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Search Results: 1 - 10 of 58762 matches for " Flávia Galindo Silvestre Silva "
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Prevalence of Human Leukocyte Antigen HLA-B*5701 in HIV-1 Infected Individuals in Brazil  [PDF]
Claudinéia de Araújo, Cristina Valetta de Carvalho, Miriam Estela de Souza Freire, Amanda Yamaguti, Ivens Cuiabano Scaff, Fernando José de Souza, Flávia Galindo Silvestre Silva, Ricardo Sobhie Diaz, Ismael Dale Cotrim Guerreiro da Silva
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.41008
Abstract:

This study was designed to establish the prevalence of HLA-B*5701 at HIV-1 infected individuals in Brazil. A total of 517 consecutive individuals were followed in this study from February 2009 through July 2011. The presence of HLA-B*5701 was determined by Nested-PCR with HLA-B*57 and HLA-B*5701 sequence-specific primers (PCR-SSP). The expression of HLA-B*57 was negative in the 385 (74.5%) and positive in the 103 (19.9%) of infected individuals. Among these, the expression of HLA-B5701 was positive in the 29 (5.6%) of individuals. No demographic or ethnic differences were found between HLA-B*57/HLA-B*5701 HIV-1 negative patients, with a prevalence of Caucasians (57.1%) individuals. During the period of study, 68 patients were submited to an abacavir contain- ing regimen. The HLA-B*5701 allele was observed in 7 (10.3%) patients, with a significant incidence of Hypersensitivity reactions at 4 of them (p < 0.001). Conclusions: Although Brazilian population consists of a mixture of individuals of Caucasian, African and Native American genetic background, prevalence of HLA-B*5701 in this population is similar to the one found in pure Caucasians.


Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
Figueiredo, Eduardo Eustáquio de Souza;Carvalho, Ricardo Cezar Tavares;Silvestre, Flávia Galindo;Lilenbaum, Walter;Fonseca, Leila Sousa;Silva, Joab Trajano;Paschoalin, Vania Margaret Flosi;
Brazilian Journal of Microbiology , 2010, DOI: 10.1590/S1517-83822010000200020
Abstract: detection of tuberculosis in cattle relies on the intradermal tuberculin test (itt), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. dna in nasal swabs from 50 cows was analyzed by m-pcr, targeting for the rvd1-rv2031c and is6110 sequences. m. bovis was identified in two of 34 tuberculous cows (5.9%). the use of mpcr of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
Identification of Mycobacterium bovis Isolates by a multiplex PCR
Figueiredo, Eduardo Eustáquio de Souza;Silvestre, Flávia Galindo;Campos, Wilma Neres;Furlanetto, Leone Vinícius;Medeiros, Luciana;Lilenbaum, Walter;Fonseca, Leila Sousa;Silva, Joab Trajano;Paschoalin, Vania Margaret Flosi;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000200004
Abstract: isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-pcr) targeting for rvd1rv2031c and is6110 sequences, specific for m. bovis and mycobacterium tuberculosis complex respectively. the m-pcr successfully identified as m. bovis 88.24% of the isolates.
O Programa de Estágio Monitorado na pós-gradua??o lato sensu em medicina do trabalho
Silva-Júnior, Jo?o Silvestre da;Almeida, Flávia Souza e Silva de;Morrone, Luiz Carlos;
Revista Brasileira de Educa??o Médica , 2011, DOI: 10.1590/S0100-55022011000300004
Abstract: context: in 2004, a proposal was introduced to restructure an executive master's course in occupational medicine, with an increase in the practical course load. the school of medicine at the s?o paulo mercy hospital (s?o paulo, brazil) introduced the monitored internship program to complement the basic 680 class hours. objectives: the study's objectives were to describe the program and the course coordinator's perceptions of it and to analyze course alumni's current relationship to the occupational medicine specialty. material and methods: (a) unstructured discussion among coordinators and an interview with partner companies; (b) application of a questionnaire to 46 course graduates, discussing social and professional issues. results: the monitored internship program has a 1,240 hour course load, participation is voluntary and based on double monitoring of the student's activities, with the submission of weekly reports for clinical discussion. the study detected increasing interest in the theoretical/practical course and a lack of programs for professional development in the internship sites. among the sample, 67.3% were meeting a weekly workload of greater than 20 hours in occupational medicine. discussion: the program meets the established guidelines and appears to help retain course participants within the specialty. the study raises the issue of the inability of the work market in this specialty to provide sufficient sites for more solid practical training and recommends discussing the specialty's regulation.
A EDUCA O ALEM NA COL NIA RIOGRANDENSE: 1922-1938 (MARACAí/CRUZáLIA-SP)
Flávia Silva
Nuances : Estudos sobre Educa??o , 2012,
Abstract:
Chemical Composition of the Cashew Apple Bagasse and Potential Use for Ethanol Production  [PDF]
Flávia Cristina dos Santos Lima, Flávio Luiz Honorato da Silva, Josivanda Palmeira Gomes, José Mariano da Silva Neto
Advances in Chemical Engineering and Science (ACES) , 2012, DOI: 10.4236/aces.2012.24064
Abstract: On the world scene, the energy requirements are mainly based on fossil fuels, however, these compounds reserves are finite and their exploitation has caused serious environmental problems. As a consequence, the demand for alternative renewable sources has been intensified in substitution the rising demand for energy and raw materials. The biomass is emerging as one of the few sources that have potential to meet these challenges of sustainability, as is currently the largest energy resource in the world, and only carbon-rich material available on the planet, apart from fossils. Form, the cashew crop has great potential for technological development of alternative sources of energy, from its industrial waste processing cashew adding value to the product. In this sense, this paper aims to study the characterization of the cashew apple bagasse and to verify (by acid prehydrolysis) the potential of this material for ethanol production. Initially it was carried out physicochemical characterization of cashew bagasse used (pH, moisture content, soluble solids, sugars, cellulose, hemicellulose and lignin). Following it was carried prehydrolysis at 105℃ for 1h to obtain fermentable sugars. Analyses of the samples were carried out on HPLC the results showed the saccharification of biomass with glucose (1537.49 mg/L), xylose (3823.22 mg/L) and arabinose (7131.11 mg/L) as well as the capacity of the biomass for ethanol production.
Patogenicidade de Corynespora cassiicola a diferentes espécies de plantas
Cutrim, Flávia A.;Silva, Gilson S.;
Fitopatologia Brasileira , 2003, DOI: 10.1590/S0100-41582003000200014
Abstract: two isolates of corynespora cassiicola obtained from tomato (lycopersicon esculentum) were tested in a greenhouse to determine their pathogenicity to squash (cucurbita pepo), barbados cherry (malpighia glabra), cowpea (vigna unguiculata), papaya (carica papaya), west indian gherkin (cucumis anguria), pepper (capsicum annum), okra (abelmoschus esculentus), soybean (glycines max), tomato and sorrel (hibiscus sabdariffa). the purpose of the study was to select plants for crop-rotation systems in tomato plantations.two weeds common to tomato plantations, commelina benghalensis and vernonia cinerea, were included to evaluate their importance as source of inoculum. the host plants tested reacted differently to the pathogen but most of them were susceptible to both isolates. this suggests that tomato plants should not be cultivated in association with or closely to squash, cucumber, pepper, okra and sorrel plantations, especially where c. cassiicola occurs. for controlling the weeds c. benghalensis and v. cinerea, it is fundamentally important reduce inoculum in the field.
Interferência do ácido ascórbico nas determina??es de parametros bioquímicos séricos: estudos in vivo e in vitro
Martinello, Flávia;Silva, Edson Luiz da;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2003, DOI: 10.1590/S1676-24442003000400010
Abstract: background: ascorbic acid, when present in biological samples, can produce a negative interference in several biochemical tests that use redox indicator systems. objectives: we evaluated the ascorbic acid interference on serum biochemical tests in relation to the dose of vitamin c ingested and to the time of blood collection, and compared these results with in vitro interference. methods: healthy volunteers (n =18) consumed increasing and successive doses of vitamin c (0.25 to 4g/day) for one week. biochemical analytes were measured before and after each dose consumption. serum samples were obtained 4, 12 and 24 h after vitamin c ingestion. ascorbic acid was added in vitro to the human serum and the biochemical analytes were assayed up to 24 h after addition. results and discussion: serum ascorbic acid increased significantly after vitamin c ingestion, inhibiting the assays for urate and total bilirubin up to 24 h after ingestion (p < 0,01). in contrast, the ingested vitamin c did not interfere on the glucose, cholesterol, and triglycerides tests. in vitro ascorbic acid inhibited the reactions for all studied tests, proportional to the ascorbic acid amount and inversely proportional to the analyte concentrations. the urate test had in vitro interference only, whereas for bilirubin there were in vitro and, mainly, in vivo interference. conclusion: to avoid false-negative results of serum urate and bilirubin we recommend the suspension of vitamin c intake at least 48 horas before blood collection.
Indicadores para monitoramento de pesquisa em saúde no Brasil
Elias, Flávia Silva;Souza, Luis;
Ciência da Informa??o , 2006, DOI: 10.1590/S0100-19652006000300021
Abstract: in brazil, the ministry of health is an important financier and consumer of research and projects on technological assessment. however, the formal mechanisms of accompaniment of the promotion to research are incipient. the aim of this work was to propose indicators to monitor the promotion of the financed researches. information needs were identified and indicators were elaborated based on visits to institutions for research promotion in brazil, literature review and technical meetings. the indicators answer witch research are financed and what is the correlation to priorities to health polities; how the financing distribution occurs, witch externalities were produced by research.. it is discussed the importance of monitoring to designate resources within priority research. the use of indicators can guide the construction of a database for the ministry of health.
A pesquisa sobre a atividade pedagógica: contribui??es da teoria da atividade
Asbahr, Flávia da Silva Ferreira;
Revista Brasileira de Educa??o , 2005, DOI: 10.1590/S1413-24782005000200009
Abstract: the purpose of this article is to present some of the contributions from the psychological theory of activity (vigotski, leontiev, luria, davidov amongst others) to research on teaching activity. the focus of these authors, especially leontiev and davidov, is the study of human activity as the central unit of the concrete individual's life, and its link with consciousness. according to the authors, in a class society, human consciousness becomes fragmented and disintegrates; social significance and personal meanings, structural components of consciousness, are not just coincidences but become contradictory. based on these reflections, we asked what would be the consequences of the rupture between meanings and senses in pedagogical activity and what would be the possibilities of coincidence between these two dimensions of human consciousness in pedagogical work. to answer these questions, we sought, first of all, to delimit the meaning of the teacher's pedagogical activity, focusing on the propositions of historical cultural psychology and critical historical pedagogy. this was a necessary condition in order to be able to research the personal meaning of the teacher's pedagogical activity, or what motivates teaching activity. we then briefly analysed the implications of the rupture between the significance and personal meaning of pedagogical activity. finally, we pointed to some possibilities for resistance to this rupture at the theoretical level.
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