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Search Results: 1 - 10 of 189745 matches for " G.;Sandrini "
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Adrenocortical tumors in children
Ribeiro, R.C.;Michalkiewicz, E.L.;Figueiredo, B.C.;DeLacerda, L.;Sandrini, F.;Pianovsky, M.D.;Sampaio, G.;Sandrini, R.;
Brazilian Journal of Medical and Biological Research , 2000, DOI: 10.1590/S0100-879X2000001000013
Abstract: childhood adrenocortical tumors (act) are rare. in the usa, only about 25 new cases occur each year. in southern brazil, however, approximately 10 times that many cases are diagnosed each year. most cases occur in the contiguous states of s?o paulo and paraná. the cause of this higher rate has not been identified. familial genetic predisposition to cancer (p53 mutations) and selected genetic syndromes (beckwith-wiedemann syndrome) have been associated with childhood act in general but not with the brazilian counterpart. most of the affected children are young girls with classic endocrine syndromes (virilizing and/or cushing). levels of urinary 17-ketosteroids and plasma dehydroepiandrosterone sulfate (dhea-s), which are abnormal in approximately 90% of the cases, provide the pivotal clue to a diagnosis of act. typical imaging findings of pediatric act consist of a large, well-defined suprarenal tumor containing calcifications with a thin capsule and central necrosis or hemorrhage. the pathologic classification of pediatric act is troublesome. even an experienced pathologist can find it difficult to differentiate carcinoma from adenoma. surgery is the single most important procedure in the successful treatment of act. the role of chemotherapy in the management of childhood act has not been established although occasional tumors are responsive to mitotane or cisplatin-containing regimens. because of the heterogeneity and rarity of the disease, prognostic factors have been difficult to establish in pediatric act. patients with incomplete tumor resection or with metastatic disease at diagnosis have a dismal prognosis. in patients with localized and completely resected tumors, the size of the tumor has predictive value. patients with large tumors have a much higher relapse rate than those with small tumors.
Clinical and molecular genetics of primary pigmented nodular adrenocortical disease
Sandrini, Fabiano;Stratakis, Constantine;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2004, DOI: 10.1590/S0004-27302004000500007
Abstract: carney complex (cnc) is a multiple endocrine neoplasia (men) syndrome associated with other, non-endocrine manifestations such as lentigines, cardiac myxomas and schwannomas. primary pigmented nodular adrenocortical disease (ppnad), leading to corticotrophin-independent cushing's syndrome is the most frequent endocrine lesion in cnc. the complex has been mapped to 2p16 and 17q22-24, although additional heterogeneity may exist. the gene coding for the protein kinase a (pka) type i-a regulatory subunit (ria), prkar1a, had been mapped to 17q. cloning of the prkar1a genomic structure and its sequencing showed mutations in cnc-, cnc with ppnad- and sporadic ppnad-patients. in cnc tumors, pka activity showed increased stimulation by camp, whereas pka activity ratio was decreased, and in cnc tumors, there is loh of the normal allele, suggesting that normal prkar1a may be a tumor suppressor in these tissues. cnc is the first human disease caused by mutations of one of the subunits of the pka enzyme, a critical component of the camp signaling system and a potential participant in many other signaling pathways.
ELISA for Determination of Human Growth Hormone: Recognition of Helix 4 Epitopes
Juliana F. Moura,Luiz DeLacerda,Romolo Sandrini,Fernanda M. Borba,Denise N. Castelo,Elis R. Sade,Sandra Sella,Jo o C. Minozzo,Luis G. Callefe,Bonald C. Figueiredo
Journal of Biomedicine and Biotechnology , 2004, DOI: 10.1155/s1110724304308090
Abstract: Human growth hormone (hGH) signal transduction initiates with a receptor dimerization in which one molecule binds to the receptor through sites 1 and 2. A sandwich enzyme-linked immunosorbent assay was developed for quantifying hGH molecules that present helix 4 from binding site 1. For this, horse anti-rhGH antibodies were eluted by an immunoaffinity column constituted by sepharose-rhGH. These antibodies were purified through a second column with synthetic peptide correspondent to hGH helix 4, immobilized to sepharose, and used as capture antibodies. Those that did not recognize synthetic peptide were used as a marker antibody. The working range was of 1.95 to 31.25 ng/mL of hGH. The intra-assay coefficient of variation (CV) was between 4.53% and 6.33%, while the interassay CV was between 6.00% and 8.27%. The recovery range was between 96.0% to 103.8%. There was no cross-reactivity with human prolactin. These features show that our assay is an efficient method for the determination of hGH.
Amplification of 9q34 in childhood adrenocortical tumors: a specific feature unrelated to ethnic origin or living conditions
Figueiredo, B.C.;Ribeiro, R.C.;Zambetti, G.;Haddad, B.;Pianovsky, M.D.;Pereira, R.M.;DeLacerda, L.;Sandrini, R.;
Brazilian Journal of Medical and Biological Research , 2000, DOI: 10.1590/S0100-879X2000001000012
Abstract: adrenocortical tumors (act) in children under 15 years of age exhibit some clinical and biological features distinct from act in adults. cell proliferation, hypertrophy and cell death in adrenal cortex during the last months of gestation and the immediate postnatal period seem to be critical for the origin of act in children. studies with large numbers of patients with childhood act have indicated a median age at diagnosis of about 4 years. in our institution, the median age was 3 years and 5 months, while the median age for first signs and symptoms was 2 years and 5 months (n = 72). using the comparative genomic hybridization technique, we have reported a high frequency of 9q34 amplification in adenomas and carcinomas. this finding has been confirmed more recently by investigators in england. the lower socioeconomic status, the distinctive ethnic groups and all the regional differences in southern brazil in relation to patients in england indicate that these differences are not important to determine 9q34 amplification. candidate amplified genes mapped to this locus are currently being investigated and southern blot results obtained so far have discarded amplification of the abl oncogene. amplification of 9q34 has not been found to be related to tumor size, staging, or malignant histopathological features, nor does it seem to be responsible for the higher incidence of act observed in southern brazil, but could be related to an act from embryonic origin.
The pedagogy and educational praxis of Jo o Bosco
Renato Kraide Soffner; P. Marcos Sandrini, SDB
Revista de Ciências da Educa??o , 2010,
Abstract: This paper presents the pedagogical model developed by the Italian priest Jo o Bosco (1815-1888), and the associated praxis to such a model, from the point of view of a historical analysis that projects the life and formation context of the character. Such a model, called the Preventive System, was the foundation of an educational action that came to our days through the continuity of the work of Bosco, and that, in accordance with the conclusions of this work, can certainly be applied to contemporary educational processes. The affection and reason may be associated in the pedagogical actions, showing an interesting convergence to be exploited by the educators of our days.
Childhood Adrenocortical Tumours: a Review
Rosana Marques-Pereira, Luiz DeLacerda, Hadriano M Lacerda, Edson Michalkiewicz, Fabiano Sandrini, Romolo Sandrini
Hereditary Cancer in Clinical Practice , 2006, DOI: 10.1186/1897-4287-4-2-81
Abstract: Adrenocortical tumours (ACT) occur at all ages and have a bimodal distribution with peaks of incidence at around 5 years of age and in the 4th and 5th decades of life [1,2]. Childhood ACTs are rare, with the annual worldwide incidence around 0.34 per million children below the age of 15 [1,3]. In the United States, Surveillance Epidemiology and End Results (SEER) data from the National Cancer Institute show that only about 0.2% of all childhood malignancies are ACTs [4].In southern Brazil (State of Paraná), the ACT incidence is 4.2 per million in the age range of 0 - 15 years [5] with higher prevalence in 0 - 4-year-old girls (Fig. 1). Over the last 40 years, more than 137 ACT patients under 19 years of age have been admitted for treatment in the Pediatric Endocrinology Unit of the Department of Pediatrics in the Clinical Hospital of the Federal University of Paraná, in Curitiba, Brazil. This hospital is a tertiary referral centre for a population of approximately 16 million people, 5 million of whom are less than 15 years of age. It should be noted that at least 5 other hospitals in our state are referral centres for childhood malignancies. In the neighbouring State of S?o Paulo, Marigo et al. observed that childhood ACTs accounted for 2.9% of solid tumours registered at Santa Casa de Misericordia of S?o Paulo over a period of 15 years, whereas in the London Hospital for Sick Children, ACTs comprised only 0.8% [6]. Later on, other groups in the State of S?o Paulo also reported a large series of ACTs in children and adolescents [7-10].The population in these two Brazilian states are descendants of migrants from Portugal, Italy, Spain, Germany, Poland, Ukraine, Japan and Africa - countries where the incidence of ACTs is not increased - and a minority of the native population. The sporadic occurrence, absence of any clinical characteristics consistent with genetic disorders (i.e. Beckwith-Wiedemann and MEN1 syndromes, hemihypertrophy, congenital adrenal hyperplasia an
Integration in Working Memory: A Magnetic Stimulation Study on the Role of Left Anterior Prefrontal Cortex
Nicola De Pisapia,Marco Sandrini,Todd S. Braver,Luigi Cattaneo
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0043731
Abstract: Integration is a fundamental working memory operation, requiring the insertion of information from one task into the execution of another concurrent task. Previous neuroimaging studies have suggested the involvement of left anterior prefrontal cortex (L-aPFC) in relation to working memory integration demands, increasing during presentation of information to be integrated (loading), throughout its maintenance during a secondary task, up to the integration step, and then decreasing afterward (unloading). Here we used short bursts of 5 Hz repetitive Transcranic Magnetic Stimulation (rTMS) to modulate L-aPFC activity and to assess its causal role in integration. During experimental blocks, rTMS was applied (N = 10) over L-aPFC or vertex (control site) at different time-points of a task involving integration of a preloaded digit into a sequence of arithmetical steps, and contrasted with a closely matched task without integration demand (segregation). When rTMS was applied during the loading phase, reaction times during secondary task were faster, without significant changes in error rates. RTMS instead worsened performance when applied during information unloading. In contrast, no effects were observed when rTMS was applied during the other phases of integration, or during the segregation condition. These results confirm the hypothesis that L-aPFC is causally and selectively involved in the integration of information in working memory. They additionally suggest that pre-integration loading and post-integration unloading of information involving this area may be active and resource-consuming processes.
Un nuevo método de análisis digital para la determinación de los niveles de consumo foliar
Sandrini-Neto,Leonardo; Hostin,Leonardo; Lana,Paulo; Pellizzari,Franciane;
Investigaciones marinas , 2007, DOI: 10.4067/S0717-71782007000100011
Abstract: a protocol using the computer program adobe photoshop cs2 (version 9.0) is proposed for determining the level of herbivory on intact and consumed leaves, using mangroves as an example. the usual manual methods consist of drawing the leaf on graph paper, restoring the original shape in cases of damaged edges. the computerized methods are intended to accelerate the leaf measurement time with applications, digitalization equipment, and portable area measurers that are more efficient, faster, and more economically viable than the manual methods. the need to restore the edges of the consumed leaves is an obstacle to all methodologies. nonetheless, the reconstruction of the consumed foliar edges is shown to be more realistic with adobe photoshop, as this allows a better fit to the original shape of the leaf. moreover, this application allows the use of compacted files such as jpeg, permitting a simple, rapid, and precise method for determining the levels or rates of foliar herbivory
Compreendendo a lacuna entre a prática e a evolu??o técnico-científica do banho no leito
Maciel, Silaine Sandrini Alves;Bocchi, Silvia Cristina Mangini;
Revista Latino-Americana de Enfermagem , 2006, DOI: 10.1590/S0104-11692006000200013
Abstract: the objectives of this qualitative study were to understand the experience of the nursing dependent person for the bed-bath and develop a theoretical model of this experience. the symbolic interactionism perspective and grounded theory methodology were used to develop the study. observation and interview were the strategies used for data collection. based on data analysis, a phenomenon was identified: learning to assess life and nursing when the patient becomes dependent on nursing care for the bed-bath. the theoretical model shows the meaning of the patient experience in the bed-bath, integrated by the core category - proposing a nursing model for the bed-bath according to the patient assessment process, as opposed to the technical-scientific evolution of this procedure.
Non-invasive genetic approaches for estimation of ungulate population size: a study on roe deer (Capreolus capreolus) based on faeces
Ebert, C.,Sandrini, J.,Spielberger, B.,Thiele, B.
Animal Biodiversity and Conservation , 2012,
Abstract: Estimating population size is particularly difficult for animal species living in concealing habitats with dense vegetation. This is the case for roe deer as for many other ungulates. Our objective was to develop a non–invasive genetic capture–mark–recapture approach based on roe deer faeces collected along transects. In a pilot study, we collected 1,790 roe deer faeces during five sampling days in a forested study area in south western Germany. We extracted DNA from 410 of these samples and carried out microsatellite analysis using seven dinucleotide markers. The analyses resulted in 328 useable consensus genotypes which were assigned to 174 individuals. The population size estimated using a Bayesian approach was 94 (82–111) male and 136 (121–156) female roe deer. Our study shows that non–invasive genetic methods are a valuable management tool for roe deer.
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