (10 μM) higher than that of the control group (23.3 ± 3.0 ng/ml). GRP at the lower concentration did not produce a statistically significant change in TSH secretion, whereas at the concentration of 10 μM it produced a 50% reduction in TSH. GRP co-incubated with substance P completely blocked the stimulatory effect of SP at both concentrations. Pituitary TSH content decreased in the SP-treated group compared to controls (0.75 ± 0.03 μg/hemipituitary) at the same proportion as the increase in TSH secretion, and this effect was also blocked when GRP and SP were co-incubated. In conclusion, in an in vitro system, SP increased TSH secretion acting directly at the pituitary level and this effect was blocked by GRP, suggesting that GRP is more potent than SP on TSH secretion, and that this inhibitory effect could be the predominant effect in vivo.
Role of p38 Signaling Pathway in Pentagastrin-Regulated Cell Proliferation of Colorectal Carcinoma Cell Line HT-29  [PDF]
Jiading Mao, Pei Wu, Jian Wu, Liang Tao, Ping Wu, Guang Yang, Wenwen Guo, Jun Wang
Journal of Cancer Therapy (JCT) , 2016, DOI: 10.4236/jct.2016.76041
Abstract: Objective: To investigate the effects and mechanisms of p38 signaling pathway in pentagastrin-regulated cell proliferation of colorectal carcinoma cell line HT-29. Methods: HT-29 cell line of colorectal carcinoma was in vitro incubated and divided into the control group, pentagastrin group, proglumide group, and pentagastrin + proglumide group. MTT reduction assay was performed to detect the proliferation status of HT-29 cell line and determine the optimal dosage of pentagastrin and proglumide. Annexin V-fluorescein isothiocyanate flow cytometry was used to detect the proliferation index (PI) and apoptosis rate (AR) of HT-29 cells. Reverse transcriptase polymerase chain reaction was performed to detect the mRNA expression of the pentagastrin receptor/cholecystokinin-B receptor (CCK-BR) and p38. The protein and phosphorylation levels of p38 were estimated by western blotting. Results: RT-PCR detection showed that CCK-BR mRNA was expressed in the HT-29 cell line. Pentagatrin improved HT-29 cell proliferation in dosage of 6.25 - 100 mg/L, and the optimal dosage of pentagastrin was 25.0 mg/L. Proglumide had no significant effect on the proliferation of HT-29 cells, but significantly inhibited the proliferation of HT-29 cells stimulated by pentagastrin when the dosage of proglumide was 8.0 - 128.0 mg/L, and the optimal dosage was 32.0 mg/L. The AR in the pentagastrin group was significantly lower than that in the control group and in the pentagastrin + proglumide group. The PI in the pentagastrin group was significantly higher than that in the control group and in the pentagastrin + proglumide group. P38 phosphorylation level in the pentagastrin group was significantly lower than that in the control group, and in the pentagastrin + proglumide group. There were no significant differences in the mRNA and protein expression of p38 in the control, pentagastrin, proglumide and pentagastrin + proglumide groups. Conclusion: Pentagastrin can improve proliferation of the CRC cell line HT-29 and inhibit apoptosis via the p38 signal transduction pathway. This mechanism may be associated with suppressed p38 protein phosphorylation level due to inhibition of proglumide, a gastrin receptor antagonist.
Diagnosis of Zollinger-Ellison syndrome: Increasingly difficult
Tetsuhide Ito,Guillaume Cadiot,Robert T Jensen
World Journal of Gastroenterology , 2012, DOI: 10.3748/wjg.v18.i39.5495
Abstract: In the present paper the increasing difficulty of diagnosis of Zollinger-Ellison syndrome (ZES) due to issues raised in two recent papers is discussed. These issues involve the difficulty and need to withdraw patients suspected of ZES from treatment with Proton Pump Inhibitors (omeprazole, esomeprazole, lansoprazole, rabeprazole, pantoprazole) and the unreliability of many gastrin radioimmunoassays. The clinical context of each of these important issues is reviewed and the conclusions in these articles commented from the perspective of clinical management.
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