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Search Results: 1 - 10 of 40526 matches for " Fernando Augusto Soares "
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Optimization of an Alternative Methodology for Simultaneous Analysis of Nitrite and Nitrate in Water from Urban Stream by Capillary Electrophoresis under Direct UV Detection  [PDF]
Marcone Augusto Leal de Oliveira, Denise do Carmo Soares, Gláucia Soares Tostes, Mara do Carmo Guimar?es, Fernando Antonio Simas Vaz
American Journal of Analytical Chemistry (AJAC) , 2012, DOI: 10.4236/ajac.2012.37064
Abstract: An alternative methodology for simultaneous determination of nitrite and nitrate by capillary zone electrophoresis using direct detection UV at 210 nm under reverse electrosmotic flow is proposed. The choice of the electrolyte composition has taken account: the mobility of the anion buffer and of the solutes; the low absorbance of the buffer in 210 nm; high base line stability and analysis time. The electrolyte optimized has consisted of 100 mmol.L–1 TRIS/HCl buffer and 0.15 mmol.L–1 CTAB at pH 8.2. The proposed method was applied successfully in the analysis of nitrite and nitrate in samples from urban stream in the absence of usual sample pretreatment.
Matrix Metalloproteinase-9 Expression by Hodgkin-Reed-Sternberg Cells Is Associated with Reduced Overall Survival in Young Adult Patients with Classical Hodgkin Lymphoma
Antonio Hugo Campos, Jose Vassallo, Fernando Augusto Soares
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074793
Abstract: Previous studies have investigated the prognostic relevance of MMP9 in classical Hodgkin lymphoma (cHL), with negative results. However, we have found that MMP9 immunoistochemical expression by Hodgkin-Reed-Sternberg cells is associated with reduced overall survival in a subset of young adult Brazilian patients diagnosed with cHL. Additionally, we have observed that MMP9 expression by neoplastic cells in cHL is associated with EBV positivity. These results may support a rational basis for additional studies on the role of this metalloproteinase as a target for therapy in classical Hodgkin lymphoma.
Avalia??o de resposta patológica em cancer de mama após quimioterapia neoadjuvante: padroniza??o de protocolo adaptado
Osório, Cynthia Aparecida Bueno Toledo;Chaves Júnior, Marcos Araújo;Soares, Fernando Augusto;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2012, DOI: 10.1590/S1676-24442012000600010
Abstract: the concept of pathologic complete response (pcr) is controversial. previous studies used different methods of pcr assessment, but there is no universal consensus about the best protocol for the study of surgical specimens from patients with breast carcinoma undergoing neoadjuvant chemotherapy. symmans et al. developed a classification system of residual cancer burden (rcb) after neoadjuvant chemotherapy for breast cancer analyzing the extent of primary tumor bed, the percentage of residual viable tumor cells in the tumor bed and lymph node involvement. we present a proposal for the evaluation of pathological response in breast tumors after neoadjuvant chemotherapy. based on symmans et al. study, it consists in a protocol adapted to our routine pathological examination of surgical specimens.
Detec??o de micrometástases em cancer de pulm?o n?o-pequenas células estádio pN0: um método alternativo combinando imunohistoquímica e análise em microsséries
Franco, Maíra Rovigatti;Parra, Edwin Roger;Takagaki, Teresa Yae;Soares, Fernando Augusto;Capelozzi, Vera Luiza;
Jornal Brasileiro de Pneumologia , 2008, DOI: 10.1590/S1806-37132008000300002
Abstract: objective: to present an alternative method of detecting micrometastases in lymph nodes previously testing negative for non-small cell lung cancer (nsclc) by routine hematoxylin-eosin staining. methods: a total of 77 hilar and mediastinal lymph nodes resected from 18 patients with nsclc were investigated for the presence of micrometastases using a combination of microarray analysis and immunohistochemistry. results: micrometastases were detected by identifying cytokeratin- and chromogranin-positive cells in lymph node microarrays. of the 18 patients initially staged as pn0 through routine hematoxylin-eosin staining, 9 (50%) were restaged as n1, and the prognoses were re-evaluated in terms of histological and clinical parameters. the comparison of the survival curves revealed that survival was higher in the patients without micrometastases than in those with micrometastases. in addition, in the multivariate analysis adjusted for age, gender, histological type, and restaging, the presence of micrometastases proved to be an independent predictor of survival. among patients who had been previously staged as pn0, the risk of death was found to be 7-times greater for those later diagnosed with micrometastases than for those in whom no micrometastases were identified. conclusion: the combination of microarray analysis and immunohistochemistry might represent a low-cost and less time-consuming alternative for identifying occult micrometastases and predicting prognoses in surgically resected patients with pn0 nsclc. larger randomized, prospective studies are needed in order to determine the accuracy of this method.
Histiocitose de células de Langerhans com envolvimento ungueal em crian?a: relato de caso
Mendes, Wellington Luiz;Melotti, Claudia Zavaloni;Bezerra, Alanna Mara;Soares, Fernando Augusto;Camargo, Beatriz de;
Anais Brasileiros de Dermatologia , 2006, DOI: 10.1590/S0365-05962006000600007
Abstract: langerhans cell histiocytosis may appear in a variety of ways. the authors present the case report of a 3-year-old white boy with a main complaint about chronic media otitis and a tumor lesion in right mastoid bone. pathology revealed histiocytosis. the patient had severe dermatitis on the scalp and dystrophic changes with onycholysis, pustules, and nail plate deformity underneath all fingernails and toenails. these lesions responded to antineoplastic therapy. development of nail dystrophics in langerhans cell histiocytosis is unusual in children. this case suggests that treatment with antineoplastic therapy might be effective.
Heterogeneous geographic distribution of human T-cell lymphotropic viruses I and II (HTLV-I/II): serological screening prevalence rates in blood donors from large urban areas in Brazil
Catalan-Soares, Bernadette;Carneiro-Proietti, Anna Bárbara de F.;Proietti, Fernando Augusto;,;
Cadernos de Saúde Pública , 2005, DOI: 10.1590/S0102-311X2005000300027
Abstract: brazil may have the highest absolute number of htlv-i/ii seropositive individuals in the world. screening potential blood donors for htlv-i/ii is mandatory in brazil. the public blood center network accounts for about 80.0% of all blood collected. we conducted a cross-sectional study to assess the geographic distribution of htlv-i/ii serological screening prevalence rates in blood donors from 27 large urban areas in the various states of brazil, from 1995 to 2000. enzyme immunoassay (eia) was used to test for htlv-i/ii. the mean prevalence rates ranged from 0.4/1,000 in florianópolis, capital of santa catarina state, in the south, to 10.0/1,000 in s?o luiz, maranh?o state, in the northeast. eia prevalence rates are lower in the south and higher in the north and northeast. the reasons for such heterogeneity may be multiple and need further studies.
Foxp3 expression is associated with aggressiveness in differentiated thyroid carcinomas
Cunha, Lucas Leite;Morari, Elaine Cristina;Nonogaki, Suely;Soares, Fernando Augusto;Vassallo, José;Ward, Laura Sterian;
Clinics , 2012, DOI: 10.6061/clinics/2012(05)13
Abstract: objectives: forkhead box p3 (foxp3) expression has been observed in human cancer cells but has not yet been reported in thyroid cells. we investigated the prognostic significance of both foxp3 expression and intratumoral foxp3+ lymphocyte infiltration in differentiated thyroid carcinoma cells. methods: we constructed a tissue microarray with 385 thyroid tissues, including 266 malignant tissues (from 253 papillary thyroid carcinomas and 13 follicular carcinomas), 114 benign lesions, and 5 normal thyroid tissues. results: we determined the expression of foxp3 in both tumor cells and tumor-infiltrating lymphocytes using immunohistochemical techniques. cellular expression of foxp3 was evident in 71% of benign and 91.9% of malignant tissues. the nuclear and cytoplasmic expression patterns were quantified separately. a multivariate logistic regression analysis indicated that cytoplasmic foxp3 expression is an independent risk factor for thyroid malignancy. cytoplasmic foxp3 staining was inversely correlated with patient age. nuclear foxp3 staining was more intense in younger patients and in tumors presenting with metastasis at diagnosis. foxp3+ lymphocytes were more frequent in tumors smaller than 2 cm, those without extrathyroidal invasion, and in patients with concurrent chronic lymphocytic thyroiditis. conclusions: we demonstrated foxp3 expression in differentiated thyroid carcinoma cells and found evidence that this expression may exert an important influence on several features of tumor aggressiveness.
NDRG1 protein overexpression in malignant thyroid neoplasms
Gerhard, Renê;Nonogaki, Suely;Fregnani, José Humberto Tavares Guerreiro;Soares, Fernando Augusto;Nagai, Maria Aparecida;
Clinics , 2010, DOI: 10.1590/S1807-59322010000800003
Abstract: objectives: the aim of this study was to examine the expression of the n-myc downstream-regulated gene 1 protein in benign and malignant lesions of the thyroid gland by immunohistochemistry. introduction: n-myc downstream-regulated gene 1 encodes a protein whose expression is induced by various stimuli, including cell differentiation, exposure to heavy metals, hypoxia, and dna damage. increased n-myc downstream-regulated gene 1 expression has been detected in various types of tumors, but the role of n-myc downstream-regulated gene 1 expression in thyroid lesions remains to be determined. methods: a tissue microarray paraffin block containing 265 tissue fragments corresponding to normal thyroid, nodular goiter, follicular adenoma, papillary thyroid carcinoma (classical pattern and follicular variant), follicular carcinoma, and metastases of papillary and follicular thyroid carcinomas were analyzed by immunohistochemistry using a polyclonal anti- n-myc downstream-regulated gene 1 antibody. results: the immunohistochemical expression of n-myc downstream-regulated gene 1 was higher in carcinomas compared to normal thyroid glands and nodular goiters, with higher expression in classical papillary thyroid carcinomas and metastases of thyroid carcinomas (p < 0.001). a combined analysis showed higher immunohistochemical expression of ndrg1 in malignant lesions (classical pattern and follicular variant of papillary thyroid carcinomas, follicular carcinomas, and metastases of thyroid carcinomas) compared to benign thyroid lesions (goiter and follicular adenomas) (p = 0.043). in thyroid carcinomas, n-myc downstream-regulated gene 1 expression was significantly correlated with a more advanced tnm stage (p = 0.007) and age, metastasis, tumor extent, and size (ames) high-risk group (p = 0.012). conclusions: thyroid carcinomas showed increased immunohistochemical n-myc downstream-regulated gene 1 expression compared to normal and benign thyroid lesions and is correlated with more a
Evaluation of prognostic factors in stage IIA breast tumors and their correlation with mortality risk
Carvalho, Solange Torchia;Stiepcich, Monica Maria;Fregnani, José Humberto;Nonogaki, Sueli;Rocha, Rafael;Soares, Fernando Augusto;
Clinics , 2011, DOI: 10.1590/S1807-59322011000400014
Abstract: breast tumors exhibit extensive molecular and clinical heterogeneity. one of the most utilized breast carcinoma classifications is based on its molecular aspects and subdivides breast cancer into five major groups based on the expression of certain genes. in this study, we evaluated which factors are important in determining a prognosis after 5 years of follow-up for patients with clinical stage iia breast tumors. we took into consideration the different phenotypes (luminal a luminal b her-2 overexpression, basal and triple-negative), various epithelial-mesenchymal (emt) molecular markers and adhesion molecules (e-cadherin, p-cadherin, n-cadherin, vimentin, twist snail and slug) and nos-2, in addition to clinical and demographic data, tumor characteristics and treatment types. methods: the study population consisted of 82 patients with breast cancer. we analyzed eight molecular markers by immunohistochemistry on tissue microarrays containing breast tumor specimens from patients with ten years of follow-up, and we classified each tumor according to its estrogen receptor, progesterone receptor and her-2 expression. we then placed the tumor into one of the above categories. results: the presence of several clinical and demographic factors, various histopathologies, treatment forms and several immunohistochemical markers were not associated with a worse prognosis for group iia patients. the factors that were associated with a mortality risk were the triple-negative (odds ratio (or) = 11.8, 95% confident interval (ci) = 2.0-70.3, p = 0.007) and basal (or =18.4, 95% ci = 1.8-184.7, p= 0.013) phenotypic patterns. conclusions: the emt markers and nos-2 were not mortality risk factors. basal and triple-negative phenotypic patterns were related to a higher mortality risk in patients with stage iia tumors.
HTLV-I/II and blood donors: determinants associated with seropositivity in a low risk population
Soares,Bernadette Catalan; Proietti,Anna Bárbara de F Carneiro; Proietti,Fernando Augusto; ,;
Revista de Saúde Pública , 2003, DOI: 10.1590/S0034-89102003000400012
Abstract: objective: blood donors in brazil have been routinely screened for htlv-i/ii since 1993. a study was performed to estimate the prevalence of htlv-i/ii infection in a low risk population and to better understand determinants associated with seropositivity. methods: htlv-i/ii seropositive (n=135), indeterminate (n=167) and seronegative blood donors (n=116) were enrolled in an open prevalence prospective cohort study. a cross-sectional epidemiological study of positive, indeterminate and seronegative htlv-i/ii subjects was conducted to assess behavioral and environmental risk factors for seropositivity. htlv-i/ii serological status was confirmed using enzyme-linked immunosorbent assay (eia) and western blot (wb). results: the three groups were not homogeneous. htlv-i/ii seropositivity was associated to past blood transfusion and years of schooling, a marker of socioeconomic status, and use of non-intravenous illegal drugs. conclusions: the study results reinforce the importance of continuous monitoring and improvement of blood donor selection process.
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