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Search Results: 1 - 10 of 788319 matches for " Fernanda A. G.;Silva Júnior "
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Dieta de Micoureus demerarae (Thomas) (Mammalia, Didelphidae) associada às florestas contíguas de mangue e terra firme em Bragan?a, Pará, Brasil
Fernandes, Marcus E. B.;Andrade, Fernanda A. G.;Silva Júnior, José de S. e;
Revista Brasileira de Zoologia , 2006, DOI: 10.1590/S0101-81752006000400015
Abstract: the diet of micoureus demerarae (thomas, 1905) was studied in mangrove and terra firme stands by using stomachal and faecal samples. the number of captured individuals was inversely proportional to availability of fruits and insects, being coleoptera and hemiptera the most consumed arthropod orders and passifloraceae and arecaceae the most ingested fruits. thus, either fruits variability or their high yield during the dry season seem to explain the increase of captured animals in the terra firme stands, where they originally come from. the food items suggest that this species has an omnivorous diet, independently of the seasonality or distribution of available resources.
Production, Characterization and Applications for Toxoplasma gondii-Specific Polyclonal Chicken Egg Yolk Immunoglobulins
álvaro Ferreira Júnior, Fernanda M. Santiago, Murilo V. Silva, Flávia B. Ferreira, Arlindo G. Macêdo Júnior, Caroline M. Mota, Matheus S. Faria, Hercílio H. Silva Filho, Deise A. O. Silva, Jair P. Cunha-Júnior, José R. Mineo, Tiago W. P. Mineo
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040391
Abstract: Background Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. Unfortunately, mammalian antibodies present limitations for its production, such as the necessity for animal bleeding, low yield, interference with rheumatoid factor, complement activation and affinity to Fc mammalian receptors. IgY antibodies avoid those limitations; therefore they could be an alternative to be applied in T. gondii model. Methodology/Principal Findings In this study we immunized hens with soluble tachyzoite antigens of T. gondii (STAg) and purified egg yolk antibodies (IgY) by an inexpensive and simple method, with high yield and purity degree. IgY anti-STAg antibodies presented high avidity and were able to recognize a broad range of parasite antigens, although some marked differences were observed in reactivity profile between antibodies produced in immunized hens and mice. Interestingly, IgY antibodies against Neospora caninum and Eimeria spp. did not react to STAg. We also show that IgY antibodies were suitable to detect T. gondii forms in paraffin-embedded sections and culture cell monolayers. Conclusions/Significance Due to its cost-effectiveness, high production yield and varied range of possible applications, polyclonal IgY antibodies are useful tools for studies involving T. gondii.
Efeito hepatoprotetor do consumo cr?nico de dieptanoína e trieptanoína contra a esteatose em ratos
Silva, Maria A. Firmino da;Ataide, Terezinha da R.;Oliveira, Suzana L. de;Sant'Ana, Antonio E. G.;Cabral Júnior, Cyro R.;Balwani, Maria do C. L. V.;Oliveira, Fernanda G. S. de;Santos, Mércia C.;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2008, DOI: 10.1590/S0004-27302008000700011
Abstract: objective: to evaluate the effect of chronic consumption of di- and triheptanoin on hepatic steatosis (hs) in rats. methodology: wistar rats were submitted to a diet ain-93 with 0, 30 or 50% of its oil substituted with an oil rich in di- and triheptanoin, groups tagc70, tagc730 and tagc750 respectively, for nine months. the control group received labina?. liver histology, hepatic lesion and function proofs, glycemia and lipid profile, were performed. variance analyses, f-test, dunnet′s test and uni- and multivariate regression analyses were performed (p<0.05). results: tagc70, tagc730 and tagc750 developed hs; 80% of severe cases in tagc70, as against 40% in tagc750. the absolute (alw) and relative (rlw) liver weights were higher in tagc70 and tagc730, and glycemia was greater in tagc730 and tagc750, than in the control. total cholesterol, ldl-c, ldl-c/hdl-c and total proteins were higher in the control. the experimental oil reduced rlw and showed a tendency in the reduction of body weight, alw, percentage of hepatic lipids and the severity of hs. the explanatory variables in relation to hs were final weight, glycemia, albumin, hdl-c, ldl-c, ldl-c/hdl-c, vldl-c and alkaline phosphatase. conclusions: it is suggested that di- and triheptanoin have a hepatoprotector effect against hs, in rats, in a dose-dependant manner.
Percep??o das condi??es de saúde bucal por gestantes atendidas em uma unidade de saúde no município de Araraquara, S?o Paulo, Brasil
Silva, Silvio Rocha Corrêa da;Rosell, Fernanda Lopez;Valsecki Júnior, Aylton;
Revista Brasileira de Saúde Materno Infantil , 2006, DOI: 10.1590/S1519-38292006000400007
Abstract: objectives: to assess oral healthcare conditions of a group of pregnant women through the application of the general oral health assessment index (gohai). methods: fifty three pregnant women clients of the basic oral health clinic in araraquara s?o paulo were enrolled in the program. a questionnaire with the gohai rating questions, questions related to self-perception of oral conditions and to social and demographic characteristics was applied. through these mann-whitney and kruskall-wallis non-paramedic tests, the association of social variables and self-perception of the gohai rates were determined. results: oral conditions perception as measured by the gohai index was positive and presented a median value of 31,6. subjective data indicated that only 12,0% of the pregnant women rated their oral condition as "bad", the majority did not disclose any dental problem, although 58,7% reported gum problems. issues such as pain and/or discomfort were the ones more closely perceived by the pregnant women. conclusions: pregnant women had a positive assessment of their oral conditions with the gohai rating associated to self-perception variables. this index could be applied to population groups such as pregnant women, enabling educational and/or preventive measures focused on their real needs.
Caracteriza??o das vaz?es em uma pequena bacia hidrográfica do Distrito Federal, Brasil
Souza, Fernanda A. O. de;Silva, Cícero L. da;Maggiotto, Selma R.;Oliveira Júnior, Manuel P. de;
Revista Brasileira de Engenharia Agrícola e Ambiental , 2012, DOI: 10.1590/S1415-43662012000100002
Abstract: in brazil, distrito federal region is characterized by the presence of streamlets and small rivers, which explains the importance of the discharge characterization in watersheds. this study used 13 years of hydrological data of the capetinga watershed to analyze: the stationarity of hydrological parameters; the statistical distributions that best represent the discharge; the comparison of using short and medium series to calculate discharge; and the requirement for water rights concession according to the current legislation. the lognormal 3 and pearson 3 distributions best represented the maximum discharge, while logpearson 3 distribution was best indicated for minimum discharge. total annual precipitation, long duration mean discharge and seven-day minimum flow presented stationary series, but maximum instantaneous discharge did not. the use of short series to calculate discharge in this watershed was not reasonable, since it resulted in overestimation of the minimum discharge and underestimation of the maximum discharge. according to the legislation, the water capturing from the stream capetinga requires a concession from the regulatory agency, in spite of the distributions minimum discharge has a small value, which reflects its low potential of use.
Evaluation of the activated charcoals and adsorption conditions used in the treatment of sugarcane bagasse hydrolysate for xylitol production
Marton, J. M.;Felipe, M. G. A.;Almeida e Silva, J. B.;Pessoa Júnior, A.;
Brazilian Journal of Chemical Engineering , 2006, DOI: 10.1590/S0104-66322006000100002
Abstract: xylitol has sweetening, anticariogenic and clinical properties that have attracted the attention of the food and pharmaceutical industries. the conversion of sugars from lignocellulosic biomass into xylitol by d-xylose-fermenting yeast represents an alternative to the chemical process for producing this polyol. a good source of d-xylose is sugarcane bagasse, which can be hydrolyzed with dilute acid. however, acetic acid, which is toxic to the yeast, also appears in the hydrolysate, inhibiting microbe metabolism. xylitol production depends on the initial d-xylose concentration, which can be increased by concentrating the hydrolysate by vacuum evaporation. however, with this procedure the amount of acetic acid is also increased, aggravating the problem of cell inhibition. hydrolysate treatment with powdered activated charcoal is used to remove or decrease the concentration of this inhibitor, improving xylitol productivity as a consequence. our work was an attempt to improve the fermentation of candida guilliermondii yeast in sugarcane bagasse hydrolysate by treating the medium with seven types of commercial powdered activated charcoals (synth, carbon delta a, carbon delta g, carbon 117, carbon 118l, carbon 147 and carvorite), each with its own unique physicochemical properties. various adsorption conditions were established for the variables temperature, contact time, shaking, ph and charcoal concentration. the experiments were based on multivariate statistical concepts, with the application of fractional factorial design techniques to identify the variables that are important in the process. subsequently, the levels of these variables were quantified by overlaying the level curves, which permitted the establishment of the best adsorption conditions for attaining high levels of xylitol volumetric productivity and d-xylose-to-xylitol conversion. this procedure consisted in increasing the original ph of the hydrolysate to 7.0 with cao and reducing it to 5.5 with h3po4.
Detec??o do Sugarcane mosaic virus no Paraná e limpeza somaclonal por cultura de tecidos
Barboza, Ant?nio A.L.;Silva Júnior, Hélio M.;Souto, Eliezer R.;Silva, Clandio M.;Marcuz, Fernanda S.;Vieira, Rafael A.;
Fitopatologia Brasileira , 2007, DOI: 10.1590/S0100-41582007000400010
Abstract: an isolate of sugarcane mosaic virus (scmv) associated with mosaic symptoms in the rb925268 sugarcane clone was identified in paraná state. axilary buds of 4 to 8 mm used as explants were regenerated into plantlets using ms medium with and without ribavirin antiviral, at concentrations of 10 to 60 mg/l. ribavirin was toxic to sugarcane at all concentrations, resulting in plant death at concentrations of 30 mg/l or higher. the complete elimination of scmv was obtained in ms medium with 25 mg/l ribavirin concentration, confirmed by sap inoculation into sorghum bicolor cv. rio and by rt-pcr.
In vitro differential activity of phospholipases and acid proteinases of clinical isolates of Candida
D'E?a Júnior, Aurean;Silva, Anderson Fran?a;Rosa, Fernanda Costa;Monteiro, Sílvio Gomes;Figueiredo, Patrícia de Maria Silva;Monteiro, Cristina de Andrade;
Revista da Sociedade Brasileira de Medicina Tropical , 2011, DOI: 10.1590/S0037-86822011005000036
Abstract: introduction: candida yeasts are commensals; however, if the balance of normal flora is disrupted or the immune defenses are compromised, candida species can cause disease manifestations. several attributes contribute to the virulence and pathogenicity of candida, including the production of extracellular hydrolytic enzymes, particularly phospholipase and proteinase. this study aimed to investigate the in vitro activity of phospholipases and acid proteinases in clinical isolates of candida spp. methods: eighty-two isolates from hospitalized patients collected from various sites of origin were analyzed. phospholipase production was performed in egg yolk medium and the production of proteinase was verified in a medium containing bovine serum albumin. the study was performed in triplicate. results: fifty-six (68.3%) of isolates tested were phospholipase positive and 16 (44.4%) were positive for proteinase activity. c. tropicalis was the species with the highest number of positive isolates for phospholipase (91.7%). statistically significant differences were observed in relation to production of phospholipases among species (p<0,0001) and among the strains from different sites of origin (p=0.014). regarding the production of acid protease, the isolates of c. parapsilosis tested presented a larger number of producers (69.2%). among the species analyzed, the percentage of protease producing isolates did not differ statistically (χ2=1.9 p=0.5901 (χ2=1.9 p=0.5901). conclusions: the majority of c. non-albicans and all c. albicans isolates were great producers of hydrolytic enzymes and, consequently, might be able to cause infection under favorable conditions.
Análise da divergência genética através de caracteres morfológicos em cultivares de feijoeiro Analysis of the genetic divergence through morphologic characters in common bean plant cultivars
Júlio Gomes Júnior,Marco A. A. Barelli,Claudete R. Silva,Leonarda G. Neves
Revista Brasileira de Ciências Agrárias , 2011, DOI: 10.5039/agraria.v6i3a1140
Abstract: A divergência genética entre 22 cultivares de feijoeiro pertencentes ao ensaio de Valor de Cultivo e Uso da Embrapa e IAPAR foi avaliada com o emprego de descritores morfológicos em um experimento de campo conduzido em Cáceres-MT, na unidade experimental da EMPAER. O delineamento experimental utilizado foi o de blocos ao acaso com três repeti es, e os dados obtidos foram submetidos à análise de dissimilaridade genética baseada em variáveis multicategóricas. Empregou-se a análise métodos de agrupamento hierárquico via UPGMA (Método de Agrupamento Médio Entre Grupos) e a proje o da distancia no plano. O emprego de análise de variáveis multicategóricas demonstrou ser eficiente para separar as cultivares em grupos de acordo com os centros de origem mesoamericana e andina. As combina es envolvendo as cultivares BRS Timbó e CNFRx10531; BRS Esplendor e BRS Valente; BRS Grafite e BRS Campeiro se destacaram como as mais similares. As cultivares Iraí e BRS grafite foram as mais divergentes dentre as cultivares avaliadas, portanto, podem ser utilizadas como genitores para a obten o de popula es segregantes superiores em futuros programas de melhoramento genético. The genetic divergence among 22 cultivars of common bean plants, belonging to the assay of Cultivation Value and Use of Embrapa and IAPAR, was evaluated using morphological descriptors in a field experiment in Cáceres, Mato Grosso, Brazil, at the experimental unit of EMPAER. The experimental design was made in randomized blocks, with three replications, and the data obtained were submitted to a genetic dissimilarity analysis based on multicategorical variables. The hierarchical clustering methods analysis was used via UPGMA (Unweighted Pair Group Method with Arithmetic Mean), and the distance projection onto a plane. The use of the multicategorical analysis showed to be efficient to separate the cultivars by groups according to their centers of Mesoamerican and Andean origin. The combinations involving cultivars BRS Timbó and CNFRx10531; BRS Esplendor and BRS Valente; BRS Grafite and BRS Campeiro were the most similar. Cultivars Iraí e BRS Grafite were the most devious among the evaluated cultivars, therefore, they may be used as parents to obtain segregate populations in future breeding programs.
Sistemas de drenagem agrícola. Parte I: desenvolvimento do modelo e análise de sensibilidade
Borges Júnior, Jo?o C. F.;Ferreira, Paulo A.;Pruski, Fernando F.;Silva Júnior, Aziz G. da;
Revista Brasileira de Engenharia Agrícola e Ambiental , 2001, DOI: 10.1590/S1415-43662001000300001
Abstract: this study contemplates the development of a computational model seeking to fill the principal shortcomings of the existing models, and it also accomplishes a sensitivity analysis aiming to verify the effect of the input parameters on the results obtained by application of the model. the model accounts daily for the main components of the water balance in a controled volume at half distance between two parallel drains on unitary base and a height equal to the distance between the soil surface and the soil impermeable layer, simulating the movement of the water table. using the production functions that consider the stress caused by excess and lack of water in the root zone, this model accomplishes the economic analysis for several configurations of the drainage system, allowing for the determination of the economic spacing among drains besides informing about the economic viability of the enterprise. by sensitivity analysis it was verified that the parameters mean drain depth, horizontal soil saturated hydraulic conductivity, drainable porosity, climatic parameters, cn coefficient and the cost of the underground drain installation have a great influence on calculation of the economic drain spacing.
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