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Search Results: 1 - 10 of 204830 matches for " Francisco de Assis Alves;Mendes "
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Eficiência de isolamento e de plaqueamento de protoplastos de laranja-doce
Castro, Lívia Mendes de;Mour?o Filho, Francisco De Assis Alves;Mendes, Beatriz Madalena Januzzi;
Revista Brasileira de Fruticultura , 2011, DOI: 10.1590/S0100-29452011005000074
Abstract: protoplast isolation and culture are important factors for adequate in vitro culture of this type of explant to further genetic manipulations. the composition of the enzymatic solution, protoplast platting density, and plant genotype are important variables in these steps. therefore, this work aimed to evaluate the isolation efficiency of protoplasts related to three enzymatic solutions and platting efficiency of protoplasts based on five cell densities in different culture media compositions of sweet oranges cultivars. the enzymatic solutions tested were: 1. celulase onozuka rs 1%, macerase r-10 1% and pectoliase 0,2%; 2. celulase onozuka rs 1%, macerase r-10 1% ; 3. celulase onozuka r-10 4% , macerase r-10 1%. protoplasts were cultured at densities of 2 x 104; 5 x 104; 105; 2x 105 e 3 x 105 protoplasts.ml-1 in eme 0,7m, bh3 0,7m e bh3 + eme 0,7m, in darkness, at 25 ± 1 oc. the enzymatic solution 2 provided higher yield for 'hamlin', 'natal' and 'pêra' sweet orange cultivars, and enzymatic solution 1 resulted in better protoplast isolation for 'westin' sweet orange. for 'lima verde' sweet orange, enzymatic solution 3 was the most efficient. final platting efficiency, evaluated 90 days after culture, was higher at the densities of 3 x 105 e 2 x 105 protoplasts.ml-1 for 'hamlin', 'natal', and 'lima verde' sweet orange cultivars, and at the density of 2 x 105 e 105 protoplasts. ml-1 for 'westin' sweet orange.
Plant regeneration from protoplast of Brazilian citrus cultivars
GLORIA, FERNANDA JANUZZI MENDES DA;MOUR?O FILHO, FRANCISCO DE ASSIS ALVES;MENDES, BEATRIZ MADALENA JANUZZI;
Pesquisa Agropecuária Brasileira , 2000, DOI: 10.1590/S0100-204X2000000400008
Abstract: a procedure is described to regenerate plants from protoplasts of brazilian citrus cultivars, after isolation, fusion and culture. protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. the enzyme solution for protoplast isolation was composed of mannitol (0.7 m), cacl2 (24.5 mm), nah2po4 (0.92 mm), mes (6.15 mm), cellulase (onozuka rs - yakult, 1%), macerase (onozuka r10 - yakult, 1%) and pectolyase y-23 (seishin, 0.2%). protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. somatic embryo formation occurred spontaneously after two subcultures, on modified mt medium supplemented with 500 mg/l of malt extract. well defined embryos were germinated in modified mt medium with addition of ga3 (2.0 mm) and malt extract (500 mg/l). plant regeneration was also achieved by adventitious shoots obtained through direct organogenesis of not well defined embryos in modified mt medium with addition of malt extract (500 mg/l), bap (1.32 mm), naa (1.07 mm) and coconut water (10 ml/l). plantlets were transferred to root medium. rooted plants were transferred to a greenhouse for further adaptation and development.
Plant regeneration from protoplast of Brazilian citrus cultivars
GLORIA FERNANDA JANUZZI MENDES DA,MOUR?O FILHO FRANCISCO DE ASSIS ALVES,MENDES BEATRIZ MADALENA JANUZZI
Pesquisa Agropecuária Brasileira , 2000,
Abstract: A procedure is described to regenerate plants from protoplasts of Brazilian citrus cultivars, after isolation, fusion and culture. Protoplasts were isolated from embryogenic cell suspension cultures and from leaf mesophyll of seedlings germinated in vitro. The enzyme solution for protoplast isolation was composed of mannitol (0.7 M), CaCl2 (24.5 mM), NaH2PO4 (0.92 mM), MES (6.15 mM), cellulase (Onozuka RS - Yakult, 1%), macerase (Onozuka R10 - Yakult, 1%) and pectolyase Y-23 (Seishin, 0.2%). Protoplast culture in liquid medium after chemical fusion lead to the formation of callus colonies further adapted to solid medium. Somatic embryo formation occurred spontaneously after two subcultures, on modified MT medium supplemented with 500 mg/L of malt extract. Well defined embryos were germinated in modified MT medium with addition of GA3 (2.0 muM) and malt extract (500 mg/L). Plant regeneration was also achieved by adventitious shoots obtained through direct organogenesis of not well defined embryos in modified MT medium with addition of malt extract (500 mg/L), BAP (1.32 muM), NAA (1.07 muM) and coconut water (10 mL/L). Plantlets were transferred to root medium. Rooted plants were transferred to a greenhouse for further adaptation and development.
Gene silencing: concepts, applications, and perspectives in woody plants
Souza, Amancio José de;Mendes, Beatriz Madalena Januzzi;Mour?o Filho, Francisco de Assis Alves;
Scientia Agricola , 2007, DOI: 10.1590/S0103-90162007000600014
Abstract: rna interference, transcriptional gene silencing, virus induced gene silencing, and micro rnas comprise a series of mechanisms capable of suppressing gene expression in plants. these mechanisms reveal similar biochemical pathways and appear to be related in several levels. the ability to manipulate gene silencing has produced transgenic plants able to switch off endogenous genes and invading nucleic acids. this powerful biotechnological tool has provided plant breeders and researchers with great opportunity to accelerate breeding programs and developmental studies in woody plants. this research work reports on gene silencing in woody plants, and discuss applications and future perspectives.
Indu??o e cultivo in vitro de gemas adventícias em segmentos de epicótilo de laranja-azeda
Silva, Rosely Pereira da;Mendes, Beatriz Madalena Januzzi;Mour?o Filho, Francisco de Assis Alves;
Pesquisa Agropecuária Brasileira , 2008, DOI: 10.1590/S0100-204X2008001000011
Abstract: the objective of this work was to evaluate the induction and formation of adventitious buds in sour orange explants through the use of plant regulators. in vitro organogenesis experiments were conducted to evaluate the effect of bap, tdz, and kin in different concentrations and under two light conditions; bap and kin, combined or not with naa; bap and kin, separately or in combined concentrations. sour orange epicotyl segments (1 cm length), from in vitro germinated plants, were used as explants. in order to induce bud formation, the explants were cultured in mt medium with or without the addition of plant regulators. the material was cultivated at 27oc in the absence of light for 30 days, followed of culture under a 16-hour photoperiod. the experimental design was completely randomized, with four or five replicates, depending on the experiment; each replicate comprised one petri dish with 20 explants. after 60 or 70 days of cultivation, the percentage of responsive explants and the number of buds were evaluated. the addition of bap to the culture medium, combined or not with ana, and in combinations with cin, induces better organogenic response.
Enraizamento de estacas do híbrido somático laranja 'caipira' + lim?o 'Volkameriano' e de seus genitores
Bassan, Meire Menezes;Mour?o Filho, Francisco de Assis Alves;Mendes, Beatriz Madalena Januzzi;
Revista Brasileira de Fruticultura , 2009, DOI: 10.1590/S0100-29452009000200041
Abstract: this work aimed to compare the rooting capacity of cuttings of the somatic hybrid 'caipira' sweet orange (c. sinensis l. osbeck) + 'volkamer' lemon (c. volkameriana v. tennore et pasquale) with those of their progenitors treated with indol butyric acid (iba) (0, 500, 1000 and 2000 mg l-1). cuttings of 'caipira' sweet orange and 'volkamer' lemon were collected from seed derived plants, whereas those of the somatic hybrid were obtained from recently pruned grafted plants. the experimental design was a completely randomized block 3 x 4 factorial (genotypes x iba concentrations), with four replications (10 cuttings per replication) in a total of 480 cuttings. cuttings were cultivated in expanded polystyrene trays filled with commercial potting mix, inside an intermittent mist chamber, for 90 days. the production of the rooted plants was highly efficient for the three genotypes evaluated, considering most of the variables related with rooting and cutting vigor, regardless the use of iba. 'volkamer' lemon cuttings had higher root length and root dry mass than the other genotypes. however, percentage of live cuttings, rooted cuttings and sprouted cuttings were high (above 90%) for all evaluated genotypes, indicating the great potential of this material to be used in nursery tree multiplication through cuttings.
Calogênese, embriogênese somática e isolamento de protoplastos em variedades de laranja doce
Benedito, Vagner Augusto;Mour?o Filho, Francisco de Assis Alves;Mendes, Beatriz Madalena Januzzi;
Scientia Agricola , 2000, DOI: 10.1590/S0103-90162000000100007
Abstract: intending to produce embryogenic citrus calli (citrus sinensis l. osbeck), aborted ovules of ripe fruits from six sweet orange varieties (?bahia cabula?, ?baianinha?, ?hamlin?, ?orvalho de mel?, ?rubi? and ?valencia?) were introduced onto modified mt medium with and without benzyladenine (ba). calli obtained from ?bahia cabula?, ?orvalho de mel?, ?rubi? and ?valencia? varieties were grown on mt basal medium modified with the carbohydrates maltose, galactose, lactose, glucose and sucrose at 18; 37; 75; 110 and 150 mm. the effect on somatic embryogenesis was studied. calli from the same genotypes were submitted to protoplast isolation, using three different enzymatic solutions. results revealed the genotype effect in the callus induction. somatic embryogenesis was stimulated in the presence of galactose, for most varieties. enzymatic solution composed of 1% cellulase, 1% macerase and 0,2% pectolyase was the best for protoplast isolation from calli of all genotypes tested.
Calogênese, embriogênese somática e isolamento de protoplastos em variedades de laranja doce
Benedito Vagner Augusto,Mour?o Filho Francisco de Assis Alves,Mendes Beatriz Madalena Januzzi
Scientia Agricola , 2000,
Abstract: Com o objetivo de produzir calos embriogênicos de citros, óvulos abortados de frutos maduros de 6 variedades de laranja doce (Citrus sinensis L. Osbeck), ?Bahia Cabula?, ?Baianinha?, ?Hamlin?, ?Orvalho de Mel?, ?Rubi? e ?Valência? foram introduzidos em meio de cultivo MT modificado com adi o de extrato de malte, com e sem adi o de 5 mg L-1 benziladenina (BA). Os calos obtidos das variedades ?Bahia Cabula?, ?Orvalho de Mel?, ?Rubi? e ?Valência? foram cultivados em meio basal MT, contendo os carboidratos maltose, galactose, lactose, glicose ou sacarose nas concentra es de 18; 37; 75; 110 e 150 mM para a indu o da embriogênese somática. Os calos destas mesmas variedades foram submetidos ao isolamento de protoplastos, com o uso de 3 solu es enzimáticas. Os resultados revelaram que há influência do genótipo sobre a calogênese. A embriogênese somática foi estimulada na presen a de galactose, para a maioria das variedades. A solu o enzimática composta de 1% de celulase, 1% de macerase e 0,2% de pectoliase foi a mais adequada para o isolamento de protoplastos a partir de calos das variedades de laranja doce estudadas.
Embriogênese somática a partir de calos de cultivares de laranja doce
Castro, Lívia Mendes de;Mour?o Filho, Francisco de Assis Alves;Mendes, Beatriz Madalena Januzzi;Miyata, Luzia Yuriko;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010000800026
Abstract: plant regeneration, by organogenesis or somatic embryogenesis, from cell cultures and in vitro plant tissue culture is the basis for biotechnology usage in plant breeding. this research aimed to evaluate somatic embryogenesis from embryogenic calli of 'hamlin', 'pêra', 'natal', 'lima verde', and 'westin' sweet orange cultivars related to culture medium composition as source and concentration of carbohydrates with the use of mt culture medium modified with 500mg l-1 of malt extract, supplemented with sucrose, galactose, glucose, maltose, lactose, or sorbitol at concentrations of 18, 37, 75, 110 or 150mm at 27°c. statistical design were complete randomized, in a factorial five (cultivars) x six (carbohydrate source) x six (carbohydrate concentration in culture medium) with five replicates. the production of somatic embryos varied with the genotype, as 'hamlin' and 'natal' cultivars registered higher number of embryos, whereas 'lima verde' and 'westin' showed lower numbers. the best carbohydrate source was maltose, followed by lactose at concentrations of 37 and 75mm. somatic embryogenesis was not observed on culture media supplemented with galatose, glucose or sorbitol in any studied cultivar.
In vitro organogenesis in some citrus species
Schinor, Evandro Henrique;Azevedo, Fernando Alves de;Mour?o Filho, Francisco de Assis Alves;Mendes, Beatriz Madalena Januzzi;
Revista Brasileira de Fruticultura , 2011, DOI: 10.1590/S0100-29452011005000050
Abstract: in vitro organogenesis of citrus was studied for the genotypes citrus sinensis cv. 'natal', c. limonia, c. volkameriana, and c. aurantium, with the use of epicotyl segments-derived explants, cultured in mt salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (bap - 0.0; 0.5; 1.0; 1.5 or 2.0 mg l-1). for the recalcitrant genotypes c. limonia and c. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in mt salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg l-1 of bap. the efficiency of culture medium supplementation with the combination of bap (0.0; 1.0, or 2.0 mg l-1) and naa (1-naphthaleneacetic acid - 0.0; 0.3, or 0.5 mg l-1) in the development of adventitious shoots was evaluated for c. aurantium. culture medium supplementation with bap is not essential for the adventitious shoots development in the four genotypes studied when epicotyl segments-derived explants are used. in general, culture media supplementation with bap decreased the percentage of responsive explants excepted for c. sinensis cv. 'natal' and c. limonia when the concentrations of 1.5 and 2.0 mg/l were used. the presence of cytokinin, in concentrations up to 2 mg/l, stimulated the in vitro organogenesis when internodal segments-derived explants were used for c. limonia and c. aurantium. for c. aurantium no adventitious shoots developed in explants (internodal segments) cultured in basal culture medium, without bap supplementation. although no statistic differences could be detected, culture media supplementation with the combination of bap and naa favored the development of adventitious shoots in c. aurantium. the best concentration of naa varied according to bap concentration. the results presented herein, show that citrus in vitro organogenesis depends on the interaction of culture medium composition, explant differentiation level, and genotype.
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