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Search Results: 1 - 10 of 18692 matches for " Fengyan Gao "
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Effects of Targeted Suppression of Glutaryl-CoA Dehydrogenase by Lentivirus-Mediated shRNA and Excessive Intake of Lysine on Apoptosis in Rat Striatal Neurons
Jinzhi Gao, Cai Zhang, Xi Fu, Qin Yi, Fengyan Tian, Qin Ning, Xiaoping Luo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0063084
Abstract: In glutaric aciduria type 1 (GA1), glutaryl-CoA dehydrogenase (GCDH) deficiency has been shown to be responsible for the accumulation of glutaric acid and striatal degeneration. However, the mechanisms by which GA1 induces striatal degeneration remain unclear. In this study, we aimed to establish a novel neuronal model of GA1 and to investigate the effects of GCDH deficiency and lysine-related metabolites on the viability of rat striatal neurons. Thus we constructed a lentiviral vector containing short hairpin RNA targeted against the GCDH gene expression (lentivirus-shRNA) in neurons. A virus containing a scrambled short hairpin RNA construct served as a control. Addition of lysine (5 mmol/L) was used to mimic hypermetabolism. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny?ltetrazoliumbromide. Apoptosis was assessed using Hoechst33342 staining and Annexin V-PE/7-AAD staining. The mitochondrial membrane potential (MPP) was monitored using tetramethylrhodamine methyl ester. The expression levels of caspases 3, 8, and 9 were determined by Western blotting. We found that lentivirus-shRNA induced apoptosis and decreased MMP levels in neurons, and addition of 5 mmol/L lysine enhanced this effect markedly. Lentivirus-shRNA upregulated the protein levels of caspases 3 and 9 regardless of the presence of 5 mmol/L lysine. The expression level of caspase 8 was higher in neurons co-treated with lentivirus-shRNA and 5 mmol/L lysine than in control. Benzyloxy-carbonyl-Val-Ala-Asp(OMe)-fluo?romethylketone,a pan-caspase inhibitor, blocked the apoptosis induced by lentivirus-shRNA and 5 mmol/L lysine to a great extent. These results indicate that the targeted suppression of GCDH by lentivirus-mediated shRNA and excessive intake of lysine may be a useful cell model of GA1. These also suggest that GA1-induced striatal degeneration is partially caspase-dependent.
Interior Space Design of Community Activity Center Based on Service Function  [PDF]
Fengyan Shao
Open Journal of Social Sciences (JSS) , 2019, DOI: 10.4236/jss.2019.75017
Abstract: Community activity center is a public space to serve the community masses, which is also the purpose of its existence. The function of community activity center will be adjusted according to the different service population, so the determination of service population and demand is the key to the division and layout of spatial function. This article takes the community service crowd as the breakthrough point, analyzes the different behavior pattern and the use demand of the service crowd, carries on the interior space design to the community activity center based on the service function, promotes the design of public space and the construction of spiritual civilization in the community, and serves the people of the community to live and work in peace and contentment.
A novel differential velocity modulation laser spectroscopy
Yuyan Liu,Hui Gao,Hongping Liu,Chuanxi Duan,Jieli Lin,Yuanqing Guo,Xiaoyong Liu,Guangming Huang,Jinrui Li,Fengyan Li
Chinese Science Bulletin , 2001, DOI: 10.1007/BF03187018
Abstract: Experimental investigation of a novel differential velocity modulation laser spectroscopy is reported and demonstrated with the spectra of n 2 + A2Dm -X2ó g + Meinel system. The S/N ratio excesses 500· ·1, about 60 times higher than that with the traditional non-differential technique. With this technique, we obtained the high-resolution electronic absorption spectra of A2D-X2ó+ (1, 0) vibration-al band of CS+ for the first time. It is confirmed that this technique will be a powerful method and receive wide application in studies of new molecular ions.
A marriage of chemistry and biology

科学通报(英文版) , 2003,
Abstract: 1 Feature: Harvard Institute of Chemistry and Cell Biology (ICCB) One of the major challenges of post-genomic world is how to identify functions of all the proteins encoded by our genome and speed up the process of drug discovery. Harvard Institute of Chemistry and Cell Biology (ICCB) was set up in 1998 to meet this challenge by pursuing a novel field called "Chemical Biology". ICCB is headed by world-renowned chemist Dr. Stuart Schreiber and biologist Dr. Tim Mitchison. Currently ICCB consists of 14 laboratories from the Department of Cell Biology, Harvard Medical School, at Boston and the Department of Chemistry, Harvard University, at Cambridge. Three core facilities, High-throughput Screening, Analytical Chemistry and Biological/Chemical Informatics, are open to the whole Harvard research community for identification of novel chemical ligands for interested biology. ICCB currently employs about 50 researchers and occupies around 10000 sq. ft. of lab space at Harvard Medical School campus. ICCB is supported by grants from NCI and NIGMS as well as by private funding from Merck, Merck KGaA, and the Keck foundation.
Error Estimates of Runge-Kutta Discontinuous Galerkin Methods for the Vlasov-Maxwell System
He Yang,Fengyan Li
Mathematics , 2013,
Abstract: In this paper, error analysis is established for Runge-Kutta discontinuous Galerkin (RKDG) methods to solve the Vlasov-Maxwell system. This nonlinear hyperbolic system describes the time evolution of collisionless plasma particles of a single species under the self-consistent electromagnetic field, and it models many phenomena in both laboratory and astrophysical plasmas. The methods involve a third order TVD Runge-Kutta discretization in time and upwind discontinuous Galerkin discretizations of arbitrary order in phase domain. With the assumption that the exact solution has sufficient regularity, the $L^2$ errors of the particle number density function as well as electric and magnetic fields at any given time $T$ are bounded by $C h^{k+\frac{1}{2}}+C\tau^3$ under a CFL condition $\tau /h \leq \gamma$. Here $k$ is the polynomial degree used in phase space discretization, satisfying $k \geq \left \lceil \frac{d_x + 1}{2} \right \rceil$ (the smallest integer greater than or equal to $\frac{d_x+1}{2}$, with $d_x$ being the dimension of spatial domain), $\tau$ is the time step, and $h$ is the maximum mesh size in phase space. Both $C$ and $\gamma$ are positive constants independent of $h$ and $\tau$, and they may depend on the polynomial degree $k$, time $T$, the size of the phase domain, certain mesh parameters, and some Sobolev norms of the exact solution. The analysis can be extended to RKDG methods with other numerical fluxes and to RKDG methods solving relativistic Vlasov-Maxwell equations.
A novel differential velocity modulation laser spectroscopy

LIU Yuyan,GAO Hui,LIU Hongping,DUAN Chuanxi,LIN Jieli,GUO Yuanqing,LIU Xiaoyong,HUANG Guangming,LI Jinrui,LI Fengyan,

科学通报(英文版) , 2001,
Identification of Genome-Wide Variations among Three Elite Restorer Lines for Hybrid-Rice
Shuangcheng Li, Shiquan Wang, Qiming Deng, Aiping Zheng, Jun Zhu, Huainian Liu, Lingxia Wang, Fengyan Gao, Ting Zou, Bin Huang, Xuemei Cao, Lizhi Xu, Chuang Yu, Peng Ai, Ping Li
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0030952
Abstract: Rice restorer lines play an important role in three-line hybrid rice production. Previous research based on molecular tagging has suggested that the restorer lines used widely today have narrow genetic backgrounds. However, patterns of genetic variation at a genome-wide scale in these restorer lines remain largely unknown. The present study performed re-sequencing and genome-wide variation analysis of three important representative restorer lines, namely, IR24, MH63, and SH527, using the Solexa sequencing technology. With the genomic sequence of the Indica cultivar 9311 as the reference, the following genetic features were identified: 267,383 single-nucleotide polymorphisms (SNPs), 52,847 insertion/deletion polymorphisms (InDels), and 3,286 structural variations (SVs) in the genome of IR24; 288,764 SNPs, 59,658 InDels, and 3,226 SVs in MH63; and 259,862 SNPs, 55,500 InDels, and 3,127 SVs in SH527. Variations between samples were also determined by comparative analysis of authentic collections of SNPs, InDels, and SVs, and were functionally annotated. Furthermore, variations in several important genes were also surveyed by alignment analysis in these lines. Our results suggest that genetic variations among these lines, although far lower than those reported in the landrace population, are greater than expected, indicating a complicated genetic basis for the phenotypic diversity of the restorer lines. Identification of genome-wide variation and pattern analysis among the restorer lines will facilitate future genetic studies and the molecular improvement of hybrid rice.
A three-dimensional diffusion equation of suspended sediment by waves and currents
Pingxing Ding,Fengyan Shi,Yazhen Kong
Chinese Science Bulletin , 1999, DOI: 10.1007/BF02886167
Abstract: Based on the law of mass conservation, a general three-dimensional diffusion equation of suspended sediment due to waves and currents, adaptable to estuarial and coastal areas, is derived by decomposing the instantaneous velocities and concentrations into three-different-time-scale components respectively. A three-dimensional suspended sediment diffusion equation adaptable to actual calculations is available as the result of the parameterizations of turbulent diffusion term and wave diffusion term. Different from the former diffusion equations, the influence of waves and currents on suspended sediment diffusion can be simultaneously reflected in the newly derived equation.
Jian-Ying Huang,Fengyan Zhou
Acta Crystallographica Section E , 2011, DOI: 10.1107/s1600536811008786
Abstract: The title compound, C26H23F2NO3, was synthesized by the reaction of 2-(4-fluorobenzylidene)-4-methyl-3-oxo-N-phenylpentanamide and 4-fluorobenzaldehyde. The dihedral angles between the mean plane through the pyrrolidine ring (nearly planar; maximum deviation of 0.145 for the C atom bearing the hydroxy group) with the phenyl and benzene rings are 37.22 (7), 51.88 (7) and 87.64 (9)°, respectively. The pyyolidine ring is near coplaner, with max offset of 0.145 A for C19 atom.uff09 In the crystal, molecules are linked by pairs of O—H...O hydrogen bonds into inversion dimers, which are further assembled into chains parallel to the b axis by weak C—H...O hydrogen bonds.
Hongqi Li,Yanxi Song,Fengyan Ge
Acta Crystallographica Section E , 2008, DOI: 10.1107/s1600536808005254
Abstract: In the title compound, C21H24O2, rings B, C and D adopt half-chair, distorted half-chair and envelope conformations, respectively. In the crystal structure, there is an intermolecular O—H...O hydrogen bond. The molecules are arranged in a head-to-tail fashion, with the methoxy and hydroxy groups forming a two-dimensional hydrogen-bond network.
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