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Search Results: 1 - 10 of 189881 matches for " G.;Revelli "
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Coagulación de la leche. Desarrollo de un dispositivo para el “monitoreo” online del proceso. Avances en la Argentina
SBODIO, O.A.,REVELLI, G.R.2
RIA : Revista de Investigaciones Agropecuarias , 2012,
Abstract: La coagulación enzimática de la leche es una etapa fundamental en la elaboración de queso. Su controlonline tiene por objeto la determinación del tiempo de coagulación, la velocidad de crecimiento de la firmeza yel apropiado tiempo de corte de la cuajada. El tratamiento térmico de la leche permite incorporar al queso proteínasdel suero para incrementar su valor biológico y su rendimiento. También, numerosas investigacionesreportan el agregado de Proteínas de Suero en Polvo (WPC) en la elaboración de distintos tipos de quesos.Tratamientos diversos de las proteínas del suero son utilizados para alimentos ricos en almidones. El desarrollode un dispositivo que cumpla con los requerimientos de fortaleza, higiene en el lugar, que no impida lasoperaciones de corte y agitación y que no sea destructivo, será una herramienta importante en el estudio delos efectos de las variables críticas, el control de la elaboración de queso y yogurt, y en el desarrollo de nuevosproductos. Una revisión histórica muestra que desde hace varias décadas los investigadores se preocupan por obtener dispositivos basados en diferentes métodos. En la Argentina, se reportan experiencias con utilización del principio del alambre caliente. En la actualidad, se construye un dispositivo innovador que puede seraplicado en el desarrollo de nuevos productos y en tina quesera industrial.
Administration of interferon-g to pregnant rats reverses the depressed adjuvant-induced arthritis of their chronically Trypanosoma cruzi-infected offspring
Didoli G.,Revelli S.,Davila H.,Ferro M.E.
Brazilian Journal of Medical and Biological Research , 1999,
Abstract: We demonstrated that administration of interferon gamma (IFN-g) to the inbred "l" strain of pregnant rats conferred partial resistance on their offspring to challenge with Trypanosoma cruzi. We now examine if this intervention also modifies the reportedly immunodepressed cellular responses which occur during chronic infection. Offspring were born to mothers undergoing one of the following procedures during gestation: subcutaneous injections of recombinant rat IFN-g, 50,000 IU/rat, five times/week for 3 weeks, which was started on the day of mating (IFN-Mo); infection with 106 trypomastigotes of T. cruzi at 7, 14, and 21 days after mating plus IFN-g treatment as given to the former group (TcIFN-Mo); the same protocol except that physiological saline was injected instead of IFN-g (Tc-Mo); injection of physiological saline only (control-Mo). All offspring groups (N = 8-10/group) were infected at weaning and were assessed 90 days later for their adjuvant-induced arthritic response or levels of major T cell subsets in spleen and lymph nodes. TcIFN-Mo and IFN-Mo offspring showed a reestablished arthritic response, which remained within the range seen in controls. Immunolabeling studies on parallel groups of 90-day-infected offspring showed that the inverse CD4/CD8 cell ratio that is usually seen in lymphoid organs from these chronically infected rats (median 0.61) appeared to have recovered in the TcIFN-Mo and IFN-Mo groups (median 1.66 and 1.78, respectively) and was not different from uninfected controls (1.96). These studies indicate that early stimulation with IFN-g is able to reverse the immunosuppressive state that is usually present during the chronic period of the experimental infection.
Il catalogo (e il catalogatore): vaso di coccio tra vasi di ferro? The catalogue (and the cataloguer): a defenseless entity?
Carlo Revelli
JLIS.it , 2012, DOI: 10.4403/jlis.it-5621
Abstract: The author reflects upon the work of cataloguing in present times. He wonders how to harmonize the precision and universality of cataloguing rules and the growing of different cultural needs and information behaviours. Against the widespread ideas on “disintermediation” allowed by information and communication technologies, the author reaffirms the crucial role, although with a quite new background, of librarians and cataloguers as intermediaries between users and documents, between citizens and the information world. L'autore riflette sul lavoro di catalogazione, in particolar modo sulle modalità di armonizzare la precisione e l'universalità delle regole di catalogazione e il numero sempre crescente di bisogni culturali e informativi differenziati. In contrapposizione alle idee sempre più diffuse della disintermediazione portate avanti dalle tecnologie dell'informazione e della comunicazione, l'autore riafferma il ruolo cruciale dei bibliotecari e dei catalogatori come intermediari tra l'utente e i documenti, tra i cittadini e il mondo dell'informazione.
Propiedades de los Geles Lácteos formados por la Acción Combinada de Enzimas Coagulantes y Glucono-d-Lactona
Sbodio,O.A.; Tercero,E.J.; Coutaz,V.R.; Revelli,G.R.;
Información tecnológica , 2005, DOI: 10.4067/S0718-07642005000400004
Abstract: a centered rotational design was used to test the influence of temperature, ph and the quantity of rennet on combined rennet and glucono-δ-lactone whole milk gels. characteristic parameters of coagulation: tmax, dmax and tmaxv were measured by the hot wire method. different quantities of glucono-δ-lactone were added to reach ph 5.8 in one hour. when using calf rennet, the main effect was produced by enzyme concentration, followed by ph and temperature. using microbial protease the sequence of importance was enzyme concentration, temperature, and ph. from tmax and dmax models, it was concluded that enzyme concentration showed the highest positive influence. the maximum tmaxv voltages were significantly influenced by the temperature and the bovine enzyme concentration (p<0.01) and microbial protease (p>0.01). the lowest speed of voltage increase of dmax to tmax was obtained with bovine enzyme. whole milk coagulated with both coagulants reached the maximum voltage at the same time
Administration of interferon-g to pregnant rats reverses the depressed adjuvant-induced arthritis of their chronically Trypanosoma cruzi-infected offspring
Didoli, G.;Revelli, S.;Davila, H.;Ferro, M.E.;Romero-Piffiguer, M.;Bottasso, O.;
Brazilian Journal of Medical and Biological Research , 1999, DOI: 10.1590/S0100-879X1999000600011
Abstract: we demonstrated that administration of interferon gamma (ifn-g) to the inbred "l" strain of pregnant rats conferred partial resistance on their offspring to challenge with trypanosoma cruzi. we now examine if this intervention also modifies the reportedly immunodepressed cellular responses which occur during chronic infection. offspring were born to mothers undergoing one of the following procedures during gestation: subcutaneous injections of recombinant rat ifn-g, 50,000 iu/rat, five times/week for 3 weeks, which was started on the day of mating (ifn-mo); infection with 106 trypomastigotes of t. cruzi at 7, 14, and 21 days after mating plus ifn-g treatment as given to the former group (tcifn-mo); the same protocol except that physiological saline was injected instead of ifn-g (tc-mo); injection of physiological saline only (control-mo). all offspring groups (n = 8-10/group) were infected at weaning and were assessed 90 days later for their adjuvant-induced arthritic response or levels of major t cell subsets in spleen and lymph nodes. tcifn-mo and ifn-mo offspring showed a reestablished arthritic response, which remained within the range seen in controls. immunolabeling studies on parallel groups of 90-day-infected offspring showed that the inverse cd4/cd8 cell ratio that is usually seen in lymphoid organs from these chronically infected rats (median 0.61) appeared to have recovered in the tcifn-mo and ifn-mo groups (median 1.66 and 1.78, respectively) and was not different from uninfected controls (1.96). these studies indicate that early stimulation with ifn-g is able to reverse the immunosuppressive state that is usually present during the chronic period of the experimental infection.
Recuento de bacterias totales en leche cruda de tambos que caracterizan la zona noroeste de Santa Fe y sur de Santiago del Estero
Revelli,G.R.; Sbodio,O. A.; Tercero,E.J.;
Revista argentina de microbiolog?-a , 2004,
Abstract: a total of 6.998 raw milk samples of bulk tank, belonging to 55 dairy farms associated to the cooperativa tambera nueva alpina ltda., were collected between the years 1993 and 2002. the total mesophilic aerobic microorganisms count was analyzed, obtaining a medium value of 1.2 x 105± 2.4 x 105cfu/ml that characterizes the zone. the final year of experience, observed a 97% of dairy farms evaluated with averages £ 1,0 x 105cfu/ml. only a 3% it surpasses this limit, not being found establishments with levels over 1.5 x 105cfu/ml. relating this indicators with compositionals parameters, the most significant correlation resulted for: total bacterial count vs acidity (r = 0.292; p < 0.001) and total bacterial count vs freezing point (r = 0.157; p < 0.001. the microbiological quality in raw milk of dairy farms belonging to the northwest zone of santa fe and south of santiago del estero showed a significant improvement during this time.
Estudio y evolución de la calidad de leche cruda en tambos de la zona noroeste de Santa Fe y sur de Santiago del Estero, Argentina (1993–2009)
REVELLI, G.R.,SBODIO, O.A.,TERCERO, E.J.
RIA : Revista de Investigaciones Agropecuarias , 2011,
Abstract: Un total de 10.704 muestras de leche cruda de pool de tanque, correspondientes a 55 establecimientos lecheros asociados a la Cooperativa Tambera y Agropecuaria Nueva Alpina Ltda., fueron recolectadas entre los a os 1993 y 2009. En el marco del Programa de Mejoramiento Integral de Calidad de Leche, se analizaron parámetros físico-químicos, microbiológicos y sanitarios, obteniéndose valores medios que caracterizan la zona, a saber: acidez: 16,30 ± 0,96 °D, pH: 6,68 ± 0,04, grasa butirosa: 3,48 ± 0,24%, proteína verdadera:3,11 ± 0,12%, lactosa: 4,74 ± 0,16%, cenizas: 0,70 ± 0,09%, sólidos totales: 12,18 ± 0,42%, descenso crioscópico: -0,530 ± 0,02 °C, recuento de bacterias totales: 9,6 x 104 ± 2,2 x 105 UFC/ml, recuento de células somáticas: 407.000 ± 230.000 CS/ml e inhibidores (Antibióticos): 99,64% negativo. Las correlaciones más significativas resultaron para: grasa butirosa vs sólidos totales (r = 0,784; P < 0,001) y proteína verdadera vs sólidos totales (r = 0,557; P < 0,001). Durante 17 a os de experiencia se estudió la calidad composicional en leche cruda de tambos que pertenecen a la zona noroeste de Santa Fe y sur de Santiago del Estero, observándoseun significativo mejoramiento, especialmente en aquellos indicadores que infieren un alto valor industrial. Optimizar la capacidad de gestión de los productores y la calidad operativa de los tamberos, contribuyó en el logro de los resultados obtenidos.
Propiedades de los Geles Lácteos formados por la Acción Combinada de Enzimas Coagulantes y Glucono-d-Lactona Properties of Whole Milk Gels formed by a Combination of Rennet and Glucono-d-Lactone
O.A. Sbodio,E.J. Tercero,V.R. Coutaz,G.R. Revelli
Información Tecnológica , 2005,
Abstract: Se utilizó un dise o rotacional centrado para medir la influencia de la temperatura, el pH y la cantidad de enzima coagulante sobre geles formados por la acción combinada de enzimas coagulantes y glucono-δ-lactona. Las respuestas t max, Dmax y TmaxV fueron medidas por el método del alambre caliente. Para alcanzar pH 5.8 en una hora se utilizaron diferentes concentraciones de glucono-δ-lactona. Cuando se usó coagulante bovino el principal efecto, en orden de importancia, fue producido por la concentración enzimática, el pH y la temperatura. Cuando se usó proteasa microbiana este orden fue, concentración enzimática, temperatura y pH. A partir de los modelos obtenidos para t max y Dmax se puede concluir que la concentración enzimática es la variable que más influye positivamente. Los máximos voltajes TmaxV fueron influenciados significativamente por la temperatura y la concentración enzimática de origen bovino (p < 0.01) y proteasa microbiana (p > 0.01). La menor velocidad de incremento de voltaje Dmax a t max se obtuvo con enzima de origen bovino. La leche entera coagulada con ambos coagulantes alcanzan el máximo voltaje al mismo tiempo. A centered rotational design was used to test the influence of temperature, pH and the quantity of rennet on combined rennet and glucono-δ-lactone whole milk gels. Characteristic parameters of coagulation: t max, Dmax and TmaxV were measured by the hot wire method. Different quantities of glucono-δ-lactone were added to reach pH 5.8 in one hour. When using calf rennet, the main effect was produced by enzyme concentration, followed by pH and temperature. Using microbial protease the sequence of importance was enzyme concentration, temperature, and pH. From t max and Dmax models, it was concluded that enzyme concentration showed the highest positive influence. The maximum TmaxV voltages were significantly influenced by the temperature and the bovine enzyme concentration (p<0.01) and microbial protease (p>0.01). The lowest speed of voltage increase of Dmax to Tmax was obtained with bovine enzyme. Whole milk coagulated with both coagulants reached the maximum voltage at the same time
Recuento de bacterias totales en leche cruda de tambos que caracterizan la zona noroeste de Santa Fe y sur de Santiago del Estero Total bacterial count in raw milk of dairy farms that characterize the northwest zone of Santa Fe and south of Santiago del Estero
G.R. Revelli,O. A. Sbodio,E.J. Tercero
Revista argentina de microbiolog?-a , 2004,
Abstract: Un total de 6.998 muestras de leche cruda de pool de tanque, correspondientes a 55 establecimientos lecheros asociados a la Cooperativa Tambera Nueva Alpina Ltda., fueron recolectadas entre los a os 1993 y 2002. Se realizó el Recuento de Microorganismos Aerobios Mesófilos Totales, obteniéndose como resultado un valor medio de 1,2 x 105± 2,4 x 105UFC/ml que caracteriza la zona. El a o de finalización de la experiencia, se observó un 97% de los tambos evaluados con promedios £ 1,0 x 105UFC/ml. Solamente un 3% sobrepasa este límite, no encontrándose establecimientos con niveles superiores a 1,5 x 105UFC/ml. Al relacionar este indicador con parámetros composicionales, las correlaciones más significativas resultaron para: Recuento de Bacterias Totales vs Acidez (r = 0,292; P < 0,001) y Recuento de Bacterias Totales vs Descenso Crioscópico (r = 0,157; P < 0,001). La calidad microbiológica en leche cruda de tambos que pertenecen a la zona noroeste de Santa Fe y sur de Santiago del Estero, presentó un mejoramiento significativo. A total of 6.998 raw milk samples of bulk tank, belonging to 55 dairy farms associated to the Cooperativa Tambera Nueva Alpina Ltda., were collected between the years 1993 and 2002. The Total Mesophilic Aerobic Microorganisms Count was analyzed, obtaining a medium value of 1.2 x 105± 2.4 x 105CFU/ml that characterizes the zone. The final year of experience, observed a 97% of dairy farms evaluated with averages £ 1,0 x 105CFU/ml. Only a 3% it surpasses this limit, not being found establishments with levels over 1.5 x 105CFU/ml. Relating this indicators with compositionals parameters, the most significant correlation resulted for: Total Bacterial Count vs Acidity (r = 0.292; P < 0.001) and Total Bacterial Count vs Freezing Point (r = 0.157; P < 0.001. The microbiological quality in raw milk of dairy farms belonging to the northwest zone of Santa Fe and south of Santiago del Estero showed a significant improvement during this time.
A non-invasive intratracheal inoculation method for the study of pulmonary melioidosis
David A. Revelli,Julie A. Boylan
Frontiers in Cellular and Infection Microbiology , 2012, DOI: 10.3389/fcimb.2012.00164
Abstract: Pulmonary melioidosis, a disease manifestation caused by the bacterium Burkholderia pseudomallei, has been studied using aerosols or intranasal (IN) inoculation in small animal models. Both have inherent disadvantages which may not accurately model primary pulmonary melioidosis in humans. Intratracheal inoculation (IT) by direct visualization of the tracheal opening offers an alternative technique for infection that overcomes the disadvantages of aerosol and IN challenge. In this study, we describe a method which requires relatively inexpensive equipment, little training, and is compliant with the operational constraints of a BSL3 laboratory. Results obtained using trypan blue demonstrated that an inoculum can be accurately delivered into the lungs of mice within a biosafety cabinet (BSC). Whole body imaging and histopathology confirmed that mice inoculated intratracheally with B. pseudomallei develop the primary focus of infection in the lungs, and not the nasal passages which can lead to invasion of the central nervous system and potential neurologic complications. Further, based on colony counts and bioluminescent imaging, dissemination to secondary organs occurred as expected. Taken together, this intratracheal method of inoculation fulfills four goals: (1) to accurately deliver B. pseudomallei into the lungs of the animal model, (2) to avoid potentially confounding complications due to primary infections at sites other than the lung, (3) to maintain normal organ dissemination, and (4) to be BSL3 compliant.
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